Job ID = 16439740 SRX = SRX14894231 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2022-08-02T07:03:51 prefetch.2.10.7: 1) Downloading 'SRR18795790'... 2022-08-02T07:03:51 prefetch.2.10.7: Downloading via HTTPS... 2022-08-02T07:05:10 prefetch.2.10.7: HTTPS download succeed 2022-08-02T07:05:10 prefetch.2.10.7: 1) 'SRR18795790' was downloaded successfully 2022-08-02T07:05:10 prefetch.2.10.7: 'SRR18795790' has 0 unresolved dependencies Read 59028466 spots for SRR18795790/SRR18795790.sra Written 59028466 spots for SRR18795790/SRR18795790.sra fastq に変換しました。 bowtie でマッピング中... Your job 16439990 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:01 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:19:36 59028466 reads; of these: 59028466 (100.00%) were unpaired; of these: 6192985 (10.49%) aligned 0 times 38623807 (65.43%) aligned exactly 1 time 14211674 (24.08%) aligned >1 times 89.51% overall alignment rate Time searching: 00:19:37 Overall time: 00:19:37 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 24 files... [bam_rmdupse_core] 13257277 / 52835481 = 0.2509 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 16:37:37: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX14894231/SRX14894231.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX14894231/SRX14894231.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX14894231/SRX14894231.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX14894231/SRX14894231.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 16:37:37: #1 read tag files... INFO @ Tue, 02 Aug 2022 16:37:37: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 16:37:43: 1000000 INFO @ Tue, 02 Aug 2022 16:37:49: 2000000 INFO @ Tue, 02 Aug 2022 16:37:55: 3000000 INFO @ Tue, 02 Aug 2022 16:38:00: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 16:38:06: 5000000 INFO @ Tue, 02 Aug 2022 16:38:07: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX14894231/SRX14894231.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX14894231/SRX14894231.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX14894231/SRX14894231.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX14894231/SRX14894231.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 16:38:07: #1 read tag files... INFO @ Tue, 02 Aug 2022 16:38:07: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 16:38:13: 6000000 INFO @ Tue, 02 Aug 2022 16:38:14: 1000000 INFO @ Tue, 02 Aug 2022 16:38:19: 7000000 INFO @ Tue, 02 Aug 2022 16:38:20: 2000000 INFO @ Tue, 02 Aug 2022 16:38:26: 8000000 INFO @ Tue, 02 Aug 2022 16:38:27: 3000000 INFO @ Tue, 02 Aug 2022 16:38:32: 9000000 INFO @ Tue, 02 Aug 2022 16:38:34: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 16:38:37: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX14894231/SRX14894231.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX14894231/SRX14894231.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX14894231/SRX14894231.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX14894231/SRX14894231.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 16:38:37: #1 read tag files... INFO @ Tue, 02 Aug 2022 16:38:37: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 16:38:39: 10000000 INFO @ Tue, 02 Aug 2022 16:38:41: 5000000 INFO @ Tue, 02 Aug 2022 16:38:44: 1000000 INFO @ Tue, 02 Aug 2022 16:38:46: 11000000 INFO @ Tue, 02 Aug 2022 16:38:48: 6000000 INFO @ Tue, 02 Aug 2022 16:38:52: 2000000 INFO @ Tue, 02 Aug 2022 16:38:53: 12000000 INFO @ Tue, 02 Aug 2022 16:38:54: 7000000 INFO @ Tue, 02 Aug 2022 16:38:59: 3000000 INFO @ Tue, 02 Aug 2022 16:39:00: 13000000 INFO @ Tue, 02 Aug 2022 16:39:01: 8000000 INFO @ Tue, 02 Aug 2022 16:39:07: 14000000 INFO @ Tue, 02 Aug 2022 16:39:07: 4000000 INFO @ Tue, 