Job ID = 16439729 SRX = SRX14894222 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2022-08-02T07:01:51 prefetch.2.10.7: 1) Downloading 'SRR18795799'... 2022-08-02T07:01:51 prefetch.2.10.7: Downloading via HTTPS... 2022-08-02T07:03:08 prefetch.2.10.7: HTTPS download succeed 2022-08-02T07:03:08 prefetch.2.10.7: 1) 'SRR18795799' was downloaded successfully 2022-08-02T07:03:08 prefetch.2.10.7: 'SRR18795799' has 0 unresolved dependencies Read 54351756 spots for SRR18795799/SRR18795799.sra Written 54351756 spots for SRR18795799/SRR18795799.sra fastq に変換しました。 bowtie でマッピング中... Your job 16439870 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:16:23 54351756 reads; of these: 54351756 (100.00%) were unpaired; of these: 3688698 (6.79%) aligned 0 times 37082197 (68.23%) aligned exactly 1 time 13580861 (24.99%) aligned >1 times 93.21% overall alignment rate Time searching: 00:16:23 Overall time: 00:16:23 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 24 files... [bam_rmdupse_core] 12974190 / 50663058 = 0.2561 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 16:32:51: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX14894222/SRX14894222.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX14894222/SRX14894222.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX14894222/SRX14894222.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX14894222/SRX14894222.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 16:32:51: #1 read tag files... INFO @ Tue, 02 Aug 2022 16:32:51: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 16:32:56: 1000000 INFO @ Tue, 02 Aug 2022 16:33:01: 2000000 INFO @ Tue, 02 Aug 2022 16:33:07: 3000000 INFO @ Tue, 02 Aug 2022 16:33:12: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 16:33:18: 5000000 INFO @ Tue, 02 Aug 2022 16:33:19: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX14894222/SRX14894222.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX14894222/SRX14894222.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX14894222/SRX14894222.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX14894222/SRX14894222.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 16:33:19: #1 read tag files... INFO @ Tue, 02 Aug 2022 16:33:19: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 16:33:23: 6000000 INFO @ Tue, 02 Aug 2022 16:33:25: 1000000 INFO @ Tue, 02 Aug 2022 16:33:28: 7000000 INFO @ Tue, 02 Aug 2022 16:33:31: 2000000 INFO @ Tue, 02 Aug 2022 16:33:34: 8000000 INFO @ Tue, 02 Aug 2022 16:33:36: 3000000 INFO @ Tue, 02 Aug 2022 16:33:39: 9000000 INFO @ Tue, 02 Aug 2022 16:33:41: 4000000 INFO @ Tue, 02 Aug 2022 16:33:44: 10000000 INFO @ Tue, 02 Aug 2022 16:33:47: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 16:33:49: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX14894222/SRX14894222.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX14894222/SRX14894222.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX14894222/SRX14894222.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX14894222/SRX14894222.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 16:33:49: #1 read tag files... INFO @ Tue, 02 Aug 2022 16:33:49: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 16:33:50: 11000000 INFO @ Tue, 02 Aug 2022 16:33:52: 6000000 INFO @ Tue, 02 Aug 2022 16:33:55: 1000000 INFO @ Tue, 02 Aug 2022 16:33:55: 12000000 INFO @ Tue, 02 Aug 2022 16:33:58: 7000000 INFO @ Tue, 02 Aug 2022 16:34:01: 13000000 INFO @ Tue, 02 Aug 2022 16:34:01: 2000000 INFO @ Tue, 02 Aug 2022 16:34:03: 8000000 INFO @ Tue, 02 Aug 2022 16:34:07: 14000000 INFO @ Tue, 02 Aug 2022 16:34:07: 3000000 INFO @ Tue, 02 Aug 2022 16:34:08: 9000000 INFO @ Tue, 02 Aug 2022 16:34:12: 15000000 INFO @ Tue, 02 Aug 2022 16:34:13: 4000000 INFO @ Tue, 02 Aug 2022 16:34:14: 10000000 INFO @ Tue, 02 Aug 2022 16:34:18: 16000000 INFO @ Tue, 02 Aug 2022 16:34:19: 5000000 INFO @ Tue, 02 Aug 2022 16:34:19: 11000000 INFO @ Tue, 02 Aug 2022 16:34:24: 17000000 INFO @ Tue, 02 Aug 2022 16:34:24: 12000000 INFO @ Tue, 02 Aug 2022 16:34:25: 6000000 INFO @ Tue, 02 Aug 2022 16:34:30: 18000000 INFO @ Tue, 02 Aug 2022 16:34:30: 13000000 INFO @ Tue, 02 Aug 2022 16:34:31: 7000000 INFO @ Tue, 02 Aug 2022 16:34:36: 19000000 INFO @ Tue, 02 Aug 2022 16:34:36: 14000000 INFO @ Tue, 02 Aug 2022 16:34:37: 8000000 INFO @ Tue, 02 Aug 2022 16:34:42: 15000000 INFO @ Tue, 02 Aug 2022 16:34:42: 20000000 INFO @ Tue, 02 Aug 2022 16:34:43: 9000000 INFO @ Tue, 02 Aug 2022 16:34:48: 21000000 INFO @ Tue, 02 Aug 2022 16:34:48: 16000000 INFO @ Tue, 02 Aug 2022 16:34:49: 10000000 INFO @ Tue, 02 Aug 2022 16:34:54: 17000000 INFO @ Tue, 02 Aug 2022 16:34:54: 22000000 INFO @ Tue, 02 Aug 2022 16:34:55: 11000000 INFO @ Tue, 02 Aug 2022 16:34:59: 23000000 INFO @ Tue, 02 Aug 2022 16:34:59: 18000000 INFO @ Tue, 02 Aug 2022 16:35:00: 12000000 INFO @ Tue, 02 Aug 2022 16:35:05: 24000000 INFO @ Tue, 02 Aug 2022 16:35:05: 19000000 INFO @ Tue, 02 Aug 2022 16:35:06: 13000000 INFO @ Tue, 02 Aug 2022 16:35:11: 25000000 INFO @ Tue, 02 Aug 2022 16:35:11: 20000000 INFO @ Tue, 02 Aug 2022 16:35:12: 14000000 INFO @ Tue, 02 Aug 2022 16:35:17: 26000000 INFO @ Tue, 02 Aug 2022 16:35:17: 21000000 INFO @ Tue, 02 Aug 2022 16:35:18: 15000000 INFO @ Tue, 02 Aug 2022 16:35:23: 27000000 INFO @ Tue, 02 Aug 2022 16:35:23: 22000000 INFO @ Tue, 02 Aug 2022 16:35:24: 16000000 INFO @ Tue, 02 Aug 2022 16:35:29: 23000000 INFO @ Tue, 02 Aug 2022 16:35:29: 28000000 INFO @ Tue, 02 Aug 2022 16:35:30: 17000000 INFO @ Tue, 02 Aug 2022 16:35:34: 24000000 INFO @ Tue, 02 Aug 2022 16:35:34: 29000000 INFO @ Tue, 02 Aug 2022 16:35:36: 18000000 INFO @ Tue, 02 Aug 2022 16:35:40: 25000000 INFO @ Tue, 02 Aug 2022 16:35:40: 30000000 INFO @ Tue, 02 Aug 2022 16:35:42: 19000000 INFO @ Tue, 02 Aug 2022 16:35:46: 26000000 INFO @ Tue, 02 Aug 2022 16:35:46: 31000000 INFO @ Tue, 02 Aug 2022 16:35:48: 20000000 INFO @ Tue, 02 Aug 2022 16:35:52: 27000000 INFO @ Tue, 02 Aug 2022 16:35:52: 32000000 INFO @ Tue, 02 Aug 2022 16:35:54: 21000000 INFO @ Tue, 02 Aug 2022 16:35:58: 28000000 INFO @ Tue, 02 Aug 2022 16:35:58: 33000000 INFO @ Tue, 02 Aug 2022 16:36:00: 22000000 INFO @ Tue, 02 Aug 2022 16:36:03: 29000000 INFO @ Tue, 02 Aug 2022 16:36:03: 34000000 INFO @ Tue, 02 Aug 2022 16:36:05: 23000000 INFO @ Tue, 02 Aug 2022 16:36:09: 30000000 INFO @ Tue, 02 Aug 2022 16:36:09: 35000000 INFO @ Tue, 02 Aug 2022 16:36:11: 24000000 INFO @ Tue, 02 Aug 2022 16:36:15: 31000000 INFO @ Tue, 02 Aug 2022 16:36:15: 36000000 INFO @ Tue, 02 Aug 2022 16:36:17: 25000000 INFO @ Tue, 02 Aug 2022 16:36:21: 37000000 INFO @ Tue, 02 Aug 2022 16:36:21: 32000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 02 Aug 2022 16:36:23: 26000000 INFO @ Tue, 02 Aug 2022 16:36:25: #1 tag size is determined as 51 bps INFO @ Tue, 02 Aug 2022 16:36:25: #1 tag size = 51 INFO @ Tue, 02 Aug 2022 16:36:25: #1 total tags in treatment: 37688868 INFO @ Tue, 02 Aug 2022 16:36:25: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 16:36:25: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 16:36:26: #1 tags after filtering in treatment: 37688868 INFO @ Tue, 02 Aug 2022 16:36:26: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 16:36:26: #1 finished! INFO @ Tue, 02 Aug 2022 16:36:26: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 16:36:26: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 16:36:27: 33000000 INFO @ Tue, 02 Aug 2022 16:36:28: #2 number of paired peaks: 29 WARNING @ Tue, 02 Aug 2022 16:36:28: Too few paired peaks (29) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 16:36:28: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX14894222/SRX14894222.