Job ID = 1293970 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 41,901,421 reads read : 41,901,421 reads written : 41,901,421 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:21:24 41901421 reads; of these: 41901421 (100.00%) were unpaired; of these: 1014126 (2.42%) aligned 0 times 25503665 (60.87%) aligned exactly 1 time 15383630 (36.71%) aligned >1 times 97.58% overall alignment rate Time searching: 00:21:24 Overall time: 00:21:24 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 10780336 / 40887295 = 0.2637 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 03 Jun 2019 04:30:49: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX152102/SRX152102.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX152102/SRX152102.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX152102/SRX152102.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX152102/SRX152102.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 04:30:49: #1 read tag files... INFO @ Mon, 03 Jun 2019 04:30:49: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 04:30:49: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX152102/SRX152102.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX152102/SRX152102.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX152102/SRX152102.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX152102/SRX152102.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 04:30:49: #1 read tag files... INFO @ Mon, 03 Jun 2019 04:30:49: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 04:30:49: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX152102/SRX152102.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX152102/SRX152102.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX152102/SRX152102.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX152102/SRX152102.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 04:30:49: #1 read tag files... INFO @ Mon, 03 Jun 2019 04:30:49: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 04:30:57: 1000000 INFO @ Mon, 03 Jun 2019 04:30:59: 1000000 INFO @ Mon, 03 Jun 2019 04:31:00: 1000000 INFO @ Mon, 03 Jun 2019 04:31:06: 2000000 INFO @ Mon, 03 Jun 2019 04:31:09: 2000000 INFO @ Mon, 03 Jun 2019 04:31:11: 2000000 INFO @ Mon, 03 Jun 2019 04:31:14: 3000000 INFO @ Mon, 03 Jun 2019 04:31:19: 3000000 INFO @ Mon, 03 Jun 2019 04:31:22: 3000000 INFO @ Mon, 03 Jun 2019 04:31:23: 4000000 INFO @ Mon, 03 Jun 2019 04:31:29: 4000000 INFO @ Mon, 03 Jun 2019 04:31:31: 5000000 INFO @ Mon, 03 Jun 2019 04:31:33: 4000000 INFO @ Mon, 03 Jun 2019 04:31:39: 5000000 INFO @ Mon, 03 Jun 2019 04:31:39: 6000000 INFO @ Mon, 03 Jun 2019 04:31:44: 5000000 INFO @ Mon, 03 Jun 2019 04:31:47: 7000000 INFO @ Mon, 03 Jun 2019 04:31:49: 6000000 INFO @ Mon, 03 Jun 2019 04:31:55: 6000000 INFO @ Mon, 03 Jun 2019 04:31:56: 8000000 INFO @ Mon, 03 Jun 2019 04:31:59: 7000000 INFO @ Mon, 03 Jun 2019 04:32:04: 9000000 INFO @ Mon, 03 Jun 2019 04:32:06: 7000000 INFO @ Mon, 03 Jun 2019 04:32:09: 8000000 INFO @ Mon, 03 Jun 2019 04:32:12: 10000000 INFO @ Mon, 03 Jun 2019 04:32:17: 8000000 INFO @ Mon, 03 Jun 2019 04:32:18: 9000000 INFO @ Mon, 03 Jun 2019 04:32:19: 11000000 INFO @ Mon, 03 Jun 2019 04:32:27: 9000000 INFO @ Mon, 03 Jun 2019 04:32:27: 12000000 INFO @ Mon, 03 Jun 2019 04:32:28: 10000000 INFO @ Mon, 03 Jun 2019 04:32:35: 13000000 INFO @ Mon, 03 Jun 2019 04:32:37: 11000000 INFO @ Mon, 03 Jun 2019 04:32:37: 10000000 INFO @ Mon, 03 Jun 2019 04:32:43: 14000000 INFO @ Mon, 03 Jun 2019 04:32:46: 12000000 INFO @ Mon, 03 Jun 2019 04:32:47: 11000000 INFO @ Mon, 03 Jun 2019 04:32:51: 15000000 INFO @ Mon, 03 Jun 2019 04:32:55: 13000000 INFO @ Mon, 03 Jun 2019 04:32:56: 12000000 INFO @ Mon, 03 Jun 2019 04:32:58: 16000000 INFO @ Mon, 03 Jun 2019 04:33:04: 14000000 INFO @ Mon, 03 Jun 2019 04:33:06: 13000000 INFO @ Mon, 03 Jun 2019 04:33:06: 17000000 INFO @ Mon, 03 Jun 2019 04:33:14: 15000000 INFO @ Mon, 03 Jun 2019 04:33:14: 18000000 INFO @ Mon, 03 Jun 2019 04:33:16: 14000000 INFO @ Mon, 03 Jun 2019 04:33:22: 19000000 INFO @ Mon, 03 Jun 2019 04:33:23: 16000000 INFO @ Mon, 03 Jun 2019 04:33:26: 15000000 INFO @ Mon, 03 Jun 2019 04:33:30: 20000000 INFO @ Mon, 03 Jun 2019 04:33:32: 17000000 INFO @ Mon, 03 Jun 2019 04:33:35: 16000000 INFO @ Mon, 03 Jun 2019 04:33:38: 21000000 INFO @ Mon, 03 Jun 2019 04:33:41: 18000000 INFO @ Mon, 03 Jun 2019 04:33:45: 17000000 INFO @ Mon, 03 Jun 2019 04:33:45: 22000000 INFO @ Mon, 03 Jun 2019 04:33:50: 19000000 INFO @ Mon, 03 Jun 2019 04:33:53: 23000000 INFO @ Mon, 03 Jun 2019 04:33:55: 18000000 INFO @ Mon, 03 Jun 2019 04:34:00: 20000000 INFO @ Mon, 03 Jun 2019 