Job ID = 16438911 SRX = SRX15206514 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2022-08-02T05:07:41 prefetch.2.10.7: 1) Downloading 'SRR19139278'... 2022-08-02T05:07:41 prefetch.2.10.7: Downloading via HTTPS... 2022-08-02T05:07:58 prefetch.2.10.7: HTTPS download succeed 2022-08-02T05:07:59 prefetch.2.10.7: 'SRR19139278' is valid 2022-08-02T05:07:59 prefetch.2.10.7: 1) 'SRR19139278' was downloaded successfully 2022-08-02T05:07:59 prefetch.2.10.7: 'SRR19139278' has 0 unresolved dependencies Read 15583647 spots for SRR19139278/SRR19139278.sra Written 15583647 spots for SRR19139278/SRR19139278.sra fastq に変換しました。 bowtie でマッピング中... Your job 16438956 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:55 15583647 reads; of these: 15583647 (100.00%) were unpaired; of these: 426194 (2.73%) aligned 0 times 13489033 (86.56%) aligned exactly 1 time 1668420 (10.71%) aligned >1 times 97.27% overall alignment rate Time searching: 00:03:56 Overall time: 00:03:56 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 980295 / 15157453 = 0.0647 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 14:15:50: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX15206514/SRX15206514.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX15206514/SRX15206514.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX15206514/SRX15206514.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX15206514/SRX15206514.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 14:15:50: #1 read tag files... INFO @ Tue, 02 Aug 2022 14:15:50: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 14:15:56: 1000000 INFO @ Tue, 02 Aug 2022 14:16:03: 2000000 INFO @ Tue, 02 Aug 2022 14:16:09: 3000000 INFO @ Tue, 02 Aug 2022 14:16:16: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 14:16:20: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX15206514/SRX15206514.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX15206514/SRX15206514.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX15206514/SRX15206514.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX15206514/SRX15206514.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 14:16:20: #1 read tag files... INFO @ Tue, 02 Aug 2022 14:16:20: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 14:16:23: 5000000 INFO @ Tue, 02 Aug 2022 14:16:28: 1000000 INFO @ Tue, 02 Aug 2022 14:16:30: 6000000 INFO @ Tue, 02 Aug 2022 14:16:35: 2000000 INFO @ Tue, 02 Aug 2022 14:16:37: 7000000 INFO @ Tue, 02 Aug 2022 14:16:42: 3000000 INFO @ Tue, 02 Aug 2022 14:16:44: 8000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 14:16:49: 4000000 INFO @ Tue, 02 Aug 2022 14:16:50: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX15206514/SRX15206514.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX15206514/SRX15206514.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX15206514/SRX15206514.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX15206514/SRX15206514.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 14:16:50: #1 read tag files... INFO @ Tue, 02 Aug 2022 14:16:50: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 14:16:51: 9000000 INFO @ Tue, 02 Aug 2022 14:16:56: 5000000 INFO @ Tue, 02 Aug 2022 14:16:57: 1000000 INFO @ Tue, 02 Aug 2022 14:16:58: 10000000 INFO @ Tue, 02 Aug 2022 14:17:04: 6000000 INFO @ Tue, 02 Aug 2022 14:17:04: 2000000 INFO @ Tue, 02 Aug 2022 14:17:05: 11000000 INFO @ Tue, 02 Aug 2022 14:17:11: 7000000 INFO @ Tue, 02 Aug 2022 14:17:11: 3000000 INFO @ Tue, 02 Aug 2022 14:17:12: 12000000 INFO @ Tue, 02 Aug 2022 14:17:18: 8000000 INFO @ Tue, 02 Aug 2022 14:17:18: 4000000 INFO @ Tue, 02 Aug 2022 14:17:19: 13000000 INFO @ Tue, 02 Aug 2022 14:17:25: 9000000 INFO @ Tue, 02 Aug 2022 14:17:25: 5000000 INFO @ Tue, 02 Aug 2022 14:17:27: 14000000 INFO @ Tue, 02 Aug 2022 14:17:28: #1 tag size is determined as 50 bps INFO @ Tue, 02 Aug 2022 14:17:28: #1 tag size = 50 INFO @ Tue, 02 Aug 2022 14:17:28: #1 total tags in treatment: 14177158 INFO @ Tue, 02 Aug 2022 14:17:28: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 14:17:28: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 14:17:28: #1 tags after filtering in treatment: 14177158 INFO @ Tue, 02 Aug 2022 14:17:28: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 14:17:28: #1 finished! INFO @ Tue, 02 Aug 2022 14:17:28: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 14:17:28: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 14:17:29: #2 number of paired peaks: 65 WARNING @ Tue, 02 Aug 2022 14:17:29: Too few paired peaks (65) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 14:17:29: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX15206514/SRX15206514.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX15206514/SRX15206514.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX15206514/SRX15206514.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX15206514/SRX15206514.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 14:17:32: 10000000 INFO @ Tue, 02 Aug 2022 14:17:32: 6000000 INFO @ Tue, 02 Aug 2022 14:17:39: 11000000 INFO @ Tue, 02 Aug 2022 14:17:39: 7000000 INFO @ Tue, 02 Aug 2022 14:17:45: 12000000 INFO @ Tue, 02 Aug 2022 14:17:46: 8000000 INFO @ Tue, 02 Aug 2022 14:17:52: 13000000 INFO @ Tue, 02 Aug 2022 14:17:53: 9000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 02 Aug 2022 14:17:59: 14000000 INFO @ Tue, 02 Aug 2022 14:18:00: 10000000 INFO @ Tue, 02 Aug 2022 14:18:00: #1 tag size is determined as 50 bps INFO @ Tue, 02 Aug 2022 14:18:00: #1 tag size = 50 INFO @ Tue, 02 Aug 2022 14:18:00: #1 total tags in treatment: 14177158 INFO @ Tue, 02 Aug 2022 14:18:00: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 14:18:00: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 14:18:01: #1 tags after filtering in treatment: 14177158 INFO @ Tue, 02 Aug 2022 14:18:01: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 14:18:01: #1 finished! INFO @ Tue, 02 Aug 2022 14:18:01: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 14:18:01: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 14:18:02: #2 number of paired peaks: 65 WARNING @ Tue, 02 Aug 2022 14:18:02: Too few paired peaks (65) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 14:18:02: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX15206514/SRX15206514.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX15206514/SRX15206514.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX15206514/SRX15206514.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX15206514/SRX15206514.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 14:18:06: 11000000 INFO @ Tue, 02 Aug 2022 14:18:13: 12000000 INFO @ Tue, 02 Aug 2022 14:18:19: 13000000 INFO @ Tue, 02 Aug 2022 14:18:25: 14000000 INFO @ Tue, 02 Aug 2022 14:18:26: #1 tag size is determined as 50 bps INFO @ Tue, 02 Aug 2022 14:18:26: #1 tag size = 50 INFO @ Tue, 02 Aug 2022 14:18:26: #1 total tags in treatment: 14177158 INFO @ Tue, 02 Aug 2022 14:18:26: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 14:18:26: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 14:18:26: #1 tags after filtering in treatment: 14177158 INFO @ Tue, 02 Aug 2022 14:18:26: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 14:18:26: #1 finished! INFO @ Tue, 02 Aug 2022 14:18:26: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 14:18:26: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 14:18:27: #2 number of paired peaks: 65 WARNING @ Tue, 02 Aug 2022 14:18:27: Too few paired peaks (65) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 14:18:27: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX15206514/SRX15206514.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX15206514/SRX15206514.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX15206514/SRX15206514.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX15206514/SRX15206514.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。