Job ID = 16439023 SRX = SRX15206449 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2022-08-02T05:26:02 prefetch.2.10.7: 1) Downloading 'SRR19139358'... 2022-08-02T05:26:02 prefetch.2.10.7: Downloading via HTTPS... 2022-08-02T05:26:53 prefetch.2.10.7: HTTPS download succeed 2022-08-02T05:26:53 prefetch.2.10.7: 1) 'SRR19139358' was downloaded successfully 2022-08-02T05:26:53 prefetch.2.10.7: 'SRR19139358' has 0 unresolved dependencies Read 14131382 spots for SRR19139358/SRR19139358.sra Written 14131382 spots for SRR19139358/SRR19139358.sra 2022-08-02T05:27:51 prefetch.2.10.7: 1) Downloading 'SRR19139359'... 2022-08-02T05:27:51 prefetch.2.10.7: Downloading via HTTPS... 2022-08-02T05:28:38 prefetch.2.10.7: HTTPS download succeed 2022-08-02T05:28:38 prefetch.2.10.7: 1) 'SRR19139359' was downloaded successfully 2022-08-02T05:28:38 prefetch.2.10.7: 'SRR19139359' has 0 unresolved dependencies Read 14131382 spots for SRR19139359/SRR19139359.sra Written 14131382 spots for SRR19139359/SRR19139359.sra fastq に変換しました。 bowtie でマッピング中... Your job 16439293 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:19:17 28262764 reads; of these: 28262764 (100.00%) were unpaired; of these: 1272158 (4.50%) aligned 0 times 22815984 (80.73%) aligned exactly 1 time 4174622 (14.77%) aligned >1 times 95.50% overall alignment rate Time searching: 00:19:17 Overall time: 00:19:17 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 8299712 / 26990606 = 0.3075 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 15:00:23: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX15206449/SRX15206449.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX15206449/SRX15206449.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX15206449/SRX15206449.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX15206449/SRX15206449.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 15:00:23: #1 read tag files... INFO @ Tue, 02 Aug 2022 15:00:23: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 15:00:30: 1000000 INFO @ Tue, 02 Aug 2022 15:00:37: 2000000 INFO @ Tue, 02 Aug 2022 15:00:44: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 15:00:51: 4000000 INFO @ Tue, 02 Aug 2022 15:00:52: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX15206449/SRX15206449.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX15206449/SRX15206449.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX15206449/SRX15206449.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX15206449/SRX15206449.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 15:00:52: #1 read tag files... INFO @ Tue, 02 Aug 2022 15:00:52: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 15:00:58: 5000000 INFO @ Tue, 02 Aug 2022 15:00:59: 1000000 INFO @ Tue, 02 Aug 2022 15:01:05: 6000000 INFO @ Tue, 02 Aug 2022 15:01:07: 2000000 INFO @ Tue, 02 Aug 2022 15:01:13: 7000000 INFO @ Tue, 02 Aug 2022 15:01:14: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 15:01:20: 8000000 INFO @ Tue, 02 Aug 2022 15:01:21: 4000000 INFO @ Tue, 02 Aug 2022 15:01:22: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX15206449/SRX15206449.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX15206449/SRX15206449.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX15206449/SRX15206449.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX15206449/SRX15206449.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 15:01:22: #1 read tag files... INFO @ Tue, 02 Aug 2022 15:01:22: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 15:01:28: 9000000 INFO @ Tue, 02 Aug 2022 15:01:28: 5000000 INFO @ Tue, 02 Aug 2022 15:01:29: 1000000 INFO @ Tue, 02 Aug 2022 15:01:35: 10000000 INFO @ Tue, 02 Aug 2022 15:01:36: 6000000 INFO @ Tue, 02 Aug 2022 15:01:37: 2000000 INFO @ Tue, 02 Aug 2022 15:01:43: 11000000 INFO @ Tue, 02 Aug 2022 15:01:43: 7000000 INFO @ Tue, 02 Aug 2022 15:01:44: 3000000 INFO @ Tue, 02 Aug 2022 15:01:50: 12000000 INFO @ Tue, 02 Aug 2022 15:01:50: 8000000 INFO @ Tue, 02 Aug 2022 15:01:52: 4000000 INFO @ Tue, 02 Aug 2022 15:01:58: 13000000 INFO @ Tue, 02 Aug 2022 15:01:58: 9000000 INFO @ Tue, 02 Aug 2022 15:02:00: 5000000 INFO @ Tue, 02 Aug 2022 15:02:06: 10000000 INFO @ Tue, 02 Aug 2022 15:02:06: 14000000 INFO @ Tue, 02 Aug 2022 15:02:08: 6000000 INFO @ Tue, 02 Aug 2022 15:02:13: 11000000 INFO @ Tue, 02 Aug 2022 15:02:15: 15000000 INFO @ Tue, 02 Aug 2022 15:02:16: 7000000 INFO @ Tue, 02 Aug 2022 15:02:21: 12000000 INFO @ Tue, 02 Aug 2022 15:02:23: 16000000 INFO @ Tue, 02 Aug 2022 15:02:23: 8000000 INFO @ Tue, 02 Aug 2022 15:02:29: 13000000 INFO @ Tue, 02 Aug 2022 15:02:30: 9000000 INFO @ Tue, 02 Aug 2022 15:02:31: 17000000 INFO @ Tue, 02 Aug 2022 15:02:36: 14000000 INFO @ Tue, 02 Aug 2022 15:02:38: 10000000 INFO @ Tue, 02 Aug 2022 15:02:39: 18000000 INFO @ Tue, 02 Aug 2022 15:02:43: 15000000 INFO @ Tue, 02 Aug 2022 15:02:45: 11000000 INFO @ Tue, 02 Aug 2022 15:02:45: #1 tag size is determined as 150 bps INFO @ Tue, 02 Aug 2022 15:02:45: #1 tag size = 150 INFO @ Tue, 02 Aug 2022 15:02:45: #1 total tags in treatment: 18690894 INFO @ Tue, 02 Aug 2022 15:02:45: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 15:02:45: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 15:02:45: #1 tags after filtering in treatment: 18690894 INFO @ Tue, 02 Aug 2022 15:02:45: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 15:02:45: #1 finished! INFO @ Tue, 02 Aug 2022 15:02:45: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 15:02:45: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 15:02:46: #2 number of paired peaks: 88 WARNING @ Tue, 02 Aug 2022 15:02:46: Too few paired peaks (88) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 15:02:46: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX15206449/SRX15206449.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX15206449/SRX15206449.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX15206449/SRX15206449.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX15206449/SRX15206449.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 15:02:51: 16000000 INFO @ Tue, 02 Aug 2022 15:02:52: 12000000 INFO @ Tue, 02 Aug 2022 15:02:58: 17000000 INFO @ Tue, 02 Aug 2022 15:02:59: 13000000 INFO @ Tue, 02 Aug 2022 15:03:05: 18000000 INFO @ Tue, 02 Aug 2022 15:03:06: 14000000 INFO @ Tue, 02 Aug 2022 15:03:10: #1 tag size is determined as 150 bps INFO @ Tue, 02 Aug 2022 15:03:10: #1 tag size = 150 INFO @ Tue, 02 Aug 2022 15:03:10: #1 total tags in treatment: 18690894 INFO @ Tue, 02 Aug 2022 15:03:10: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 15:03:10: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 15:03:11: #1 tags after filtering in treatment: 18690894 INFO @ Tue, 02 Aug 2022 15:03:11: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 15:03:11: #1 finished! INFO @ Tue, 02 Aug 2022 15:03:11: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 15:03:11: #2 looking for paired plus/minus strand peaks... BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 02 Aug 2022 15:03:12: #2 number of paired peaks: 88 WARNING @ Tue, 02 Aug 2022 15:03:12: Too few paired peaks (88) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 15:03:12: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX15206449/SRX15206449.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX15206449/SRX15206449.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX15206449/SRX15206449.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX15206449/SRX15206449.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 15:03:13: 15000000 INFO @ Tue, 02 Aug 2022 15:03:20: 16000000 INFO @ Tue, 02 Aug 2022 15:03:27: 17000000 INFO @ Tue, 02 Aug 2022 15:03:34: 18000000 INFO @ Tue, 02 Aug 2022 15:03:39: #1 tag size is determined as 150 bps INFO @ Tue, 02 Aug 2022 15:03:39: #1 tag size = 150 INFO @ Tue, 02 Aug 2022 15:03:39: #1 total tags in treatment: 18690894 INFO @ Tue, 02 Aug 2022 15:03:39: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 15:03:39: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 15:03:39: #1 tags after filtering in treatment: 18690894 INFO @ Tue, 02 Aug 2022 15:03:39: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 15:03:39: #1 finished! INFO @ Tue, 02 Aug 2022 15:03:39: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 15:03:39: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 15:03:40: #2 number of paired peaks: 88 WARNING @ Tue, 02 Aug 2022 15:03:40: Too few paired peaks (88) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 15:03:40: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX15206449/SRX15206449.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX15206449/SRX15206449.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX15206449/SRX15206449.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX15206449/SRX15206449.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。