Job ID = 16440015 SRX = SRX13386239 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 42850760 spots for SRR17206322/SRR17206322.sra Written 42850760 spots for SRR17206322/SRR17206322.sra fastq に変換しました。 bowtie でマッピング中... Your job 16441006 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:01 Time loading mirror index: 00:00:00 Multiseed full-index search: 01:42:08 42850760 reads; of these: 42850760 (100.00%) were paired; of these: 6651708 (15.52%) aligned concordantly 0 times 27256661 (63.61%) aligned concordantly exactly 1 time 8942391 (20.87%) aligned concordantly >1 times ---- 6651708 pairs aligned concordantly 0 times; of these: 37901 (0.57%) aligned discordantly 1 time ---- 6613807 pairs aligned 0 times concordantly or discordantly; of these: 13227614 mates make up the pairs; of these: 12143737 (91.81%) aligned 0 times 771299 (5.83%) aligned exactly 1 time 312578 (2.36%) aligned >1 times 85.83% overall alignment rate Time searching: 01:42:09 Overall time: 01:42:09 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 32 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 10901166 / 36220351 = 0.3010 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 18:48:22: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX13386239/SRX13386239.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX13386239/SRX13386239.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX13386239/SRX13386239.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX13386239/SRX13386239.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 18:48:22: #1 read tag files... INFO @ Tue, 02 Aug 2022 18:48:22: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 18:48:31: 1000000 INFO @ Tue, 02 Aug 2022 18:48:40: 2000000 INFO @ Tue, 02 Aug 2022 18:48:48: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 18:48:52: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX13386239/SRX13386239.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX13386239/SRX13386239.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX13386239/SRX13386239.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX13386239/SRX13386239.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 18:48:52: #1 read tag files... INFO @ Tue, 02 Aug 2022 18:48:52: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 18:48:57: 4000000 INFO @ Tue, 02 Aug 2022 18:49:02: 1000000 INFO @ Tue, 02 Aug 2022 18:49:05: 5000000 INFO @ Tue, 02 Aug 2022 18:49:11: 2000000 INFO @ Tue, 02 Aug 2022 18:49:13: 6000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 18:49:21: 3000000 INFO @ Tue, 02 Aug 2022 18:49:22: 7000000 INFO @ Tue, 02 Aug 2022 18:49:22: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX13386239/SRX13386239.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX13386239/SRX13386239.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX13386239/SRX13386239.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX13386239/SRX13386239.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 18:49:22: #1 read tag files... INFO @ Tue, 02 Aug 2022 18:49:22: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 18:49:31: 8000000 INFO @ Tue, 02 Aug 2022 18:49:31: 4000000 INFO @ Tue, 02 Aug 2022 18:49:32: 1000000 INFO @ Tue, 02 Aug 2022 18:49:39: 9000000 INFO @ Tue, 02 Aug 2022 18:49:41: 5000000 INFO @ Tue, 02 Aug 2022 18:49:41: 2000000 INFO @ Tue, 02 Aug 2022 18:49:48: 10000000 INFO @ Tue, 02 Aug 2022 18:49:50: 3000000 INFO @ Tue, 02 Aug 2022 18:49:50: 6000000 INFO @ Tue, 02 Aug 2022 18:49:56: 11000000 INFO @ Tue, 02 Aug 2022 18:50:00: 4000000 INFO @ Tue, 02 Aug 2022 18:50:00: 7000000 INFO @ Tue, 02 Aug 2022 18:50:04: 12000000 INFO @ Tue, 02 Aug 2022 18:50:09: 5000000 INFO @ Tue, 02 Aug 2022 18:50:09: 8000000 INFO @ Tue, 02 Aug 2022 18:50:12: 13000000 INFO @ Tue, 02 Aug 2022 18:50:18: 6000000 INFO @ Tue, 02 Aug 2022 18:50:19: 9000000 INFO @ Tue, 02 Aug 2022 18:50:20: 14000000 INFO @ Tue, 02 Aug 2022 18:50:27: 7000000 INFO @ Tue, 02 Aug 2022 18:50:28: 15000000 INFO @ Tue, 02 Aug 2022 18:50:29: 10000000 INFO @ Tue, 02 Aug 2022 18:50:35: 16000000 INFO @ Tue, 02 Aug 2022 18:50:36: 8000000 INFO @ Tue, 02 Aug 2022 18:50:38: 11000000 INFO @ Tue, 02 Aug 2022 18:50:44: 17000000 INFO @ Tue, 02 Aug 2022 18:50:46: 9000000 INFO @ Tue, 02 Aug 2022 18:50:47: 12000000 INFO @ Tue, 02 Aug 2022 18:50:52: 18000000 INFO @ Tue, 02 Aug 2022 18:50:55: 10000000 INFO @ Tue, 02 Aug 2022 18:50:55: 13000000 INFO @ Tue, 02 Aug 2022 18:51:01: 19000000 INFO @ Tue, 02 Aug 2022 18:51:04: 14000000 INFO @ Tue, 02 Aug 2022 18:51:04: 11000000 INFO @ Tue, 02 Aug 2022 18:51:08: 20000000 INFO @ Tue, 02 Aug 2022 18:51:13: 15000000 INFO @ Tue, 02 Aug 2022 18:51:13: 12000000 INFO @ Tue, 02 Aug 2022 18:51:16: 21000000 INFO @ Tue, 02 Aug 2022 18:51:21: 13000000 INFO @ Tue, 02 Aug 2022 18:51:22: 16000000 INFO @ Tue, 02 Aug 2022 18:51:23: 22000000 INFO @ Tue, 02 Aug 2022 18:51:30: 14000000 INFO @ Tue, 02 Aug 2022 18:51:30: 23000000 INFO @ Tue, 02 Aug 2022 18:51:31: 17000000 INFO @ Tue, 02 Aug 2022 18:51:38: 24000000 INFO @ Tue, 02 Aug 2022 18:51:39: 15000000 INFO @ Tue, 02 Aug 2022 18:51:39: 18000000 INFO @ Tue, 02 Aug 2022 18:51:45: 25000000 INFO @ Tue, 02 Aug 2022 18:51:48: 16000000 INFO @ Tue, 02 Aug 2022 18:51:49: 19000000 INFO @ Tue, 02 Aug 2022 18:51:52: 26000000 INFO @ Tue, 02 Aug 2022 18:51:56: 17000000 INFO @ Tue, 02 Aug 2022 18:51:58: 20000000 INFO @ Tue, 02 Aug 2022 18:52:00: 27000000 INFO @ Tue, 02 Aug 2022 18:52:05: 18000000 INFO @ Tue, 02 Aug 2022 18:52:07: 21000000 INFO @ Tue, 02 Aug 2022 18:52:08: 28000000 INFO @ Tue, 02 Aug 2022 18:52:13: 19000000 INFO @ Tue, 02 Aug 2022 18:52:15: 22000000 INFO @ Tue, 02 Aug 2022 18:52:16: 29000000 INFO @ Tue, 02 Aug 2022 18:52:22: 20000000 INFO @ Tue, 02 Aug 2022 18:52:23: 23000000 INFO @ Tue, 02 Aug 2022 18:52:24: 30000000 INFO @ Tue, 02 Aug 2022 18:52:31: 21000000 INFO @ Tue, 02 Aug 2022 18:52:31: 24000000 INFO @ Tue, 02 Aug 2022 18:52:32: 31000000 INFO @ Tue, 02 Aug 2022 18:52:39: 32000000 INFO @ Tue, 02 Aug 2022 18:52:40: 22000000 INFO @ Tue, 02 Aug 2022 18:52:40: 25000000 INFO @ Tue, 02 Aug 2022 18:52:47: 33000000 INFO @ Tue, 02 Aug 2022 18:52:48: 23000000 INFO @ Tue, 02 Aug 2022 18:52:49: 26000000 INFO @ Tue, 02 Aug 2022 18:52:56: 34000000 INFO @ Tue, 02 Aug 2022 18:52:57: 24000000 INFO @ Tue, 02 Aug 2022 18:52:57: 27000000 INFO @ Tue, 02 Aug 2022 18:53:04: 35000000 INFO @ Tue, 02 Aug 2022 18:53:05: 25000000 INFO @ Tue, 02 