Job ID = 16439340 SRX = SRX13227898 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 39029700 spots for SRR17038011/SRR17038011.sra Written 39029700 spots for SRR17038011/SRR17038011.sra fastq に変換しました。 bowtie でマッピング中... Your job 16439492 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:18:55 39029700 reads; of these: 39029700 (100.00%) were unpaired; of these: 984193 (2.52%) aligned 0 times 27028895 (69.25%) aligned exactly 1 time 11016612 (28.23%) aligned >1 times 97.48% overall alignment rate Time searching: 00:18:55 Overall time: 00:18:55 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 5045019 / 38045507 = 0.1326 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 15:30:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX13227898/SRX13227898.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX13227898/SRX13227898.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX13227898/SRX13227898.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX13227898/SRX13227898.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 15:30:30: #1 read tag files... INFO @ Tue, 02 Aug 2022 15:30:30: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 15:30:38: 1000000 INFO @ Tue, 02 Aug 2022 15:30:46: 2000000 INFO @ Tue, 02 Aug 2022 15:30:54: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 15:30:58: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX13227898/SRX13227898.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX13227898/SRX13227898.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX13227898/SRX13227898.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX13227898/SRX13227898.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 15:30:58: #1 read tag files... INFO @ Tue, 02 Aug 2022 15:30:58: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 15:31:02: 4000000 INFO @ Tue, 02 Aug 2022 15:31:05: 1000000 INFO @ Tue, 02 Aug 2022 15:31:10: 5000000 INFO @ Tue, 02 Aug 2022 15:31:12: 2000000 INFO @ Tue, 02 Aug 2022 15:31:18: 6000000 INFO @ Tue, 02 Aug 2022 15:31:18: 3000000 INFO @ Tue, 02 Aug 2022 15:31:25: 4000000 INFO @ Tue, 02 Aug 2022 15:31:26: 7000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 15:31:29: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX13227898/SRX13227898.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX13227898/SRX13227898.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX13227898/SRX13227898.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX13227898/SRX13227898.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 15:31:29: #1 read tag files... INFO @ Tue, 02 Aug 2022 15:31:29: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 15:31:32: 5000000 INFO @ Tue, 02 Aug 2022 15:31:35: 8000000 INFO @ Tue, 02 Aug 2022 15:31:36: 1000000 INFO @ Tue, 02 Aug 2022 15:31:39: 6000000 INFO @ Tue, 02 Aug 2022 15:31:43: 9000000 INFO @ Tue, 02 Aug 2022 15:31:43: 2000000 INFO @ Tue, 02 Aug 2022 15:31:47: 7000000 INFO @ Tue, 02 Aug 2022 15:31:51: 3000000 INFO @ Tue, 02 Aug 2022 15:31:51: 10000000 INFO @ Tue, 02 Aug 2022 15:31:55: 8000000 INFO @ Tue, 02 Aug 2022 15:31:59: 4000000 INFO @ Tue, 02 Aug 2022 15:32:01: 11000000 INFO @ Tue, 02 Aug 2022 15:32:02: 9000000 INFO @ Tue, 02 Aug 2022 15:32:06: 5000000 INFO @ Tue, 02 Aug 2022 15:32:09: 10000000 INFO @ Tue, 02 Aug 2022 15:32:10: 12000000 INFO @ Tue, 02 Aug 2022 15:32:13: 6000000 INFO @ Tue, 02 Aug 2022 15:32:17: 11000000 INFO @ Tue, 02 Aug 2022 15:32:18: 13000000 INFO @ Tue, 02 Aug 2022 15:32:21: 7000000 INFO @ Tue, 02 Aug 2022 15:32:24: 12000000 INFO @ Tue, 02 Aug 2022 15:32:27: 14000000 INFO @ Tue, 02 Aug 2022 15:32:28: 8000000 INFO @ Tue, 02 Aug 2022 15:32:32: 13000000 INFO @ Tue, 02 Aug 2022 15:32:35: 9000000 INFO @ Tue, 02 Aug 2022 15:32:36: 15000000 INFO @ Tue, 02 Aug 2022 15:32:39: 14000000 INFO @ Tue, 02 Aug 2022 15:32:42: 10000000 INFO @ Tue, 02 Aug 2022 15:32:44: 16000000 INFO @ Tue, 02 Aug 2022 15:32:46: 15000000 INFO @ Tue, 02 Aug 2022 15:32:49: 11000000 INFO @ Tue, 02 Aug 2022 15:32:53: 17000000 INFO @ Tue, 02 Aug 2022 15:32:54: 16000000 INFO @ Tue, 02 Aug 2022 15:32:57: 12000000 INFO @ Tue, 02 Aug 2022 15:33:01: 17000000 INFO @ Tue, 02 Aug 2022 15:33:02: 18000000 INFO @ Tue, 02 Aug 2022 15:33:04: 13000000 INFO @ Tue, 02 Aug 2022 15:33:09: 18000000 INFO @ Tue, 02 Aug 2022 15:33:11: 19000000 INFO @ Tue, 02 Aug 2022 15:33:11: 14000000 INFO @ Tue, 02 Aug 2022 15:33:16: 19000000 INFO @ Tue, 02 Aug 2022 15:33:19: 15000000 INFO @ Tue, 02 Aug 2022 15:33:20: 20000000 INFO @ Tue, 02 Aug 2022 15:33:23: 20000000 INFO @ Tue, 02 Aug 2022 15:33:26: 16000000 INFO @ Tue, 02 Aug 2022 15:33:28: 21000000 INFO @ Tue, 02 Aug 2022 15:33:31: 21000000 INFO @ Tue, 02 Aug 2022 15:33:34: 17000000 INFO @ Tue, 02 Aug 2022 15:33:37: 22000000 INFO @ Tue, 02 Aug 2022 15:33:38: 22000000 INFO @ Tue, 02 Aug 2022 15:33:41: 18000000 INFO @ Tue, 02 Aug 2022 15:33:46: 23000000 INFO @ Tue, 02 Aug 2022 15:33:46: 23000000 INFO @ Tue, 02 Aug 2022 15:33:49: 19000000 INFO @ Tue, 02 Aug 2022 15:33:53: 24000000 INFO @ Tue, 02 Aug 2022 15:33:55: 24000000 INFO @ Tue, 02 Aug 2022 15:33:55: 20000000 INFO @ Tue, 02 Aug 2022 15:34:01: 25000000 INFO @ Tue, 02 Aug 2022 15:34:03: 21000000 INFO @ Tue, 02 Aug 2022 15:34:04: 25000000 INFO @ Tue, 02 Aug 2022 15:34:10: 26000000 INFO @ Tue, 02 Aug 2022 15:34:11: 22000000 INFO @ Tue, 02 Aug 2022 15:34:13: 26000000 INFO @ Tue, 02 Aug 2022 15:34:19: 27000000 INFO @ Tue, 02 Aug 2022 15:34:19: 23000000 INFO @ Tue, 02 Aug 2022 15:34:22: 27000000 INFO @ Tue, 02 Aug 2022 15:34:27: 28000000 INFO @ Tue, 02 Aug 2022 15:34:28: 24000000 INFO @ Tue, 02 Aug 2022 15:34:31: 28000000 INFO @ Tue, 02 Aug 2022 15:34:36: 29000000 INFO @ Tue, 02 Aug 2022 15:34:37: 25000000 INFO @ Tue, 02 Aug 2022 15:34:40: 29000000 INFO @ Tue, 02 Aug 2022 15:34:45: 30000000 INFO @ Tue, 02 Aug 2022 15:34:45: 26000000 INFO @ Tue, 02 Aug 2022 15:34:49: 30000000 INFO @ Tue, 02 Aug 2022 15:34:53: 27000000 INFO @ Tue, 02 Aug 2022 15:34:53: 31000000 INFO @ Tue, 02 Aug 2022 15:34:59: 31000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 02 Aug 2022 15:35:01: 28000000 INFO @ Tue, 02 Aug 2022 15:35:02: 32000000 INFO @ Tue, 02 Aug 2022 15:35:09: 32000000 INFO @ Tue, 02 Aug 2022 15:35:10: 29000000 INFO @ Tue, 02 Aug 2022 15:35:11: 33000000 INFO @ Tue, 02 Aug 2022 15:35:11: #1 tag size is determined as 70 bps INFO @ Tue, 02 Aug 2022 15:35:11: #1 tag size = 70 INFO @ Tue, 02 Aug 2022 15:35:11: #1 total tags in treatment: 33000488 INFO @ Tue, 02 Aug 2022 15:35:11: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 15:35:11: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 15:35:12: #1 tags after filtering in treatment: 33000488 INFO @ Tue, 02 Aug 2022 15:35:12: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 15:35:12: #1 finished! INFO @ Tue, 02 Aug 2022 15:35:12: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 15:35:12: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 15:35:14: #2 number of paired peaks: 51 WARNING @ Tue, 02 Aug 2022 15:35:14: Too few paired peaks (51) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 15:35:14: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX13227898/SRX13227898.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 10 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13227898/SRX13227898.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13227898/SRX13227898.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13227898/SRX13227898.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 15:35:18: 33000000 INFO @ Tue, 02 Aug 2022 15:35:18: #1 tag size is determined as 70 bps INFO @ Tue, 02 Aug 2022 15:35:18: #1 tag size = 70 INFO @ Tue, 02 Aug 2022 15:35:18: #1 total tags in treatment: 33000488 INFO @ Tue, 02 Aug 2022 15:35:18: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 15:35:18: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 15:35:18: 30000000 INFO @ Tue, 02 Aug 2022 15:35:19: #1 tags after filtering in treatment: 33000488 INFO @ Tue, 02 Aug 2022 15:35:19: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 15:35:19: #1 finished! INFO @ Tue, 02 Aug 2022 15:35:19: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 15:35:19: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 15:35:21: #2 number of paired peaks: 51 WARNING @ Tue, 02 Aug 2022 15:35:21: Too few paired peaks (51) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 15:35:21: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX13227898/SRX13227898.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 23 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13227898/SRX13227898.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13227898/SRX13227898.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13227898/SRX13227898.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 15:35:27: 31000000 INFO @ Tue, 02 Aug 2022 15:35:35: 32000000 INFO @ Tue, 02 Aug 2022 15:35:43: 33000000 INFO @ Tue, 02 Aug 2022 15:35:43: #1 tag size is determined as 70 bps INFO @ Tue, 02 Aug 2022 15:35:43: #1 tag size = 70 INFO @ Tue, 02 Aug 2022 15:35:43: #1 total tags in treatment: 33000488 INFO @ Tue, 02 Aug 2022 15:35:43: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 15:35:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 15:35:44: #1 tags after filtering in treatment: 33000488 INFO @ Tue, 02 Aug 2022 15:35:44: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 15:35:44: #1 finished! INFO @ Tue, 02 Aug 2022 15:35:44: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 15:35:44: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 15:35:46: #2 number of paired peaks: 51 WARNING @ Tue, 02 Aug 2022 15:35:46: Too few paired peaks (51) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 15:35:46: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX13227898/SRX13227898.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13227898/SRX13227898.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13227898/SRX13227898.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13227898/SRX13227898.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。