Job ID = 1293901 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2019-06-02T18:11:20 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-06-02T18:11:20 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-06-02T18:11:20 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) spots read : 64,681,536 reads read : 129,363,072 reads written : 129,363,072 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 06:48:52 64681536 reads; of these: 64681536 (100.00%) were paired; of these: 4721601 (7.30%) aligned concordantly 0 times 31903212 (49.32%) aligned concordantly exactly 1 time 28056723 (43.38%) aligned concordantly >1 times ---- 4721601 pairs aligned concordantly 0 times; of these: 1040043 (22.03%) aligned discordantly 1 time ---- 3681558 pairs aligned 0 times concordantly or discordantly; of these: 7363116 mates make up the pairs; of these: 4891008 (66.43%) aligned 0 times 881043 (11.97%) aligned exactly 1 time 1591065 (21.61%) aligned >1 times 96.22% overall alignment rate Time searching: 06:48:52 Overall time: 06:48:52 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 56 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 27751741 / 60476770 = 0.4589 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 03 Jun 2019 10:59:00: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX146997/SRX146997.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX146997/SRX146997.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX146997/SRX146997.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX146997/SRX146997.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 10:59:00: #1 read tag files... INFO @ Mon, 03 Jun 2019 10:59:00: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 10:59:00: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX146997/SRX146997.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX146997/SRX146997.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX146997/SRX146997.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX146997/SRX146997.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 10:59:00: #1 read tag files... INFO @ Mon, 03 Jun 2019 10:59:00: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 10:59:00: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX146997/SRX146997.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX146997/SRX146997.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX146997/SRX146997.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX146997/SRX146997.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 10:59:00: #1 read tag files... INFO @ Mon, 03 Jun 2019 10:59:00: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 10:59:09: 1000000 INFO @ Mon, 03 Jun 2019 10:59:10: 1000000 INFO @ Mon, 03 Jun 2019 10:59:11: 1000000 INFO @ Mon, 03 Jun 2019 10:59:17: 2000000 INFO @ Mon, 03 Jun 2019 10:59:19: 2000000 INFO @ Mon, 03 Jun 2019 10:59:21: 2000000 INFO @ Mon, 03 Jun 2019 10:59:25: 3000000 INFO @ Mon, 03 Jun 2019 10:59:28: 3000000 INFO @ Mon, 03 Jun 2019 10:59:32: 3000000 INFO @ Mon, 03 