Job ID = 16439736 SRX = SRX14894227 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2022-08-02T07:03:06 prefetch.2.10.7: 1) Downloading 'SRR18795794'... 2022-08-02T07:03:06 prefetch.2.10.7: Downloading via HTTPS... 2022-08-02T07:05:00 prefetch.2.10.7: HTTPS download succeed 2022-08-02T07:05:00 prefetch.2.10.7: 1) 'SRR18795794' was downloaded successfully 2022-08-02T07:05:00 prefetch.2.10.7: 'SRR18795794' has 0 unresolved dependencies Read 62569314 spots for SRR18795794/SRR18795794.sra Written 62569314 spots for SRR18795794/SRR18795794.sra fastq に変換しました。 bowtie でマッピング中... Your job 16440004 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:22:25 62569314 reads; of these: 62569314 (100.00%) were unpaired; of these: 5997427 (9.59%) aligned 0 times 40464361 (64.67%) aligned exactly 1 time 16107526 (25.74%) aligned >1 times 90.41% overall alignment rate Time searching: 00:22:25 Overall time: 00:22:25 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 24 files... [bam_rmdupse_core] 21464836 / 56571887 = 0.3794 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 16:46:06: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX14894227/SRX14894227.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX14894227/SRX14894227.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX14894227/SRX14894227.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX14894227/SRX14894227.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 16:46:06: #1 read tag files... INFO @ Tue, 02 Aug 2022 16:46:06: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 16:46:14: 1000000 INFO @ Tue, 02 Aug 2022 16:46:22: 2000000 INFO @ Tue, 02 Aug 2022 16:46:29: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 16:46:35: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX14894227/SRX14894227.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX14894227/SRX14894227.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX14894227/SRX14894227.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX14894227/SRX14894227.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 16:46:35: #1 read tag files... INFO @ Tue, 02 Aug 2022 16:46:35: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 16:46:37: 4000000 INFO @ Tue, 02 Aug 2022 16:46:43: 1000000 INFO @ Tue, 02 Aug 2022 16:46:44: 5000000 INFO @ Tue, 02 Aug 2022 16:46:50: 2000000 INFO @ Tue, 02 Aug 2022 16:46:52: 6000000 INFO @ Tue, 02 Aug 2022 16:46:58: 3000000 INFO @ Tue, 02 Aug 2022 16:46:59: 7000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 16:47:05: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX14894227/SRX14894227.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX14894227/SRX14894227.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX14894227/SRX14894227.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX14894227/SRX14894227.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 16:47:05: #1 read tag files... INFO @ Tue, 02 Aug 2022 16:47:05: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 16:47:05: 4000000 INFO @ Tue, 02 Aug 2022 16:47:07: 8000000 INFO @ Tue, 02 Aug 2022 16:47:13: 5000000 INFO @ Tue, 02 Aug 2022 16:47:13: 1000000 INFO @ Tue, 02 Aug 2022 16:47:14: 9000000 INFO @ Tue, 02 Aug 2022 16:47:20: 6000000 INFO @ Tue, 02 Aug 2022 16:47:21: 2000000 INFO @ Tue, 02 Aug 2022 16:47:22: 10000000 INFO @ Tue, 02 Aug 2022 16:47:28: 7000000 INFO @ Tue, 02 Aug 2022 16:47:29: 3000000 INFO @ Tue, 02 Aug 2022 16:47:29: 11000000 INFO @ Tue, 02 Aug 2022 16:47:36: 8000000 INFO @ Tue, 02 Aug 2022 16:47:37: 12000000 INFO @ Tue, 02 Aug 2022 16:47:37: 4000000 INFO @ Tue, 02 Aug 2022 16:47:43: 9000000 INFO @ Tue, 02 Aug 2022 16:47:45: 13000000 INFO @ Tue, 02 Aug 2022 16:47:45: 5000000 INFO @ Tue, 02 Aug 2022 16:47:51: 10000000 INFO @ Tue, 02 Aug 2022 16:47:52: 14000000 INFO @ Tue, 02 Aug 2022 16:47:54: 6000000 INFO @ Tue, 02 Aug 2022 16:47:58: 11000000 INFO @ Tue, 02 Aug 2022 16:48:00: 15000000 INFO @ Tue, 02 Aug 2022 16:48:02: 7000000 INFO @ Tue, 02 Aug 2022 16:48:06: 12000000 INFO @ Tue, 02 Aug 2022 16:48:07: 16000000 INFO @ Tue, 02 Aug 2022 16:48:11: 8000000 INFO @ Tue, 02 Aug 2022 16:48:13: 13000000 INFO @ Tue, 02 Aug 2022 16:48:15: 17000000 INFO @ Tue, 02 Aug 2022 16:48:19: 9000000 INFO @ Tue, 02 Aug 2022 16:48:21: 14000000 INFO @ Tue, 02 Aug 2022 16:48:23: 18000000 INFO @ Tue, 02 Aug 2022 16:48:26: 10000000 INFO @ Tue, 02 Aug 2022 16:48:29: 15000000 INFO @ Tue, 02 Aug 2022 16:48:31: 19000000 INFO @ Tue, 02 Aug 2022 16:48:35: 11000000 INFO @ Tue, 02 Aug 2022 16:48:36: 16000000 INFO @ Tue, 02 Aug 2022 16:48:38: 20000000 INFO @ Tue, 02 Aug 2022 16:48:43: 12000000 INFO @ Tue, 02 Aug 2022 16:48:44: 17000000 INFO @ Tue, 02 Aug 2022 16:48:46: 21000000 INFO @ Tue, 02 Aug 2022 16:48:51: 13000000 INFO @ Tue, 02 Aug 2022 16:48:51: 18000000 INFO @ Tue, 02 Aug 2022 16:48:53: 22000000 INFO @ Tue, 02 Aug 2022 16:48:59: 19000000 INFO @ Tue, 02 Aug 2022 16:48:59: 14000000 INFO @ Tue, 02 Aug 2022 16:49:01: 23000000 INFO @ Tue, 02 Aug 2022 16:49:06: 20000000 INFO @ Tue, 02 Aug 2022 16:49:07: 15000000 INFO @ Tue, 02 Aug 2022 16:49:08: 24000000 INFO @ Tue, 02 Aug 2022 16:49:13: 21000000 INFO @ Tue, 02 Aug 2022 16:49:15: 16000000 INFO @ Tue, 02 Aug 2022 16:49:16: 25000000 INFO @ Tue, 02 Aug 2022 16:49:21: 22000000 INFO @ Tue, 02 Aug 2022 16:49:23: 17000000 INFO @ Tue, 02 Aug 2022 16:49:23: 26000000 INFO @ Tue, 02 Aug 2022 16:49:28: 23000000 INFO @ Tue, 02 Aug 2022 16:49:31: 27000000 INFO @ Tue, 02 Aug 2022 16:49:31: 18000000 INFO @ Tue, 02 Aug 2022 16:49:36: 24000000 INFO @ Tue, 02 Aug 2022 16:49:39: 28000000 INFO @ Tue, 02 Aug 2022 16:49:39: 19000000 INFO @ Tue, 02 Aug 2022 16:49:44: 25000000 INFO @ Tue, 02 Aug 2022 16:49:47: 29000000 INFO @ Tue, 02 Aug 2022 16:49:47: 20000000 INFO @ Tue, 02 Aug 2022 16:49:51: 26000000 INFO @ Tue, 02 Aug 2022 16:49:55: 21000000 INFO @ Tue, 02 Aug 2022 16:49:55: 30000000 INFO @ Tue, 02 Aug 2022 16:49:59: 27000000 INFO @ Tue, 02 Aug 2022 16:50:03: 22000000 INFO @ Tue, 02 Aug 2022 16:50:03: 31000000 INFO @ Tue, 02 Aug 2022 16:50:07: 28000000 INFO @ Tue, 02 Aug 2022 16:50:10: 32000000 INFO @ Tue, 02 Aug 2022 16:50:11: 23000000 INFO @ Tue, 02 Aug 2022 16:50:14: 29000000 INFO @ Tue, 02 Aug 2022 16:50:18: 33000000 INFO @ Tue, 02 Aug 2022 16:50:19: 24000000 INFO @ Tue, 02 Aug 2022 16:50:22: 30000000 INFO @ Tue, 02 Aug 2022 16:50:26: 34000000 INFO @ Tue, 02 Aug 2022 16:50:26: 25000000 INFO @ Tue, 02 Aug 2022 16:50:30: 31000000 INFO @ Tue, 02 Aug 2022 16:50:33: 35000000 INFO @ Tue, 02 Aug 2022 16:50:35: #1 tag size is determined as 51 bps INFO @ Tue, 02 Aug 2022 16:50:35: #1 tag size = 51 INFO @ Tue, 02 Aug 2022 16:50:35: #1 total tags in treatment: 35107051 INFO @ Tue, 02 Aug 2022 16:50:35: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 16:50:35: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 16:50:35: 26000000 INFO @ Tue, 02 Aug 2022 16:50:35: #1 tags after filtering in treatment: 35107051 INFO @ Tue, 02 Aug 2022 16:50:35: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 16:50:35: #1 finished! INFO @ Tue, 02 Aug 2022 16:50:35: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 16:50:35: #2 looking for paired plus/minus strand peaks... BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 02 Aug 2022 16:50:37: 32000000 INFO @ Tue, 02 Aug 2022 16:50:37: #2 number of paired peaks: 77 WARNING @ Tue, 02 Aug 2022 16:50:37: Too few paired peaks (77) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 16:50:37: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX14894227/SRX14894227.