Job ID = 16439336 SRX = SRX13227894 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 36266588 spots for SRR17038007/SRR17038007.sra Written 36266588 spots for SRR17038007/SRR17038007.sra fastq に変換しました。 bowtie でマッピング中... Your job 16439511 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:22:39 36266588 reads; of these: 36266588 (100.00%) were unpaired; of these: 1033533 (2.85%) aligned 0 times 24908260 (68.68%) aligned exactly 1 time 10324795 (28.47%) aligned >1 times 97.15% overall alignment rate Time searching: 00:22:39 Overall time: 00:22:39 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 4486036 / 35233055 = 0.1273 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 15:33:55: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX13227894/SRX13227894.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX13227894/SRX13227894.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX13227894/SRX13227894.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX13227894/SRX13227894.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 15:33:55: #1 read tag files... INFO @ Tue, 02 Aug 2022 15:33:55: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 15:34:07: 1000000 INFO @ Tue, 02 Aug 2022 15:34:19: 2000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 15:34:25: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX13227894/SRX13227894.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX13227894/SRX13227894.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX13227894/SRX13227894.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX13227894/SRX13227894.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 15:34:25: #1 read tag files... INFO @ Tue, 02 Aug 2022 15:34:25: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 15:34:31: 3000000 INFO @ Tue, 02 Aug 2022 15:34:36: 1000000 INFO @ Tue, 02 Aug 2022 15:34:44: 4000000 INFO @ Tue, 02 Aug 2022 15:34:48: 2000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 15:34:55: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX13227894/SRX13227894.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX13227894/SRX13227894.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX13227894/SRX13227894.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX13227894/SRX13227894.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 15:34:55: #1 read tag files... INFO @ Tue, 02 Aug 2022 15:34:55: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 15:34:56: 5000000 INFO @ Tue, 02 Aug 2022 15:34:59: 3000000 INFO @ Tue, 02 Aug 2022 15:35:06: 1000000 INFO @ Tue, 02 Aug 2022 15:35:09: 6000000 INFO @ Tue, 02 Aug 2022 15:35:11: 4000000 INFO @ Tue, 02 Aug 2022 15:35:16: 2000000 INFO @ Tue, 02 Aug 2022 15:35:21: 7000000 INFO @ Tue, 02 Aug 2022 15:35:23: 5000000 INFO @ Tue, 02 Aug 2022 15:35:27: 3000000 INFO @ Tue, 02 Aug 2022 15:35:33: 8000000 INFO @ Tue, 02 Aug 2022 15:35:34: 6000000 INFO @ Tue, 02 Aug 2022 15:35:37: 4000000 INFO @ Tue, 02 Aug 2022 15:35:45: 9000000 INFO @ Tue, 02 Aug 2022 15:35:46: 7000000 INFO @ Tue, 02 Aug 2022 15:35:47: 5000000 INFO @ Tue, 02 Aug 2022 15:35:56: 10000000 INFO @ Tue, 02 Aug 2022 15:35:57: 8000000 INFO @ Tue, 02 Aug 2022 15:35:58: 6000000 INFO @ Tue, 02 Aug 2022 15:36:08: 7000000 INFO @ Tue, 02 Aug 2022 15:36:08: 9000000 INFO @ Tue, 02 Aug 2022 15:36:08: 11000000 INFO @ Tue, 02 Aug 2022 15:36:18: 8000000 INFO @ Tue, 02 Aug 2022 15:36:19: 10000000 INFO @ Tue, 02 Aug 2022 15:36:20: 12000000 INFO @ Tue, 02 Aug 2022 15:36:29: 9000000 INFO @ Tue, 02 Aug 2022 15:36:31: 11000000 INFO @ Tue, 02 Aug 2022 15:36:33: 13000000 INFO @ Tue, 02 Aug 2022 15:36:39: 10000000 INFO @ Tue, 02 Aug 2022 15:36:43: 12000000 INFO @ Tue, 02 Aug 2022 15:36:46: 14000000 INFO @ Tue, 02 Aug 2022 15:36:50: 11000000 INFO @ Tue, 02 Aug 2022 15:36:55: 13000000 INFO @ Tue, 02 Aug 2022 15:36:58: 15000000 INFO @ Tue, 02 Aug 2022 15:37:00: 12000000 INFO @ Tue, 02 Aug 2022 15:37:06: 14000000 INFO @ Tue, 02 Aug 2022 15:37:10: 16000000 INFO @ Tue, 02 Aug 2022 15:37:11: 13000000 INFO @ Tue, 02 Aug 2022 15:37:18: 15000000 INFO @ Tue, 02 Aug 2022 15:37:21: 14000000 INFO @ Tue, 02 Aug 2022 15:37:22: 17000000 INFO @ Tue, 02 Aug 2022 15:37:29: 16000000 INFO @ Tue, 02 Aug 2022 15:37:32: 15000000 INFO @ Tue, 02 Aug 2022 15:37:33: 18000000 INFO @ Tue, 02 Aug 2022 15:37:40: 17000000 INFO @ Tue, 02 Aug 2022 15:37:42: 16000000 INFO @ Tue, 02 Aug 2022 15:37:44: 19000000 INFO @ Tue, 02 Aug 2022 15:37:51: 18000000 INFO @ Tue, 02 Aug 2022 15:37:53: 17000000 INFO @ Tue, 02 Aug 2022 15:37:56: 20000000 INFO @ Tue, 02 Aug 2022 15:38:03: 19000000 INFO @ Tue, 02 Aug 2022 15:38:03: 18000000 INFO @ Tue, 02 Aug 2022 15:38:07: 21000000 INFO @ Tue, 02 Aug 2022 15:38:13: 19000000 INFO @ Tue, 02 Aug 2022 15:38:14: 20000000 INFO @ Tue, 02 Aug 2022 15:38:18: 22000000 