Job ID = 16439332 SRX = SRX13227890 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 35948242 spots for SRR17038003/SRR17038003.sra Written 35948242 spots for SRR17038003/SRR17038003.sra fastq に変換しました。 bowtie でマッピング中... Your job 16439477 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:17:26 35948242 reads; of these: 35948242 (100.00%) were unpaired; of these: 908807 (2.53%) aligned 0 times 24847830 (69.12%) aligned exactly 1 time 10191605 (28.35%) aligned >1 times 97.47% overall alignment rate Time searching: 00:17:26 Overall time: 00:17:26 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 4577450 / 35039435 = 0.1306 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 15:26:24: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX13227890/SRX13227890.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX13227890/SRX13227890.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX13227890/SRX13227890.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX13227890/SRX13227890.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 15:26:24: #1 read tag files... INFO @ Tue, 02 Aug 2022 15:26:24: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 15:26:33: 1000000 INFO @ Tue, 02 Aug 2022 15:26:43: 2000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 15:26:52: 3000000 INFO @ Tue, 02 Aug 2022 15:26:55: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX13227890/SRX13227890.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX13227890/SRX13227890.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX13227890/SRX13227890.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX13227890/SRX13227890.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 15:26:55: #1 read tag files... INFO @ Tue, 02 Aug 2022 15:26:55: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 15:27:02: 4000000 INFO @ Tue, 02 Aug 2022 15:27:05: 1000000 INFO @ Tue, 02 Aug 2022 15:27:12: 5000000 INFO @ Tue, 02 Aug 2022 15:27:14: 2000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 15:27:22: 6000000 INFO @ Tue, 02 Aug 2022 15:27:23: 3000000 INFO @ Tue, 02 Aug 2022 15:27:24: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX13227890/SRX13227890.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX13227890/SRX13227890.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX13227890/SRX13227890.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX13227890/SRX13227890.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 15:27:24: #1 read tag files... INFO @ Tue, 02 Aug 2022 15:27:24: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 15:27:32: 7000000 INFO @ Tue, 02 Aug 2022 15:27:32: 1000000 INFO @ Tue, 02 Aug 2022 15:27:33: 4000000 INFO @ Tue, 02 Aug 2022 15:27:41: 2000000 INFO @ Tue, 02 Aug 2022 15:27:42: 8000000 INFO @ Tue, 02 Aug 2022 15:27:42: 5000000 INFO @ Tue, 02 Aug 2022 15:27:49: 3000000 INFO @ Tue, 02 Aug 2022 15:27:51: 6000000 INFO @ Tue, 02 Aug 2022 15:27:51: 9000000 INFO @ Tue, 02 Aug 2022 15:27:57: 4000000 INFO @ Tue, 02 