Job ID = 6527611 SRX = SRX1433359 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T13:05:55 prefetch.2.10.7: 1) Downloading 'SRR2919824'... 2020-06-29T13:05:55 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T13:11:11 prefetch.2.10.7: HTTPS download succeed 2020-06-29T13:11:11 prefetch.2.10.7: 1) 'SRR2919824' was downloaded successfully Read 44281887 spots for SRR2919824/SRR2919824.sra Written 44281887 spots for SRR2919824/SRR2919824.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:13:24 44281887 reads; of these: 44281887 (100.00%) were unpaired; of these: 1200492 (2.71%) aligned 0 times 32086590 (72.46%) aligned exactly 1 time 10994805 (24.83%) aligned >1 times 97.29% overall alignment rate Time searching: 00:13:24 Overall time: 00:13:24 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 13190323 / 43081395 = 0.3062 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 22:50:05: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX1433359/SRX1433359.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX1433359/SRX1433359.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX1433359/SRX1433359.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX1433359/SRX1433359.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 22:50:05: #1 read tag files... INFO @ Mon, 29 Jun 2020 22:50:05: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 22:50:12: 1000000 INFO @ Mon, 29 Jun 2020 22:50:18: 2000000 INFO @ Mon, 29 Jun 2020 22:50:25: 3000000 INFO @ Mon, 29 Jun 2020 22:50:31: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 22:50:35: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX1433359/SRX1433359.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX1433359/SRX1433359.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX1433359/SRX1433359.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX1433359/SRX1433359.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 22:50:35: #1 read tag files... INFO @ Mon, 29 Jun 2020 22:50:35: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 22:50:38: 5000000 INFO @ Mon, 29 Jun 2020 22:50:43: 1000000 INFO @ Mon, 29 Jun 2020 22:50:45: 6000000 INFO @ Mon, 29 Jun 2020 22:50:50: 2000000 INFO @ Mon, 29 Jun 2020 22:50:52: 7000000 INFO @ Mon, 29 Jun 2020 22:50:57: 3000000 INFO @ Mon, 29 Jun 2020 22:50:59: 8000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 22:51:04: 4000000 INFO @ Mon, 29 Jun 2020 22:51:05: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX1433359/SRX1433359.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX1433359/SRX1433359.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX1433359/SRX1433359.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX1433359/SRX1433359.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 22:51:05: #1 read tag files... INFO @ Mon, 29 Jun 2020 22:51:05: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 22:51:06: 9000000 INFO @ Mon, 29 Jun 2020 22:51:11: 5000000 INFO @ Mon, 29 Jun 2020 22:51:13: 1000000 INFO @ Mon, 29 Jun 2020 22:51:13: 10000000 INFO @ Mon, 29 Jun 2020 22:51:18: 6000000 INFO @ Mon, 29 Jun 2020 22:51:20: 2000000 INFO @ Mon, 29 Jun 2020 22:51:20: 11000000 INFO @ Mon, 29 Jun 2020 22:51:25: 7000000 INFO @ Mon, 29 Jun 2020 22:51:27: 3000000 INFO @ Mon, 29 Jun 2020 22:51:28: 12000000 INFO @ Mon, 29 Jun 2020 22:51:32: 8000000 INFO @ Mon, 29 Jun 2020 22:51:34: 4000000 INFO @ Mon, 29 Jun 2020 22:51:35: 13000000 INFO @ Mon, 29 Jun 2020 22:51:39: 9000000 INFO @ Mon, 29 Jun 2020 22:51:41: 5000000 INFO @ Mon, 29 Jun 2020 22:51:42: 14000000 INFO @ Mon, 29 Jun 2020 22:51:46: 10000000 INFO @ Mon, 29 Jun 2020 22:51:49: 6000000 INFO @ Mon, 29 Jun 2020 22:51:49: 15000000 INFO @ Mon, 29 Jun 2020 22:51:53: 11000000 INFO @ Mon, 29 Jun 2020 22:51:56: 16000000 INFO @ Mon, 29 Jun 2020 22:51:57: 7000000 INFO @ Mon, 29 Jun 2020 22:52:01: 12000000 INFO @ Mon, 29 Jun 2020 22:52:03: 17000000 INFO @ Mon, 29 Jun 2020 22:52:04: 8000000 INFO @ Mon, 29 Jun 2020 22:52:08: 13000000 INFO @ Mon, 29 Jun 2020 22:52:10: 18000000 INFO @ Mon, 29 Jun 2020 22:52:11: 9000000 INFO @ Mon, 29 Jun 2020 22:52:15: 14000000 INFO @ Mon, 29 Jun 2020 22:52:17: 19000000 INFO @ Mon, 29 Jun 2020 22:52:18: 10000000 INFO @ Mon, 29 Jun 2020 22:52:22: 15000000 INFO @ Mon, 29 Jun 2020 22:52:24: 20000000 INFO @ Mon, 29 Jun 2020 22:52:25: 11000000 INFO @ Mon, 29 Jun 2020 22:52:29: 16000000 INFO @ Mon, 29 Jun 2020 22:52:31: 21000000 INFO @ Mon, 29 Jun 2020 22:52:32: 12000000 INFO @ Mon, 29 Jun 2020 22:52:36: 17000000 INFO @ Mon, 29 Jun 2020 22:52:38: 22000000 INFO @ Mon, 29 Jun 2020 22:52:39: 13000000 INFO @ Mon, 29 Jun 2020 22:52:43: 18000000 INFO @ Mon, 29 Jun 2020 22:52:46: 23000000 INFO @ Mon, 29 Jun 2020 22:52:46: 14000000 INFO @ Mon, 29 Jun 2020 22:52:50: 19000000 INFO @ Mon, 29 Jun 2020 22:52:53: 24000000 INFO @ Mon, 29 Jun 2020 22:52:53: 15000000 INFO @ Mon, 29 Jun 2020 22:52:57: 20000000 INFO @ Mon, 29 Jun 2020 22:53:00: 16000000 INFO @ Mon, 29 Jun 2020 22:53:00: 25000000 INFO @ Mon, 29 Jun 2020 22:53:04: 21000000 INFO @ Mon, 29 Jun 2020 22:53:07: 17000000 INFO @ Mon, 29 Jun 2020 22:53:07: 26000000 INFO @ Mon, 29 Jun 2020 22:53:11: 22000000 INFO @ Mon, 29 Jun 2020 22:53:14: 18000000 INFO @ Mon, 29 Jun 2020 22:53:15: 27000000 INFO @ Mon, 29 Jun 2020 22:53:19: 23000000 INFO @ Mon, 29 Jun 2020 22:53:21: 19000000 INFO @ Mon, 29 Jun 2020 22:53:22: 28000000 INFO @ Mon, 29 Jun 2020 22:53:26: 24000000 INFO @ Mon, 29 Jun 2020 22:53:28: 20000000 INFO @ Mon, 29 Jun 2020 22:53:29: 29000000 INFO @ Mon, 29 Jun 2020 22:53:34: 25000000 INFO @ Mon, 29 Jun 2020 22:53:35: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 22:53:35: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 22:53:35: #1 total tags in treatment: 29891072 INFO @ Mon, 29 Jun 2020 22:53:35: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 22:53:35: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 22:53:36: 21000000 INFO @ Mon, 29 Jun 2020 22:53:36: #1 tags after filtering in treatment: 29891072 INFO @ Mon, 29 Jun 2020 22:53:36: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 22:53:36: #1 finished! INFO @ Mon, 29 Jun 2020 22:53:36: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 22:53:36: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 22:53:38: #2 number of paired peaks: 68 WARNING @ Mon, 29 Jun 2020 22:53:38: Too few paired peaks (68) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 22:53:38: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX1433359/SRX1433359.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX1433359/SRX1433359.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX1433359/SRX1433359.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX1433359/SRX1433359.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 22:53:40: 26000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Mon, 29 Jun 2020 22:53:43: 22000000 INFO @ Mon, 29 Jun 2020 22:53:47: 27000000 INFO @ Mon, 29 Jun 2020 22:53:50: 23000000 INFO @ Mon, 29 Jun 2020 22:53:54: 28000000 INFO @ Mon, 29 Jun 2020 22:53:57: 24000000 INFO @ Mon, 29 Jun 2020 22:54:00: 29000000 INFO @ Mon, 29 Jun 2020 22:54:04: 25000000 INFO @ Mon, 29 Jun 2020 22:54:06: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 22:54:06: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 22:54:06: #1 total tags in treatment: 29891072 INFO @ Mon, 29 Jun 2020 22:54:06: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 22:54:06: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 22:54:07: #1 tags after filtering in treatment: 29891072 INFO @ Mon, 29 Jun 2020 22:54:07: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 22:54:07: #1 finished! INFO @ Mon, 29 Jun 2020 22:54:07: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 22:54:07: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 22:54:09: #2 number of paired peaks: 68 WARNING @ Mon, 29 Jun 2020 22:54:09: Too few paired peaks (68) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 22:54:09: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX1433359/SRX1433359.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX1433359/SRX1433359.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX1433359/SRX1433359.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX1433359/SRX1433359.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 22:54:11: 26000000 INFO @ Mon, 29 Jun 2020 22:54:18: 27000000 INFO @ Mon, 29 Jun 2020 22:54:25: 28000000 INFO @ Mon, 29 Jun 2020 22:54:32: 29000000 INFO @ Mon, 29 Jun 2020 22:54:38: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 22:54:38: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 22:54:38: #1 total tags in treatment: 29891072 INFO @ Mon, 29 Jun 2020 22:54:38: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 22:54:38: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 22:54:39: #1 tags after filtering in treatment: 29891072 INFO @ Mon, 29 Jun 2020 22:54:39: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 22:54:39: #1 finished! INFO @ Mon, 29 Jun 2020 22:54:39: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 22:54:39: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 22:54:41: #2 number of paired peaks: 68 WARNING @ Mon, 29 Jun 2020 22:54:41: Too few paired peaks (68) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 22:54:41: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX1433359/SRX1433359.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX1433359/SRX1433359.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX1433359/SRX1433359.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX1433359/SRX1433359.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。