Job ID = 16439341 SRX = SRX13227899 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 35987896 spots for SRR17038012/SRR17038012.sra Written 35987896 spots for SRR17038012/SRR17038012.sra fastq に変換しました。 bowtie でマッピング中... Your job 16439489 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:01 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:17:57 35987896 reads; of these: 35987896 (100.00%) were unpaired; of these: 6459626 (17.95%) aligned 0 times 22472771 (62.45%) aligned exactly 1 time 7055499 (19.61%) aligned >1 times 82.05% overall alignment rate Time searching: 00:17:59 Overall time: 00:17:59 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 3787546 / 29528270 = 0.1283 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 15:28:03: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX13227899/SRX13227899.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX13227899/SRX13227899.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX13227899/SRX13227899.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX13227899/SRX13227899.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 15:28:03: #1 read tag files... INFO @ Tue, 02 Aug 2022 15:28:03: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 15:28:14: 1000000 INFO @ Tue, 02 Aug 2022 15:28:25: 2000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 15:28:33: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX13227899/SRX13227899.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX13227899/SRX13227899.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX13227899/SRX13227899.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX13227899/SRX13227899.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 15:28:33: #1 read tag files... INFO @ Tue, 02 Aug 2022 15:28:33: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 15:28:35: 3000000 INFO @ Tue, 02 Aug 2022 15:28:45: 1000000 INFO @ Tue, 02 Aug 2022 15:28:46: 4000000 INFO @ Tue, 02 Aug 2022 15:28:56: 2000000 INFO @ Tue, 02 Aug 2022 15:28:57: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 15:29:03: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX13227899/SRX13227899.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX13227899/SRX13227899.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX13227899/SRX13227899.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX13227899/SRX13227899.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 15:29:03: #1 read tag files... INFO @ Tue, 02 Aug 2022 15:29:03: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 15:29:08: 3000000 INFO @ Tue, 02 Aug 2022 15:29:08: 6000000 INFO @ Tue, 02 Aug 2022 15:29:16: 1000000 INFO @ Tue, 02 Aug 2022 15:29:19: 7000000 INFO @ Tue, 02 Aug 2022 15:29:20: 4000000 INFO @ Tue, 02 Aug 2022 15:29:28: 2000000 INFO @ Tue, 02 Aug 2022 15:29:30: 8000000 INFO @ Tue, 02 Aug 2022 15:29:31: 5000000 INFO @ Tue, 02 Aug 2022 15:29:40: 3000000 INFO @ Tue, 02 Aug 2022 15:29:42: 9000000 INFO @ Tue, 02 Aug 2022 15:29:43: 6000000 INFO @ Tue, 02 Aug 2022 15:29:53: 4000000 INFO @ Tue, 02 Aug 2022 15:29:53: 10000000 INFO @ Tue, 02 Aug 2022 15:29:54: 7000000 INFO @ Tue, 02 Aug 2022 15:30:05: 11000000 INFO @ Tue, 02 Aug 2022 15:30:05: 5000000 INFO @ Tue, 02 Aug 2022 15:30:06: 8000000 INFO @ Tue, 02 Aug 2022 15:30:16: 12000000 INFO @ Tue, 02 Aug 2022 15:30:17: 6000000 INFO @ Tue, 02 Aug 2022 15:30:18: 9000000 INFO @ Tue, 02 Aug 2022 15:30:27: 13000000 INFO @ Tue, 02 Aug 2022 15:30:29: 7000000 INFO @ Tue, 02 Aug 2022 15:30:30: 10000000 INFO @ Tue, 02 Aug 2022 15:30:39: 14000000 INFO @ Tue, 02 Aug 2022 15:30:41: 8000000 INFO @ Tue, 02 Aug 2022 15:30:42: 11000000 INFO @ Tue, 02 Aug 2022 15:30:50: 15000000 INFO @ Tue, 02 Aug 2022 15:30:53: 12000000 INFO @ Tue, 02 Aug 2022 15:30:54: 9000000 INFO @ Tue, 02 Aug 2022 15:31:01: 16000000 INFO @ Tue, 02 Aug 2022 15:31:04: 13000000 INFO @ Tue, 02 Aug 2022 15:31:07: 10000000 INFO @ Tue, 02 Aug 2022 15:31:13: 17000000 INFO @ Tue, 02 Aug 2022 15:31:16: 14000000 INFO @ Tue, 02 Aug 2022 15:31:20: 11000000 INFO @ Tue, 02 Aug 2022 15:31:24: 18000000 INFO @ Tue, 02 Aug 2022 15:31:28: 15000000 INFO @ Tue, 02 Aug 2022 15:31:33: 12000000 INFO @ Tue, 02 Aug 2022 15:31:36: 19000000 INFO @ Tue, 02 Aug 2022 15:31:41: 16000000 INFO @ Tue, 02 Aug 2022 15:31:44: 13000000 INFO @ Tue, 02 Aug 2022 15:31:47: 20000000 INFO @ Tue, 02 Aug 2022 15:31:53: 17000000 INFO @ Tue, 02 Aug 2022 15:31:55: 14000000 INFO @ Tue, 02 Aug 2022 15:31:58: 21000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 