Job ID = 16439339 SRX = SRX13227897 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 36608904 spots for SRR17038010/SRR17038010.sra Written 36608904 spots for SRR17038010/SRR17038010.sra fastq に変換しました。 bowtie でマッピング中... Your job 16439471 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:16:20 36608904 reads; of these: 36608904 (100.00%) were unpaired; of these: 1043790 (2.85%) aligned 0 times 26633418 (72.75%) aligned exactly 1 time 8931696 (24.40%) aligned >1 times 97.15% overall alignment rate Time searching: 00:16:21 Overall time: 00:16:21 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 4307706 / 35565114 = 0.1211 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 15:26:18: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX13227897/SRX13227897.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX13227897/SRX13227897.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX13227897/SRX13227897.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX13227897/SRX13227897.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 15:26:18: #1 read tag files... INFO @ Tue, 02 Aug 2022 15:26:18: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 15:26:27: 1000000 INFO @ Tue, 02 Aug 2022 15:26:37: 2000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 15:26:46: 3000000 INFO @ Tue, 02 Aug 2022 15:26:48: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX13227897/SRX13227897.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX13227897/SRX13227897.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX13227897/SRX13227897.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX13227897/SRX13227897.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 15:26:48: #1 read tag files... INFO @ Tue, 02 Aug 2022 15:26:48: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 15:26:54: 4000000 INFO @ Tue, 02 Aug 2022 15:26:56: 1000000 INFO @ Tue, 02 Aug 2022 15:27:03: 5000000 INFO @ Tue, 02 Aug 2022 15:27:05: 2000000 INFO @ Tue, 02 Aug 2022 15:27:12: 6000000 INFO @ Tue, 02 Aug 2022 15:27:14: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 15:27:18: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX13227897/SRX13227897.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX13227897/SRX13227897.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX13227897/SRX13227897.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX13227897/SRX13227897.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 15:27:18: #1 read tag files... INFO @ Tue, 02 Aug 2022 15:27:18: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 15:27:21: 7000000 INFO @ Tue, 02 Aug 2022 15:27:22: 4000000 INFO @ Tue, 02 Aug 2022 15:27:26: 1000000 INFO @ Tue, 02 Aug 2022 15:27:29: 8000000 INFO @ Tue, 02 Aug 2022 15:27:31: 5000000 INFO @ Tue, 02 Aug 2022 15:27:35: 2000000 INFO @ Tue, 02 Aug 2022 15:27:38: 9000000 INFO @ Tue, 02 Aug 2022 15:27:39: 6000000 INFO @ Tue, 02 Aug 2022 15:27:43: 3000000 INFO @ Tue, 02 Aug 2022 15:27:47: 10000000 INFO @ Tue, 02 Aug 2022 15:27:48: 7000000 INFO @ Tue, 02 Aug 2022 15:27:52: 4000000 INFO @ Tue, 02 Aug 2022 15:27:55: 11000000 INFO @ Tue, 02 Aug 2022 15:27:56: 8000000 INFO @ Tue, 02 Aug 2022 15:28:00: 5000000 INFO @ Tue, 02 Aug 2022 15:28:04: 12000000 INFO @ Tue, 02 Aug 2022 15:28:04: 9000000 INFO @ Tue, 02 Aug 2022 15:28:09: 6000000 INFO @ Tue, 02 Aug 2022 15:28:13: 10000000 INFO @ Tue, 02 Aug 2022 15:28:13: 13000000 INFO @ Tue, 02 Aug 2022 15:28:17: 7000000 INFO @ Tue, 02 Aug 2022 15:28:21: 11000000 INFO @ Tue, 02 Aug 2022 15:28:21: 14000000 INFO @ Tue, 02 Aug 2022 15:28:25: 8000000 INFO @ Tue, 02 Aug 2022 15:28:29: 12000000 INFO @ Tue, 02 Aug 2022 15:28:30: 15000000 INFO @ Tue, 02 Aug 2022 15:28:33: 9000000 INFO @ Tue, 02 Aug 2022 15:28:37: 13000000 INFO @ Tue, 02 Aug 2022 15:28:38: 16000000 INFO @ Tue, 02 Aug 2022 15:28:42: 10000000 INFO @ Tue, 02 Aug 2022 15:28:47: 14000000 INFO @ Tue, 02 Aug 2022 15:28:47: 17000000 INFO @ Tue, 02 Aug 2022 15:28:51: 11000000 INFO @ Tue, 02 Aug 2022 15:28:55: 18000000 INFO @ Tue, 02 Aug 2022 15:28:55: 15000000 INFO @ Tue, 02 Aug 2022 15:28:59: 12000000 INFO @ Tue, 02 Aug 2022 15:29:03: 16000000 INFO @ Tue, 02 Aug 2022 15:29:03: 19000000 INFO @ Tue, 02 Aug 2022 15:29:08: 13000000 INFO @ Tue, 02 Aug 2022 15:29:11: 20000000 INFO @ Tue, 02 Aug 2022 15:29:11: 17000000 INFO @ Tue, 02 Aug 2022 15:29:16: 14000000 INFO @ Tue, 02 Aug 2022 15:29:19: 18000000 INFO @ Tue, 02 Aug 2022 15:29:19: 21000000 INFO @ Tue, 02 Aug 2022 15:29:24: 15000000 INFO @ Tue, 02 Aug 2022 15:29:28: 19000000 INFO @ Tue, 02 Aug 2022 15:29:28: 22000000 INFO @ Tue, 02 Aug 2022 15:29:32: 16000000 INFO @ Tue, 02 Aug 2022 15:29:36: 20000000 INFO @ Tue, 02 Aug 2022 15:29:36: 23000000 INFO @ Tue, 02 Aug 2022 15:29:41: 17000000 INFO @ Tue, 02 Aug 2022 15:29:44: 21000000 INFO @ Tue, 02 Aug 2022 15:29:45: 24000000 INFO @ Tue, 02 Aug 