Job ID = 16439626 SRX = SRX13572047 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 17703437 spots for SRR17399998/SRR17399998.sra Written 17703437 spots for SRR17399998/SRR17399998.sra fastq に変換しました。 bowtie でマッピング中... Your job 16439679 ("srTdm6") has been submitted Time loading reference: 00:00:01 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:49 17703437 reads; of these: 17703437 (100.00%) were unpaired; of these: 1313404 (7.42%) aligned 0 times 13940040 (78.74%) aligned exactly 1 time 2449993 (13.84%) aligned >1 times 92.58% overall alignment rate Time searching: 00:04:50 Overall time: 00:04:50 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 976381 / 16390033 = 0.0596 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 15:55:18: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX13572047/SRX13572047.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX13572047/SRX13572047.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX13572047/SRX13572047.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX13572047/SRX13572047.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 15:55:18: #1 read tag files... INFO @ Tue, 02 Aug 2022 15:55:18: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 15:55:25: 1000000 INFO @ Tue, 02 Aug 2022 15:55:32: 2000000 INFO @ Tue, 02 Aug 2022 15:55:38: 3000000 INFO @ Tue, 02 Aug 2022 15:55:44: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 15:55:47: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX13572047/SRX13572047.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX13572047/SRX13572047.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX13572047/SRX13572047.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX13572047/SRX13572047.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 15:55:47: #1 read tag files... INFO @ Tue, 02 Aug 2022 15:55:47: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 15:55:51: 5000000 INFO @ Tue, 02 Aug 2022 15:55:55: 1000000 INFO @ Tue, 02 Aug 2022 15:55:58: 6000000 INFO @ Tue, 02 Aug 2022 15:56:02: 2000000 INFO @ Tue, 02 Aug 2022 15:56:04: 7000000 INFO @ Tue, 02 Aug 2022 15:56:10: 3000000 INFO @ Tue, 02 Aug 2022 15:56:11: 8000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 15:56:17: 4000000 INFO @ Tue, 02 Aug 2022 15:56:17: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX13572047/SRX13572047.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX13572047/SRX13572047.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX13572047/SRX13572047.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX13572047/SRX13572047.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 15:56:17: #1 read tag files... INFO @ Tue, 02 Aug 2022 15:56:17: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 15:56:17: 9000000 INFO @ Tue, 02 Aug 2022 15:56:24: 5000000 INFO @ Tue, 02 Aug 2022 15:56:24: 1000000 INFO @ Tue, 02 Aug 2022 15:56:24: 10000000 INFO @ Tue, 02 Aug 2022 15:56:30: 6000000 INFO @ Tue, 02 Aug 2022 15:56:31: 2000000 INFO @ Tue, 02 Aug 2022 15:56:31: 11000000 INFO @ Tue, 02 Aug 2022 15:56:37: 3000000 INFO @ Tue, 02 Aug 2022 15:56:37: 7000000 INFO @ Tue, 02 Aug 2022 15:56:38: 12000000 INFO @ Tue, 02 Aug 2022 15:56:44: 4000000 INFO @ Tue, 02 Aug 2022 15:56:44: 8000000 INFO @ Tue, 02 Aug 2022 15:56:45: 13000000 INFO @ Tue, 02 Aug 2022 15:56:51: 5000000 INFO @ Tue, 02 Aug 2022 15:56:51: 9000000 INFO @ Tue, 02 Aug 2022 15:56:51: 14000000 INFO @ Tue, 02 Aug 2022 15:56:57: 6000000 INFO @ Tue, 02 Aug 2022 15:56:58: 10000000 INFO @ Tue, 02 Aug 2022 15:56:58: 15000000 INFO @ Tue, 02 Aug 2022 15:57:01: #1 tag size is determined as 49 bps INFO @ Tue, 02 Aug 2022 15:57:01: #1 tag size = 49 INFO @ Tue, 02 Aug 2022 15:57:01: #1 total tags in treatment: 15413652 INFO @ Tue, 02 Aug 2022 15:57:01: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 15:57:01: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 15:57:01: #1 tags after filtering in treatment: 15413652 INFO @ Tue, 02 Aug 2022 15:57:01: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 15:57:01: #1 finished! INFO @ Tue, 02 Aug 2022 15:57:01: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 15:57:01: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 15:57:02: #2 number of paired peaks: 37 WARNING @ Tue, 02 Aug 2022 15:57:02: Too few paired peaks (37) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 15:57:02: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX13572047/SRX13572047.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13572047/SRX13572047.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13572047/SRX13572047.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13572047/SRX13572047.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 15:57:04: 7000000 INFO @ Tue, 02 Aug 2022 15:57:04: 11000000 INFO @ Tue, 02 Aug 2022 15:57:11: 8000000 INFO @ Tue, 02 Aug 2022 15:57:11: 12000000 INFO @ Tue, 02 Aug 2022 15:57:17: 13000000 INFO @ Tue, 02 Aug 2022 15:57:18: 9000000 INFO @ Tue, 02 Aug 2022 15:57:24: 14000000 INFO @ Tue, 02 Aug 2022 15:57:24: 10000000 INFO @ Tue, 02 Aug 2022 15:57:30: 15000000 INFO @ Tue, 02 Aug 2022 15:57:31: 11000000 INFO @ Tue, 02 Aug 2022 15:57:33: #1 tag size is determined as 49 bps INFO @ Tue, 02 Aug 2022 15:57:33: #1 tag size = 49 INFO @ Tue, 02 Aug 2022 15:57:33: #1 total tags in treatment: 15413652 INFO @ Tue, 02 Aug 2022 15:57:33: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 15:57:33: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 15:57:34: #1 tags after filtering in treatment: 15413652 INFO @ Tue, 02 Aug 2022 15:57:34: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 15:57:34: #1 finished! INFO @ Tue, 02 Aug 2022 15:57:34: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 15:57:34: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 15:57:35: #2 number of paired peaks: 37 WARNING @ Tue, 02 Aug 2022 15:57:35: Too few paired peaks (37) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 15:57:35: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX13572047/SRX13572047.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13572047/SRX13572047.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13572047/SRX13572047.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13572047/SRX13572047.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 15:57:37: 12000000 INFO @ Tue, 02 Aug 2022 15:57:44: 13000000 INFO @ Tue, 02 Aug 2022 15:57:50: 14000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 02 Aug 2022 15:57:57: 15000000 INFO @ Tue, 02 Aug 2022 15:57:59: #1 tag size is determined as 49 bps INFO @ Tue, 02 Aug 2022 15:57:59: #1 tag size = 49 INFO @ Tue, 02 Aug 2022 15:57:59: #1 total tags in treatment: 15413652 INFO @ Tue, 02 Aug 2022 15:57:59: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 15:57:59: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 15:58:00: #1 tags after filtering in treatment: 15413652 INFO @ Tue, 02 Aug 2022 15:58:00: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 15:58:00: #1 finished! INFO @ Tue, 02 Aug 2022 15:58:00: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 15:58:00: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 15:58:01: #2 number of paired peaks: 37 WARNING @ Tue, 02 Aug 2022 15:58:01: Too few paired peaks (37) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 15:58:01: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX13572047/SRX13572047.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13572047/SRX13572047.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13572047/SRX13572047.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13572047/SRX13572047.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。