Job ID = 16439733 SRX = SRX14894226 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2022-08-02T07:02:21 prefetch.2.10.7: 1) Downloading 'SRR18795795'... 2022-08-02T07:02:21 prefetch.2.10.7: Downloading via HTTPS... 2022-08-02T07:04:14 prefetch.2.10.7: HTTPS download succeed 2022-08-02T07:04:14 prefetch.2.10.7: 1) 'SRR18795795' was downloaded successfully 2022-08-02T07:04:14 prefetch.2.10.7: 'SRR18795795' has 0 unresolved dependencies Read 63572032 spots for SRR18795795/SRR18795795.sra Written 63572032 spots for SRR18795795/SRR18795795.sra fastq に変換しました。 bowtie でマッピング中... Your job 16439998 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:20:47 63572032 reads; of these: 63572032 (100.00%) were unpaired; of these: 5974520 (9.40%) aligned 0 times 41622980 (65.47%) aligned exactly 1 time 15974532 (25.13%) aligned >1 times 90.60% overall alignment rate Time searching: 00:20:47 Overall time: 00:20:47 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 24 files... [bam_rmdupse_core] 20268339 / 57597512 = 0.3519 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 16:45:08: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX14894226/SRX14894226.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX14894226/SRX14894226.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX14894226/SRX14894226.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX14894226/SRX14894226.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 16:45:08: #1 read tag files... INFO @ Tue, 02 Aug 2022 16:45:08: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 16:45:16: 1000000 INFO @ Tue, 02 Aug 2022 16:45:24: 2000000 INFO @ Tue, 02 Aug 2022 16:45:32: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 16:45:38: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX14894226/SRX14894226.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX14894226/SRX14894226.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX14894226/SRX14894226.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX14894226/SRX14894226.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 16:45:38: #1 read tag files... INFO @ Tue, 02 Aug 2022 16:45:38: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 16:45:41: 4000000 INFO @ Tue, 02 Aug 2022 16:45:46: 1000000 INFO @ Tue, 02 Aug 2022 16:45:49: 5000000 INFO @ Tue, 02 Aug 2022 16:45:54: 2000000 INFO @ Tue, 02 Aug 2022 16:45:57: 6000000 INFO @ Tue, 02 Aug 2022 16:46:03: 3000000 INFO @ Tue, 02 Aug 2022 16:46:05: 7000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 16:46:08: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX14894226/SRX14894226.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX14894226/SRX14894226.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX14894226/SRX14894226.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX14894226/SRX14894226.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 16:46:08: #1 read tag files... INFO @ Tue, 02 Aug 2022 16:46:08: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 16:46:11: 4000000 INFO @ Tue, 02 Aug 2022 16:46:14: 8000000 INFO @ Tue, 02 Aug 2022 16:46:16: 1000000 INFO @ Tue, 02 Aug 2022 16:46:20: 5000000 INFO @ Tue, 02 Aug 2022 16:46:22: 9000000 INFO @ Tue, 02 Aug 2022 16:46:25: 2000000 INFO @ Tue, 02 Aug 2022 16:46:28: 6000000 INFO @ Tue, 02 Aug 2022 16:46:30: 10000000 INFO @ Tue, 02 Aug 2022 16:46:33: 3000000 INFO @ Tue, 02 Aug 2022 16:46:36: 7000000 INFO @ Tue, 02 Aug 2022 16:46:39: 11000000 INFO @ Tue, 02 Aug 2022 16:46:41: 4000000 INFO @ Tue, 02 Aug 2022 16:46:44: 8000000 INFO @ Tue, 02 Aug 2022 16:46:47: 12000000 INFO @ Tue, 02 Aug 2022 16:46:49: 5000000 INFO @ Tue, 02 Aug 2022 16:46:53: 9000000 INFO @ Tue, 02 Aug 2022 16:46:56: 13000000 INFO @ Tue, 02 Aug 2022 16:46:58: 6000000 INFO @ Tue, 02 Aug 2022 16:47:01: 10000000 INFO @ Tue, 02 Aug 2022 16:47:05: 14000000 INFO @ Tue, 02 Aug 2022 16:47:06: 7000000 INFO @ Tue, 02 Aug 2022 16:47:08: 11000000 INFO @ Tue, 02 Aug 2022 16:47:13: 15000000 INFO @ Tue, 02 Aug 2022 16:47:14: 8000000 INFO @ Tue, 02 Aug 2022 16:47:16: 12000000 INFO @ Tue, 02 Aug 2022 16:47:21: 16000000 INFO @ Tue, 02 Aug 2022 16:47:22: 9000000 INFO @ Tue, 02 Aug 2022 16:47:24: 13000000 INFO @ Tue, 02 Aug 2022 16:47:29: 17000000 INFO @ Tue, 02 Aug 2022 16:47:29: 10000000 INFO @ Tue, 02 Aug 2022 16:47:32: 14000000 INFO @ Tue, 02 Aug 2022 16:47:37: 18000000 INFO @ Tue, 02 Aug 2022 16:47:37: 11000000 INFO @ Tue, 02 Aug 2022 16:47:40: 15000000 INFO @ Tue, 02 Aug 2022 16:47:45: 19000000 INFO @ Tue, 02 Aug 2022 16:47:45: 12000000 INFO @ Tue, 02 Aug 2022 16:47:48: 16000000 INFO @ Tue, 02 Aug 2022 16:47:53: 13000000 INFO @ Tue, 02 Aug 2022 16:47:53: 20000000 INFO @ Tue, 02 Aug 2022 16:47:55: 17000000 INFO @ Tue, 02 Aug 2022 16:48:01: 14000000 INFO @ Tue, 02 Aug 2022 16:48:01: 21000000 INFO @ Tue, 02 Aug 2022 16:48:03: 18000000 INFO @ Tue, 02 Aug 2022 16:48:08: 15000000 INFO @ Tue, 02 Aug 2022 16:48:09: 22000000 INFO @ Tue, 02 Aug 2022 16:48:11: 19000000 INFO @ Tue, 02 Aug 2022 16:48:16: 16000000 INFO @ Tue, 02 Aug 2022 16:48:16: 23000000 INFO @ Tue, 02 Aug 2022 16:48:19: 20000000 INFO @ Tue, 02 Aug 2022 16:48:24: 24000000 INFO @ Tue, 02 Aug 2022 16:48:24: 17000000 INFO @ Tue, 02 Aug 2022 16:48:27: 21000000 INFO @ Tue, 02 Aug 2022 16:48:32: 25000000 INFO @ Tue, 02 Aug 2022 16:48:32: 18000000 INFO @ Tue, 02 Aug 2022 16:48:35: 22000000 INFO @ Tue, 02 Aug 2022 16:48:39: 26000000 INFO @ Tue, 02 Aug 2022 16:48:39: 19000000 INFO @ Tue, 02 Aug 2022 16:48:42: 23000000 INFO @ Tue, 02 Aug 2022 16:48:47: 27000000 INFO @ Tue, 02 Aug 2022 16:48:47: 20000000 INFO @ Tue, 02 Aug 2022 16:48:50: 24000000 INFO @ Tue, 02 Aug 2022 16:48:55: 21000000 INFO @ Tue, 02 Aug 2022 16:48:55: 28000000 INFO @ Tue, 02 Aug 2022 16:48:57: 25000000 INFO @ Tue, 02 Aug 2022 16:49:02: 22000000 INFO @ Tue, 02 Aug 2022 16:49:02: 29000000 INFO @ Tue, 02 Aug 2022 16:49:05: 26000000 INFO @ Tue, 02 Aug 2022 16:49:10: 23000000 INFO @ Tue, 02 Aug 2022 16:49:10: 30000000 INFO @ Tue, 02 Aug 2022 16:49:13: 27000000 INFO @ Tue, 02 Aug 2022 16:49:17: 24000000 INFO @ Tue, 02 Aug 2022 16:49:18: 31000000 INFO @ Tue, 02 Aug 2022 16:49:20: 28000000 INFO @ Tue, 02 Aug 2022 16:49:25: 25000000 INFO @ Tue, 02 Aug 2022 16:49:26: 32000000 INFO @ Tue, 02 Aug 2022 16:49:28: 29000000 INFO @ Tue, 02 Aug 2022 16:49:32: 26000000 INFO @ Tue, 02 Aug 2022 16:49:34: 33000000 INFO @ Tue, 02 Aug 2022 16:49:35: 30000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 02 Aug 2022 16:49:40: 27000000 INFO @ Tue, 02 Aug 2022 16:49:41: 34000000 INFO @ Tue, 02 Aug 2022 16:49:43: 31000000 INFO @ Tue, 02 Aug 2022 16:49:47: 28000000 INFO @ Tue, 02 Aug 2022 16:49:49: 35000000 INFO @ Tue, 02 Aug 2022 16:49:50: 32000000 INFO @ Tue, 02 Aug 2022 16:49:55: 29000000 INFO @ Tue, 02 Aug 2022 16:49:56: 36000000 INFO @ Tue, 02 Aug 2022 16:49:58: 33000000 INFO @ Tue, 02 Aug 2022 16:50:02: 30000000 INFO @ Tue, 02 Aug 2022 16:50:04: 37000000 INFO @ Tue, 02 Aug 2022 16:50:05: 34000000 INFO @ Tue, 02 Aug 2022 16:50:07: #1 tag size is determined as 51 bps INFO @ Tue, 02 Aug 2022 16:50:07: #1 tag size = 51 INFO @ Tue, 02 Aug 2022 16:50:07: #1 total tags in treatment: 37329173 INFO @ Tue, 02 Aug 2022 16:50:07: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 16:50:07: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 16:50:07: #1 tags after filtering in treatment: 37329173 INFO @ Tue, 02 Aug 2022 16:50:07: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 16:50:07: #1 finished! INFO @ Tue, 02 Aug 2022 16:50:07: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 16:50:07: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 16:50:10: #2 number of paired peaks: 69 WARNING @ Tue, 02 Aug 2022 16:50:10: Too few paired peaks (69) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 16:50:10: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX14894226/SRX14894226.05_peaks.narrowPeak: No such file or directory INFO @ Tue, 02 Aug 2022 16:50:10: 31000000 pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX14894226/SRX14894226.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX14894226/SRX14894226.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX14894226/SRX14894226.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 16:50:13: 35000000 INFO @ Tue, 02 Aug 2022 16:50:17: 32000000 INFO @ Tue, 02 Aug 2022 16:50:20: 36000000 INFO @ Tue, 02 Aug 2022 16:50:24: 33000000 INFO @ Tue, 02 Aug 2022 16:50:27: 37000000 INFO @ Tue, 02 Aug 2022 16:50:30: #1 tag size is determined as 51 bps INFO @ Tue, 02 Aug 2022 16:50:30: #1 tag size = 51 INFO @ Tue, 02 Aug 2022 16:50:30: #1 total tags in treatment: 37329173 INFO @ Tue, 02 Aug 2022 16:50:30: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 16:50:30: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 16:50:31: #1 tags after filtering in treatment: 37329173 INFO @ Tue, 02 Aug 2022 16:50:31: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 16:50:31: #1 finished! INFO @ Tue, 02 Aug 2022 16:50:31: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 16:50:31: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 16:50:32: 34000000 INFO @ Tue, 02 Aug 2022 16:50:33: #2 number of paired peaks: 69 WARNING @ Tue, 02 Aug 2022 16:50:33: Too few paired peaks (69) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 16:50:33: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX14894226/SRX14894226.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX14894226/SRX14894226.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX14894226/SRX14894226.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX14894226/SRX14894226.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 16:50:39: 35000000 INFO @ Tue, 02 Aug 2022 16:50:46: 36000000 INFO @ Tue, 02 Aug 2022 16:50:53: 37000000 INFO @ Tue, 02 Aug 2022 16:50:55: #1 tag size is determined as 51 bps INFO @ Tue, 02 Aug 2022 16:50:55: #1 tag size = 51 INFO @ Tue, 02 Aug 2022 16:50:55: #1 total tags in treatment: 37329173 INFO @ Tue, 02 Aug 2022 16:50:55: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 16:50:55: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 16:50:56: #1 tags after filtering in treatment: 37329173 INFO @ Tue, 02 Aug 2022 16:50:56: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 16:50:56: #1 finished! INFO @ Tue, 02 Aug 2022 16:50:56: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 16:50:56: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 16:50:59: #2 number of paired peaks: 69 WARNING @ Tue, 02 Aug 2022 16:50:59: Too few paired peaks (69) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 16:50:59: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX14894226/SRX14894226.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX14894226/SRX14894226.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX14894226/SRX14894226.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX14894226/SRX14894226.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。