Job ID = 16439334 SRX = SRX13227892 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 30236897 spots for SRR17038005/SRR17038005.sra Written 30236897 spots for SRR17038005/SRR17038005.sra fastq に変換しました。 bowtie でマッピング中... Your job 16439452 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:13:12 30236897 reads; of these: 30236897 (100.00%) were unpaired; of these: 720961 (2.38%) aligned 0 times 20683187 (68.40%) aligned exactly 1 time 8832749 (29.21%) aligned >1 times 97.62% overall alignment rate Time searching: 00:13:12 Overall time: 00:13:12 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 3405762 / 29515936 = 0.1154 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 15:17:59: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX13227892/SRX13227892.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX13227892/SRX13227892.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX13227892/SRX13227892.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX13227892/SRX13227892.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 15:17:59: #1 read tag files... INFO @ Tue, 02 Aug 2022 15:17:59: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 15:18:06: 1000000 INFO @ Tue, 02 Aug 2022 15:18:13: 2000000 INFO @ Tue, 02 Aug 2022 15:18:20: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 15:18:27: 4000000 INFO @ Tue, 02 Aug 2022 15:18:28: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX13227892/SRX13227892.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX13227892/SRX13227892.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX13227892/SRX13227892.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX13227892/SRX13227892.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 15:18:28: #1 read tag files... INFO @ Tue, 02 Aug 2022 15:18:28: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 15:18:34: 5000000 INFO @ Tue, 02 Aug 2022 15:18:35: 1000000 INFO @ Tue, 02 Aug 2022 15:18:42: 6000000 INFO @ Tue, 02 Aug 2022 15:18:43: 2000000 INFO @ Tue, 02 Aug 2022 15:18:50: 7000000 INFO @ Tue, 02 Aug 2022 15:18:51: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 15:18:58: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX13227892/SRX13227892.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX13227892/SRX13227892.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX13227892/SRX13227892.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX13227892/SRX13227892.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 15:18:58: #1 read tag files... INFO @ Tue, 02 Aug 2022 15:18:58: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 15:18:58: 4000000 INFO @ Tue, 02 Aug 2022 15:18:59: 8000000 INFO @ Tue, 02 Aug 2022 15:19:07: 1000000 INFO @ Tue, 02 Aug 2022 15:19:07: 5000000 INFO @ Tue, 02 Aug 2022 15:19:07: 9000000 INFO @ Tue, 02 Aug 2022 15:19:15: 6000000 INFO @ Tue, 02 Aug 2022 15:19:15: 2000000 INFO @ Tue, 02 Aug 2022 15:19:16: 10000000 INFO @ Tue, 02 Aug 2022 15:19:24: 7000000 INFO @ Tue, 02 Aug 2022 15:19:24: 3000000 INFO @ Tue, 02 Aug 2022 15:19:25: 11000000 INFO @ Tue, 02 Aug 2022 15:19:32: 8000000 INFO @ Tue, 02 Aug 2022 15:19:33: 4000000 INFO @ Tue, 02 Aug 2022 15:19:34: 12000000 INFO @ Tue, 02 Aug 2022 15:19:41: 9000000 INFO @ Tue, 02 Aug 2022 15:19:41: 5000000 INFO @ Tue, 02 Aug 2022 15:19:43: 13000000 INFO @ Tue, 02 Aug 2022 15:19:50: 10000000 INFO @ Tue, 02 Aug 2022 15:19:50: 6000000 INFO @ Tue, 02 Aug 2022 15:19:52: 14000000 INFO @ Tue, 02 Aug 2022 15:19:58: 11000000 INFO @ Tue, 02 Aug 2022 15:19:59: 7000000 INFO @ Tue, 02 Aug 2022 15:20:01: 15000000 INFO @ Tue, 02 Aug 2022 15:20:06: 12000000 INFO @ Tue, 02 Aug 2022 15:20:08: 8000000 INFO @ Tue, 02 Aug 2022 15:20:10: 16000000 INFO @ Tue, 02 Aug 2022 15:20:15: 13000000 INFO @ Tue, 02 Aug 2022 15:20:16: 9000000 INFO @ Tue, 02 Aug 2022 15:20:18: 17000000 INFO @ Tue, 02 Aug 2022 15:20:23: 14000000 INFO @ Tue, 02 Aug 2022 15:20:25: 10000000 INFO @ Tue, 02 Aug 2022 15:20:27: 18000000 INFO @ Tue, 02 Aug 2022 15:20:32: 15000000 INFO @ Tue, 02 Aug 2022 15:20:34: 11000000 INFO @ Tue, 02 Aug 2022 15:20:36: 19000000 INFO @ Tue, 02 Aug 2022 15:20:40: 16000000 INFO @ Tue, 02 Aug 2022 15:20:43: 12000000 INFO @ Tue, 02 Aug 2022 15:20:45: 20000000 INFO @ Tue, 02 Aug 2022 15:20:49: 17000000 INFO @ Tue, 02 Aug 2022 15:20:52: 13000000 INFO @ Tue, 02 Aug 2022 15:20:54: 21000000 INFO @ Tue, 02 Aug 2022 15:20:58: 18000000 INFO @ Tue, 02 Aug 2022 15:21:00: 14000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 02 Aug 2022 15:21:03: 22000000 INFO @ Tue, 02 Aug 2022 15:21:06: 19000000 INFO @ Tue, 02 Aug 2022 15:21:09: 15000000 INFO @ Tue, 02 Aug 2022 15:21:12: 23000000 INFO @ Tue, 02 Aug 2022 15:21:15: 20000000 INFO @ Tue, 02 Aug 2022 15:21:17: 16000000 INFO @ Tue, 02 Aug 2022 15:21:21: 24000000 INFO @ Tue, 02 Aug 2022 15:21:24: 21000000 INFO @ Tue, 02 Aug 2022 15:21:25: 17000000 INFO @ Tue, 02 Aug 2022 15:21:30: 25000000 INFO @ Tue, 02 Aug 2022 15:21:32: 22000000 INFO @ Tue, 02 Aug 2022 15:21:33: 18000000 INFO @ Tue, 02 Aug 2022 15:21:38: 26000000 INFO @ Tue, 02 Aug 2022 15:21:39: #1 tag size is determined as 74 bps INFO @ Tue, 02 Aug 2022 15:21:39: #1 tag size = 74 INFO @ Tue, 02 Aug 2022 15:21:39: #1 total tags in treatment: 26110174 INFO @ Tue, 02 Aug 2022 15:21:39: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 15:21:39: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 15:21:40: #1 tags after filtering in treatment: 26110174 INFO @ Tue, 02 Aug 2022 15:21:40: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 15:21:40: #1 finished! INFO @ Tue, 02 Aug 2022 15:21:40: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 15:21:40: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 15:21:41: 23000000 INFO @ Tue, 02 Aug 2022 15:21:41: 19000000 INFO @ Tue, 02 Aug 2022 15:21:42: #2 number of paired peaks: 56 WARNING @ Tue, 02 Aug 2022 15:21:42: Too few paired peaks (56) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 15:21:42: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX13227892/SRX13227892.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13227892/SRX13227892.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13227892/SRX13227892.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13227892/SRX13227892.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 15:21:50: 20000000 INFO @ Tue, 02 Aug 2022 15:21:50: 24000000 INFO @ Tue, 02 Aug 2022 15:21:58: 21000000 INFO @ Tue, 02 Aug 2022 15:21:59: 25000000 BigWig に変換しました。 INFO @ Tue, 02 Aug 2022 15:22:06: 22000000 INFO @ Tue, 02 Aug 2022 15:22:07: 26000000 INFO @ Tue, 02 Aug 2022 15:22:08: #1 tag size is determined as 74 bps INFO @ Tue, 02 Aug 2022 15:22:08: #1 tag size = 74 INFO @ Tue, 02 Aug 2022 15:22:08: #1 total tags in treatment: 26110174 INFO @ Tue, 02 Aug 2022 15:22:08: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 15:22:08: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 15:22:09: #1 tags after filtering in treatment: 26110174 INFO @ Tue, 02 Aug 2022 15:22:09: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 15:22:09: #1 finished! INFO @ Tue, 02 Aug 2022 15:22:09: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 15:22:09: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 15:22:10: #2 number of paired peaks: 56 WARNING @ Tue, 02 Aug 2022 15:22:10: Too few paired peaks (56) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 15:22:10: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX13227892/SRX13227892.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13227892/SRX13227892.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13227892/SRX13227892.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13227892/SRX13227892.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 15:22:13: 23000000 INFO @ Tue, 02 Aug 2022 15:22:21: 24000000 INFO @ Tue, 02 Aug 2022 15:22:28: 25000000 INFO @ Tue, 02 Aug 2022 15:22:35: 26000000 INFO @ Tue, 02 Aug 2022 15:22:36: #1 tag size is determined as 74 bps INFO @ Tue, 02 Aug 2022 15:22:36: #1 tag size = 74 INFO @ Tue, 02 Aug 2022 15:22:36: #1 total tags in treatment: 26110174 INFO @ Tue, 02 Aug 2022 15:22:36: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 15:22:36: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 15:22:37: #1 tags after filtering in treatment: 26110174 INFO @ Tue, 02 Aug 2022 15:22:37: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 15:22:37: #1 finished! INFO @ Tue, 02 Aug 2022 15:22:37: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 15:22:37: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 15:22:38: #2 number of paired peaks: 56 WARNING @ Tue, 02 Aug 2022 15:22:38: Too few paired peaks (56) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 15:22:38: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX13227892/SRX13227892.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13227892/SRX13227892.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13227892/SRX13227892.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13227892/SRX13227892.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling