Job ID = 16438916 SRX = SRX15206517 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2022-08-02T05:08:54 prefetch.2.10.7: 1) Downloading 'SRR19139275'... 2022-08-02T05:08:55 prefetch.2.10.7: Downloading via HTTPS... 2022-08-02T05:09:16 prefetch.2.10.7: HTTPS download succeed 2022-08-02T05:09:16 prefetch.2.10.7: 'SRR19139275' is valid 2022-08-02T05:09:16 prefetch.2.10.7: 1) 'SRR19139275' was downloaded successfully 2022-08-02T05:09:16 prefetch.2.10.7: 'SRR19139275' has 0 unresolved dependencies Read 10338010 spots for SRR19139275/SRR19139275.sra Written 10338010 spots for SRR19139275/SRR19139275.sra fastq に変換しました。 bowtie でマッピング中... Your job 16438968 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:33 10338010 reads; of these: 10338010 (100.00%) were unpaired; of these: 479905 (4.64%) aligned 0 times 8700109 (84.16%) aligned exactly 1 time 1157996 (11.20%) aligned >1 times 95.36% overall alignment rate Time searching: 00:04:33 Overall time: 00:04:33 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 1901352 / 9858105 = 0.1929 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 14:17:20: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX15206517/SRX15206517.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX15206517/SRX15206517.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX15206517/SRX15206517.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX15206517/SRX15206517.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 14:17:20: #1 read tag files... INFO @ Tue, 02 Aug 2022 14:17:20: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 14:17:28: 1000000 INFO @ Tue, 02 Aug 2022 14:17:36: 2000000 INFO @ Tue, 02 Aug 2022 14:17:43: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 14:17:50: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX15206517/SRX15206517.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX15206517/SRX15206517.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX15206517/SRX15206517.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX15206517/SRX15206517.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 14:17:50: #1 read tag files... INFO @ Tue, 02 Aug 2022 14:17:50: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 14:17:51: 4000000 INFO @ Tue, 02 Aug 2022 14:17:58: 1000000 INFO @ Tue, 02 Aug 2022 14:17:59: 5000000 INFO @ Tue, 02 Aug 2022 14:18:05: 2000000 INFO @ Tue, 02 Aug 2022 14:18:07: 6000000 INFO @ Tue, 02 Aug 2022 14:18:12: 3000000 INFO @ Tue, 02 Aug 2022 14:18:15: 7000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 14:18:19: 4000000 INFO @ Tue, 02 Aug 2022 14:18:21: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX15206517/SRX15206517.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX15206517/SRX15206517.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX15206517/SRX15206517.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX15206517/SRX15206517.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 14:18:21: #1 read tag files... INFO @ Tue, 02 Aug 2022 14:18:21: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 14:18:23: #1 tag size is determined as 100 bps INFO @ Tue, 02 Aug 2022 14:18:23: #1 tag size = 100 INFO @ Tue, 02 Aug 2022 14:18:23: #1 total tags in treatment: 7956753 INFO @ Tue, 02 Aug 2022 14:18:23: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 14:18:23: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 14:18:23: #1 tags after filtering in treatment: 7956753 INFO @ Tue, 02 Aug 2022 14:18:23: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 14:18:23: #1 finished! INFO @ Tue, 02 Aug 2022 14:18:23: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 14:18:23: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 14:18:23: #2 number of paired peaks: 74 WARNING @ Tue, 02 Aug 2022 14:18:23: Too few paired peaks (74) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 14:18:23: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX15206517/SRX15206517.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX15206517/SRX15206517.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX15206517/SRX15206517.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX15206517/SRX15206517.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 14:18:26: 5000000 INFO @ Tue, 02 Aug 2022 14:18:29: 1000000 INFO @ Tue, 02 Aug 2022 14:18:32: 6000000 INFO @ Tue, 02 Aug 2022 14:18:37: 2000000 INFO @ Tue, 02 Aug 2022 14:18:39: 7000000 INFO @ Tue, 02 Aug 2022 14:18:45: 3000000 INFO @ Tue, 02 Aug 2022 14:18:46: #1 tag size is determined as 100 bps INFO @ Tue, 02 Aug 2022 14:18:46: #1 tag size = 100 INFO @ Tue, 02 Aug 2022 14:18:46: #1 total tags in treatment: 7956753 INFO @ Tue, 02 Aug 2022 14:18:46: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 14:18:46: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 14:18:46: #1 tags after filtering in treatment: 7956753 INFO @ Tue, 02 Aug 2022 14:18:46: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 14:18:46: #1 finished! INFO @ Tue, 02 Aug 2022 14:18:46: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 14:18:46: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 14:18:46: #2 number of paired peaks: 74 WARNING @ Tue, 02 Aug 2022 14:18:46: Too few paired peaks (74) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 14:18:46: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX15206517/SRX15206517.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX15206517/SRX15206517.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX15206517/SRX15206517.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX15206517/SRX15206517.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 14:18:53: 4000000 INFO @ Tue, 02 Aug 2022 14:19:00: 5000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 02 Aug 2022 14:19:07: 6000000 INFO @ Tue, 02 Aug 2022 14:19:15: 7000000 BigWig に変換しました。 INFO @ Tue, 02 Aug 2022 14:19:22: #1 tag size is determined as 100 bps INFO @ Tue, 02 Aug 2022 14:19:22: #1 tag size = 100 INFO @ Tue, 02 Aug 2022 14:19:22: #1 total tags in treatment: 7956753 INFO @ Tue, 02 Aug 2022 14:19:22: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 14:19:22: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 14:19:22: #1 tags after filtering in treatment: 7956753 INFO @ Tue, 02 Aug 2022 14:19:22: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 14:19:22: #1 finished! INFO @ Tue, 02 Aug 2022 14:19:22: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 14:19:22: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 14:19:23: #2 number of paired peaks: 74 WARNING @ Tue, 02 Aug 2022 14:19:23: Too few paired peaks (74) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 14:19:23: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX15206517/SRX15206517.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX15206517/SRX15206517.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX15206517/SRX15206517.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX15206517/SRX15206517.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling