Job ID = 1293969 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 29,985,092 reads read : 29,985,092 reads written : 29,985,092 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:11:58 29985092 reads; of these: 29985092 (100.00%) were unpaired; of these: 416043 (1.39%) aligned 0 times 21924191 (73.12%) aligned exactly 1 time 7644858 (25.50%) aligned >1 times 98.61% overall alignment rate Time searching: 00:11:58 Overall time: 00:11:58 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 6691522 / 29569049 = 0.2263 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 03 Jun 2019 03:58:47: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX152101/SRX152101.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX152101/SRX152101.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX152101/SRX152101.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX152101/SRX152101.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 03:58:47: #1 read tag files... INFO @ Mon, 03 Jun 2019 03:58:47: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 03:58:47: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX152101/SRX152101.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX152101/SRX152101.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX152101/SRX152101.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX152101/SRX152101.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 03:58:47: #1 read tag files... INFO @ Mon, 03 Jun 2019 03:58:47: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 03:58:47: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX152101/SRX152101.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX152101/SRX152101.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX152101/SRX152101.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX152101/SRX152101.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 03:58:47: #1 read tag files... INFO @ Mon, 03 Jun 2019 03:58:47: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 03:58:56: 1000000 INFO @ Mon, 03 Jun 2019 03:58:58: 1000000 INFO @ Mon, 03 Jun 2019 03:58:59: 1000000 INFO @ Mon, 03 Jun 2019 03:59:04: 2000000 INFO @ Mon, 03 Jun 2019 03:59:08: 2000000 INFO @ Mon, 03 Jun 2019 03:59:09: 2000000 INFO @ Mon, 03 Jun 2019 03:59:12: 3000000 INFO @ Mon, 03 Jun 2019 03:59:19: 3000000 INFO @ Mon, 03 Jun 2019 03:59:20: 3000000 INFO @ Mon, 03 Jun 2019 03:59:21: 4000000 INFO @ Mon, 03 Jun 2019 03:59:29: 5000000 INFO @ Mon, 03 Jun 2019 03:59:30: 4000000 INFO @ Mon, 03 Jun 2019 03:59:31: 4000000 INFO @ Mon, 03 Jun 2019 03:59:37: 6000000 INFO @ Mon, 03 Jun 2019 03:59:40: 5000000 INFO @ Mon, 03 Jun 2019 03:59:42: 5000000 INFO @ Mon, 03 Jun 2019 03:59:46: 7000000 INFO @ Mon, 03 Jun 2019 03:59:52: 6000000 INFO @ Mon, 03 Jun 2019 03:59:54: 6000000 INFO @ Mon, 03 Jun 2019 03:59:54: 8000000 INFO @ Mon, 03 Jun 2019 04:00:02: 9000000 INFO @ Mon, 03 Jun 2019 04:00:03: 7000000 INFO @ Mon, 03 Jun 2019 04:00:05: 7000000 INFO @ Mon, 03 Jun 2019 04:00:11: 10000000 INFO @ Mon, 03 Jun 2019 04:00:13: 8000000 INFO @ Mon, 03 Jun 2019 04:00:16: 8000000 INFO @ Mon, 03 Jun 2019 04:00:19: 11000000 INFO @ Mon, 03 Jun 2019 04:00:24: 9000000 INFO @ Mon, 03 Jun 2019 04:00:27: 9000000 INFO @ Mon, 03 Jun 2019 04:00:27: 12000000 INFO @ Mon, 03 Jun 2019 04:00:34: 10000000 INFO @ Mon, 03 Jun 2019 04:00:36: 13000000 INFO @ Mon, 03 Jun 2019 04:00:37: 10000000 INFO @ Mon, 03 Jun 2019 04:00:44: 11000000 INFO @ Mon, 03 Jun 2019 04:00:44: 14000000 INFO @ Mon, 03 Jun 2019 04:00:48: 11000000 INFO @ Mon, 03 Jun 2019 04:00:53: 15000000 INFO @ Mon, 03 Jun 2019 04:00:54: 12000000 INFO @ Mon, 03 Jun 2019 04:00:59: 12000000 INFO @ Mon, 03 Jun 2019 04:01:02: 16000000 INFO @ Mon, 03 Jun 2019 04:01:04: 13000000 INFO @ Mon, 03 Jun 2019 04:01:09: 13000000 INFO @ Mon, 03 Jun 2019 04:01:10: 17000000 INFO @ Mon, 03 Jun 2019 04:01:14: 14000000 INFO @ Mon, 03 Jun 2019 04:01:18: 18000000 INFO @ Mon, 03 Jun 2019 04:01:20: 14000000 INFO @ Mon, 03 Jun 2019 04:01:24: 15000000 INFO @ Mon, 03 Jun 2019 04:01:26: 19000000 INFO @ Mon, 03 Jun 2019 04:01:29: 15000000 INFO @ Mon, 03 Jun 2019 04:01:34: 20000000 INFO @ Mon, 03 Jun 2019 