Job ID = 16439633 SRX = SRX13572051 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 7992058 spots for SRR17399993/SRR17399993.sra Written 7992058 spots for SRR17399993/SRR17399993.sra fastq に変換しました。 bowtie でマッピング中... Your job 16439666 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:33 7992058 reads; of these: 7992058 (100.00%) were unpaired; of these: 349995 (4.38%) aligned 0 times 6244303 (78.13%) aligned exactly 1 time 1397760 (17.49%) aligned >1 times 95.62% overall alignment rate Time searching: 00:02:33 Overall time: 00:02:33 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_rmdupse_core] 356690 / 7642063 = 0.0467 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 15:51:26: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX13572051/SRX13572051.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX13572051/SRX13572051.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX13572051/SRX13572051.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX13572051/SRX13572051.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 15:51:26: #1 read tag files... INFO @ Tue, 02 Aug 2022 15:51:26: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 15:51:33: 1000000 INFO @ Tue, 02 Aug 2022 15:51:40: 2000000 INFO @ Tue, 02 Aug 2022 15:51:46: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 15:51:53: 4000000 INFO @ Tue, 02 Aug 2022 15:51:55: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX13572051/SRX13572051.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX13572051/SRX13572051.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX13572051/SRX13572051.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX13572051/SRX13572051.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 15:51:55: #1 read tag files... INFO @ Tue, 02 Aug 2022 15:51:55: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 15:52:00: 5000000 INFO @ Tue, 02 Aug 2022 15:52:02: 1000000 INFO @ Tue, 02 Aug 2022 15:52:07: 6000000 INFO @ Tue, 02 Aug 2022 15:52:09: 2000000 INFO @ Tue, 02 Aug 2022 15:52:14: 7000000 INFO @ Tue, 02 Aug 2022 15:52:16: #1 tag size is determined as 49 bps INFO @ Tue, 02 Aug 2022 15:52:16: #1 tag size = 49 INFO @ Tue, 02 Aug 2022 15:52:16: #1 total tags in treatment: 7285373 INFO @ Tue, 02 Aug 2022 15:52:16: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 15:52:16: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 15:52:16: #1 tags after filtering in treatment: 7285373 INFO @ Tue, 02 Aug 2022 15:52:16: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 15:52:16: #1 finished! INFO @ Tue, 02 Aug 2022 15:52:16: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 15:52:16: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 15:52:16: 3000000 INFO @ Tue, 02 Aug 2022 15:52:17: #2 number of paired peaks: 62 WARNING @ Tue, 02 Aug 2022 15:52:17: Too few paired peaks (62) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 15:52:17: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX13572051/SRX13572051.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 8 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13572051/SRX13572051.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13572051/SRX13572051.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13572051/SRX13572051.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 15:52:23: 4000000 INFO @ Tue, 02 Aug 2022 15:52:25: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX13572051/SRX13572051.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX13572051/SRX13572051.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX13572051/SRX13572051.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX13572051/SRX13572051.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 15:52:25: #1 read tag files... INFO @ Tue, 02 Aug 2022 15:52:25: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 15:52:30: 5000000 INFO @ Tue, 02 Aug 2022 15:52:32: 1000000 INFO @ Tue, 02 Aug 2022 15:52:37: 6000000 INFO @ Tue, 02 Aug 2022 15:52:39: 2000000 INFO @ Tue, 02 Aug 2022 15:52:44: 7000000 INFO @ Tue, 02 Aug 2022 15:52:46: #1 tag size is determined as 49 bps INFO @ Tue, 02 Aug 2022 15:52:46: #1 tag size = 49 INFO @ Tue, 02 Aug 2022 15:52:46: #1 total tags in treatment: 7285373 INFO @ Tue, 02 Aug 2022 15:52:46: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 15:52:46: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 15:52:46: #1 tags after filtering in treatment: 7285373 INFO @ Tue, 02 Aug 2022 15:52:46: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 15:52:46: #1 finished! INFO @ Tue, 02 Aug 2022 15:52:46: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 15:52:46: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 15:52:46: 3000000 INFO @ Tue, 02 Aug 2022 15:52:46: #2 number of paired peaks: 62 WARNING @ Tue, 02 Aug 2022 15:52:46: Too few paired peaks (62) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 15:52:46: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX13572051/SRX13572051.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13572051/SRX13572051.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13572051/SRX13572051.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13572051/SRX13572051.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 15:52:53: 4000000 INFO @ Tue, 02 Aug 2022 15:52:59: 5000000 INFO @ Tue, 02 Aug 2022 15:53:06: 6000000 INFO @ Tue, 02 Aug 2022 15:53:12: 7000000 INFO @ Tue, 02 Aug 2022 15:53:14: #1 tag size is determined as 49 bps INFO @ Tue, 02 Aug 2022 15:53:14: #1 tag size = 49 INFO @ Tue, 02 Aug 2022 15:53:14: #1 total tags in treatment: 7285373 INFO @ Tue, 02 Aug 2022 15:53:14: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 15:53:14: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 15:53:14: #1 tags after filtering in treatment: 7285373 INFO @ Tue, 02 Aug 2022 15:53:14: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 15:53:14: #1 finished! INFO @ Tue, 02 Aug 2022 15:53:14: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 15:53:14: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 15:53:14: #2 number of paired peaks: 62 WARNING @ Tue, 02 Aug 2022 15:53:14: Too few paired peaks (62) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 15:53:14: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX13572051/SRX13572051.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13572051/SRX13572051.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13572051/SRX13572051.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13572051/SRX13572051.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。