Job ID = 16439337 SRX = SRX13227895 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 35768049 spots for SRR17038008/SRR17038008.sra Written 35768049 spots for SRR17038008/SRR17038008.sra fastq に変換しました。 bowtie でマッピング中... Your job 16439466 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:15:36 35768049 reads; of these: 35768049 (100.00%) were unpaired; of these: 1051865 (2.94%) aligned 0 times 25614389 (71.61%) aligned exactly 1 time 9101795 (25.45%) aligned >1 times 97.06% overall alignment rate Time searching: 00:15:36 Overall time: 00:15:36 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 3999464 / 34716184 = 0.1152 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 15:22:17: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX13227895/SRX13227895.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX13227895/SRX13227895.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX13227895/SRX13227895.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX13227895/SRX13227895.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 15:22:17: #1 read tag files... INFO @ Tue, 02 Aug 2022 15:22:17: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 15:22:23: 1000000 INFO @ Tue, 02 Aug 2022 15:22:29: 2000000 INFO @ Tue, 02 Aug 2022 15:22:35: 3000000 INFO @ Tue, 02 Aug 2022 15:22:41: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 15:22:46: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX13227895/SRX13227895.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX13227895/SRX13227895.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX13227895/SRX13227895.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX13227895/SRX13227895.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 15:22:46: #1 read tag files... INFO @ Tue, 02 Aug 2022 15:22:46: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 15:22:47: 5000000 INFO @ Tue, 02 Aug 2022 15:22:53: 1000000 INFO @ Tue, 02 Aug 2022 15:22:54: 6000000 INFO @ Tue, 02 Aug 2022 15:23:00: 2000000 INFO @ Tue, 02 Aug 2022 15:23:00: 7000000 INFO @ Tue, 02 Aug 2022 15:23:06: 3000000 INFO @ Tue, 02 Aug 2022 15:23:07: 8000000 INFO @ Tue, 02 Aug 2022 15:23:13: 4000000 INFO @ Tue, 02 Aug 2022 15:23:13: 9000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 15:23:16: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX13227895/SRX13227895.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX13227895/SRX13227895.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX13227895/SRX13227895.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX13227895/SRX13227895.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 15:23:16: #1 read tag files... INFO @ Tue, 02 Aug 2022 15:23:16: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 15:23:19: 5000000 INFO @ Tue, 02 Aug 2022 15:23:20: 10000000 INFO @ Tue, 02 Aug 2022 15:23:23: 1000000 INFO @ Tue, 02 Aug 2022 15:23:26: 6000000 INFO @ Tue, 02 Aug 2022 15:23:27: 11000000 INFO @ Tue, 02 Aug 2022 15:23:29: 2000000 INFO @ Tue, 02 Aug 2022 15:23:33: 7000000 INFO @ Tue, 02 Aug 2022 15:23:33: 12000000 INFO @ Tue, 02 Aug 2022 15:23:36: 3000000 INFO @ Tue, 02 Aug 2022 15:23:39: 8000000 INFO @ Tue, 02 Aug 2022 15:23:40: 13000000 INFO @ Tue, 02 Aug 2022 15:23:43: 4000000 INFO @ Tue, 02 Aug 2022 15:23:46: 9000000 INFO @ Tue, 02 Aug 2022 15:23:47: 14000000 INFO @ Tue, 02 Aug 2022 15:23:49: 5000000 INFO @ Tue, 02 Aug 2022 15:23:52: 10000000 INFO @ Tue, 02 Aug 2022 15:23:53: 15000000 INFO @ Tue, 02 Aug 2022 15:23:56: 6000000 INFO @ Tue, 02 Aug 2022 15:23:59: 11000000 INFO @ Tue, 02 Aug 2022 15:24:00: 16000000 INFO @ Tue, 02 Aug 2022 15:24:02: 7000000 INFO @ Tue, 02 Aug 2022 15:24:05: 12000000 INFO @ Tue, 02 Aug 2022 15:24:06: 17000000 INFO @ Tue, 02 Aug 2022 15:24:09: 8000000 INFO @ Tue, 02 Aug 2022 15:24:12: 13000000 INFO @ Tue, 02 Aug 2022 15:24:13: 18000000 INFO @ Tue, 02 Aug 2022 15:24:15: 9000000 INFO @ Tue, 02 Aug 2022 15:24:18: 14000000 INFO @ Tue, 02 Aug 2022 15:24:19: 19000000 INFO @ Tue, 02 Aug 2022 15:24:21: 10000000 INFO @ Tue, 02 Aug 2022 15:24:25: 15000000 INFO @ Tue, 02 Aug 2022 15:24:26: 20000000 INFO @ Tue, 02 Aug 2022 15:24:28: 11000000 INFO @ Tue, 02 Aug 2022 15:24:32: 16000000 INFO @ Tue, 02 Aug 2022 15:24:32: 21000000 INFO @ Tue, 02 Aug 2022 15:24:34: 12000000 INFO @ Tue, 02 Aug 2022 15:24:38: 17000000 INFO @ Tue, 02 Aug 2022 15:24:39: 22000000 INFO @ Tue, 02 Aug 2022 15:24:41: 13000000 INFO @ Tue, 02 Aug 2022 15:24:44: 18000000 INFO @ Tue, 02 Aug 2022 15:24:46: 23000000 INFO @ Tue, 02 Aug 2022 15:24:47: 14000000 INFO @ Tue, 02 Aug 2022 15:24:51: 19000000 INFO @ Tue, 02 Aug 2022 15:24:52: 24000000 INFO @ Tue, 02 Aug 2022 15:24:54: 15000000 INFO @ Tue, 02 Aug 2022 15:24:57: 20000000 INFO @ Tue, 02 Aug 2022 15:24:59: 25000000 INFO @ Tue, 02 Aug 2022 15:25:00: 16000000 INFO @ Tue, 02 Aug 2022 15:25:04: 21000000 INFO @ Tue, 02 Aug 2022 15:25:05: 26000000 INFO @ Tue, 02 Aug 2022 15:25:07: 17000000 INFO @ Tue, 02 Aug 2022 15:25:11: 22000000 INFO @ Tue, 02 Aug 2022 15:25:12: 27000000 INFO @ Tue, 02 Aug 2022 15:25:14: 18000000 INFO @ Tue, 02 Aug 2022 15:25:17: 23000000 INFO @ Tue, 02 Aug 2022 15:25:19: 28000000 INFO @ Tue, 02 Aug 2022 15:25:20: 19000000 INFO @ Tue, 02 Aug 2022 15:25:24: 24000000 INFO @ Tue, 02 Aug 2022 15:25:26: 29000000 INFO @ Tue, 02 Aug 2022 15:25:27: 20000000 INFO @ Tue, 02 Aug 2022 15:25:30: 25000000 INFO @ Tue, 02 Aug 2022 15:25:32: 30000000 INFO @ Tue, 02 Aug 2022 15:25:33: 21000000 INFO @ Tue, 02 Aug 2022 15:25:37: 26000000 INFO @ Tue, 02 Aug 2022 15:25:37: #1 tag size is determined as 74 bps INFO @ Tue, 02 Aug 2022 15:25:37: #1 tag size = 74 INFO @ Tue, 02 Aug 2022 15:25:37: #1 total tags in treatment: 30716720 INFO @ Tue, 02 Aug 2022 15:25:37: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 15:25:37: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 15:25:38: #1 tags after filtering in treatment: 30716720 INFO @ Tue, 02 Aug 2022 15:25:38: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 15:25:38: #1 finished! INFO @ Tue, 02 Aug 2022 15:25:38: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 15:25:38: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 15:25:40: 22000000 INFO @ Tue, 02 Aug 2022 15:25:40: #2 number of paired peaks: 4 WARNING @ Tue, 02 Aug 2022 15:25:40: Too few paired peaks (4) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 15:25:40: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX13227895/SRX13227895.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13227895/SRX13227895.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13227895/SRX13227895.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13227895/SRX13227895.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 15:25:44: 27000000 INFO @ Tue, 02 Aug 2022 15:25:46: 23000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 02 Aug 2022 15:25:50: 28000000 INFO @ Tue, 02 Aug 2022 15:25:53: 24000000 INFO @ Tue, 02 Aug 2022 15:25:57: 29000000 INFO @ Tue, 02 Aug 2022 15:26:00: 25000000 INFO @ Tue, 02 Aug 2022 15:26:04: 30000000 INFO @ Tue, 02 Aug 2022 15:26:06: 26000000 INFO @ Tue, 02 Aug 2022 15:26:09: #1 tag size is determined as 74 bps INFO @ Tue, 02 Aug 2022 15:26:09: #1 tag size = 74 INFO @ Tue, 02 Aug 2022 15:26:09: #1 total tags in treatment: 30716720 INFO @ Tue, 02 Aug 2022 15:26:09: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 15:26:09: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 15:26:10: #1 tags after filtering in treatment: 30716720 INFO @ Tue, 02 Aug 2022 15:26:10: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 15:26:10: #1 finished! INFO @ Tue, 02 Aug 2022 15:26:10: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 15:26:10: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 15:26:12: #2 number of paired peaks: 4 WARNING @ Tue, 02 Aug 2022 15:26:12: Too few paired peaks (4) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 15:26:12: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX13227895/SRX13227895.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13227895/SRX13227895.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13227895/SRX13227895.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13227895/SRX13227895.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 15:26:12: 27000000 INFO @ Tue, 02 Aug 2022 15:26:20: 28000000 INFO @ Tue, 02 Aug 2022 15:26:26: 29000000 INFO @ Tue, 02 Aug 2022 15:26:32: 30000000 INFO @ Tue, 02 Aug 2022 15:26:37: #1 tag size is determined as 74 bps INFO @ Tue, 02 Aug 2022 15:26:37: #1 tag size = 74 INFO @ Tue, 02 Aug 2022 15:26:37: #1 total tags in treatment: 30716720 INFO @ Tue, 02 Aug 2022 15:26:37: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 15:26:37: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 15:26:37: #1 tags after filtering in treatment: 30716720 INFO @ Tue, 02 Aug 2022 15:26:37: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 15:26:37: #1 finished! INFO @ Tue, 02 Aug 2022 15:26:37: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 15:26:37: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 15:26:39: #2 number of paired peaks: 4 WARNING @ Tue, 02 Aug 2022 15:26:39: Too few paired peaks (4) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 15:26:39: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX13227895/SRX13227895.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13227895/SRX13227895.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13227895/SRX13227895.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13227895/SRX13227895.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。