02 Aug 2022 16:39:08: 9000000 INFO @ Tue, 02 Aug 2022 16:39:14: 15000000 INFO @ Tue, 02 Aug 2022 16:39:14: 5000000 INFO @ Tue, 02 Aug 2022 16:39:15: 10000000 INFO @ Tue, 02 Aug 2022 16:39:21: 16000000 INFO @ Tue, 02 Aug 2022 16:39:21: 6000000 INFO @ Tue, 02 Aug 2022 16:39:22: 11000000 INFO @ Tue, 02 Aug 2022 16:39:27: 17000000 INFO @ Tue, 02 Aug 2022 16:39:28: 7000000 INFO @ Tue, 02 Aug 2022 16:39:29: 12000000 INFO @ Tue, 02 Aug 2022 16:39:34: 18000000 INFO @ Tue, 02 Aug 2022 16:39:35: 8000000 INFO @ Tue, 02 Aug 2022 16:39:36: 13000000 INFO @ Tue, 02 Aug 2022 16:39:41: 19000000 INFO @ Tue, 02 Aug 2022 16:39:42: 14000000 INFO @ Tue, 02 Aug 2022 16:39:42: 9000000 INFO @ Tue, 02 Aug 2022 16:39:47: 20000000 INFO @ Tue, 02 Aug 2022 16:39:49: 15000000 INFO @ Tue, 02 Aug 2022 16:39:49: 10000000 INFO @ Tue, 02 Aug 2022 16:39:54: 21000000 INFO @ Tue, 02 Aug 2022 16:39:55: 16000000 INFO @ Tue, 02 Aug 2022 16:39:56: 11000000 INFO @ Tue, 02 Aug 2022 16:40:01: 22000000 INFO @ Tue, 02 Aug 2022 16:40:02: 17000000 INFO @ Tue, 02 Aug 2022 16:40:03: 12000000 INFO @ Tue, 02 Aug 2022 16:40:07: 23000000 INFO @ Tue, 02 Aug 2022 16:40:09: 18000000 INFO @ Tue, 02 Aug 2022 16:40:10: 13000000 INFO @ Tue, 02 Aug 2022 16:40:14: 24000000 INFO @ Tue, 02 Aug 2022 16:40:15: 19000000 INFO @ Tue, 02 Aug 2022 16:40:16: 14000000 INFO @ Tue, 02 Aug 2022 16:40:20: 25000000 INFO @ Tue, 02 Aug 2022 16:40:22: 20000000 INFO @ Tue, 02 Aug 2022 16:40:23: 15000000 INFO @ Tue, 02 Aug 2022 16:40:27: 26000000 INFO @ Tue, 02 Aug 2022 16:40:28: 21000000 INFO @ Tue, 02 Aug 2022 16:40:29: 16000000 INFO @ Tue, 02 Aug 2022 16:40:33: 27000000 INFO @ Tue, 02 Aug 2022 16:40:35: 22000000 INFO @ Tue, 02 Aug 2022 16:40:36: 17000000 INFO @ Tue, 02 Aug 2022 16:40:40: 28000000 INFO @ Tue, 02 Aug 2022 16:40:41: 23000000 INFO @ Tue, 02 Aug 2022 16:40:42: 18000000 INFO @ Tue, 02 Aug 2022 16:40:46: 29000000 INFO @ Tue, 02 Aug 2022 16:40:48: 24000000 INFO @ Tue, 02 Aug 2022 16:40:49: 19000000 INFO @ Tue, 02 Aug 2022 16:40:53: 30000000 INFO @ Tue, 02 Aug 2022 16:40:55: 25000000 INFO @ Tue, 02 Aug 2022 16:40:55: 20000000 INFO @ Tue, 02 Aug 2022 16:41:00: 31000000 INFO @ Tue, 02 Aug 2022 16:41:01: 26000000 INFO @ Tue, 02 Aug 2022 16:41:02: 21000000 INFO @ Tue, 02 Aug 2022 16:41:06: 32000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 02 Aug 2022 16:41:07: 27000000 INFO @ Tue, 02 Aug 2022 16:41:08: 22000000 INFO @ Tue, 02 Aug 2022 16:41:13: 33000000 INFO @ Tue, 02 Aug 2022 16:41:14: 28000000 INFO @ Tue, 02 Aug 2022 16:41:15: 23000000 INFO @ Tue, 02 Aug 2022 16:41:19: 34000000 INFO @ Tue, 02 Aug 2022 16:41:21: 29000000 INFO @ Tue, 02 Aug 2022 16:41:21: 24000000 INFO @ Tue, 02 Aug 2022 16:41:26: 35000000 INFO @ Tue, 02 Aug 2022 16:41:27: 30000000 INFO @ Tue, 02 Aug 2022 16:41:28: 25000000 INFO @ Tue, 02 Aug 2022 16:41:32: 36000000 INFO @ Tue, 02 Aug 2022 16:41:34: 31000000 INFO @ Tue, 02 Aug 2022 16:41:34: 26000000 INFO @ Tue, 02 Aug 2022 16:41:39: 37000000 INFO @ Tue, 02 Aug 2022 16:41:40: 32000000 INFO @ Tue, 02 Aug 2022 16:41:40: 27000000 INFO @ Tue, 02 Aug 2022 16:41:45: 38000000 INFO @ Tue, 02 Aug 2022 16:41:47: 33000000 INFO @ Tue, 02 Aug 2022 16:41:47: 28000000 INFO @ Tue, 02 Aug 2022 16:41:51: 39000000 INFO @ Tue, 02 Aug 2022 16:41:53: 34000000 INFO @ Tue, 02 Aug 2022 16:41:53: 29000000 INFO @ Tue, 02 Aug 2022 16:41:55: #1 tag size is determined as 51 bps INFO @ Tue, 02 Aug 2022 16:41:55: #1 tag size = 51 INFO @ Tue, 02 Aug 2022 16:41:55: #1 total tags in treatment: 39578204 INFO @ Tue, 02 Aug 2022 16:41:55: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 16:41:55: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 16:41:56: #1 tags after filtering in treatment: 39578204 INFO @ Tue, 02 Aug 2022 16:41:56: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 16:41:56: #1 finished! INFO @ Tue, 02 Aug 2022 16:41:56: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 16:41:56: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 16:41:58: #2 number of paired peaks: 5 WARNING @ Tue, 02 Aug 2022 16:41:58: Too few paired peaks (5) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 16:41:58: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX14894231/SRX14894231.