Job ID = 16439625 SRX = SRX13572046 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 15685976 spots for SRR17399999/SRR17399999.sra Written 15685976 spots for SRR17399999/SRR17399999.sra fastq に変換しました。 bowtie でマッピング中... Your job 16439669 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:44 15685976 reads; of these: 15685976 (100.00%) were unpaired; of these: 1698656 (10.83%) aligned 0 times 12027671 (76.68%) aligned exactly 1 time 1959649 (12.49%) aligned >1 times 89.17% overall alignment rate Time searching: 00:03:44 Overall time: 00:03:44 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 784539 / 13987320 = 0.0561 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 15:52:13: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX13572046/SRX13572046.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX13572046/SRX13572046.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX13572046/SRX13572046.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX13572046/SRX13572046.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 15:52:13: #1 read tag files... INFO @ Tue, 02 Aug 2022 15:52:13: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 15:52:20: 1000000 INFO @ Tue, 02 Aug 2022 15:52:26: 2000000 INFO @ Tue, 02 Aug 2022 15:52:32: 3000000 INFO @ Tue, 02 Aug 2022 15:52:39: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 15:52:42: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX13572046/SRX13572046.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX13572046/SRX13572046.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX13572046/SRX13572046.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX13572046/SRX13572046.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 15:52:42: #1 read tag files... INFO @ Tue, 02 Aug 2022 15:52:42: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 15:52:45: 5000000 INFO @ Tue, 02 Aug 2022 15:52:48: 1000000 INFO @ Tue, 02 Aug 2022 15:52:51: 6000000 INFO @ Tue, 02 Aug 2022 15:52:54: 2000000 INFO @ Tue, 02 Aug 2022 15:52:57: 7000000 INFO @ Tue, 02 Aug 2022 15:53:00: 3000000 INFO @ Tue, 02 Aug 2022 15:53:04: 8000000 INFO @ Tue, 02 Aug 2022 15:53:05: 4000000 INFO @ Tue, 02 Aug 2022 15:53:10: 9000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 15:53:11: 5000000 INFO @ Tue, 02 Aug 2022 15:53:12: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX13572046/SRX13572046.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX13572046/SRX13572046.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX13572046/SRX13572046.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX13572046/SRX13572046.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 15:53:12: #1 read tag files... INFO @ Tue, 02 Aug 2022 15:53:12: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 15:53:16: 6000000 INFO @ Tue, 02 Aug 2022 15:53:16: 10000000 INFO @ Tue, 02 Aug 2022 15:53:19: 1000000 INFO @ Tue, 02 Aug 2022 15:53:22: 7000000 INFO @ Tue, 02 Aug 2022 15:53:23: 11000000 INFO @ Tue, 02 Aug 2022 15:53:25: 2000000 INFO @ Tue, 02 Aug 2022 15:53:28: 8000000 INFO @ Tue, 02 Aug 2022 15:53:29: 12000000 INFO @ Tue, 02 Aug 2022 15:53:32: 3000000 INFO @ Tue, 02 Aug 2022 15:53:33: 9000000 INFO @ Tue, 02 Aug 2022 15:53:36: 13000000 INFO @ Tue, 02 Aug 2022 15:53:37: #1 tag size is determined as 49 bps INFO @ Tue, 02 Aug 2022 15:53:37: #1 tag size = 49 INFO @ Tue, 02 Aug 2022 15:53:37: #1 total tags in treatment: 13202781 INFO @ Tue, 02 Aug 2022 15:53:37: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 15:53:37: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 15:53:37: #1 tags after filtering in treatment: 13202781 INFO @ Tue, 02 Aug 2022 15:53:37: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 15:53:37: #1 finished! INFO @ Tue, 02 Aug 2022 15:53:37: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 15:53:37: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 15:53:38: 4000000 INFO @ Tue, 02 Aug 2022 15:53:38: #2 number of paired peaks: 47 WARNING @ Tue, 02 Aug 2022 15:53:38: Too few paired peaks (47) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 15:53:38: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX13572046/SRX13572046.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13572046/SRX13572046.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13572046/SRX13572046.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13572046/SRX13572046.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 15:53:39: 10000000 INFO @ Tue, 02 Aug 2022 15:53:44: 5000000 INFO @ Tue, 02 Aug 2022 15:53:45: 11000000 INFO @ Tue, 02 Aug 2022 15:53:50: 12000000 INFO @ Tue, 02 Aug 2022 15:53:51: 6000000 INFO @ Tue, 02 Aug 2022 15:53:56: 13000000 INFO @ Tue, 02 Aug 2022 15:53:57: 7000000 INFO @ Tue, 02 Aug 2022 15:53:57: #1 tag size is determined as 49 bps INFO @ Tue, 02 Aug 2022 15:53:57: #1 tag size = 49 INFO @ Tue, 02 Aug 2022 15:53:57: #1 total tags in treatment: 13202781 INFO @ Tue, 02 Aug 2022 15:53:57: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 15:53:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 15:53:58: #1 tags after filtering in treatment: 13202781 INFO @ Tue, 02 Aug 2022 15:53:58: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 15:53:58: #1 finished! INFO @ Tue, 02 Aug 2022 15:53:58: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 15:53:58: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 15:53:58: #2 number of paired peaks: 47 WARNING @ Tue, 02 Aug 2022 15:53:58: Too few paired peaks (47) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 15:53:58: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX13572046/SRX13572046.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13572046/SRX13572046.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13572046/SRX13572046.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13572046/SRX13572046.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 15:54:03: 8000000 INFO @ Tue, 02 Aug 2022 15:54:09: 9000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 02 Aug 2022 15:54:15: 10000000 INFO @ Tue, 02 Aug 2022 15:54:21: 11000000 INFO @ Tue, 02 Aug 2022 15:54:27: 12000000 INFO @ Tue, 02 Aug 2022 15:54:32: 13000000 INFO @ Tue, 02 Aug 2022 15:54:34: #1 tag size is determined as 49 bps INFO @ Tue, 02 Aug 2022 15:54:34: #1 tag size = 49 INFO @ Tue, 02 Aug 2022 15:54:34: #1 total tags in treatment: 13202781 INFO @ Tue, 02 Aug 2022 15:54:34: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 15:54:34: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 15:54:34: #1 tags after filtering in treatment: 13202781 INFO @ Tue, 02 Aug 2022 15:54:34: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 15:54:34: #1 finished! INFO @ Tue, 02 Aug 2022 15:54:34: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 15:54:34: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 15:54:35: #2 number of paired peaks: 47 WARNING @ Tue, 02 Aug 2022 15:54:35: Too few paired peaks (47) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 15:54:35: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX13572046/SRX13572046.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13572046/SRX13572046.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13572046/SRX13572046.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13572046/SRX13572046.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。