Job ID = 16438994 SRX = SRX15206437 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2022-08-02T05:20:48 prefetch.2.10.7: 1) Downloading 'SRR19139374'... 2022-08-02T05:20:48 prefetch.2.10.7: Downloading via HTTPS... 2022-08-02T05:21:19 prefetch.2.10.7: HTTPS download succeed 2022-08-02T05:21:20 prefetch.2.10.7: 'SRR19139374' is valid 2022-08-02T05:21:20 prefetch.2.10.7: 1) 'SRR19139374' was downloaded successfully 2022-08-02T05:21:20 prefetch.2.10.7: 'SRR19139374' has 0 unresolved dependencies Read 18417852 spots for SRR19139374/SRR19139374.sra Written 18417852 spots for SRR19139374/SRR19139374.sra fastq に変換しました。 bowtie でマッピング中... Your job 16439148 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:15 18417852 reads; of these: 18417852 (100.00%) were unpaired; of these: 366774 (1.99%) aligned 0 times 16200388 (87.96%) aligned exactly 1 time 1850690 (10.05%) aligned >1 times 98.01% overall alignment rate Time searching: 00:06:15 Overall time: 00:06:15 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 1613582 / 18051078 = 0.0894 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 14:35:18: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX15206437/SRX15206437.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX15206437/SRX15206437.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX15206437/SRX15206437.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX15206437/SRX15206437.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 14:35:18: #1 read tag files... INFO @ Tue, 02 Aug 2022 14:35:18: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 14:35:26: 1000000 INFO @ Tue, 02 Aug 2022 14:35:34: 2000000 INFO @ Tue, 02 Aug 2022 14:35:42: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 14:35:47: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX15206437/SRX15206437.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX15206437/SRX15206437.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX15206437/SRX15206437.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX15206437/SRX15206437.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 14:35:47: #1 read tag files... INFO @ Tue, 02 Aug 2022 14:35:47: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 14:35:50: 4000000 INFO @ Tue, 02 Aug 2022 14:35:56: 1000000 INFO @ Tue, 02 Aug 2022 14:35:59: 5000000 INFO @ Tue, 02 Aug 2022 14:36:05: 2000000 INFO @ Tue, 02 Aug 2022 14:36:09: 6000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 14:36:15: 3000000 INFO @ Tue, 02 Aug 2022 14:36:17: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX15206437/SRX15206437.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX15206437/SRX15206437.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX15206437/SRX15206437.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX15206437/SRX15206437.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 14:36:17: #1 read tag files... INFO @ Tue, 02 Aug 2022 14:36:17: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 14:36:18: 7000000 INFO @ Tue, 02 Aug 2022 14:36:25: 4000000 INFO @ Tue, 02 Aug 2022 14:36:25: 1000000 INFO @ Tue, 02 Aug 2022 14:36:28: 8000000 INFO @ Tue, 02 Aug 2022 14:36:33: 2000000 INFO @ Tue, 02 Aug 2022 14:36:35: 5000000 INFO @ Tue, 02 Aug 2022 14:36:38: 9000000 INFO @ Tue, 02 Aug 2022 14:36:40: 3000000 INFO @ Tue, 02 Aug 2022 14:36:45: 6000000 INFO @ Tue, 02 Aug 2022 14:36:47: 4000000 INFO @ Tue, 02 Aug 2022 14:36:48: 10000000 INFO @ Tue, 02 Aug 2022 14:36:54: 5000000 INFO @ Tue, 02 Aug 2022 14:36:54: 7000000 INFO @ Tue, 02 Aug 2022 14:36:58: 11000000 INFO @ Tue, 02 Aug 2022 14:37:02: 6000000 INFO @ Tue, 02 Aug 2022 14:37:04: 8000000 INFO @ Tue, 02 Aug 2022 14:37:08: 12000000 INFO @ Tue, 02 Aug 2022 14:37:09: 7000000 INFO @ Tue, 02 Aug 2022 14:37:14: 9000000 INFO @ Tue, 02 Aug 2022 14:37:16: 8000000 INFO @ Tue, 02 Aug 2022 14:37:18: 13000000 INFO @ Tue, 02 Aug 2022 14:37:23: 9000000 INFO @ Tue, 02 Aug 2022 14:37:24: 10000000 INFO @ Tue, 02 Aug 2022 14:37:28: 14000000 INFO @ Tue, 02 Aug 2022 14:37:30: 10000000 INFO @ Tue, 02 Aug 2022 14:37:33: 11000000 INFO @ Tue, 02 Aug 2022 14:37:37: 11000000 INFO @ Tue, 02 Aug 2022 14:37:38: 15000000 INFO @ Tue, 02 Aug 2022 14:37:43: 12000000 INFO @ Tue, 02 Aug 2022 14:37:44: 12000000 INFO @ Tue, 02 Aug 2022 14:37:47: 16000000 INFO @ Tue, 02 Aug 2022 14:37:51: #1 tag size is determined as 74 bps INFO @ Tue, 02 Aug 2022 14:37:51: #1 tag size = 74 INFO @ Tue, 02 Aug 2022 14:37:51: #1 total tags in treatment: 16437496 INFO @ Tue, 02 Aug 2022 14:37:51: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 14:37:51: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 14:37:51: #1 tags after filtering in treatment: 16437496 INFO @ Tue, 02 Aug 2022 14:37:51: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 14:37:51: #1 finished! INFO @ Tue, 02 Aug 2022 14:37:51: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 14:37:51: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 14:37:52: 13000000 INFO @ Tue, 02 Aug 2022 14:37:52: 13000000 INFO @ Tue, 02 Aug 2022 14:37:52: #2 number of paired peaks: 68 WARNING @ Tue, 02 Aug 2022 14:37:52: Too few paired peaks (68) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 14:37:52: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX15206437/SRX15206437.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX15206437/SRX15206437.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX15206437/SRX15206437.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX15206437/SRX15206437.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 14:37:59: 14000000 INFO @ Tue, 02 Aug 2022 14:38:00: 14000000 INFO @ Tue, 02 Aug 2022 14:38:06: 15000000 INFO @ Tue, 02 Aug 2022 14:38:08: 15000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 02 Aug 2022 14:38:14: 16000000 INFO @ Tue, 02 Aug 2022 14:38:16: 16000000 INFO @ Tue, 02 Aug 2022 14:38:17: #1 tag size is determined as 74 bps INFO @ Tue, 02 Aug 2022 14:38:17: #1 tag size = 74 INFO @ Tue, 02 Aug 2022 14:38:17: #1 total tags in treatment: 16437496 INFO @ Tue, 02 Aug 2022 14:38:17: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 14:38:17: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 14:38:17: #1 tags after filtering in treatment: 16437496 INFO @ Tue, 02 Aug 2022 14:38:17: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 14:38:17: #1 finished! INFO @ Tue, 02 Aug 2022 14:38:17: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 14:38:17: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 14:38:18: #2 number of paired peaks: 68 WARNING @ Tue, 02 Aug 2022 14:38:18: Too few paired peaks (68) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 14:38:18: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX15206437/SRX15206437.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX15206437/SRX15206437.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX15206437/SRX15206437.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX15206437/SRX15206437.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 14:38:19: #1 tag size is determined as 74 bps INFO @ Tue, 02 Aug 2022 14:38:19: #1 tag size = 74 INFO @ Tue, 02 Aug 2022 14:38:19: #1 total tags in treatment: 16437496 INFO @ Tue, 02 Aug 2022 14:38:19: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 14:38:19: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 14:38:20: #1 tags after filtering in treatment: 16437496 INFO @ Tue, 02 Aug 2022 14:38:20: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 14:38:20: #1 finished! INFO @ Tue, 02 Aug 2022 14:38:20: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 14:38:20: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 14:38:21: #2 number of paired peaks: 68 WARNING @ Tue, 02 Aug 2022 14:38:21: Too few paired peaks (68) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 14:38:21: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX15206437/SRX15206437.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX15206437/SRX15206437.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX15206437/SRX15206437.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX15206437/SRX15206437.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。