04:34:01: 24000000 INFO @ Mon, 03 Jun 2019 04:34:04: 19000000 INFO @ Mon, 03 Jun 2019 04:34:09: 21000000 INFO @ Mon, 03 Jun 2019 04:34:09: 25000000 INFO @ Mon, 03 Jun 2019 04:34:14: 20000000 INFO @ Mon, 03 Jun 2019 04:34:18: 26000000 INFO @ Mon, 03 Jun 2019 04:34:18: 22000000 INFO @ Mon, 03 Jun 2019 04:34:24: 21000000 INFO @ Mon, 03 Jun 2019 04:34:26: 27000000 INFO @ Mon, 03 Jun 2019 04:34:27: 23000000 INFO @ Mon, 03 Jun 2019 04:34:33: 22000000 INFO @ Mon, 03 Jun 2019 04:34:34: 28000000 INFO @ Mon, 03 Jun 2019 04:34:37: 24000000 INFO @ Mon, 03 Jun 2019 04:34:42: 29000000 INFO @ Mon, 03 Jun 2019 04:34:43: 23000000 INFO @ Mon, 03 Jun 2019 04:34:47: 25000000 INFO @ Mon, 03 Jun 2019 04:34:50: 30000000 INFO @ Mon, 03 Jun 2019 04:34:51: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 04:34:51: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 04:34:51: #1 total tags in treatment: 30106959 INFO @ Mon, 03 Jun 2019 04:34:51: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 04:34:51: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 04:34:51: #1 tags after filtering in treatment: 30106959 INFO @ Mon, 03 Jun 2019 04:34:51: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 04:34:51: #1 finished! INFO @ Mon, 03 Jun 2019 04:34:51: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 04:34:51: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 04:34:52: 24000000 INFO @ Mon, 03 Jun 2019 04:34:54: #2 number of paired peaks: 0 WARNING @ Mon, 03 Jun 2019 04:34:54: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 04:34:54: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX152102/SRX152102.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX152102/SRX152102.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX152102/SRX152102.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX152102/SRX152102.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 04:34:57: 26000000 INFO @ Mon, 03 Jun 2019 04:35:02: 25000000 INFO @ Mon, 03 Jun 2019 04:35:06: 27000000 INFO @ Mon, 03 Jun 2019 04:35:13: 26000000 INFO @ Mon, 03 Jun 2019 04:35:16: 28000000 INFO @ Mon, 03 Jun 2019 04:35:22: 27000000 INFO @ Mon, 03 Jun 2019 04:35:25: 29000000 INFO @ Mon, 03 Jun 2019 04:35:32: 28000000 INFO @ Mon, 03 Jun 2019 04:35:34: 30000000 INFO @ Mon, 03 Jun 2019 04:35:35: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 04:35:35: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 04:35:35: #1 total tags in treatment: 30106959 INFO @ Mon, 03 Jun 2019 04:35:35: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 04:35:35: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 04:35:36: #1 tags after filtering in treatment: 30106959 INFO @ Mon, 03 Jun 2019 04:35:36: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 04:35:36: #1 finished! INFO @ Mon, 03 Jun 2019 04:35:36: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 04:35:36: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 04:35:38: #2 number of paired peaks: 0 WARNING @ Mon, 03 Jun 2019 04:35:38: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 04:35:38: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX152102/SRX152102.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX152102/SRX152102.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX152102/SRX152102.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX152102/SRX152102.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 04:35:42: 29000000 INFO @ Mon, 03 Jun 2019 04:35:51: 30000000 INFO @ Mon, 03 Jun 2019 04:35:52: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 04:35:52: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 04:35:52: #1 total tags in treatment: 30106959 INFO @ Mon, 03 Jun 2019 04:35:52: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 04:35:52: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 04:35:53: #1 tags after filtering in treatment: 30106959 INFO @ Mon, 03 Jun 2019 04:35:53: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 04:35:53: #1 finished! INFO @ Mon, 03 Jun 2019 04:35:53: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 04:35:53: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 04:35:55: #2 number of paired peaks: 0 WARNING @ Mon, 03 Jun 2019 04:35:55: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 04:35:55: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX152102/SRX152102.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX152102/SRX152102.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX152102/SRX152102.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX152102/SRX152102.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。