Job ID = 1293890 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 120,708,385 reads read : 120,708,385 reads written : 120,708,385 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:42:41 120708385 reads; of these: 120708385 (100.00%) were unpaired; of these: 19660188 (16.29%) aligned 0 times 70234177 (58.19%) aligned exactly 1 time 30814020 (25.53%) aligned >1 times 83.71% overall alignment rate Time searching: 00:42:41 Overall time: 00:42:41 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 44 files... [bam_rmdupse_core] 53836792 / 101048197 = 0.5328 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 03 Jun 2019 04:15:11: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX135532/SRX135532.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX135532/SRX135532.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX135532/SRX135532.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX135532/SRX135532.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 04:15:11: #1 read tag files... INFO @ Mon, 03 Jun 2019 04:15:11: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 04:15:11: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX135532/SRX135532.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX135532/SRX135532.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX135532/SRX135532.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX135532/SRX135532.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 04:15:11: #1 read tag files... INFO @ Mon, 03 Jun 2019 04:15:11: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 04:15:11: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX135532/SRX135532.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX135532/SRX135532.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX135532/SRX135532.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX135532/SRX135532.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 04:15:11: #1 read tag files... INFO @ Mon, 03 Jun 2019 04:15:11: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 04:15:19: 1000000 INFO @ Mon, 03 Jun 2019 04:15:19: 1000000 INFO @ Mon, 03 Jun 2019 04:15:21: 1000000 INFO @ Mon, 03 Jun 2019 04:15:26: 2000000 INFO @ Mon, 03 Jun 2019 04:15:27: 2000000 INFO @ Mon, 03 Jun 2019 04:15:29: 2000000 INFO @ Mon, 03 Jun 2019 04:15:34: 3000000 INFO @ Mon, 03 Jun 2019 04:15:35: 3000000 INFO @ Mon, 03 Jun 2019 04:15:39: 3000000 INFO @ Mon, 03 Jun 2019 04:15:41: 4000000 INFO @ Mon, 03 Jun 2019 04:15:43: 4000000 INFO @ Mon, 03 Jun 2019 04:15:48: 4000000 INFO @ Mon, 03 Jun 2019 04:15:49: 5000000 INFO @ Mon, 03 Jun 2019 04:15:50: 5000000 INFO @ Mon, 03 Jun 2019 04:15:56: 6000000 INFO @ Mon, 03 Jun 2019 04:15:57: 5000000 INFO @ Mon, 03 Jun 2019 04:15:58: 6000000 INFO @ Mon, 03 Jun 2019 04:16:04: 7000000 INFO @ Mon, 03 Jun 2019 04:16:05: 6000000 INFO @ Mon, 03 Jun 2019 04:16:06: 7000000 INFO @ Mon, 03 Jun 2019 04:16:11: 8000000 INFO @ Mon, 03 Jun 2019 04:16:13: 8000000 INFO @ Mon, 03 Jun 2019 04:16:14: 7000000 INFO @ Mon, 03 Jun 2019 04:16:19: 9000000 INFO @ Mon, 03 Jun 2019 04:16:21: 9000000 INFO @ Mon, 03 Jun 2019 04:16:23: 8000000 INFO @ Mon, 03 Jun 2019 04:16:26: 10000000 INFO @ Mon, 03 Jun 2019 04:16:29: 10000000 INFO @ Mon, 03 Jun 2019 04:16:31: 9000000 INFO @ Mon, 03 Jun 2019 04:16:34: 11000000 INFO @ Mon, 03 Jun 2019 04:16:36: 11000000 INFO @ Mon, 03 Jun 2019 04:16:40: 10000000 INFO @ Mon, 03 Jun 2019 04:16:41: 12000000 INFO @ Mon, 03 Jun 2019 04:16:44: 12000000 INFO @ Mon, 03 Jun 2019 04:16:48: 13000000 INFO @ Mon, 03 Jun 2019 04:16:49: 11000000 INFO @ Mon, 03 Jun 2019 04:16:51: 13000000 INFO @ Mon, 03 Jun 2019 04:16:56: 14000000 INFO @ Mon, 03 Jun 2019 04:16:58: 12000000 INFO @ Mon, 03 Jun 2019 04:16:59: 14000000 INFO @ Mon, 03 Jun 2019 04:17:03: 15000000 INFO @ Mon, 03 Jun 2019 04:17:06: 13000000 INFO @ Mon, 03 Jun 