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX14894222/SRX14894222.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX14894222/SRX14894222.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX14894222/SRX14894222.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 16:36:29: 27000000 INFO @ Tue, 02 Aug 2022 16:36:33: 34000000 INFO @ Tue, 02 Aug 2022 16:36:35: 28000000 INFO @ Tue, 02 Aug 2022 16:36:38: 35000000 INFO @ Tue, 02 Aug 2022 16:36:41: 29000000 INFO @ Tue, 02 Aug 2022 16:36:44: 36000000 INFO @ Tue, 02 Aug 2022 16:36:46: 30000000 INFO @ Tue, 02 Aug 2022 16:36:50: 37000000 INFO @ Tue, 02 Aug 2022 16:36:52: 31000000 INFO @ Tue, 02 Aug 2022 16:36:54: #1 tag size is determined as 51 bps INFO @ Tue, 02 Aug 2022 16:36:54: #1 tag size = 51 INFO @ Tue, 02 Aug 2022 16:36:54: #1 total tags in treatment: 37688868 INFO @ Tue, 02 Aug 2022 16:36:54: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 16:36:54: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 16:36:55: #1 tags after filtering in treatment: 37688868 INFO @ Tue, 02 Aug 2022 16:36:55: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 16:36:55: #1 finished! INFO @ Tue, 02 Aug 2022 16:36:55: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 16:36:55: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 16:36:57: #2 number of paired peaks: 29 WARNING @ Tue, 02 Aug 2022 16:36:57: Too few paired peaks (29) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 16:36:57: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX14894222/SRX14894222.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX14894222/SRX14894222.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX14894222/SRX14894222.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX14894222/SRX14894222.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 16:36:58: 32000000 INFO @ Tue, 02 Aug 2022 16:37:03: 33000000 INFO @ Tue, 02 Aug 2022 16:37:09: 34000000 INFO @ Tue, 02 Aug 2022 16:37:15: 35000000 INFO @ Tue, 02 Aug 2022 16:37:20: 36000000 INFO @ Tue, 02 Aug 2022 16:37:26: 37000000 INFO @ Tue, 02 Aug 2022 16:37:30: #1 tag size is determined as 51 bps INFO @ Tue, 02 Aug 2022 16:37:30: #1 tag size = 51 INFO @ Tue, 02 Aug 2022 16:37:30: #1 total tags in treatment: 37688868 INFO @ Tue, 02 Aug 2022 16:37:30: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 16:37:30: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 16:37:30: #1 tags after filtering in treatment: 37688868 INFO @ Tue, 02 Aug 2022 16:37:30: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 16:37:30: #1 finished! INFO @ Tue, 02 Aug 2022 16:37:30: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 16:37:30: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 16:37:33: #2 number of paired peaks: 29 WARNING @ Tue, 02 Aug 2022 16:37:33: Too few paired peaks (29) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 16:37:33: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX14894222/SRX14894222.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX14894222/SRX14894222.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX14894222/SRX14894222.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX14894222/SRX14894222.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。