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX14894227/SRX14894227.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX14894227/SRX14894227.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX14894227/SRX14894227.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 16:50:43: 27000000 INFO @ Tue, 02 Aug 2022 16:50:44: 33000000 INFO @ Tue, 02 Aug 2022 16:50:51: 28000000 INFO @ Tue, 02 Aug 2022 16:50:51: 34000000 INFO @ Tue, 02 Aug 2022 16:50:59: 35000000 INFO @ Tue, 02 Aug 2022 16:50:59: 29000000 INFO @ Tue, 02 Aug 2022 16:51:00: #1 tag size is determined as 51 bps INFO @ Tue, 02 Aug 2022 16:51:00: #1 tag size = 51 INFO @ Tue, 02 Aug 2022 16:51:00: #1 total tags in treatment: 35107051 INFO @ Tue, 02 Aug 2022 16:51:00: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 16:51:00: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 16:51:01: #1 tags after filtering in treatment: 35107051 INFO @ Tue, 02 Aug 2022 16:51:01: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 16:51:01: #1 finished! INFO @ Tue, 02 Aug 2022 16:51:01: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 16:51:01: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 16:51:03: #2 number of paired peaks: 77 WARNING @ Tue, 02 Aug 2022 16:51:03: Too few paired peaks (77) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 16:51:03: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX14894227/SRX14894227.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX14894227/SRX14894227.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX14894227/SRX14894227.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX14894227/SRX14894227.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 16:51:07: 30000000 INFO @ Tue, 02 Aug 2022 16:51:14: 31000000 INFO @ Tue, 02 Aug 2022 16:51:22: 32000000 INFO @ Tue, 02 Aug 2022 16:51:29: 33000000 INFO @ Tue, 02 Aug 2022 16:51:37: 34000000 INFO @ Tue, 02 Aug 2022 16:51:45: 35000000 INFO @ Tue, 02 Aug 2022 16:51:46: #1 tag size is determined as 51 bps INFO @ Tue, 02 Aug 2022 16:51:46: #1 tag size = 51 INFO @ Tue, 02 Aug 2022 16:51:46: #1 total tags in treatment: 35107051 INFO @ Tue, 02 Aug 2022 16:51:46: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 16:51:46: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 16:51:47: #1 tags after filtering in treatment: 35107051 INFO @ Tue, 02 Aug 2022 16:51:47: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 16:51:47: #1 finished! INFO @ Tue, 02 Aug 2022 16:51:47: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 16:51:47: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 16:51:49: #2 number of paired peaks: 77 WARNING @ Tue, 02 Aug 2022 16:51:49: Too few paired peaks (77) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 16:51:49: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX14894227/SRX14894227.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX14894227/SRX14894227.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX14894227/SRX14894227.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX14894227/SRX14894227.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。