Job ID = 16439730 SRX = SRX14894223 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2022-08-02T07:02:21 prefetch.2.10.7: 1) Downloading 'SRR18795798'... 2022-08-02T07:02:21 prefetch.2.10.7: Downloading via HTTPS... 2022-08-02T07:03:45 prefetch.2.10.7: HTTPS download succeed 2022-08-02T07:03:45 prefetch.2.10.7: 1) 'SRR18795798' was downloaded successfully 2022-08-02T07:03:45 prefetch.2.10.7: 'SRR18795798' has 0 unresolved dependencies Read 55739158 spots for SRR18795798/SRR18795798.sra Written 55739158 spots for SRR18795798/SRR18795798.sra fastq に変換しました。 bowtie でマッピング中... Your job 16439872 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:15:40 55739158 reads; of these: 55739158 (100.00%) were unpaired; of these: 4067946 (7.30%) aligned 0 times 37572712 (67.41%) aligned exactly 1 time 14098500 (25.29%) aligned >1 times 92.70% overall alignment rate Time searching: 00:15:40 Overall time: 00:15:40 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 24 files... [bam_rmdupse_core] 13022907 / 51671212 = 0.2520 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 16:33:04: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX14894223/SRX14894223.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX14894223/SRX14894223.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX14894223/SRX14894223.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX14894223/SRX14894223.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 16:33:04: #1 read tag files... INFO @ Tue, 02 Aug 2022 16:33:04: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 16:33:09: 1000000 INFO @ Tue, 02 Aug 2022 16:33:14: 2000000 INFO @ Tue, 02 Aug 2022 16:33:20: 3000000 INFO @ Tue, 02 Aug 2022 16:33:25: 4000000 INFO @ Tue, 02 Aug 2022 16:33:30: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 16:33:33: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX14894223/SRX14894223.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX14894223/SRX14894223.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX14894223/SRX14894223.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX14894223/SRX14894223.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 16:33:33: #1 read tag files... INFO @ Tue, 02 Aug 2022 16:33:33: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 16:33:36: 6000000 INFO @ Tue, 02 Aug 2022 16:33:38: 1000000 INFO @ Tue, 02 Aug 2022 16:33:41: 7000000 INFO @ Tue, 02 Aug 2022 16:33:43: 2000000 INFO @ Tue, 02 Aug 2022 16:33:46: 8000000 INFO @ Tue, 02 Aug 2022 16:33:49: 3000000 INFO @ Tue, 02 Aug 2022 16:33:52: 9000000 INFO @ Tue, 02 Aug 2022 16:33:54: 4000000 INFO @ Tue, 02 Aug 2022 16:33:57: 10000000 INFO @ Tue, 02 Aug 2022 16:33:59: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 16:34:02: 11000000 INFO @ Tue, 02 Aug 2022 16:34:03: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX14894223/SRX14894223.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX14894223/SRX14894223.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX14894223/SRX14894223.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX14894223/SRX14894223.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 16:34:03: #1 read tag files... INFO @ Tue, 02 Aug 2022 16:34:03: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 16:34:04: 6000000 INFO @ Tue, 02 Aug 2022 16:34:07: 12000000 INFO @ Tue, 02 Aug 2022 16:34:08: 1000000 INFO @ Tue, 02 Aug 2022 16:34:09: 7000000 INFO @ Tue, 02 Aug 2022 16:34:13: 13000000 INFO @ Tue, 02 Aug 2022 16:34:14: 2000000 INFO @ Tue, 02 Aug 2022 16:34:14: 8000000 INFO @ Tue, 02 Aug 2022 16:34:18: 14000000 INFO @ Tue, 02 Aug 2022 16:34:19: 3000000 INFO @ Tue, 02 Aug 2022 16:34:19: 9000000 INFO @ Tue, 02 Aug 2022 16:34:23: 15000000 INFO @ Tue, 02 Aug 2022 16:34:24: 4000000 INFO @ Tue, 02 Aug 2022 16:34:25: 10000000 INFO @ Tue, 02 Aug 2022 16:34:28: 16000000 INFO @ Tue, 02 Aug 2022 16:34:30: 11000000 INFO @ Tue, 02 Aug 2022 16:34:30: 5000000 INFO @ Tue, 02 Aug 2022 16:34:34: 17000000 INFO @ Tue, 02 Aug 2022 16:34:35: 12000000 INFO @ Tue, 02 Aug 2022 16:34:35: 6000000 INFO @ Tue, 02 Aug 2022 16:34:39: 18000000 INFO @ Tue, 02 Aug 2022 16:34:40: 13000000 INFO @ Tue, 02 Aug 2022 16:34:41: 7000000 INFO @ Tue, 02 Aug 2022 16:34:44: 19000000 INFO @ Tue, 02 Aug 2022 16:34:44: 14000000 INFO @ Tue, 02 Aug 2022 16:34:46: 8000000 INFO @ Tue, 02 Aug 2022 16:34:49: 15000000 INFO @ Tue, 02 Aug 2022 16:34:49: 20000000 INFO @ Tue, 02 Aug 2022 16:34:52: 9000000 INFO @ Tue, 02 Aug 2022 16:34:54: 16000000 INFO @ Tue, 02 Aug 2022 16:34:54: 21000000 INFO @ Tue, 02 Aug 2022 16:34:57: 10000000 INFO @ Tue, 02 Aug 2022 16:34:59: 17000000 INFO @ Tue, 02 Aug 2022 16:35:00: 22000000 INFO @ Tue, 02 Aug 2022 16:35:02: 11000000 INFO @ Tue, 02 Aug 2022 16:35:04: 18000000 INFO @ Tue, 02 Aug 2022 16:35:05: 23000000 INFO @ Tue, 02 Aug 2022 16:35:07: 12000000 INFO @ Tue, 02 Aug 2022 16:35:09: 19000000 INFO @ Tue, 02 Aug 2022 16:35:10: 24000000 INFO @ Tue, 02 Aug 2022 16:35:13: 13000000 INFO @ Tue, 02 Aug 2022 16:35:14: 20000000 INFO @ Tue, 02 Aug 2022 16:35:15: 25000000 INFO @ Tue, 02 Aug 2022 16:35:18: 14000000 INFO @ Tue, 02 Aug 2022 16:35:20: 21000000 INFO @ Tue, 02 Aug 2022 16:35:21: 26000000 INFO @ Tue, 02 Aug 2022 16:35:23: 15000000 INFO @ Tue, 02 Aug 2022 16:35:25: 22000000 INFO @ Tue, 02 Aug 2022 16:35:26: 27000000 INFO @ Tue, 02 Aug 2022 16:35:28: 16000000 INFO @ Tue, 02 Aug 2022 16:35:30: 23000000 INFO @ Tue, 02 Aug 2022 16:35:31: 28000000 INFO @ Tue, 02 Aug 2022 16:35:34: 17000000 INFO @ Tue, 02 Aug 2022 16:35:35: 24000000 INFO @ Tue, 02 Aug 2022 16:35:37: 29000000 INFO @ Tue, 02 Aug 2022 16:35:39: 18000000 INFO @ Tue, 02 Aug 2022 16:35:40: 25000000 INFO @ Tue, 02 Aug 2022 16:35:42: 30000000 INFO @ Tue, 02 Aug 2022 16:35:44: 19000000 INFO @ Tue, 02 Aug 2022 16:35:45: 26000000 INFO @ Tue, 02 Aug 2022 16:35:48: 31000000 INFO @ Tue, 02 Aug 2022 16:35:49: 20000000 INFO @ Tue, 02 Aug 2022 16:35:50: 27000000 INFO @ Tue, 02 Aug 2022 16:35:53: 32000000 INFO @ Tue, 02 Aug 2022 16:35:54: 21000000 INFO @ Tue, 02 Aug 2022 16:35:55: 28000000 INFO @ Tue, 02 Aug 2022 16:35:59: 33000000 INFO @ Tue, 02 Aug 2022 16:36:00: 22000000 INFO @ Tue, 02 Aug 2022 16:36:01: 29000000 INFO @ Tue, 02 Aug 2022 16:36:04: 34000000 INFO @ Tue, 02 Aug 2022 16:36:05: 23000000 INFO @ Tue, 02 Aug 2022 16:36:06: 30000000 INFO @ Tue, 02 Aug 2022 16:36:09: 35000000 INFO @ Tue, 02 Aug 2022 16:36:10: 24000000 INFO @ Tue, 02 Aug 2022 16:36:11: 31000000 INFO @ Tue, 02 Aug 2022 16:36:15: 36000000 INFO @ Tue, 02 Aug 2022 16:36:15: 25000000 INFO @ Tue, 02 Aug 2022 16:36:16: 32000000 INFO @ Tue, 02 Aug 2022 16:36:20: 37000000 INFO @ Tue, 02 Aug 2022 16:36:21: 26000000 INFO @ Tue, 02 Aug 2022 16:36:21: 33000000 INFO @ Tue, 02 Aug 2022 16:36:25: 38000000 INFO @ Tue, 02 Aug 2022 16:36:26: 27000000 INFO @ Tue, 02 Aug 2022 16:36:26: 34000000 INFO @ Tue, 02 Aug 2022 16:36:28: #1 tag size is determined as 51 bps INFO @ Tue, 02 Aug 2022 16:36:28: #1 tag size = 51 INFO @ Tue, 02 Aug 2022 16:36:28: #1 total tags in treatment: 38648305 INFO @ Tue, 02 Aug 2022 16:36:28: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 16:36:28: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 16:36:29: #1 tags after filtering in treatment: 38648305 INFO @ Tue, 02 Aug 2022 16:36:29: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 16:36:29: #1 finished! INFO @ Tue, 02 Aug 2022 16:36:29: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 16:36:29: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 16:36:31: 28000000 INFO @ Tue, 02 Aug 2022 16:36:31: 35000000 INFO @ Tue, 02 Aug 2022 16:36:31: #2 number of paired peaks: 36 WARNING @ Tue, 02 Aug 2022 16:36:31: Too few paired peaks (36) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 16:36:31: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX14894223/SRX14894223.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX14894223/SRX14894223.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX14894223/SRX14894223.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX14894223/SRX14894223.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 16:36:36: 36000000 INFO @ Tue, 02 Aug 2022 16:36:37: 29000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 02 Aug 2022 16:36:41: 37000000 INFO @ Tue, 02 Aug 2022 16:36:42: 30000000 INFO @ Tue, 02 Aug 2022 16:36:46: 38000000 INFO @ Tue, 02 Aug 2022 16:36:48: 31000000 INFO @ Tue, 02 Aug 2022 16:36:49: #1 tag size is determined as 51 bps INFO @ Tue, 02 Aug 2022 16:36:49: #1 tag size = 51 INFO @ Tue, 02 Aug 2022 16:36:49: #1 total tags in treatment: 38648305 INFO @ Tue, 02 Aug 2022 16:36:49: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 16:36:49: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 16:36:50: #1 tags after filtering in treatment: 38648305 INFO @ Tue, 02 Aug 2022 16:36:50: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 16:36:50: #1 finished! INFO @ Tue, 02 Aug 2022 16:36:50: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 16:36:50: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 16:36:52: #2 number of paired peaks: 36 WARNING @ Tue, 02 Aug 2022 16:36:52: Too few paired peaks (36) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 16:36:52: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX14894223/SRX14894223.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX14894223/SRX14894223.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX14894223/SRX14894223.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX14894223/SRX14894223.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 16:36:53: 32000000 INFO @ Tue, 02 Aug 2022 16:36:58: 33000000 INFO @ Tue, 02 Aug 2022 16:37:03: 34000000 INFO @ Tue, 02 Aug 2022 16:37:08: 35000000 INFO @ Tue, 02 Aug 2022 16:37:13: 36000000 INFO @ Tue, 02 Aug 2022 16:37:18: 37000000 INFO @ Tue, 02 Aug 2022 16:37:23: 38000000 INFO @ Tue, 02 Aug 2022 16:37:27: #1 tag size is determined as 51 bps INFO @ Tue, 02 Aug 2022 16:37:27: #1 tag size = 51 INFO @ Tue, 02 Aug 2022 16:37:27: #1 total tags in treatment: 38648305 INFO @ Tue, 02 Aug 2022 16:37:27: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 16:37:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 16:37:27: #1 tags after filtering in treatment: 38648305 INFO @ Tue, 02 Aug 2022 16:37:27: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 16:37:27: #1 finished! INFO @ Tue, 02 Aug 2022 16:37:27: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 16:37:27: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 16:37:30: #2 number of paired peaks: 36 WARNING @ Tue, 02 Aug 2022 16:37:30: Too few paired peaks (36) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 16:37:30: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX14894223/SRX14894223.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX14894223/SRX14894223.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX14894223/SRX14894223.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX14894223/SRX14894223.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。