Job ID = 16437456 SRX = SRX15046051 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 21097451 spots for SRR18970063/SRR18970063.sra Written 21097451 spots for SRR18970063/SRR18970063.sra Read 24763986 spots for SRR18970064/SRR18970064.sra Written 24763986 spots for SRR18970064/SRR18970064.sra fastq に変換しました。 bowtie でマッピング中... Your job 16437608 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:13:48 45861437 reads; of these: 45861437 (100.00%) were unpaired; of these: 2840786 (6.19%) aligned 0 times 35333429 (77.04%) aligned exactly 1 time 7687222 (16.76%) aligned >1 times 93.81% overall alignment rate Time searching: 00:13:48 Overall time: 00:13:48 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 9249464 / 43020651 = 0.2150 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 13:01:26: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX15046051/SRX15046051.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX15046051/SRX15046051.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX15046051/SRX15046051.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX15046051/SRX15046051.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 13:01:26: #1 read tag files... INFO @ Tue, 02 Aug 2022 13:01:26: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 13:01:34: 1000000 INFO @ Tue, 02 Aug 2022 13:01:41: 2000000 INFO @ Tue, 02 Aug 2022 13:01:48: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 13:01:55: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX15046051/SRX15046051.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX15046051/SRX15046051.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX15046051/SRX15046051.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX15046051/SRX15046051.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 13:01:55: #1 read tag files... INFO @ Tue, 02 Aug 2022 13:01:55: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 13:01:55: 4000000 INFO @ Tue, 02 Aug 2022 13:02:02: 1000000 INFO @ Tue, 02 Aug 2022 13:02:02: 5000000 INFO @ Tue, 02 Aug 2022 13:02:09: 2000000 INFO @ Tue, 02 Aug 2022 13:02:10: 6000000 INFO @ Tue, 02 Aug 2022 13:02:15: 3000000 INFO @ Tue, 02 Aug 2022 13:02:17: 7000000 INFO @ Tue, 02 Aug 2022 13:02:21: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 13:02:24: 8000000 INFO @ Tue, 02 Aug 2022 13:02:25: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX15046051/SRX15046051.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX15046051/SRX15046051.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX15046051/SRX15046051.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX15046051/SRX15046051.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 13:02:25: #1 read tag files... INFO @ Tue, 02 Aug 2022 13:02:25: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 13:02:27: 5000000 INFO @ Tue, 02 Aug 2022 13:02:32: 9000000 INFO @ Tue, 02 Aug 2022 13:02:34: 1000000 INFO @ Tue, 02 Aug 2022 13:02:34: 6000000 INFO @ Tue, 02 Aug 2022 13:02:40: 10000000 INFO @ Tue, 02 Aug 2022 13:02:40: 7000000 INFO @ Tue, 02 Aug 2022 13:02:43: 2000000 INFO @ Tue, 02 Aug 2022 13:02:47: 8000000 INFO @ Tue, 02 Aug 2022 13:02:48: 11000000 INFO @ Tue, 02 Aug 2022 13:02:50: 3000000 INFO @ Tue, 02 Aug 2022 13:02:53: 9000000 INFO @ Tue, 02 Aug 2022 13:02:55: 12000000 INFO @ Tue, 02 Aug 2022 13:02:59: 4000000 INFO @ Tue, 02 Aug 2022 13:02:59: 10000000 INFO @ Tue, 02 Aug 2022 13:03:04: 13000000 INFO @ Tue, 02 Aug 2022 13:03:06: 11000000 INFO @ Tue, 02 Aug 2022 13:03:07: 5000000 INFO @ Tue, 02 Aug 2022 13:03:13: 12000000 INFO @ Tue, 02 Aug 2022 13:03:13: 14000000 INFO @ Tue, 02 Aug 2022 13:03:16: 6000000 INFO @ Tue, 02 Aug 2022 13:03:20: 13000000 INFO @ Tue, 02 Aug 2022 13:03:22: 15000000 INFO @ Tue, 02 Aug 2022 13:03:24: 7000000 INFO @ Tue, 02 Aug 2022 13:03:26: 14000000 INFO @ Tue, 02 Aug 2022 13:03:30: 16000000 INFO @ Tue, 02 Aug 2022 13:03:33: 15000000 INFO @ Tue, 02 Aug 2022 13:03:33: 8000000 INFO @ Tue, 02 Aug 2022 13:03:39: 17000000 INFO @ Tue, 02 Aug 2022 13:03:39: 16000000 INFO @ Tue, 02 Aug 2022 13:03:41: 9000000 INFO @ Tue, 02 Aug 2022 13:03:46: 17000000 INFO @ Tue, 02 Aug 2022 13:03:46: 18000000 INFO @ Tue, 02 Aug 2022 13:03:49: 10000000 INFO @ Tue, 02 Aug 2022 13:03:52: 18000000 INFO @ Tue, 02 Aug 2022 13:03:54: 19000000 INFO @ Tue, 02 Aug 2022 13:03:57: 11000000 INFO @ Tue, 02 Aug 2022 13:03:59: 19000000 INFO @ Tue, 02 Aug 2022 13:04:06: 20000000 INFO @ Tue, 02 Aug 2022 13:04:06: 12000000 INFO @ Tue, 02 Aug 2022 13:04:07: 20000000 INFO @ Tue, 02 Aug 2022 13:04:14: 21000000 INFO @ Tue, 02 Aug 2022 13:04:15: 13000000 INFO @ Tue, 02 Aug 2022 13:04:16: 21000000 INFO @ Tue, 02 Aug 2022 13:04:21: 22000000 INFO @ Tue, 02 Aug 2022 13:04:24: 14000000 INFO @ Tue, 02 Aug 2022 13:04:24: 22000000 INFO @ Tue, 02 Aug 2022 13:04:28: 23000000 INFO @ Tue, 02 Aug 2022 13:04:33: 15000000 INFO @ Tue, 02 Aug 2022 13:04:33: 23000000 INFO @ Tue, 02 Aug 2022 13:04:36: 24000000 INFO @ Tue, 02 Aug 2022 13:04:42: 16000000 INFO @ Tue, 02 Aug 2022 13:04:42: 25000000 INFO @ Tue, 02 Aug 2022 13:04:43: 24000000 