Jun 2019 10:59:33: 4000000 INFO @ Mon, 03 Jun 2019 10:59:37: 4000000 INFO @ Mon, 03 Jun 2019 10:59:41: 5000000 INFO @ Mon, 03 Jun 2019 10:59:43: 4000000 INFO @ Mon, 03 Jun 2019 10:59:45: 5000000 INFO @ Mon, 03 Jun 2019 10:59:49: 6000000 INFO @ Mon, 03 Jun 2019 10:59:53: 5000000 INFO @ Mon, 03 Jun 2019 10:59:55: 6000000 INFO @ Mon, 03 Jun 2019 10:59:57: 7000000 INFO @ Mon, 03 Jun 2019 11:00:03: 7000000 INFO @ Mon, 03 Jun 2019 11:00:04: 6000000 INFO @ Mon, 03 Jun 2019 11:00:05: 8000000 INFO @ Mon, 03 Jun 2019 11:00:12: 8000000 INFO @ Mon, 03 Jun 2019 11:00:13: 7000000 INFO @ Mon, 03 Jun 2019 11:00:13: 9000000 INFO @ Mon, 03 Jun 2019 11:00:20: 9000000 INFO @ Mon, 03 Jun 2019 11:00:22: 10000000 INFO @ Mon, 03 Jun 2019 11:00:23: 8000000 INFO @ Mon, 03 Jun 2019 11:00:29: 10000000 INFO @ Mon, 03 Jun 2019 11:00:31: 11000000 INFO @ Mon, 03 Jun 2019 11:00:33: 9000000 INFO @ Mon, 03 Jun 2019 11:00:38: 11000000 INFO @ Mon, 03 Jun 2019 11:00:40: 12000000 INFO @ Mon, 03 Jun 2019 11:00:42: 10000000 INFO @ Mon, 03 Jun 2019 11:00:47: 12000000 INFO @ Mon, 03 Jun 2019 11:00:48: 13000000 INFO @ Mon, 03 Jun 2019 11:00:53: 11000000 INFO @ Mon, 03 Jun 2019 11:00:56: 13000000 INFO @ Mon, 03 Jun 2019 11:00:57: 14000000 INFO @ Mon, 03 Jun 2019 11:01:04: 12000000 INFO @ Mon, 03 Jun 2019 11:01:04: 14000000 INFO @ Mon, 03 Jun 2019 11:01:06: 15000000 INFO @ Mon, 03 Jun 2019 11:01:13: 15000000 INFO @ Mon, 03 Jun 2019 11:01:13: 16000000 INFO @ Mon, 03 Jun 2019 11:01:13: 13000000 INFO @ Mon, 03 Jun 2019 11:01:21: 17000000 INFO @ Mon, 03 Jun 2019 11:01:21: 16000000 INFO @ Mon, 03 Jun 2019 11:01:23: 14000000 INFO @ Mon, 03 Jun 2019 11:01:29: 18000000 INFO @ Mon, 03 Jun 2019 11:01:30: 17000000 INFO @ Mon, 03 Jun 2019 11:01:33: 15000000 INFO @ Mon, 03 Jun 2019 11:01:37: 19000000 INFO @ Mon, 03 Jun 2019 11:01:38: 18000000 INFO @ Mon, 03 Jun 2019 11:01:43: 16000000 INFO @ Mon, 03 Jun 2019 11:01:44: 20000000 INFO @ Mon, 03 Jun 2019 11:01:46: 19000000 INFO @ Mon, 03 Jun 2019 11:01:52: 21000000 INFO @ Mon, 03 Jun 2019 11:01:53: 17000000 INFO @ Mon, 03 Jun 2019 11:01:55: 20000000 INFO @ Mon, 03 Jun 2019 11:02:00: 22000000 INFO @ Mon, 03 Jun 2019 11:02:03: 18000000 INFO @ Mon, 03 Jun 2019 11:02:03: 21000000 INFO @ Mon, 03 Jun 2019 11:02:07: 23000000 INFO @ Mon, 03 Jun 2019 11:02:12: 22000000 INFO @ Mon, 03 Jun 2019 11:02:12: 19000000 INFO @ Mon, 03 Jun 2019 11:02:15: 24000000 INFO @ Mon, 03 Jun 2019 11:02:20: 23000000 INFO @ Mon, 03 Jun 2019 11:02:22: 20000000 INFO @ Mon, 03 Jun 2019 11:02:22: 25000000 INFO @ Mon, 03 Jun 2019 11:02:28: 24000000 INFO @ Mon, 03 Jun 2019 11:02:30: 26000000 INFO @ Mon, 03 Jun 2019 11:02:31: 21000000 INFO @ Mon, 03 Jun 2019 11:02:36: 25000000 INFO @ Mon, 03 Jun 2019 11:02:37: 27000000 INFO @ Mon, 03 Jun 2019 11:02:41: 22000000 INFO @ Mon, 03 Jun 2019 11:02:44: 26000000 INFO @ Mon, 03 Jun 2019 11:02:45: 28000000 INFO @ Mon, 03 Jun 2019 11:02:51: 23000000 INFO @ Mon, 03 Jun 2019 11:02:53: 29000000 INFO @ Mon, 03 Jun 2019 11:02:53: 27000000 INFO @ Mon, 03 Jun 2019 11:03:00: 30000000 INFO @ Mon, 03 Jun 2019 11:03:00: 24000000 INFO @ Mon, 03 Jun 2019 11:03:01: 28000000 INFO @ Mon, 03 Jun 2019 11:03:08: 31000000 INFO @ Mon, 03 Jun 2019 11:03:10: 25000000 INFO @ Mon, 03 Jun 2019 11:03:10: 29000000 INFO @ Mon, 03 Jun 2019 11:03:16: 32000000 INFO @ Mon, 03 Jun 2019 11:03:18: 30000000 INFO @ Mon, 03 Jun 2019 11:03:20: 26000000 INFO @ Mon, 03 Jun 2019 11:03:23: 33000000 INFO @ Mon, 03 Jun 2019 11:03:27: 31000000 INFO @ Mon, 03 Jun 2019 11:03:29: 27000000 INFO @ Mon, 03 Jun 2019 11:03:31: 34000000 INFO @ Mon, 03 Jun 2019 11:03:35: 32000000 INFO @ Mon, 03 Jun 2019 11:03:39: 28000000 INFO @ Mon, 03 Jun 2019 11:03:39: 35000000 INFO @ Mon, 03 Jun 2019 11:03:43: 33000000 INFO @ Mon, 03 Jun 2019 11:03:46: 36000000 INFO @ Mon, 03 Jun 2019 11:03:48: 29000000 INFO @ Mon, 03 Jun 2019 11:03:51: 34000000 INFO @ Mon, 03 Jun 2019 11:03:54: 37000000 INFO @ Mon, 03 Jun 2019 11:03:58: 30000000 INFO @ Mon, 03 Jun 2019 11:04:00: 35000000 INFO @ Mon, 03 Jun 2019 11:04:02: 38000000 INFO @ Mon, 03 Jun 2019 11:04:07: 31000000 INFO @ Mon, 03 Jun 2019 11:04:08: 36000000 INFO @ Mon, 03 Jun 2019 11:04:10: 39000000 INFO @ Mon, 03 Jun 2019 11:04:16: 37000000 INFO @ Mon, 03 Jun 2019 11:04:17: 32000000 INFO @ Mon, 03 Jun 2019 11:04:18: 40000000 INFO @ Mon, 03 Jun 2019 11:04:24: 38000000 INFO @ Mon, 03 Jun 2019 11:04:26: 41000000 INFO @ Mon, 03 Jun 2019 11:04:27: 33000000 INFO @ Mon, 03 Jun 2019 11:04:33: 39000000 INFO @ Mon, 03 Jun 2019 11:04:34: 42000000 INFO @ Mon, 03 Jun 2019 11:04:37: 34000000 INFO @ Mon, 03 Jun 2019 11:04:41: 40000000 INFO @ Mon, 03 Jun 2019 11:04:41: 43000000 INFO @ Mon, 03 Jun 2019 11:04:46: 35000000 INFO @ Mon, 03 Jun 2019 11:04:49: 44000000 INFO @ Mon, 03 Jun 2019 11:04:50: 41000000 INFO @ Mon, 03 Jun 2019 11:04:56: 36000000 INFO @ Mon, 03 Jun 2019 11:04:57: 45000000 INFO @ Mon, 03 Jun 2019 11:04:59: 42000000 INFO @ Mon, 03 Jun 2019 11:05:05: 46000000 INFO @ Mon, 03 Jun 2019 11:05:06: 37000000 INFO @ Mon, 03 Jun 2019 11:05:08: 43000000 INFO @ Mon, 03 Jun 2019 11:05:13: 47000000 INFO @ Mon, 03 Jun 2019 11:05:15: 38000000 INFO @ Mon, 03 Jun 2019 11:05:18: 44000000 INFO @ Mon, 03 Jun 2019 11:05:21: 48000000 INFO @ Mon, 03 Jun 2019 11:05:26: 39000000 INFO @ Mon, 03 Jun 2019 11:05:27: 45000000 INFO @ Mon, 03 Jun 2019 11:05:30: 49000000 INFO @ Mon, 03 Jun 2019 11:05:36: 46000000 INFO @ Mon, 03 Jun 2019 11:05:36: 40000000 INFO @ Mon, 03 Jun 2019 11:05:38: 50000000 INFO @ Mon, 03 Jun 2019 11:05:45: 47000000 INFO @ Mon, 03 Jun 2019 11:05:46: 51000000 INFO @ Mon, 03 Jun 2019 11:05:47: 41000000 INFO @ Mon, 03 Jun 2019 11:05:54: 48000000 INFO @ Mon, 03 Jun 2019 11:05:54: 52000000 INFO @ Mon, 03 Jun 2019 11:05:57: 42000000 INFO @ Mon, 03 Jun 2019 11:06:03: 53000000 INFO @ Mon, 03 Jun 2019 11:06:04: 49000000 INFO @ Mon, 03 Jun 2019 11:06:08: 43000000 INFO @ Mon, 03 Jun 2019 11:06:11: 54000000 INFO @ Mon, 03 Jun 2019 11:06:14: 50000000 INFO @ Mon, 03 Jun 2019 11:06:18: 44000000 INFO @ Mon, 03 Jun 2019 11:06:19: 55000000 INFO @ Mon, 03 Jun 2019 11:06:23: 51000000 INFO @ Mon, 03 Jun 2019 11:06:28: 56000000 INFO @ Mon, 03 Jun 2019 11:06:28: 45000000 INFO @ Mon, 03 Jun 2019 11:06:33: 52000000 INFO @ Mon, 03 Jun 2019 11:06:36: 57000000 INFO @ Mon, 03 Jun 2019 11:06:38: 46000000 INFO @ Mon, 03 Jun 2019 11:06:43: 53000000 INFO @ Mon, 03 Jun 2019 11:06:45: 58000000 INFO @ Mon, 03 Jun 2019 11:06:48: 47000000 INFO @ Mon, 03 Jun 2019 11:06:52: 54000000 INFO @ Mon, 03 Jun 2019 11:06:53: 59000000 INFO @ Mon, 03 Jun 2019 11:06:59: 48000000 INFO @ Mon, 03 Jun 2019 11:07:01: 55000000 INFO @ Mon, 03 Jun 2019 11:07:02: 60000000 INFO @ Mon, 03 Jun 2019 11:07:10: 56000000 INFO @ Mon, 03 Jun 2019 11:07:10: 61000000 INFO @ Mon, 03 Jun 2019 11:07:10: 49000000 INFO @ Mon, 03 Jun 2019 11:07:18: 62000000 INFO @ Mon, 03 Jun 2019 11:07:19: 57000000 INFO @ Mon, 03 Jun 2019 11:07:22: 50000000 INFO @ Mon, 03 Jun 2019 11:07:27: 63000000 INFO @ Mon, 03 Jun 2019 11:07:27: 58000000 INFO @ Mon, 03 Jun 2019 11:07:33: 51000000 INFO @ Mon, 03 Jun 2019 11:07:35: 64000000 INFO @ Mon, 03 Jun 2019 11:07:36: 59000000 INFO @ Mon, 03 Jun 2019 11:07:43: 65000000 INFO @ Mon, 03 Jun 2019 11:07:44: 52000000 INFO @ Mon, 03 Jun 2019 11:07:45: 60000000 INFO @ Mon, 03 Jun 2019 11:07:51: 66000000 INFO @ Mon, 03 Jun 2019 11:07:53: 61000000 INFO @ Mon, 03 Jun 2019 11:07:54: 53000000 INFO @ Mon, 03 Jun 2019 11:07:59: 67000000 INFO @ Mon, 03 Jun 2019 11:08:02: 62000000 INFO @ Mon, 03 Jun 2019 11:08:05: 54000000 INFO @ Mon, 03 Jun 2019 11:08:07: 68000000 INFO @ Mon, 03 Jun 2019 11:08:11: 63000000 INFO @ Mon, 03 Jun 2019 11:08:15: #1 tag size is determined as 100 bps INFO @ Mon, 03 Jun 2019 11:08:15: #1 tag size = 100 INFO @ Mon, 03 Jun 2019 11:08:15: #1 total tags in treatment: 32654933 INFO @ Mon, 03 Jun 2019 11:08:15: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 11:08:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 11:08:15: 55000000 INFO @ Mon, 03 Jun 2019 11:08:16: #1 tags after filtering in treatment: 24988992 INFO @ Mon, 03 Jun 2019 11:08:16: #1 Redundant rate of treatment: 0.23 INFO @ Mon, 03 Jun 2019 11:08:16: #1 finished! INFO @ Mon, 03 Jun 2019 11:08:16: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 11:08:16: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 11:08:18: #2 number of paired peaks: 1543 INFO @ Mon, 03 Jun 2019 11:08:18: start model_add_line... INFO @ Mon, 03 Jun 2019 11:08:18: start X-correlation... INFO @ Mon, 03 Jun 2019 11:08:18: end of X-cor INFO @ Mon, 03 Jun 2019 11:08:18: #2 finished! INFO @ Mon, 03 Jun 2019 11:08:18: #2 predicted fragment length is 130 bps INFO @ Mon, 03 Jun 2019 11:08:18: #2 alternative fragment length(s) may be 4,130 bps INFO @ Mon, 03 Jun 2019 11:08:18: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX146997/SRX146997.05_model.r WARNING @ Mon, 03 Jun 2019 11:08:18: #2 Since the d (130) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Mon, 03 Jun 2019 11:08:18: #2 You may need to consider one of the other alternative d(s): 4,130 WARNING @ Mon, 03 Jun 2019 11:08:18: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Mon, 