Job ID = 16439634 SRX = SRX13572052 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 10683736 spots for SRR17399992/SRR17399992.sra Written 10683736 spots for SRR17399992/SRR17399992.sra fastq に変換しました。 bowtie でマッピング中... Your job 16439675 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:01 Multiseed full-index search: 00:03:17 10683736 reads; of these: 10683736 (100.00%) were unpaired; of these: 492935 (4.61%) aligned 0 times 8645293 (80.92%) aligned exactly 1 time 1545508 (14.47%) aligned >1 times 95.39% overall alignment rate Time searching: 00:03:18 Overall time: 00:03:18 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 827462 / 10190801 = 0.0812 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 15:53:22: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX13572052/SRX13572052.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX13572052/SRX13572052.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX13572052/SRX13572052.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX13572052/SRX13572052.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 15:53:22: #1 read tag files... INFO @ Tue, 02 Aug 2022 15:53:22: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 15:53:32: 1000000 INFO @ Tue, 02 Aug 2022 15:53:42: 2000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 15:53:52: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX13572052/SRX13572052.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX13572052/SRX13572052.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX13572052/SRX13572052.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX13572052/SRX13572052.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 15:53:52: #1 read tag files... INFO @ Tue, 02 Aug 2022 15:53:52: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 15:53:52: 3000000 INFO @ Tue, 02 Aug 2022 15:54:03: 4000000 INFO @ Tue, 02 Aug 2022 15:54:03: 1000000 INFO @ Tue, 02 Aug 2022 15:54:13: 5000000 INFO @ Tue, 02 Aug 2022 15:54:13: 2000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 15:54:22: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX13572052/SRX13572052.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX13572052/SRX13572052.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX13572052/SRX13572052.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX13572052/SRX13572052.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 15:54:22: #1 read tag files... INFO @ Tue, 02 Aug 2022 15:54:22: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 15:54:24: 6000000 INFO @ Tue, 02 Aug 2022 15:54:24: 3000000 INFO @ Tue, 02 Aug 2022 15:54:34: 1000000 INFO @ Tue, 02 Aug 2022 15:54:34: 4000000 INFO @ Tue, 02 Aug 2022 15:54:34: 7000000 INFO @ Tue, 02 Aug 2022 15:54:45: 5000000 INFO @ Tue, 02 Aug 2022 15:54:45: 2000000 INFO @ Tue, 02 Aug 2022 15:54:45: 8000000 INFO @ Tue, 02 Aug 2022 15:54:56: 6000000 INFO @ Tue, 02 Aug 2022 15:54:56: 9000000 INFO @ Tue, 02 Aug 2022 15:54:57: 3000000 INFO @ Tue, 02 Aug 2022 15:55:00: #1 tag size is determined as 49 bps INFO @ Tue, 02 Aug 2022 15:55:00: #1 tag size = 49 INFO @ Tue, 02 Aug 2022 15:55:00: #1 total tags in treatment: 9363339 INFO @ Tue, 02 Aug 2022 15:55:00: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 15:55:00: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 15:55:01: #1 tags after filtering in treatment: 9363339 INFO @ Tue, 02 Aug 2022 15:55:01: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 15:55:01: #1 finished! INFO @ Tue, 02 Aug 2022 15:55:01: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 15:55:01: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 15:55:01: #2 number of paired peaks: 92 WARNING @ Tue, 02 Aug 2022 15:55:01: Too few paired peaks (92) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 15:55:01: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX13572052/SRX13572052.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13572052/SRX13572052.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13572052/SRX13572052.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13572052/SRX13572052.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 15:55:06: 7000000 INFO @ Tue, 02 Aug 2022 15:55:09: 4000000 INFO @ Tue, 02 Aug 2022 15:55:17: 8000000 INFO @ Tue, 02 Aug 2022 15:55:19: 5000000 INFO @ Tue, 02 Aug 2022 15:55:28: 9000000 INFO @ Tue, 02 Aug 2022 15:55:29: 6000000 INFO @ Tue, 02 Aug 2022 15:55:32: #1 tag size is determined as 49 bps INFO @ Tue, 02 Aug 2022 15:55:32: #1 tag size = 49 INFO @ Tue, 02 Aug 2022 15:55:32: #1 total tags in treatment: 9363339 INFO @ Tue, 02 Aug 2022 15:55:32: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 15:55:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 02 Aug 2022 15:55:32: #1 tags after filtering in treatment: 9363339 INFO @ Tue, 02 Aug 2022 15:55:32: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 15:55:32: #1 finished! INFO @ Tue, 02 Aug 2022 15:55:32: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 15:55:32: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 15:55:33: #2 number of paired peaks: 92 WARNING @ Tue, 02 Aug 2022 15:55:33: Too few paired peaks (92) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 15:55:33: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX13572052/SRX13572052.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13572052/SRX13572052.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13572052/SRX13572052.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13572052/SRX13572052.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 15:55:40: 7000000 INFO @ Tue, 02 Aug 2022 15:55:50: 8000000 INFO @ Tue, 02 Aug 2022 15:56:00: 9000000 INFO @ Tue, 02 Aug 2022 15:56:04: #1 tag size is determined as 49 bps INFO @ Tue, 02 Aug 2022 15:56:04: #1 tag size = 49 INFO @ Tue, 02 Aug 2022 15:56:04: #1 total tags in treatment: 9363339 INFO @ Tue, 02 Aug 2022 15:56:04: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 15:56:04: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 15:56:04: #1 tags after filtering in treatment: 9363339 INFO @ Tue, 02 Aug 2022 15:56:04: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 15:56:04: #1 finished! INFO @ Tue, 02 Aug 2022 15:56:04: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 15:56:04: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 15:56:05: #2 number of paired peaks: 92 WARNING @ Tue, 02 Aug 2022 15:56:05: Too few paired peaks (92) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 15:56:05: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX13572052/SRX13572052.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13572052/SRX13572052.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13572052/SRX13572052.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13572052/SRX13572052.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。