Aug 2022 18:53:06: 28000000 INFO @ Tue, 02 Aug 2022 18:53:12: 36000000 INFO @ Tue, 02 Aug 2022 18:53:14: 26000000 INFO @ Tue, 02 Aug 2022 18:53:15: 29000000 INFO @ Tue, 02 Aug 2022 18:53:20: 37000000 INFO @ Tue, 02 Aug 2022 18:53:23: 30000000 INFO @ Tue, 02 Aug 2022 18:53:23: 27000000 INFO @ Tue, 02 Aug 2022 18:53:28: 38000000 INFO @ Tue, 02 Aug 2022 18:53:31: 31000000 INFO @ Tue, 02 Aug 2022 18:53:32: 28000000 INFO @ Tue, 02 Aug 2022 18:53:36: 39000000 INFO @ Tue, 02 Aug 2022 18:53:40: 32000000 INFO @ Tue, 02 Aug 2022 18:53:41: 29000000 INFO @ Tue, 02 Aug 2022 18:53:43: 40000000 INFO @ Tue, 02 Aug 2022 18:53:48: 33000000 INFO @ Tue, 02 Aug 2022 18:53:50: 30000000 INFO @ Tue, 02 Aug 2022 18:53:51: 41000000 INFO @ Tue, 02 Aug 2022 18:53:56: 34000000 INFO @ Tue, 02 Aug 2022 18:53:58: 42000000 INFO @ Tue, 02 Aug 2022 18:53:59: 31000000 INFO @ Tue, 02 Aug 2022 18:54:04: 35000000 INFO @ Tue, 02 Aug 2022 18:54:06: 43000000 INFO @ Tue, 02 Aug 2022 18:54:07: 32000000 INFO @ Tue, 02 Aug 2022 18:54:12: 36000000 INFO @ Tue, 02 Aug 2022 18:54:14: 44000000 INFO @ Tue, 02 Aug 2022 18:54:15: 33000000 INFO @ Tue, 02 Aug 2022 18:54:20: 37000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 02 Aug 2022 18:54:21: 45000000 INFO @ Tue, 02 Aug 2022 18:54:23: 34000000 INFO @ Tue, 02 Aug 2022 18:54:28: 38000000 INFO @ Tue, 02 Aug 2022 18:54:29: 46000000 INFO @ Tue, 02 Aug 2022 18:54:31: 35000000 INFO @ Tue, 02 Aug 2022 18:54:36: 39000000 INFO @ Tue, 02 Aug 2022 18:54:37: 47000000 INFO @ Tue, 02 Aug 2022 18:54:39: 36000000 INFO @ Tue, 02 Aug 2022 18:54:44: 40000000 INFO @ Tue, 02 Aug 2022 18:54:45: 48000000 INFO @ Tue, 02 Aug 2022 18:54:47: 37000000 INFO @ Tue, 02 Aug 2022 18:54:52: 41000000 INFO @ Tue, 02 Aug 2022 18:54:53: 49000000 INFO @ Tue, 02 Aug 2022 18:54:55: 38000000 INFO @ Tue, 02 Aug 2022 18:54:59: 42000000 INFO @ Tue, 02 Aug 2022 18:55:00: 50000000 INFO @ Tue, 02 Aug 2022 18:55:03: 39000000 INFO @ Tue, 02 Aug 2022 18:55:07: 43000000 INFO @ Tue, 02 Aug 2022 18:55:07: 51000000 INFO @ Tue, 02 Aug 2022 18:55:11: 40000000 INFO @ Tue, 02 Aug 2022 18:55:12: #1 tag size is determined as 51 bps INFO @ Tue, 02 Aug 2022 18:55:12: #1 tag size = 51 INFO @ Tue, 02 Aug 2022 18:55:12: #1 total tags in treatment: 25305603 INFO @ Tue, 02 Aug 2022 18:55:12: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 18:55:12: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 18:55:13: #1 tags after filtering in treatment: 19371447 INFO @ Tue, 02 Aug 2022 18:55:13: #1 Redundant rate of treatment: 0.23 INFO @ Tue, 02 Aug 2022 18:55:13: #1 finished! INFO @ Tue, 02 Aug 2022 18:55:13: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 18:55:13: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 18:55:14: 44000000 INFO @ Tue, 02 Aug 2022 18:55:15: #2 number of paired peaks: 34 WARNING @ Tue, 02 Aug 2022 18:55:15: Too few paired peaks (34) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 18:55:15: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX13386239/SRX13386239.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13386239/SRX13386239.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13386239/SRX13386239.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13386239/SRX13386239.