INFO @ Tue, 02 Aug 2022 13:04:49: 26000000 INFO @ Tue, 02 Aug 2022 13:04:51: 17000000 INFO @ Tue, 02 Aug 2022 13:04:52: 25000000 INFO @ Tue, 02 Aug 2022 13:04:56: 27000000 INFO @ Tue, 02 Aug 2022 13:05:00: 18000000 INFO @ Tue, 02 Aug 2022 13:05:01: 26000000 INFO @ Tue, 02 Aug 2022 13:05:04: 28000000 INFO @ Tue, 02 Aug 2022 13:05:09: 19000000 INFO @ Tue, 02 Aug 2022 13:05:10: 27000000 INFO @ Tue, 02 Aug 2022 13:05:10: 29000000 INFO @ Tue, 02 Aug 2022 13:05:17: 30000000 INFO @ Tue, 02 Aug 2022 13:05:18: 20000000 INFO @ Tue, 02 Aug 2022 13:05:19: 28000000 INFO @ Tue, 02 Aug 2022 13:05:23: 31000000 INFO @ Tue, 02 Aug 2022 13:05:27: 21000000 INFO @ Tue, 02 Aug 2022 13:05:28: 29000000 INFO @ Tue, 02 Aug 2022 13:05:30: 32000000 INFO @ Tue, 02 Aug 2022 13:05:37: 22000000 INFO @ Tue, 02 Aug 2022 13:05:37: 30000000 INFO @ Tue, 02 Aug 2022 13:05:38: 33000000 INFO @ Tue, 02 Aug 2022 13:05:44: #1 tag size is determined as 51 bps INFO @ Tue, 02 Aug 2022 13:05:44: #1 tag size = 51 INFO @ Tue, 02 Aug 2022 13:05:44: #1 total tags in treatment: 33771187 INFO @ Tue, 02 Aug 2022 13:05:44: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 13:05:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 13:05:44: #1 tags after filtering in treatment: 33771187 INFO @ Tue, 02 Aug 2022 13:05:44: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 13:05:44: #1 finished! INFO @ Tue, 02 Aug 2022 13:05:44: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 13:05:44: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 13:05:46: 23000000 INFO @ Tue, 02 Aug 2022 13:05:46: 31000000 INFO @ Tue, 02 Aug 2022 13:05:47: #2 number of paired peaks: 1 WARNING @ Tue, 02 Aug 2022 13:05:47: Too few paired peaks (1) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 13:05:47: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX15046051/SRX15046051.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX15046051/SRX15046051.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX15046051/SRX15046051.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX15046051/SRX15046051.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 13:05:54: 24000000 INFO @ Tue, 02 Aug 2022 13:05:55: 32000000 INFO @ Tue, 02 Aug 2022 13:06:03: 25000000 INFO @ Tue, 02 Aug 2022 13:06:03: 33000000 INFO @ Tue, 02 Aug 2022 13:06:10: #1 tag size is determined as 51 bps INFO @ Tue, 02 Aug 2022 13:06:10: #1 tag size = 51 INFO @ Tue, 02 Aug 2022 13:06:10: #1 total tags in treatment: 33771187 INFO @ Tue, 02 Aug 2022 13:06:10: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 13:06:10: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 13:06:11: #1 tags after filtering in treatment: 33771187 INFO @ Tue, 02 Aug 2022 13:06:11: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 13:06:11: #1 finished! INFO @ Tue, 02 Aug 2022 13:06:11: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 13:06:11: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 13:06:12: 26000000 INFO @ Tue, 02 Aug 2022 13:06:13: #2 number of paired peaks: 1 WARNING @ Tue, 02 Aug 2022 13:06:13: Too few paired peaks (1) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 13:06:13: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX15046051/SRX15046051.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX15046051/SRX15046051.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX15046051/SRX15046051.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX15046051/SRX15046051.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 13:06:20: 27000000 INFO @ Tue, 02 Aug 2022 13:06:28: 28000000 INFO @ Tue, 02 Aug 2022 13:06:35: 29000000 INFO @ Tue, 02 Aug 2022 13:06:43: 30000000 INFO @ Tue, 02 Aug 2022 13:06:51: 31000000 INFO @ Tue, 02 Aug 2022 13:06:58: 32000000 INFO @ Tue, 02 Aug 2022 13:07:06: 33000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 02 Aug 2022 13:07:12: #1 tag size is determined as 51 bps INFO @ Tue, 02 Aug 2022 13:07:12: #1 tag size = 51 INFO @ Tue, 02 Aug 2022 13:07:12: #1 total tags in treatment: 33771187 INFO @ Tue, 02 Aug 2022 13:07:12: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 13:07:12: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 13:07:12: #1 tags after filtering in treatment: 33771187 INFO @ Tue, 02 Aug 2022 13:07:12: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 13:07:12: #1 finished! INFO @ Tue, 02 Aug 2022 13:07:12: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 13:07:12: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 13:07:15: #2 number of paired peaks: 1 WARNING @ Tue, 02 Aug 2022 13:07:15: Too few paired peaks (1) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 13:07:15: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX15046051/SRX15046051.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 7 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX15046051/SRX15046051.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX15046051/SRX15046051.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX15046051/SRX15046051.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。