INFO @ Tue, 02 Aug 2022 15:38:24: 20000000 INFO @ Tue, 02 Aug 2022 15:38:25: 21000000 INFO @ Tue, 02 Aug 2022 15:38:30: 23000000 INFO @ Tue, 02 Aug 2022 15:38:34: 21000000 INFO @ Tue, 02 Aug 2022 15:38:36: 22000000 INFO @ Tue, 02 Aug 2022 15:38:42: 24000000 INFO @ Tue, 02 Aug 2022 15:38:45: 22000000 INFO @ Tue, 02 Aug 2022 15:38:48: 23000000 INFO @ Tue, 02 Aug 2022 15:38:53: 25000000 INFO @ Tue, 02 Aug 2022 15:38:55: 23000000 INFO @ Tue, 02 Aug 2022 15:38:59: 24000000 INFO @ Tue, 02 Aug 2022 15:39:04: 26000000 INFO @ Tue, 02 Aug 2022 15:39:06: 24000000 INFO @ Tue, 02 Aug 2022 15:39:10: 25000000 INFO @ Tue, 02 Aug 2022 15:39:16: 25000000 INFO @ Tue, 02 Aug 2022 15:39:16: 27000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 02 Aug 2022 15:39:22: 26000000 INFO @ Tue, 02 Aug 2022 15:39:26: 26000000 INFO @ Tue, 02 Aug 2022 15:39:27: 28000000 INFO @ Tue, 02 Aug 2022 15:39:33: 27000000 INFO @ Tue, 02 Aug 2022 15:39:37: 27000000 INFO @ Tue, 02 Aug 2022 15:39:38: 29000000 INFO @ Tue, 02 Aug 2022 15:39:44: 28000000 INFO @ Tue, 02 Aug 2022 15:39:47: 28000000 INFO @ Tue, 02 Aug 2022 15:39:49: 30000000 INFO @ Tue, 02 Aug 2022 15:39:56: 29000000 INFO @ Tue, 02 Aug 2022 15:39:57: 29000000 INFO @ Tue, 02 Aug 2022 15:39:58: #1 tag size is determined as 74 bps INFO @ Tue, 02 Aug 2022 15:39:58: #1 tag size = 74 INFO @ Tue, 02 Aug 2022 15:39:58: #1 total tags in treatment: 30747019 INFO @ Tue, 02 Aug 2022 15:39:58: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 15:39:58: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 15:39:58: #1 tags after filtering in treatment: 30747019 INFO @ Tue, 02 Aug 2022 15:39:58: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 15:39:58: #1 finished! INFO @ Tue, 02 Aug 2022 15:39:58: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 15:39:58: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 15:40:01: #2 number of paired peaks: 58 WARNING @ Tue, 02 Aug 2022 15:40:01: Too few paired peaks (58) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 15:40:01: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX13227894/SRX13227894.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13227894/SRX13227894.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13227894/SRX13227894.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13227894/SRX13227894.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 15:40:06: 30000000 INFO @ Tue, 02 Aug 2022 15:40:07: 30000000 INFO @ Tue, 02 Aug 2022 15:40:15: #1 tag size is determined as 74 bps INFO @ Tue, 02 Aug 2022 15:40:15: #1 tag size = 74 INFO @ Tue, 02 Aug 2022 15:40:15: #1 total tags in treatment: 30747019 INFO @ Tue, 02 Aug 2022 15:40:15: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 15:40:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 15:40:15: #1 tag size is determined as 74 bps INFO @ Tue, 02 Aug 2022 15:40:15: #1 tag size = 74 INFO @ Tue, 02 Aug 2022 15:40:15: #1 total tags in treatment: 30747019 INFO @ Tue, 02 Aug 2022 15:40:15: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 15:40:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 15:40:16: #1 tags after filtering in treatment: 30747019 INFO @ Tue, 02 Aug 2022 15:40:16: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 15:40:16: #1 finished! INFO @ Tue, 02 Aug 2022 15:40:16: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 15:40:16: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 15:40:16: #1 tags after filtering in treatment: 30747019 INFO @ Tue, 02 Aug 2022 15:40:16: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 15:40:16: #1 finished! INFO @ Tue, 02 Aug 2022 15:40:16: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 15:40:16: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 15:40:18: #2 number of paired peaks: 58 WARNING @ Tue, 02 Aug 2022 15:40:18: Too few paired peaks (58) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 15:40:18: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX13227894/SRX13227894.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13227894/SRX13227894.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13227894/SRX13227894.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13227894/SRX13227894.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 15:40:19: #2 number of paired peaks: 58 WARNING @ Tue, 02 Aug 2022 15:40:19: Too few paired peaks (58) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 15:40:19: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX13227894/SRX13227894.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13227894/SRX13227894.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13227894/SRX13227894.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13227894/SRX13227894.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。