03 Jun 2019 11:08:18: #3 Call peaks... INFO @ Mon, 03 Jun 2019 11:08:18: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 03 Jun 2019 11:08:20: 64000000 INFO @ Mon, 03 Jun 2019 11:08:26: 56000000 INFO @ Mon, 03 Jun 2019 11:08:29: 65000000 INFO @ Mon, 03 Jun 2019 11:08:36: 57000000 INFO @ Mon, 03 Jun 2019 11:08:37: 66000000 INFO @ Mon, 03 Jun 2019 11:08:45: 67000000 INFO @ Mon, 03 Jun 2019 11:08:46: 58000000 INFO @ Mon, 03 Jun 2019 11:08:54: 68000000 INFO @ Mon, 03 Jun 2019 11:08:57: 59000000 INFO @ Mon, 03 Jun 2019 11:09:02: #1 tag size is determined as 100 bps INFO @ Mon, 03 Jun 2019 11:09:02: #1 tag size = 100 INFO @ Mon, 03 Jun 2019 11:09:02: #1 total tags in treatment: 32654933 INFO @ Mon, 03 Jun 2019 11:09:02: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 11:09:02: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 11:09:03: #1 tags after filtering in treatment: 24988992 INFO @ Mon, 03 Jun 2019 11:09:03: #1 Redundant rate of treatment: 0.23 INFO @ Mon, 03 Jun 2019 11:09:03: #1 finished! INFO @ Mon, 03 Jun 2019 11:09:03: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 11:09:03: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 11:09:05: #2 number of paired peaks: 1543 INFO @ Mon, 03 Jun 2019 11:09:05: start model_add_line... INFO @ Mon, 03 Jun 2019 11:09:05: start X-correlation... INFO @ Mon, 03 Jun 2019 11:09:05: end of X-cor INFO @ Mon, 03 Jun 2019 11:09:05: #2 finished! INFO @ Mon, 03 Jun 2019 11:09:05: #2 predicted fragment length is 130 bps INFO @ Mon, 03 Jun 2019 11:09:05: #2 alternative fragment length(s) may be 4,130 bps INFO @ Mon, 03 Jun 2019 11:09:05: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX146997/SRX146997.20_model.r WARNING @ Mon, 03 Jun 2019 11:09:05: #2 Since the d (130) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Mon, 03 Jun 2019 11:09:05: #2 You may need to consider one of the other alternative d(s): 4,130 WARNING @ Mon, 03 Jun 2019 11:09:05: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Mon, 03 Jun 2019 11:09:05: #3 Call peaks... INFO @ Mon, 03 Jun 2019 11:09:05: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 03 Jun 2019 11:09:07: 60000000 INFO @ Mon, 03 Jun 2019 11:09:17: 61000000 INFO @ Mon, 03 Jun 2019 11:09:27: #3 Call peaks for each chromosome... INFO @ Mon, 03 Jun 2019 11:09:27: 62000000 INFO @ Mon, 03 Jun 2019 11:09:38: 63000000 INFO @ Mon, 03 Jun 2019 11:09:48: 64000000 INFO @ Mon, 03 Jun 2019 11:09:56: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX146997/SRX146997.05_peaks.xls INFO @ Mon, 03 Jun 2019 11:09:57: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX146997/SRX146997.05_peaks.narrowPeak INFO @ Mon, 03 Jun 2019 11:09:57: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX146997/SRX146997.05_summits.bed INFO @ Mon, 03 Jun 2019 11:09:57: Done! pass1 - making usageList (15 chroms): 3 millis pass2 - checking and writing primary data (13188 records, 4 fields): 17 millis CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 11:09:57: 