Job ID = 16439640 SRX = SRX13572057 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 11129675 spots for SRR17399988/SRR17399988.sra Written 11129675 spots for SRR17399988/SRR17399988.sra fastq に変換しました。 bowtie でマッピング中... Your job 16439681 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:18 11129675 reads; of these: 11129675 (100.00%) were unpaired; of these: 533683 (4.80%) aligned 0 times 8824481 (79.29%) aligned exactly 1 time 1771511 (15.92%) aligned >1 times 95.20% overall alignment rate Time searching: 00:03:18 Overall time: 00:03:18 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 437789 / 10595992 = 0.0413 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 15:53:25: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX13572057/SRX13572057.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX13572057/SRX13572057.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX13572057/SRX13572057.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX13572057/SRX13572057.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 15:53:25: #1 read tag files... INFO @ Tue, 02 Aug 2022 15:53:25: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 15:53:31: 1000000 INFO @ Tue, 02 Aug 2022 15:53:37: 2000000 INFO @ Tue, 02 Aug 2022 15:53:43: 3000000 INFO @ Tue, 02 Aug 2022 15:53:49: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 15:53:54: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX13572057/SRX13572057.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX13572057/SRX13572057.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX13572057/SRX13572057.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX13572057/SRX13572057.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 15:53:54: #1 read tag files... INFO @ Tue, 02 Aug 2022 15:53:54: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 15:53:55: 5000000 INFO @ Tue, 02 Aug 2022 15:54:01: 1000000 INFO @ Tue, 02 Aug 2022 15:54:01: 6000000 INFO @ Tue, 02 Aug 2022 15:54:07: 2000000 INFO @ Tue, 02 Aug 2022 15:54:07: 7000000 INFO @ Tue, 02 Aug 2022 15:54:14: 8000000 INFO @ Tue, 02 Aug 2022 15:54:14: 3000000 INFO @ Tue, 02 Aug 2022 15:54:20: 9000000 INFO @ Tue, 02 Aug 2022 15:54:20: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 15:54:24: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX13572057/SRX13572057.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX13572057/SRX13572057.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX13572057/SRX13572057.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX13572057/SRX13572057.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 15:54:24: #1 read tag files... INFO @ Tue, 02 Aug 2022 15:54:24: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 15:54:27: 10000000 INFO @ Tue, 02 Aug 2022 15:54:27: 5000000 INFO @ Tue, 02 Aug 2022 15:54:28: #1 tag size is determined as 49 bps INFO @ Tue, 02 Aug 2022 15:54:28: #1 tag size = 49 INFO @ Tue, 02 Aug 2022 15:54:28: #1 total tags in treatment: 10158203 INFO @ Tue, 02 Aug 2022 15:54:28: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 15:54:28: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 15:54:28: #1 tags after filtering in treatment: 10158203 INFO @ Tue, 02 Aug 2022 15:54:28: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 15:54:28: #1 finished! INFO @ Tue, 02 Aug 2022 15:54:28: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 15:54:28: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 15:54:29: #2 number of paired peaks: 23 WARNING @ Tue, 02 Aug 2022 15:54:29: Too few paired peaks (23) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 15:54:29: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX13572057/SRX13572057.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13572057/SRX13572057.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13572057/SRX13572057.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13572057/SRX13572057.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 15:54:31: 1000000 INFO @ Tue, 02 Aug 2022 15:54:34: 6000000 INFO @ Tue, 02 Aug 2022 15:54:37: 2000000 INFO @ Tue, 02 Aug 2022 15:54:40: 7000000 INFO @ Tue, 02 Aug 2022 15:54:44: 3000000 INFO @ Tue, 02 Aug 2022 15:54:47: 8000000 INFO @ Tue, 02 Aug 2022 15:54:51: 4000000 INFO @ Tue, 02 Aug 2022 15:54:54: 9000000 INFO @ Tue, 02 Aug 2022 15:54:58: 5000000 INFO @ Tue, 02 Aug 2022 15:55:00: 10000000 INFO @ Tue, 02 Aug 2022 15:55:01: #1 tag size is determined as 49 bps INFO @ Tue, 02 Aug 2022 15:55:01: #1 tag size = 49 INFO @ Tue, 02 Aug 2022 15:55:01: #1 total tags in treatment: 10158203 INFO @ Tue, 02 Aug 2022 15:55:01: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 15:55:01: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 15:55:01: #1 tags after filtering in treatment: 10158203 INFO @ Tue, 02 Aug 2022 15:55:01: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 15:55:01: #1 finished! INFO @ Tue, 02 Aug 2022 15:55:01: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 15:55:01: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 15:55:02: #2 number of paired peaks: 23 WARNING @ Tue, 02 Aug 2022 15:55:02: Too few paired peaks (23) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 15:55:02: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX13572057/SRX13572057.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13572057/SRX13572057.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13572057/SRX13572057.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13572057/SRX13572057.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 15:55:05: 6000000 INFO @ Tue, 02 Aug 2022 15:55:11: 7000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 02 Aug 2022 15:55:17: 8000000 INFO @ Tue, 02 Aug 2022 15:55:23: 9000000 INFO @ Tue, 02 Aug 2022 15:55:29: 10000000 INFO @ Tue, 02 Aug 2022 15:55:30: #1 tag size is determined as 49 bps INFO @ Tue, 02 Aug 2022 15:55:30: #1 tag size = 49 INFO @ Tue, 02 Aug 2022 15:55:30: #1 total tags in treatment: 10158203 INFO @ Tue, 02 Aug 2022 15:55:30: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 15:55:30: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 15:55:30: #1 tags after filtering in treatment: 10158203 INFO @ Tue, 02 Aug 2022 15:55:30: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 15:55:30: #1 finished! INFO @ Tue, 02 Aug 2022 15:55:30: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 15:55:30: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 15:55:31: #2 number of paired peaks: 23 WARNING @ Tue, 02 Aug 2022 15:55:31: Too few paired peaks (23) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 15:55:31: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX13572057/SRX13572057.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13572057/SRX13572057.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13572057/SRX13572057.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13572057/SRX13572057.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。