02 Aug 2022 15:32:06: 18000000 INFO @ Tue, 02 Aug 2022 15:32:07: 15000000 INFO @ Tue, 02 Aug 2022 15:32:10: 22000000 INFO @ Tue, 02 Aug 2022 15:32:18: 19000000 INFO @ Tue, 02 Aug 2022 15:32:19: 16000000 INFO @ Tue, 02 Aug 2022 15:32:22: 23000000 INFO @ Tue, 02 Aug 2022 15:32:30: 20000000 INFO @ Tue, 02 Aug 2022 15:32:31: 17000000 INFO @ Tue, 02 Aug 2022 15:32:34: 24000000 INFO @ Tue, 02 Aug 2022 15:32:42: 18000000 INFO @ Tue, 02 Aug 2022 15:32:44: 21000000 INFO @ Tue, 02 Aug 2022 15:32:46: 25000000 INFO @ Tue, 02 Aug 2022 15:32:53: #1 tag size is determined as 74 bps INFO @ Tue, 02 Aug 2022 15:32:53: #1 tag size = 74 INFO @ Tue, 02 Aug 2022 15:32:53: #1 total tags in treatment: 25740724 INFO @ Tue, 02 Aug 2022 15:32:53: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 15:32:53: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 15:32:54: 19000000 INFO @ Tue, 02 Aug 2022 15:32:54: #1 tags after filtering in treatment: 25740724 INFO @ Tue, 02 Aug 2022 15:32:54: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 15:32:54: #1 finished! INFO @ Tue, 02 Aug 2022 15:32:54: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 15:32:54: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 15:32:56: #2 number of paired peaks: 6 WARNING @ Tue, 02 Aug 2022 15:32:56: Too few paired peaks (6) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 15:32:56: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX13227899/SRX13227899.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13227899/SRX13227899.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13227899/SRX13227899.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13227899/SRX13227899.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 15:32:56: 22000000 INFO @ Tue, 02 Aug 2022 15:33:05: 20000000 INFO @ Tue, 02 Aug 2022 15:33:09: 23000000 INFO @ Tue, 02 Aug 2022 15:33:17: 21000000 INFO @ Tue, 02 Aug 2022 15:33:21: 24000000 INFO @ Tue, 02 Aug 2022 15:33:29: 22000000 BigWig に変換しました。 INFO @ Tue, 02 Aug 2022 15:33:33: 25000000 INFO @ Tue, 02 Aug 2022 15:33:39: 23000000 INFO @ Tue, 02 Aug 2022 15:33:42: #1 tag size is determined as 74 bps INFO @ Tue, 02 Aug 2022 15:33:42: #1 tag size = 74 INFO @ Tue, 02 Aug 2022 15:33:42: #1 total tags in treatment: 25740724 INFO @ Tue, 02 Aug 2022 15:33:42: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 15:33:42: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 15:33:43: #1 tags after filtering in treatment: 25740724 INFO @ Tue, 02 Aug 2022 15:33:43: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 15:33:43: #1 finished! INFO @ Tue, 02 Aug 2022 15:33:43: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 15:33:43: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 15:33:45: #2 number of paired peaks: 6 WARNING @ Tue, 02 Aug 2022 15:33:45: Too few paired peaks (6) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 15:33:45: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX13227899/SRX13227899.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13227899/SRX13227899.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13227899/SRX13227899.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13227899/SRX13227899.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 15:33:50: 24000000 INFO @ Tue, 02 Aug 2022 15:34:02: 25000000 INFO @ Tue, 02 Aug 2022 15:34:11: #1 tag size is determined as 74 bps INFO @ Tue, 02 Aug 2022 15:34:11: #1 tag size = 74 INFO @ Tue, 02 Aug 2022 15:34:11: #1 total tags in treatment: 25740724 INFO @ Tue, 02 Aug 2022 15:34:11: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 15:34:11: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 15:34:11: #1 tags after filtering in treatment: 25740724 INFO @ Tue, 02 Aug 2022 15:34:11: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 15:34:11: #1 finished! INFO @ Tue, 02 Aug 2022 15:34:11: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 15:34:11: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 15:34:13: #2 number of paired peaks: 6 WARNING @ Tue, 02 Aug 2022 15:34:13: Too few paired peaks (6) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 15:34:13: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX13227899/SRX13227899.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13227899/SRX13227899.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13227899/SRX13227899.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13227899/SRX13227899.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling