Job ID = 16438953 SRX = SRX15206526 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2022-08-02T05:14:54 prefetch.2.10.7: 1) Downloading 'SRR19139266'... 2022-08-02T05:14:54 prefetch.2.10.7: Downloading via HTTPS... 2022-08-02T05:15:39 prefetch.2.10.7: HTTPS download succeed 2022-08-02T05:15:39 prefetch.2.10.7: 1) 'SRR19139266' was downloaded successfully 2022-08-02T05:15:39 prefetch.2.10.7: 'SRR19139266' has 0 unresolved dependencies Read 12722405 spots for SRR19139266/SRR19139266.sra Written 12722405 spots for SRR19139266/SRR19139266.sra fastq に変換しました。 bowtie でマッピング中... Your job 16439137 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:01 Multiseed full-index search: 00:10:09 12722405 reads; of these: 12722405 (100.00%) were unpaired; of these: 851342 (6.69%) aligned 0 times 10021015 (78.77%) aligned exactly 1 time 1850048 (14.54%) aligned >1 times 93.31% overall alignment rate Time searching: 00:10:10 Overall time: 00:10:10 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 3225338 / 11871063 = 0.2717 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 14:35:20: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX15206526/SRX15206526.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX15206526/SRX15206526.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX15206526/SRX15206526.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX15206526/SRX15206526.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 14:35:20: #1 read tag files... INFO @ Tue, 02 Aug 2022 14:35:20: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 14:35:29: 1000000 INFO @ Tue, 02 Aug 2022 14:35:38: 2000000 INFO @ Tue, 02 Aug 2022 14:35:48: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 14:35:50: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX15206526/SRX15206526.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX15206526/SRX15206526.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX15206526/SRX15206526.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX15206526/SRX15206526.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 14:35:50: #1 read tag files... INFO @ Tue, 02 Aug 2022 14:35:50: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 14:35:58: 4000000 INFO @ Tue, 02 Aug 2022 14:36:00: 1000000 INFO @ Tue, 02 Aug 2022 14:36:08: 5000000 INFO @ Tue, 02 Aug 2022 14:36:10: 2000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 14:36:18: 6000000 INFO @ Tue, 02 Aug 2022 14:36:20: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX15206526/SRX15206526.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX15206526/SRX15206526.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX15206526/SRX15206526.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX15206526/SRX15206526.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 14:36:20: #1 read tag files... INFO @ Tue, 02 Aug 2022 14:36:20: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 14:36:20: 3000000 INFO @ Tue, 02 Aug 2022 14:36:28: 7000000 INFO @ Tue, 02 Aug 2022 14:36:30: 1000000 INFO @ Tue, 02 Aug 2022 14:36:31: 4000000 INFO @ Tue, 02 Aug 2022 14:36:39: 8000000 INFO @ Tue, 02 Aug 2022 14:36:41: 5000000 INFO @ Tue, 02 Aug 2022 14:36:41: 2000000 INFO @ Tue, 02 Aug 2022 14:36:46: #1 tag size is determined as 150 bps INFO @ Tue, 02 Aug 2022 14:36:46: #1 tag size = 150 INFO @ Tue, 02 Aug 2022 14:36:46: #1 total tags in treatment: 8645725 INFO @ Tue, 02 Aug 2022 14:36:46: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 14:36:46: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 14:36:46: #1 tags after filtering in treatment: 8645725 INFO @ Tue, 02 Aug 2022 14:36:46: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 14:36:46: #1 finished! INFO @ Tue, 02 Aug 2022 14:36:46: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 14:36:46: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 14:36:47: #2 number of paired peaks: 85 WARNING @ Tue, 02 Aug 2022 14:36:47: Too few paired peaks (85) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 14:36:47: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX15206526/SRX15206526.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX15206526/SRX15206526.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX15206526/SRX15206526.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX15206526/SRX15206526.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 14:36:51: 6000000 INFO @ Tue, 02 Aug 2022 14:36:51: 3000000 INFO @ Tue, 02 Aug 2022 14:37:00: 4000000 INFO @ Tue, 02 Aug 2022 14:37:00: 7000000 INFO @ Tue, 02 Aug 2022 14:37:10: 5000000 INFO @ Tue, 02 Aug 2022 14:37:10: 8000000 INFO @ Tue, 02 Aug 2022 14:37:17: #1 tag size is determined as 150 bps INFO @ Tue, 02 Aug 2022 14:37:17: #1 tag size = 150 INFO @ Tue, 02 Aug 2022 14:37:17: #1 total tags in treatment: 8645725 INFO @ Tue, 02 Aug 2022 14:37:17: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 14:37:17: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 14:37:17: #1 tags after filtering in treatment: 8645725 INFO @ Tue, 02 Aug 2022 14:37:17: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 14:37:17: #1 finished! INFO @ Tue, 02 Aug 2022 14:37:17: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 14:37:17: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 14:37:18: #2 number of paired peaks: 85 WARNING @ Tue, 02 Aug 2022 14:37:18: Too few paired peaks (85) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 14:37:18: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX15206526/SRX15206526.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX15206526/SRX15206526.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX15206526/SRX15206526.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX15206526/SRX15206526.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 14:37:19: 6000000 INFO @ Tue, 02 Aug 2022 14:37:28: 7000000 INFO @ Tue, 02 Aug 2022 14:37:36: 8000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 02 Aug 2022 14:37:42: #1 tag size is determined as 150 bps INFO @ Tue, 02 Aug 2022 14:37:42: #1 tag size = 150 INFO @ Tue, 02 Aug 2022 14:37:42: #1 total tags in treatment: 8645725 INFO @ Tue, 02 Aug 2022 14:37:42: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 14:37:42: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 14:37:42: #1 tags after filtering in treatment: 8645725 INFO @ Tue, 02 Aug 2022 14:37:42: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 14:37:42: #1 finished! INFO @ Tue, 02 Aug 2022 14:37:42: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 14:37:42: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 14:37:43: #2 number of paired peaks: 85 WARNING @ Tue, 02 Aug 2022 14:37:43: Too few paired peaks (85) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 14:37:43: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX15206526/SRX15206526.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX15206526/SRX15206526.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX15206526/SRX15206526.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX15206526/SRX15206526.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。