2022 15:29:49: 18000000 INFO @ Tue, 02 Aug 2022 15:29:52: 22000000 INFO @ Tue, 02 Aug 2022 15:29:53: 25000000 INFO @ Tue, 02 Aug 2022 15:29:57: 19000000 INFO @ Tue, 02 Aug 2022 15:30:00: 23000000 INFO @ Tue, 02 Aug 2022 15:30:01: 26000000 INFO @ Tue, 02 Aug 2022 15:30:05: 20000000 INFO @ Tue, 02 Aug 2022 15:30:09: 24000000 INFO @ Tue, 02 Aug 2022 15:30:10: 27000000 INFO @ Tue, 02 Aug 2022 15:30:13: 21000000 INFO @ Tue, 02 Aug 2022 15:30:17: 25000000 INFO @ Tue, 02 Aug 2022 15:30:19: 28000000 INFO @ Tue, 02 Aug 2022 15:30:21: 22000000 INFO @ Tue, 02 Aug 2022 15:30:25: 26000000 INFO @ Tue, 02 Aug 2022 15:30:27: 29000000 INFO @ Tue, 02 Aug 2022 15:30:30: 23000000 INFO @ Tue, 02 Aug 2022 15:30:34: 27000000 INFO @ Tue, 02 Aug 2022 15:30:36: 30000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 02 Aug 2022 15:30:38: 24000000 INFO @ Tue, 02 Aug 2022 15:30:42: 28000000 INFO @ Tue, 02 Aug 2022 15:30:45: 31000000 INFO @ Tue, 02 Aug 2022 15:30:46: 25000000 INFO @ Tue, 02 Aug 2022 15:30:47: #1 tag size is determined as 74 bps INFO @ Tue, 02 Aug 2022 15:30:47: #1 tag size = 74 INFO @ Tue, 02 Aug 2022 15:30:47: #1 total tags in treatment: 31257408 INFO @ Tue, 02 Aug 2022 15:30:47: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 15:30:47: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 15:30:48: #1 tags after filtering in treatment: 31257408 INFO @ Tue, 02 Aug 2022 15:30:48: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 15:30:48: #1 finished! INFO @ Tue, 02 Aug 2022 15:30:48: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 15:30:48: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 15:30:50: #2 number of paired peaks: 3 WARNING @ Tue, 02 Aug 2022 15:30:50: Too few paired peaks (3) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 15:30:50: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX13227897/SRX13227897.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13227897/SRX13227897.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13227897/SRX13227897.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13227897/SRX13227897.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 15:30:51: 29000000 INFO @ Tue, 02 Aug 2022 15:30:55: 26000000 INFO @ Tue, 02 Aug 2022 15:30:59: 30000000 INFO @ Tue, 02 Aug 2022 15:31:03: 27000000 INFO @ Tue, 02 Aug 2022 15:31:07: 31000000 INFO @ Tue, 02 Aug 2022 15:31:09: #1 tag size is determined as 74 bps INFO @ Tue, 02 Aug 2022 15:31:09: #1 tag size = 74 INFO @ Tue, 02 Aug 2022 15:31:09: #1 total tags in treatment: 31257408 INFO @ Tue, 02 Aug 2022 15:31:09: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 15:31:09: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 15:31:10: #1 tags after filtering in treatment: 31257408 INFO @ Tue, 02 Aug 2022 15:31:10: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 15:31:10: #1 finished! INFO @ Tue, 02 Aug 2022 15:31:10: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 15:31:10: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 15:31:11: 28000000 INFO @ Tue, 02 Aug 2022 15:31:12: #2 number of paired peaks: 3 WARNING @ Tue, 02 Aug 2022 15:31:12: Too few paired peaks (3) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 15:31:12: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX13227897/SRX13227897.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13227897/SRX13227897.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13227897/SRX13227897.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13227897/SRX13227897.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 15:31:20: 29000000 INFO @ Tue, 02 Aug 2022 15:31:28: 30000000 INFO @ Tue, 02 Aug 2022 15:31:36: 31000000 INFO @ Tue, 02 Aug 2022 15:31:38: #1 tag size is determined as 74 bps INFO @ Tue, 02 Aug 2022 15:31:38: #1 tag size = 74 INFO @ Tue, 02 Aug 2022 15:31:38: #1 total tags in treatment: 31257408 INFO @ Tue, 02 Aug 2022 15:31:38: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 15:31:38: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 15:31:39: #1 tags after filtering in treatment: 31257408 INFO @ Tue, 02 Aug 2022 15:31:39: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 15:31:39: #1 finished! INFO @ Tue, 02 Aug 2022 15:31:39: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 15:31:39: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 15:31:41: #2 number of paired peaks: 3 WARNING @ Tue, 02 Aug 2022 15:31:41: Too few paired peaks (3) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 15:31:41: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX13227897/SRX13227897.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13227897/SRX13227897.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13227897/SRX13227897.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13227897/SRX13227897.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。