65000000 INFO @ Mon, 03 Jun 2019 11:10:07: 66000000 INFO @ Mon, 03 Jun 2019 11:10:14: #3 Call peaks for each chromosome... INFO @ Mon, 03 Jun 2019 11:10:18: 67000000 INFO @ Mon, 03 Jun 2019 11:10:27: 68000000 INFO @ Mon, 03 Jun 2019 11:10:37: #1 tag size is determined as 100 bps INFO @ Mon, 03 Jun 2019 11:10:37: #1 tag size = 100 INFO @ Mon, 03 Jun 2019 11:10:37: #1 total tags in treatment: 32654933 INFO @ Mon, 03 Jun 2019 11:10:37: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 11:10:37: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 11:10:38: #1 tags after filtering in treatment: 24988992 INFO @ Mon, 03 Jun 2019 11:10:38: #1 Redundant rate of treatment: 0.23 INFO @ Mon, 03 Jun 2019 11:10:38: #1 finished! INFO @ Mon, 03 Jun 2019 11:10:38: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 11:10:38: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 11:10:40: #2 number of paired peaks: 1543 INFO @ Mon, 03 Jun 2019 11:10:40: start model_add_line... INFO @ Mon, 03 Jun 2019 11:10:41: start X-correlation... INFO @ Mon, 03 Jun 2019 11:10:41: end of X-cor INFO @ Mon, 03 Jun 2019 11:10:41: #2 finished! INFO @ Mon, 03 Jun 2019 11:10:41: #2 predicted fragment length is 130 bps INFO @ Mon, 03 Jun 2019 11:10:41: #2 alternative fragment length(s) may be 4,130 bps INFO @ Mon, 03 Jun 2019 11:10:41: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX146997/SRX146997.10_model.r WARNING @ Mon, 03 Jun 2019 11:10:41: #2 Since the d (130) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Mon, 03 Jun 2019 11:10:41: #2 You may need to consider one of the other alternative d(s): 4,130 WARNING @ Mon, 03 Jun 2019 11:10:41: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Mon, 03 Jun 2019 11:10:41: #3 Call peaks... INFO @ Mon, 03 Jun 2019 11:10:41: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 03 Jun 2019 11:10:44: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX146997/SRX146997.20_peaks.xls INFO @ Mon, 03 Jun 2019 11:10:44: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX146997/SRX146997.20_peaks.narrowPeak INFO @ Mon, 03 Jun 2019 11:10:44: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX146997/SRX146997.20_summits.bed INFO @ Mon, 03 Jun 2019 11:10:44: Done! pass1 - making usageList (14 chroms): 2 millis pass2 - checking and writing primary data (4410 records, 4 fields): 14 millis CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 11:11:49: #3 Call peaks for each chromosome... INFO @ Mon, 03 Jun 2019 11:12:18: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX146997/SRX146997.10_peaks.xls INFO @ Mon, 03 Jun 2019 11:12:19: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX146997/SRX146997.10_peaks.narrowPeak INFO @ Mon, 03 Jun 2019 11:12:19: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX146997/SRX146997.10_summits.bed INFO @ Mon, 03 Jun 2019 11:12:19: Done! pass1 - making usageList (15 chroms): 5 millis pass2 - checking and writing primary data (8968 records, 4 fields): 14 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。