04:01:35: 16000000 INFO @ Mon, 03 Jun 2019 04:01:39: 16000000 INFO @ Mon, 03 Jun 2019 04:01:43: 21000000 INFO @ Mon, 03 Jun 2019 04:01:45: 17000000 INFO @ Mon, 03 Jun 2019 04:01:50: 17000000 INFO @ Mon, 03 Jun 2019 04:01:51: 22000000 INFO @ Mon, 03 Jun 2019 04:01:56: 18000000 INFO @ Mon, 03 Jun 2019 04:01:59: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 04:01:59: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 04:01:59: #1 total tags in treatment: 22877527 INFO @ Mon, 03 Jun 2019 04:01:59: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 04:01:59: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 04:01:59: #1 tags after filtering in treatment: 22877527 INFO @ Mon, 03 Jun 2019 04:01:59: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 04:01:59: #1 finished! INFO @ Mon, 03 Jun 2019 04:01:59: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 04:01:59: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 04:02:01: 18000000 INFO @ Mon, 03 Jun 2019 04:02:01: #2 number of paired peaks: 1 WARNING @ Mon, 03 Jun 2019 04:02:01: Too few paired peaks (1) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 04:02:01: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX152101/SRX152101.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX152101/SRX152101.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX152101/SRX152101.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX152101/SRX152101.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 04:02:05: 19000000 INFO @ Mon, 03 Jun 2019 04:02:11: 19000000 INFO @ Mon, 03 Jun 2019 04:02:14: 20000000 INFO @ Mon, 03 Jun 2019 04:02:21: 20000000 INFO @ Mon, 03 Jun 2019 04:02:22: 21000000 INFO @ Mon, 03 Jun 2019 04:02:31: 22000000 INFO @ Mon, 03 Jun 2019 04:02:32: 21000000 INFO @ Mon, 03 Jun 2019 04:02:39: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 04:02:39: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 04:02:39: #1 total tags in treatment: 22877527 INFO @ Mon, 03 Jun 2019 04:02:39: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 04:02:39: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 04:02:39: #1 tags after filtering in treatment: 22877527 INFO @ Mon, 03 Jun 2019 04:02:39: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 04:02:39: #1 finished! INFO @ Mon, 03 Jun 2019 04:02:39: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 04:02:39: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 04:02:41: #2 number of paired peaks: 1 WARNING @ Mon, 03 Jun 2019 04:02:41: Too few paired peaks (1) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 04:02:41: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX152101/SRX152101.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX152101/SRX152101.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX152101/SRX152101.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX152101/SRX152101.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 04:02:43: 22000000 INFO @ Mon, 03 Jun 2019 04:02:53: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 04:02:53: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 04:02:53: #1 total tags in treatment: 22877527 INFO @ Mon, 03 Jun 2019 04:02:53: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 04:02:53: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 04:02:53: #1 tags after filtering in treatment: 22877527 INFO @ Mon, 03 Jun 2019 04:02:53: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 04:02:53: #1 finished! INFO @ Mon, 03 Jun 2019 04:02:53: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 04:02:53: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 04:02:55: #2 number of paired peaks: 1 WARNING @ Mon, 03 Jun 2019 04:02:55: Too few paired peaks (1) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 04:02:55: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX152101/SRX152101.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX152101/SRX152101.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX152101/SRX152101.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX152101/SRX152101.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。