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 18:55:19: 41000000 INFO @ Tue, 02 Aug 2022 18:55:21: 45000000 INFO @ Tue, 02 Aug 2022 18:55:26: 42000000 INFO @ Tue, 02 Aug 2022 18:55:29: 46000000 INFO @ Tue, 02 Aug 2022 18:55:34: 43000000 INFO @ Tue, 02 Aug 2022 18:55:37: 47000000 INFO @ Tue, 02 Aug 2022 18:55:42: 44000000 INFO @ Tue, 02 Aug 2022 18:55:45: 48000000 INFO @ Tue, 02 Aug 2022 18:55:50: 45000000 INFO @ Tue, 02 Aug 2022 18:55:52: 49000000 INFO @ Tue, 02 Aug 2022 18:55:58: 46000000 INFO @ Tue, 02 Aug 2022 18:56:00: 50000000 INFO @ Tue, 02 Aug 2022 18:56:06: 47000000 INFO @ Tue, 02 Aug 2022 18:56:08: 51000000 BigWig に変換しました。 INFO @ Tue, 02 Aug 2022 18:56:14: #1 tag size is determined as 51 bps INFO @ Tue, 02 Aug 2022 18:56:14: #1 tag size = 51 INFO @ Tue, 02 Aug 2022 18:56:14: #1 total tags in treatment: 25305603 INFO @ Tue, 02 Aug 2022 18:56:14: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 18:56:14: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 18:56:14: #1 tags after filtering in treatment: 19371447 INFO @ Tue, 02 Aug 2022 18:56:14: #1 Redundant rate of treatment: 0.23 INFO @ Tue, 02 Aug 2022 18:56:14: #1 finished! INFO @ Tue, 02 Aug 2022 18:56:14: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 18:56:14: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 18:56:15: 48000000 INFO @ Tue, 02 Aug 2022 18:56:16: #2 number of paired peaks: 34 WARNING @ Tue, 02 Aug 2022 18:56:16: Too few paired peaks (34) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 18:56:16: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX13386239/SRX13386239.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13386239/SRX13386239.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13386239/SRX13386239.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13386239/SRX13386239.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 18:56:23: 49000000 INFO @ Tue, 02 Aug 2022 18:56:31: 50000000 INFO @ Tue, 02 Aug 2022 18:56:39: 51000000 INFO @ Tue, 02 Aug 2022 18:56:46: #1 tag size is determined as 51 bps INFO @ Tue, 02 Aug 2022 18:56:46: #1 tag size = 51 INFO @ Tue, 02 Aug 2022 18:56:46: #1 total tags in treatment: 25305603 INFO @ Tue, 02 Aug 2022 18:56:46: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 18:56:46: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 18:56:46: #1 tags after filtering in treatment: 19371447 INFO @ Tue, 02 Aug 2022 18:56:46: #1 Redundant rate of treatment: 0.23 INFO @ Tue, 02 Aug 2022 18:56:46: #1 finished! INFO @ Tue, 02 Aug 2022 18:56:46: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 18:56:46: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 18:56:48: #2 number of paired peaks: 34 WARNING @ Tue, 02 Aug 2022 18:56:48: Too few paired peaks (34) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 18:56:48: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX13386239/SRX13386239.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13386239/SRX13386239.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13386239/SRX13386239.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13386239/SRX13386239.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling