Job ID = 16438995 SRX = SRX15206438 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2022-08-02T05:20:52 prefetch.2.10.7: 1) Downloading 'SRR19139373'... 2022-08-02T05:20:52 prefetch.2.10.7: Downloading via HTTPS... 2022-08-02T05:21:44 prefetch.2.10.7: HTTPS download succeed 2022-08-02T05:21:44 prefetch.2.10.7: 1) 'SRR19139373' was downloaded successfully 2022-08-02T05:21:44 prefetch.2.10.7: 'SRR19139373' has 0 unresolved dependencies Read 22813199 spots for SRR19139373/SRR19139373.sra Written 22813199 spots for SRR19139373/SRR19139373.sra fastq に変換しました。 bowtie でマッピング中... Your job 16439166 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:13:36 22813199 reads; of these: 22813199 (100.00%) were unpaired; of these: 508076 (2.23%) aligned 0 times 18757374 (82.22%) aligned exactly 1 time 3547749 (15.55%) aligned >1 times 97.77% overall alignment rate Time searching: 00:13:36 Overall time: 00:13:36 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 1618868 / 22305123 = 0.0726 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 14:47:29: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX15206438/SRX15206438.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX15206438/SRX15206438.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX15206438/SRX15206438.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX15206438/SRX15206438.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 14:47:29: #1 read tag files... INFO @ Tue, 02 Aug 2022 14:47:29: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 14:47:44: 1000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 14:47:58: 2000000 INFO @ Tue, 02 Aug 2022 14:47:59: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX15206438/SRX15206438.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX15206438/SRX15206438.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX15206438/SRX15206438.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX15206438/SRX15206438.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 14:47:59: #1 read tag files... INFO @ Tue, 02 Aug 2022 14:47:59: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 14:48:13: 3000000 INFO @ Tue, 02 Aug 2022 14:48:14: 1000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 14:48:28: 4000000 INFO @ Tue, 02 Aug 2022 14:48:29: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX15206438/SRX15206438.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX15206438/SRX15206438.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX15206438/SRX15206438.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX15206438/SRX15206438.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 14:48:29: #1 read tag files... INFO @ Tue, 02 Aug 2022 14:48:29: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 14:48:29: 2000000 INFO @ Tue, 02 Aug 2022 14:48:44: 5000000 INFO @ Tue, 02 Aug 2022 14:48:45: 1000000 INFO @ Tue, 02 Aug 2022 14:48:45: 3000000 INFO @ Tue, 02 Aug 2022 14:48:59: 6000000 INFO @ Tue, 02 Aug 2022 14:49:00: 2000000 INFO @ Tue, 02 Aug 2022 14:49:00: 4000000 INFO @ Tue, 02 Aug 2022 14:49:14: 7000000 INFO @ Tue, 02 Aug 2022 14:49:15: 5000000 INFO @ Tue, 02 Aug 2022 14:49:16: 3000000 INFO @ Tue, 02 Aug 2022 14:49:29: 8000000 INFO @ Tue, 02 Aug 2022 14:49:31: 4000000 INFO @ Tue, 02 Aug 2022 14:49:31: 6000000 INFO @ Tue, 02 Aug 2022 14:49:43: 9000000 INFO @ Tue, 02 Aug 2022 14:49:46: 7000000 INFO @ Tue, 02 Aug 2022 14:49:46: 5000000 INFO @ Tue, 02 Aug 2022 14:49:58: 10000000 INFO @ Tue, 02 Aug 2022 14:50:01: 8000000 INFO @ Tue, 02 Aug 2022 14:50:02: 6000000 INFO @ Tue, 02 Aug 2022 14:50:13: 11000000 INFO @ Tue, 02 Aug 2022 14:50:16: 9000000 INFO @ Tue, 02 Aug 2022 14:50:17: 7000000 INFO @ Tue, 02 Aug 2022 14:50:27: 12000000 INFO @ Tue, 02 Aug 2022 14:50:31: 10000000 INFO @ Tue, 02 Aug 2022 14:50:33: 8000000 INFO @ Tue, 02 Aug 2022 14:50:42: 13000000 INFO @ Tue, 02 Aug 2022 14:50:45: 11000000 INFO @ Tue, 02 Aug 2022 14:50:47: 9000000 INFO @ Tue, 02 Aug 2022 14:50:56: 14000000 INFO @ Tue, 02 Aug 2022 14:50:59: 12000000 INFO @ Tue, 02 Aug 2022 14:51:01: 10000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 02 Aug 2022 14:51:09: 15000000 INFO @ Tue, 02 Aug 2022 14:51:12: 13000000 INFO @ Tue, 02 Aug 2022 14:51:15: 11000000 INFO @ Tue, 02 Aug 2022 14:51:24: 16000000 INFO @ Tue, 02 Aug 2022 14:51:26: 14000000 INFO @ Tue, 02 Aug 2022 14:51:29: 12000000 INFO @ Tue, 02 Aug 2022 14:51:38: 17000000 INFO @ Tue, 02 Aug 2022 14:51:40: 15000000 INFO @ Tue, 02 Aug 2022 14:51:42: 13000000 INFO @ Tue, 02 Aug 2022 14:51:52: 18000000 INFO @ Tue, 02 Aug 2022 14:51:54: 16000000 INFO @ Tue, 02 Aug 2022 14:51:55: 14000000 INFO @ Tue, 02 Aug 2022 14:52:07: 19000000 INFO @ Tue, 02 Aug 2022 14:52:08: 17000000 INFO @ Tue, 02 Aug 2022 14:52:09: 15000000 BigWig に変換しました。 INFO @ Tue, 02 Aug 2022 14:52:20: 20000000 INFO @ Tue, 02 Aug 2022 14:52:21: 18000000 INFO @ Tue, 02 Aug 2022 14:52:23: 16000000 INFO @ Tue, 02 Aug 2022 14:52:30: #1 tag size is determined as 100 bps INFO @ Tue, 02 Aug 2022 14:52:30: #1 tag size = 100 INFO @ Tue, 02 Aug 2022 14:52:30: #1 total tags in treatment: 20686255 INFO @ Tue, 02 Aug 2022 14:52:30: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 14:52:30: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 14:52:30: #1 tags after filtering in treatment: 20686255 INFO @ Tue, 02 Aug 2022 14:52:30: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 14:52:30: #1 finished! INFO @ Tue, 02 Aug 2022 14:52:30: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 14:52:30: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 14:52:32: #2 number of paired peaks: 80 WARNING @ Tue, 02 Aug 2022 14:52:32: Too few paired peaks (80) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 14:52:32: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX15206438/SRX15206438.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX15206438/SRX15206438.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX15206438/SRX15206438.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX15206438/SRX15206438.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 14:52:34: 19000000 INFO @ Tue, 02 Aug 2022 14:52:37: 17000000 INFO @ Tue, 02 Aug 2022 14:52:46: 20000000 INFO @ Tue, 02 Aug 2022 14:52:50: 18000000 INFO @ Tue, 02 Aug 2022 14:52:54: #1 tag size is determined as 100 bps INFO @ Tue, 02 Aug 2022 14:52:54: #1 tag size = 100 INFO @ Tue, 02 Aug 2022 14:52:54: #1 total tags in treatment: 20686255 INFO @ Tue, 02 Aug 2022 14:52:54: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 14:52:54: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 14:52:55: #1 tags after filtering in treatment: 20686255 INFO @ Tue, 02 Aug 2022 14:52:55: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 14:52:55: #1 finished! INFO @ Tue, 02 Aug 2022 14:52:55: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 14:52:55: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 14:52:57: #2 number of paired peaks: 80 WARNING @ Tue, 02 Aug 2022 14:52:57: Too few paired peaks (80) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 14:52:57: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX15206438/SRX15206438.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX15206438/SRX15206438.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX15206438/SRX15206438.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX15206438/SRX15206438.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 14:53:04: 19000000 INFO @ Tue, 02 Aug 2022 14:53:16: 20000000 INFO @ Tue, 02 Aug 2022 14:53:25: #1 tag size is determined as 100 bps INFO @ Tue, 02 Aug 2022 14:53:25: #1 tag size = 100 INFO @ Tue, 02 Aug 2022 14:53:25: #1 total tags in treatment: 20686255 INFO @ Tue, 02 Aug 2022 14:53:25: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 14:53:25: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 14:53:26: #1 tags after filtering in treatment: 20686255 INFO @ Tue, 02 Aug 2022 14:53:26: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 14:53:26: #1 finished! INFO @ Tue, 02 Aug 2022 14:53:26: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 14:53:26: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 14:53:27: #2 number of paired peaks: 80 WARNING @ Tue, 02 Aug 2022 14:53:27: Too few paired peaks (80) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 14:53:27: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX15206438/SRX15206438.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX15206438/SRX15206438.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX15206438/SRX15206438.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX15206438/SRX15206438.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling