Job ID = 9158459 sra ファイルのダウンロード中... Completed: 8244102K bytes transferred in 70 seconds (955947K bits/sec), in 2 files, 3 directories. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 68538619 spots for /home/okishinya/chipatlas/results/dm3/SRX1342245/SRR2672981.sra Written 68538619 spots total Written 215590594 spots for /home/okishinya/chipatlas/results/dm3/SRX1342245/SRR2672982.sra Written 215590594 spots total fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:01 Multiseed full-index search: 01:28:01 284129213 reads; of these: 284129213 (100.00%) were unpaired; of these: 70409552 (24.78%) aligned 0 times 167583128 (58.98%) aligned exactly 1 time 46136533 (16.24%) aligned >1 times 75.22% overall alignment rate Time searching: 01:28:02 Overall time: 01:28:02 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 88 files... [bam_rmdupse_core] 165510410 / 213719661 = 0.7744 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Tue, 27 Jun 2017 19:07:32: # Command line: callpeak -t SRX1342245.bam -f BAM -g dm -n SRX1342245.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX1342245.05 # format = BAM # ChIP-seq file = ['SRX1342245.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 19:07:32: #1 read tag files... INFO @ Tue, 27 Jun 2017 19:07:32: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 19:07:33: # Command line: callpeak -t SRX1342245.bam -f BAM -g dm -n SRX1342245.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX1342245.10 # format = BAM # ChIP-seq file = ['SRX1342245.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 19:07:33: #1 read tag files... INFO @ Tue, 27 Jun 2017 19:07:33: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 19:07:33: # Command line: callpeak -t SRX1342245.bam -f BAM -g dm -n SRX1342245.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX1342245.20 # format = BAM # ChIP-seq file = ['SRX1342245.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 19:07:33: #1 read tag files... INFO @ Tue, 27 Jun 2017 19:07:33: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 19:07:41: 1000000 INFO @ Tue, 27 Jun 2017 19:07:42: 1000000 INFO @ Tue, 27 Jun 2017 19:07:42: 1000000 INFO @ Tue, 27 Jun 2017 19:07:50: 2000000 INFO @ Tue, 27 Jun 2017 19:07:50: 2000000 INFO @ Tue, 27 Jun 2017 19:07:52: 2000000 INFO @ Tue, 27 Jun 2017 19:07:58: 3000000 INFO @ Tue, 27 Jun 2017 19:07:59: 3000000 INFO @ Tue, 27 Jun 2017 19:08:01: 3000000 INFO @ Tue, 27 Jun 2017 19:08:06: 4000000 INFO @ Tue, 27 Jun 2017 19:08:09: 4000000 INFO @ Tue, 27 Jun 2017 19:08:12: 4000000 INFO @ Tue, 27 Jun 2017 19:08:15: 5000000 INFO @ Tue, 27 Jun 2017 19:08:18: 5000000 INFO @ Tue, 27 Jun 2017 19:08:21: 5000000 INFO @ Tue, 27 Jun 2017 19:08:24: 6000000 INFO @ Tue, 27 Jun 2017 19:08:27: 6000000 INFO @ Tue, 27 Jun 2017 19:08:30: 6000000 INFO @ Tue, 27 Jun 2017 19:08:33: 7000000 INFO @ Tue, 27 Jun 2017 19:08:36: 7000000 INFO @ Tue, 27 Jun 2017 19:08:39: 7000000 INFO @ Tue, 27 Jun 2017 19:08:42: 8000000 INFO @ Tue, 27 Jun 2017 19:08:46: 8000000 INFO @ Tue, 27 Jun 2017 19:08:48: 8000000 INFO @ Tue, 27 Jun 2017 19:08:51: 9000000 INFO @ Tue, 27 Jun 2017 19:08:55: 9000000 INFO @ Tue, 27 Jun 2017 19:08:57: 9000000 INFO @ Tue, 27 Jun 2017 19:09:00: 10000000 INFO @ Tue, 27 Jun 2017 19:09:04: 10000000 INFO @ Tue, 27 Jun 2017 19:09:06: 10000000 INFO @ Tue, 27 Jun 2017 19:09:09: 11000000 INFO @ Tue, 27 Jun 2017 19:09:13: 11000000 INFO @ Tue, 27 Jun 2017 19:09:16: 11000000 INFO @ Tue, 27 Jun 2017 19:09:17: 12000000 INFO @ Tue, 27 Jun 2017 19:09:23: 12000000 INFO @ Tue, 27 Jun 2017 19:09:25: 13000000 INFO @ Tue, 27 Jun 2017 19:09:26: 12000000 INFO @ Tue, 27 Jun 2017 19:09:34: 14000000 INFO @ Tue, 27 Jun 2017 19:09:34: 13000000 INFO @ Tue, 27 Jun 2017 19:09:37: 13000000 INFO @ Tue, 27 Jun 2017 19:09:42: 15000000 INFO @ Tue, 27 Jun 2017 19:09:44: 14000000 INFO @ Tue, 27 Jun 2017 19:09:47: 14000000 INFO @ Tue, 27 Jun 2017 19:09:51: 16000000 INFO @ Tue, 27 Jun 2017 19:09:53: 15000000 INFO @ Tue, 27 Jun 2017 19:09:56: 15000000 INFO @ Tue, 27 Jun 2017 19:10:00: 17000000 INFO @ Tue, 27 Jun 2017 19:10:02: 16000000 INFO @ Tue, 27 Jun 2017 19:10:05: 16000000 INFO @ Tue, 27 Jun 2017 19:10:09: 18000000 INFO @ Tue, 27 Jun 2017 19:10:12: 17000000 INFO @ Tue, 27 Jun 2017 19:10:14: 17000000 INFO @ Tue, 27 Jun 2017 19:10:17: 19000000 INFO @ Tue, 27 Jun 2017 19:10:22: 18000000 INFO @ Tue, 27 Jun 2017 19:10:24: 18000000 INFO @ Tue, 27 Jun 2017 19:10:26: 20000000 INFO @ Tue, 27 Jun 2017 19:10:31: 19000000 INFO @ Tue, 27 Jun 2017 19:10:33: 19000000 INFO @ Tue, 27 Jun 2017 19:10:34: 21000000 INFO @ Tue, 27 Jun 2017 19:10:40: 20000000 INFO @ Tue, 27 Jun 2017 19:10:42: 20000000 INFO @ Tue, 27 Jun 2017 19:10:43: 22000000 INFO @ Tue, 27 Jun 2017 19:10:49: 21000000 INFO @ Tue, 27 Jun 2017 19:10:51: 21000000 INFO @ Tue, 27 Jun 2017 19:10:52: 23000000 INFO @ Tue, 27 Jun 2017 19:10:58: 22000000 INFO @ Tue, 27 Jun 2017 19:11:00: 22000000 INFO @ Tue, 27 Jun 2017 19:11:02: 24000000 INFO @ Tue, 27 Jun 2017 19:11:07: 23000000 INFO @ Tue, 27 Jun 2017 19:11:09: 23000000 INFO @ Tue, 27 Jun 2017 19:11:11: 25000000 INFO @ Tue, 27 Jun 2017 19:11:16: 24000000 INFO @ Tue, 27 Jun 2017 19:11:18: 24000000 INFO @ Tue, 27 Jun 2017 19:11:20: 26000000 INFO @ Tue, 27 Jun 2017 19:11:25: 25000000 INFO @ Tue, 27 Jun 2017 19:11:28: 25000000 INFO @ Tue, 27 Jun 2017 19:11:30: 27000000 INFO @ Tue, 27 Jun 2017 19:11:34: 26000000 INFO @ Tue, 27 Jun 2017 19:11:37: 26000000 INFO @ Tue, 27 Jun 2017 19:11:39: 28000000 INFO @ Tue, 27 Jun 2017 19:11:43: 27000000 INFO @ Tue, 27 Jun 2017 19:11:46: 27000000 INFO @ Tue, 27 Jun 2017 19:11:48: 29000000 INFO @ Tue, 27 Jun 2017 19:11:52: 28000000 INFO @ Tue, 27 Jun 2017 19:11:55: 28000000 INFO @ Tue, 27 Jun 2017 19:11:57: 30000000 INFO @ Tue, 27 Jun 2017 19:12:01: 29000000 INFO @ Tue, 27 Jun 2017 19:12:04: 29000000 INFO @ Tue, 27 Jun 2017 19:12:06: 31000000 INFO @ Tue, 27 Jun 2017 19:12:10: 30000000 INFO @ Tue, 27 Jun 2017 19:12:13: 30000000 INFO @ Tue, 27 Jun 2017 19:12:15: 32000000 INFO @ Tue, 27 Jun 2017 19:12:19: 31000000 INFO @ Tue, 27 Jun 2017 19:12:22: 31000000 INFO @ Tue, 27 Jun 2017 19:12:24: 33000000 INFO @ Tue, 27 Jun 2017 19:12:27: 32000000 INFO @ Tue, 27 Jun 2017 19:12:31: 32000000 INFO @ Tue, 27 Jun 2017 19:12:33: 34000000 INFO @ Tue, 27 Jun 2017 19:12:36: 33000000 INFO @ Tue, 27 Jun 2017 19:12:40: 33000000 INFO @ Tue, 27 Jun 2017 19:12:42: 35000000 INFO @ Tue, 27 Jun 2017 19:12:45: 34000000 INFO @ Tue, 27 Jun 2017 19:12:49: 34000000 INFO @ Tue, 27 Jun 2017 19:12:51: 36000000 INFO @ Tue, 27 Jun 2017 19:12:54: 35000000 INFO @ Tue, 27 Jun 2017 19:12:58: 35000000 INFO @ Tue, 27 Jun 2017 19:13:00: 37000000 INFO @ Tue, 27 Jun 2017 19:13:03: 36000000 INFO @ Tue, 27 Jun 2017 19:13:07: 36000000 INFO @ Tue, 27 Jun 2017 19:13:09: 38000000 INFO @ Tue, 27 Jun 2017 19:13:11: 37000000 INFO @ Tue, 27 Jun 2017 19:13:16: 37000000 INFO @ Tue, 27 Jun 2017 19:13:18: 39000000 INFO @ Tue, 27 Jun 2017 19:13:20: 38000000 INFO @ Tue, 27 Jun 2017 19:13:25: 38000000 INFO @ Tue, 27 Jun 2017 19:13:27: 40000000 INFO @ Tue, 27 Jun 2017 19:13:29: 39000000 INFO @ Tue, 27 Jun 2017 19:13:35: 39000000 INFO @ Tue, 27 Jun 2017 19:13:36: 41000000 INFO @ Tue, 27 Jun 2017 19:13:38: 40000000 INFO @ Tue, 27 Jun 2017 19:13:44: 40000000 INFO @ Tue, 27 Jun 2017 19:13:45: 42000000 INFO @ Tue, 27 Jun 2017 19:13:46: 41000000 INFO @ Tue, 27 Jun 2017 19:13:53: 41000000 INFO @ Tue, 27 Jun 2017 19:13:54: 43000000 INFO @ Tue, 27 Jun 2017 19:13:55: 42000000 INFO @ Tue, 27 Jun 2017 19:14:02: 42000000 INFO @ Tue, 27 Jun 2017 19:14:03: 44000000 INFO @ Tue, 27 Jun 2017 19:14:04: 43000000 INFO @ Tue, 27 Jun 2017 19:14:11: 43000000 INFO @ Tue, 27 Jun 2017 19:14:12: 45000000 INFO @ Tue, 27 Jun 2017 19:14:13: 44000000 INFO @ Tue, 27 Jun 2017 19:14:20: 44000000 INFO @ Tue, 27 Jun 2017 19:14:21: 46000000 INFO @ Tue, 27 Jun 2017 19:14:22: 45000000 INFO @ Tue, 27 Jun 2017 19:14:29: 45000000 INFO @ Tue, 27 Jun 2017 19:14:31: 46000000 INFO @ Tue, 27 Jun 2017 19:14:31: 47000000 INFO @ Tue, 27 Jun 2017 19:14:38: 46000000 INFO @ Tue, 27 Jun 2017 19:14:39: 47000000 INFO @ Tue, 27 Jun 2017 19:14:40: 48000000 INFO @ Tue, 27 Jun 2017 19:14:42: #1 tag size is determined as 61 bps INFO @ Tue, 27 Jun 2017 19:14:42: #1 tag size = 61 INFO @ Tue, 27 Jun 2017 19:14:42: #1 total tags in treatment: 48209251 INFO @ Tue, 27 Jun 2017 19:14:42: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 19:14:42: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 19:14:43: #1 tags after filtering in treatment: 48209251 INFO @ Tue, 27 Jun 2017 19:14:43: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 19:14:43: #1 finished! INFO @ Tue, 27 Jun 2017 19:14:43: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 19:14:43: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 19:14:46: #2 number of paired peaks: 0 WARNING @ Tue, 27 Jun 2017 19:14:46: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 19:14:46: Process for pairing-model is terminated! cat: SRX1342245.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1342245.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1342245.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1342245.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Tue, 27 Jun 2017 19:14:47: 47000000 INFO @ Tue, 27 Jun 2017 19:14:48: 48000000 INFO @ Tue, 27 Jun 2017 19:14:51: #1 tag size is determined as 61 bps INFO @ Tue, 27 Jun 2017 19:14:51: #1 tag size = 61 INFO @ Tue, 27 Jun 2017 19:14:51: #1 total tags in treatment: 48209251 INFO @ Tue, 27 Jun 2017 19:14:51: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 19:14:51: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 19:14:52: #1 tags after filtering in treatment: 48209251 INFO @ Tue, 27 Jun 2017 19:14:52: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 19:14:52: #1 finished! INFO @ Tue, 27 Jun 2017 19:14:52: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 19:14:52: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 19:14:55: #2 number of paired peaks: 0 WARNING @ Tue, 27 Jun 2017 19:14:55: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 19:14:55: Process for pairing-model is terminated! INFO @ Tue, 27 Jun 2017 19:14:55: 48000000 cat: SRX1342245.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1342245.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1342245.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1342245.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Tue, 27 Jun 2017 19:14:57: #1 tag size is determined as 61 bps INFO @ Tue, 27 Jun 2017 19:14:57: #1 tag size = 61 INFO @ Tue, 27 Jun 2017 19:14:57: #1 total tags in treatment: 48209251 INFO @ Tue, 27 Jun 2017 19:14:57: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 19:14:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 19:14:58: #1 tags after filtering in treatment: 48209251 INFO @ Tue, 27 Jun 2017 19:14:58: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 19:14:58: #1 finished! INFO @ Tue, 27 Jun 2017 19:14:58: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 19:14:58: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 19:15:01: #2 number of paired peaks: 0 WARNING @ Tue, 27 Jun 2017 19:15:01: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 19:15:01: Process for pairing-model is terminated! cat: SRX1342245.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1342245.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1342245.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1342245.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。