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX14894231/SRX14894231.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX14894231/SRX14894231.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX14894231/SRX14894231.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 16:41:59: 35000000 INFO @ Tue, 02 Aug 2022 16:42:00: 30000000 INFO @ Tue, 02 Aug 2022 16:42:06: 36000000 INFO @ Tue, 02 Aug 2022 16:42:06: 31000000 INFO @ Tue, 02 Aug 2022 16:42:12: 37000000 INFO @ Tue, 02 Aug 2022 16:42:13: 32000000 INFO @ Tue, 02 Aug 2022 16:42:18: 38000000 BigWig に変換しました。 INFO @ Tue, 02 Aug 2022 16:42:19: 33000000 INFO @ Tue, 02 Aug 2022 16:42:25: 39000000 INFO @ Tue, 02 Aug 2022 16:42:25: 34000000 INFO @ Tue, 02 Aug 2022 16:42:28: #1 tag size is determined as 51 bps INFO @ Tue, 02 Aug 2022 16:42:28: #1 tag size = 51 INFO @ Tue, 02 Aug 2022 16:42:28: #1 total tags in treatment: 39578204 INFO @ Tue, 02 Aug 2022 16:42:28: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 16:42:28: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 16:42:29: #1 tags after filtering in treatment: 39578204 INFO @ Tue, 02 Aug 2022 16:42:29: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 16:42:29: #1 finished! INFO @ Tue, 02 Aug 2022 16:42:29: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 16:42:29: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 16:42:31: 35000000 INFO @ Tue, 02 Aug 2022 16:42:31: #2 number of paired peaks: 5 WARNING @ Tue, 02 Aug 2022 16:42:31: Too few paired peaks (5) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 16:42:31: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX14894231/SRX14894231.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX14894231/SRX14894231.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX14894231/SRX14894231.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX14894231/SRX14894231.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 16:42:37: 36000000 INFO @ Tue, 02 Aug 2022 16:42:42: 37000000 INFO @ Tue, 02 Aug 2022 16:42:48: 38000000 INFO @ Tue, 02 Aug 2022 16:42:54: 39000000 INFO @ Tue, 02 Aug 2022 16:42:57: #1 tag size is determined as 51 bps INFO @ Tue, 02 Aug 2022 16:42:57: #1 tag size = 51 INFO @ Tue, 02 Aug 2022 16:42:57: #1 total tags in treatment: 39578204 INFO @ Tue, 02 Aug 2022 16:42:57: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 16:42:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 16:42:58: #1 tags after filtering in treatment: 39578204 INFO @ Tue, 02 Aug 2022 16:42:58: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 16:42:58: #1 finished! INFO @ Tue, 02 Aug 2022 16:42:58: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 16:42:58: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 16:43:00: #2 number of paired peaks: 5 WARNING @ Tue, 02 Aug 2022 16:43:00: Too few paired peaks (5) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 16:43:00: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX14894231/SRX14894231.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX14894231/SRX14894231.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX14894231/SRX14894231.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX14894231/SRX14894231.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling