Job ID = 16439630 SRX = SRX13572048 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 14311616 spots for SRR17399997/SRR17399997.sra Written 14311616 spots for SRR17399997/SRR17399997.sra fastq に変換しました。 bowtie でマッピング中... Your job 16439673 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:57 14311616 reads; of these: 14311616 (100.00%) were unpaired; of these: 693309 (4.84%) aligned 0 times 11559184 (80.77%) aligned exactly 1 time 2059123 (14.39%) aligned >1 times 95.16% overall alignment rate Time searching: 00:03:57 Overall time: 00:03:57 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 709359 / 13618307 = 0.0521 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 15:53:59: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX13572048/SRX13572048.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX13572048/SRX13572048.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX13572048/SRX13572048.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX13572048/SRX13572048.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 15:53:59: #1 read tag files... INFO @ Tue, 02 Aug 2022 15:53:59: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 15:54:06: 1000000 INFO @ Tue, 02 Aug 2022 15:54:12: 2000000 INFO @ Tue, 02 Aug 2022 15:54:19: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 15:54:26: 4000000 INFO @ Tue, 02 Aug 2022 15:54:28: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX13572048/SRX13572048.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX13572048/SRX13572048.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX13572048/SRX13572048.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX13572048/SRX13572048.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 15:54:28: #1 read tag files... INFO @ Tue, 02 Aug 2022 15:54:28: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 15:54:33: 5000000 INFO @ Tue, 02 Aug 2022 15:54:35: 1000000 INFO @ Tue, 02 Aug 2022 15:54:40: 6000000 INFO @ Tue, 02 Aug 2022 15:54:42: 2000000 INFO @ Tue, 02 Aug 2022 15:54:47: 7000000 INFO @ Tue, 02 Aug 2022 15:54:49: 3000000 INFO @ Tue, 02 Aug 2022 15:54:55: 8000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 15:54:55: 4000000 INFO @ Tue, 02 Aug 2022 15:54:58: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX13572048/SRX13572048.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX13572048/SRX13572048.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX13572048/SRX13572048.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX13572048/SRX13572048.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 15:54:58: #1 read tag files... INFO @ Tue, 02 Aug 2022 15:54:58: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 15:55:02: 9000000 INFO @ Tue, 02 Aug 2022 15:55:02: 5000000 INFO @ Tue, 02 Aug 2022 15:55:03: 1000000 INFO @ Tue, 02 Aug 2022 15:55:08: 2000000 INFO @ Tue, 02 Aug 2022 15:55:09: 6000000 INFO @ Tue, 02 Aug 2022 15:55:10: 10000000 INFO @ Tue, 02 Aug 2022 15:55:13: 3000000 INFO @ Tue, 02 Aug 2022 15:55:17: 7000000 INFO @ Tue, 02 Aug 2022 15:55:17: 11000000 INFO @ Tue, 02 Aug 2022 15:55:18: 4000000 INFO @ Tue, 02 Aug 2022 15:55:23: 5000000 INFO @ Tue, 02 Aug 2022 15:55:24: 12000000 INFO @ Tue, 02 Aug 2022 15:55:24: 8000000 INFO @ Tue, 02 Aug 2022 15:55:28: 6000000 INFO @ Tue, 02 Aug 2022 15:55:30: #1 tag size is determined as 49 bps INFO @ Tue, 02 Aug 2022 15:55:30: #1 tag size = 49 INFO @ Tue, 02 Aug 2022 15:55:30: #1 total tags in treatment: 12908948 INFO @ Tue, 02 Aug 2022 15:55:30: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 15:55:30: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 15:55:30: #1 tags after filtering in treatment: 12908948 INFO @ Tue, 02 Aug 2022 15:55:30: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 15:55:30: #1 finished! INFO @ Tue, 02 Aug 2022 15:55:30: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 15:55:30: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 15:55:31: 9000000 INFO @ Tue, 02 Aug 2022 15:55:31: #2 number of paired peaks: 41 WARNING @ Tue, 02 Aug 2022 15:55:31: Too few paired peaks (41) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 15:55:31: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX13572048/SRX13572048.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13572048/SRX13572048.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13572048/SRX13572048.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13572048/SRX13572048.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 15:55:33: 7000000 INFO @ Tue, 02 Aug 2022 15:55:38: 10000000 INFO @ Tue, 02 Aug 2022 15:55:38: 8000000 INFO @ Tue, 02 Aug 2022 15:55:44: 9000000 INFO @ Tue, 02 Aug 2022 15:55:45: 11000000 INFO @ Tue, 02 Aug 2022 15:55:49: 10000000 INFO @ Tue, 02 Aug 2022 15:55:51: 12000000 INFO @ Tue, 02 Aug 2022 15:55:54: 11000000 INFO @ Tue, 02 Aug 2022 15:55:58: #1 tag size is determined as 49 bps INFO @ Tue, 02 Aug 2022 15:55:58: #1 tag size = 49 INFO @ Tue, 02 Aug 2022 15:55:58: #1 total tags in treatment: 12908948 INFO @ Tue, 02 Aug 2022 15:55:58: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 15:55:58: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 15:55:58: #1 tags after filtering in treatment: 12908948 INFO @ Tue, 02 Aug 2022 15:55:58: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 15:55:58: #1 finished! INFO @ Tue, 02 Aug 2022 15:55:58: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 15:55:58: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 15:55:59: 12000000 INFO @ Tue, 02 Aug 2022 15:55:59: #2 number of paired peaks: 41 WARNING @ Tue, 02 Aug 2022 15:55:59: Too few paired peaks (41) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 15:55:59: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX13572048/SRX13572048.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13572048/SRX13572048.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13572048/SRX13572048.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13572048/SRX13572048.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 15:56:03: #1 tag size is determined as 49 bps INFO @ Tue, 02 Aug 2022 15:56:03: #1 tag size = 49 INFO @ Tue, 02 Aug 2022 15:56:03: #1 total tags in treatment: 12908948 INFO @ Tue, 02 Aug 2022 15:56:03: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 15:56:03: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 15:56:04: #1 tags after filtering in treatment: 12908948 INFO @ Tue, 02 Aug 2022 15:56:04: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 15:56:04: #1 finished! INFO @ Tue, 02 Aug 2022 15:56:04: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 15:56:04: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 15:56:04: #2 number of paired peaks: 41 WARNING @ Tue, 02 Aug 2022 15:56:04: Too few paired peaks (41) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 15:56:04: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX13572048/SRX13572048.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13572048/SRX13572048.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13572048/SRX13572048.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13572048/SRX13572048.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。