2019 04:17:07: 15000000 INFO @ Mon, 03 Jun 2019 04:17:11: 16000000 INFO @ Mon, 03 Jun 2019 04:17:14: 16000000 INFO @ Mon, 03 Jun 2019 04:17:15: 14000000 INFO @ Mon, 03 Jun 2019 04:17:18: 17000000 INFO @ Mon, 03 Jun 2019 04:17:22: 17000000 INFO @ Mon, 03 Jun 2019 04:17:24: 15000000 INFO @ Mon, 03 Jun 2019 04:17:26: 18000000 INFO @ Mon, 03 Jun 2019 04:17:30: 18000000 INFO @ Mon, 03 Jun 2019 04:17:32: 16000000 INFO @ Mon, 03 Jun 2019 04:17:33: 19000000 INFO @ Mon, 03 Jun 2019 04:17:38: 19000000 INFO @ Mon, 03 Jun 2019 04:17:40: 20000000 INFO @ Mon, 03 Jun 2019 04:17:41: 17000000 INFO @ Mon, 03 Jun 2019 04:17:46: 20000000 INFO @ Mon, 03 Jun 2019 04:17:48: 21000000 INFO @ Mon, 03 Jun 2019 04:17:49: 18000000 INFO @ Mon, 03 Jun 2019 04:17:54: 21000000 INFO @ Mon, 03 Jun 2019 04:17:55: 22000000 INFO @ Mon, 03 Jun 2019 04:17:58: 19000000 INFO @ Mon, 03 Jun 2019 04:18:02: 23000000 INFO @ Mon, 03 Jun 2019 04:18:02: 22000000 INFO @ Mon, 03 Jun 2019 04:18:06: 20000000 INFO @ Mon, 03 Jun 2019 04:18:08: 24000000 INFO @ Mon, 03 Jun 2019 04:18:10: 23000000 INFO @ Mon, 03 Jun 2019 04:18:14: 21000000 INFO @ Mon, 03 Jun 2019 04:18:15: 25000000 INFO @ Mon, 03 Jun 2019 04:18:17: 24000000 INFO @ Mon, 03 Jun 2019 04:18:22: 26000000 INFO @ Mon, 03 Jun 2019 04:18:23: 22000000 INFO @ Mon, 03 Jun 2019 04:18:25: 25000000 INFO @ Mon, 03 Jun 2019 04:18:29: 27000000 INFO @ Mon, 03 Jun 2019 04:18:31: 23000000 INFO @ Mon, 03 Jun 2019 04:18:32: 26000000 INFO @ Mon, 03 Jun 2019 04:18:36: 28000000 INFO @ Mon, 03 Jun 2019 04:18:40: 27000000 INFO @ Mon, 03 Jun 2019 04:18:40: 24000000 INFO @ Mon, 03 Jun 2019 04:18:43: 29000000 INFO @ Mon, 03 Jun 2019 04:18:47: 28000000 INFO @ Mon, 03 Jun 2019 04:18:48: 25000000 INFO @ Mon, 03 Jun 2019 04:18:49: 30000000 INFO @ Mon, 03 Jun 2019 04:18:54: 29000000 INFO @ Mon, 03 Jun 2019 04:18:56: 31000000 INFO @ Mon, 03 Jun 2019 04:18:57: 26000000 INFO @ Mon, 03 Jun 2019 04:19:02: 30000000 INFO @ Mon, 03 Jun 2019 04:19:03: 32000000 INFO @ Mon, 03 Jun 2019 04:19:05: 27000000 INFO @ Mon, 03 Jun 2019 04:19:09: 31000000 INFO @ Mon, 03 Jun 2019 04:19:09: 33000000 INFO @ Mon, 03 Jun 2019 04:19:14: 28000000 INFO @ Mon, 03 Jun 2019 04:19:16: 32000000 INFO @ Mon, 03 Jun 2019 04:19:16: 34000000 INFO @ Mon, 03 Jun 2019 04:19:22: 29000000 INFO @ Mon, 03 Jun 2019 04:19:23: 35000000 INFO @ Mon, 03 Jun 2019 04:19:24: 33000000 INFO @ Mon, 03 Jun 2019 04:19:30: 36000000 INFO @ Mon, 03 Jun 2019 04:19:30: 30000000 INFO @ Mon, 03 Jun 2019 04:19:31: 34000000 INFO @ Mon, 03 Jun 2019 04:19:37: 37000000 INFO @ Mon, 03 Jun 2019 04:19:38: 31000000 INFO @ Mon, 03 Jun 2019 04:19:39: 35000000 INFO @ Mon, 03 Jun 2019 04:19:44: 38000000 INFO @ Mon, 03 Jun 2019 04:19:46: 36000000 INFO @ Mon, 03 Jun 2019 04:19:47: 32000000 INFO @ Mon, 03 Jun 2019 04:19:51: 39000000 INFO @ Mon, 03 Jun 2019 04:19:54: 37000000 INFO @ Mon, 03 Jun 2019 04:19:55: 33000000 INFO @ Mon, 03 Jun 2019 04:19:58: 40000000 INFO @ Mon, 03 Jun 2019 04:20:02: 38000000 INFO @ Mon, 03 Jun 2019 04:20:04: 34000000 INFO @ Mon, 03 Jun 2019 04:20:05: 41000000 INFO @ Mon, 03 Jun 2019 04:20:10: 39000000 INFO @ Mon, 03 Jun 2019 04:20:13: 42000000 INFO @ Mon, 03 Jun 2019 04:20:13: 35000000 INFO @ Mon, 03 Jun 2019 04:20:17: 40000000 INFO @ Mon, 03 Jun 2019 04:20:20: 43000000 INFO @ Mon, 03 Jun 2019 04:20:21: 36000000 INFO @ Mon, 03 Jun 2019 04:20:24: 41000000 INFO @ Mon, 03 Jun 2019 04:20:27: 44000000 INFO @ Mon, 03 Jun 2019 04:20:30: 37000000 INFO @ Mon, 03 Jun 2019 04:20:32: 42000000 INFO @ Mon, 03 Jun 2019 04:20:34: 45000000 INFO @ Mon, 03 Jun 2019 04:20:38: 38000000 INFO @ Mon, 03 Jun 2019 04:20:40: 43000000 INFO @ Mon, 03 Jun 2019 04:20:41: 46000000 INFO @ Mon, 03 Jun 2019 04:20:47: 39000000 INFO @ Mon, 03 Jun 2019 04:20:47: 47000000 INFO @ Mon, 03 Jun 2019 04:20:48: 44000000 INFO @ Mon, 03 Jun 2019 04:20:49: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 04:20:49: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 04:20:49: #1 total tags in treatment: 47211405 INFO @ Mon, 03 Jun 2019 04:20:49: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 04:20:49: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 04:20:50: #1 tags after filtering in treatment: 47211405 INFO @ Mon, 03 Jun 2019 04:20:50: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 04:20:50: #1 finished! INFO @ Mon, 03 Jun 2019 04:20:50: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 04:20:50: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 04:20:54: #2 number of paired peaks: 0 WARNING @ Mon, 03 Jun 2019 04:20:54: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 04:20:54: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX135532/SRX135532.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX135532/SRX135532.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX135532/SRX135532.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX135532/SRX135532.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 04:20:55: 40000000 INFO @ Mon, 03 Jun 2019 04:20:55: 45000000 INFO @ Mon, 03 Jun 2019 04:21:03: 46000000 INFO @ Mon, 03 Jun 2019 04:21:04: 41000000 INFO @ Mon, 03 Jun 2019 04:21:10: 47000000 INFO @ Mon, 03 Jun 2019 04:21:12: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 04:21:12: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 04:21:12: #1 total tags in treatment: 47211405 INFO @ Mon, 03 Jun 2019 04:21:12: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 04:21:12: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 04:21:13: 42000000 INFO @ Mon, 03 Jun 2019 04:21:13: #1 tags after filtering in treatment: 47211405 INFO @ Mon, 03 Jun 2019 04:21:13: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 04:21:13: #1 finished! INFO @ Mon, 03 Jun 2019 04:21:13: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 04:21:13: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 04:21:17: #2 number of paired peaks: 0 WARNING @ Mon, 03 Jun 2019 04:21:17: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 04:21:17: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX135532/SRX135532.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX135532/SRX135532.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX135532/SRX135532.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX135532/SRX135532.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 04:21:22: 43000000 INFO @ Mon, 03 Jun 2019 04:21:30: 44000000 INFO @ Mon, 03 Jun 2019 04:21:39: 45000000 INFO @ Mon, 03 Jun 2019 04:21:47: 46000000 INFO @ Mon, 03 Jun 2019 04:21:55: 47000000 INFO @ Mon, 03 Jun 2019 04:21:58: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 04:21:58: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 04:21:58: #1 total tags in treatment: 47211405 INFO @ Mon, 03 Jun 2019 04:21:58: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 04:21:58: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 04:21:59: #1 tags after filtering in treatment: 47211405 INFO @ Mon, 03 Jun 2019 04:21:59: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 04:21:59: #1 finished! INFO @ Mon, 03 Jun 2019 04:21:59: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 04:21:59: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 04:22:03: #2 number of paired peaks: 0 WARNING @ Mon, 03 Jun 2019 04:22:03: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 04:22:03: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX135532/SRX135532.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX135532/SRX135532.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX135532/SRX135532.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX135532/SRX135532.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。