Aug 2022 15:28:01: 7000000 INFO @ Tue, 02 Aug 2022 15:28:01: 10000000 INFO @ Tue, 02 Aug 2022 15:28:06: 5000000 INFO @ Tue, 02 Aug 2022 15:28:10: 8000000 INFO @ Tue, 02 Aug 2022 15:28:11: 11000000 INFO @ Tue, 02 Aug 2022 15:28:14: 6000000 INFO @ Tue, 02 Aug 2022 15:28:20: 9000000 INFO @ Tue, 02 Aug 2022 15:28:22: 12000000 INFO @ Tue, 02 Aug 2022 15:28:22: 7000000 INFO @ Tue, 02 Aug 2022 15:28:29: 10000000 INFO @ Tue, 02 Aug 2022 15:28:32: 13000000 INFO @ Tue, 02 Aug 2022 15:28:33: 8000000 INFO @ Tue, 02 Aug 2022 15:28:39: 11000000 INFO @ Tue, 02 Aug 2022 15:28:42: 14000000 INFO @ Tue, 02 Aug 2022 15:28:44: 9000000 INFO @ Tue, 02 Aug 2022 15:28:48: 12000000 INFO @ Tue, 02 Aug 2022 15:28:52: 15000000 INFO @ Tue, 02 Aug 2022 15:28:53: 10000000 INFO @ Tue, 02 Aug 2022 15:28:58: 13000000 INFO @ Tue, 02 Aug 2022 15:29:01: 11000000 INFO @ Tue, 02 Aug 2022 15:29:02: 16000000 INFO @ Tue, 02 Aug 2022 15:29:07: 14000000 INFO @ Tue, 02 Aug 2022 15:29:10: 12000000 INFO @ Tue, 02 Aug 2022 15:29:12: 17000000 INFO @ Tue, 02 Aug 2022 15:29:16: 15000000 INFO @ Tue, 02 Aug 2022 15:29:19: 13000000 INFO @ Tue, 02 Aug 2022 15:29:21: 18000000 INFO @ Tue, 02 Aug 2022 15:29:26: 16000000 INFO @ Tue, 02 Aug 2022 15:29:27: 14000000 INFO @ Tue, 02 Aug 2022 15:29:32: 19000000 INFO @ Tue, 02 Aug 2022 15:29:35: 17000000 INFO @ Tue, 02 Aug 2022 15:29:35: 15000000 INFO @ Tue, 02 Aug 2022 15:29:41: 20000000 INFO @ Tue, 02 Aug 2022 15:29:43: 16000000 INFO @ Tue, 02 Aug 2022 15:29:44: 18000000 INFO @ Tue, 02 Aug 2022 15:29:51: 17000000 INFO @ Tue, 02 Aug 2022 15:29:51: 21000000 INFO @ Tue, 02 Aug 2022 15:29:53: 19000000 INFO @ Tue, 02 Aug 2022 15:30:00: 18000000 INFO @ Tue, 02 Aug 2022 15:30:01: 22000000 INFO @ Tue, 02 Aug 2022 15:30:02: 20000000 INFO @ Tue, 02 Aug 2022 15:30:08: 19000000 INFO @ Tue, 02 Aug 2022 15:30:12: 21000000 INFO @ Tue, 02 Aug 2022 15:30:12: 23000000 INFO @ Tue, 02 Aug 2022 15:30:16: 20000000 INFO @ Tue, 02 Aug 2022 15:30:21: 22000000 INFO @ Tue, 02 Aug 2022 15:30:23: 24000000 INFO @ Tue, 02 Aug 2022 15:30:25: 21000000 INFO @ Tue, 02 Aug 2022 15:30:31: 23000000 INFO @ Tue, 02 Aug 2022 15:30:33: 22000000 INFO @ Tue, 02 Aug 2022 15:30:33: 25000000 INFO @ Tue, 02 Aug 2022 15:30:40: 24000000 INFO @ Tue, 02 Aug 2022 15:30:41: 23000000 INFO @ Tue, 02 Aug 2022 15:30:43: 26000000 INFO @ Tue, 02 Aug 2022 15:30:49: 24000000 INFO @ Tue, 02 Aug 2022 15:30:50: 25000000 INFO @ Tue, 02 Aug 2022 15:30:54: 27000000 INFO @ Tue, 02 Aug 2022 15:30:57: 25000000 INFO @ Tue, 02 Aug 2022 15:30:59: 26000000 INFO @ Tue, 02 Aug 2022 15:31:04: 28000000 INFO @ Tue, 02 Aug 2022 15:31:05: 26000000 INFO @ Tue, 02 Aug 2022 15:31:08: 27000000 INFO @ Tue, 02 Aug 2022 15:31:14: 27000000 INFO @ Tue, 02 Aug 2022 15:31:14: 29000000 INFO @ Tue, 02 Aug 2022 15:31:18: 28000000 INFO @ Tue, 02 Aug 2022 15:31:22: 28000000 INFO @ Tue, 02 Aug 2022 15:31:24: 30000000 INFO @ Tue, 02 Aug 2022 15:31:27: 29000000 INFO @ Tue, 02 Aug 2022 15:31:28: #1 tag size is determined as 74 bps INFO @ Tue, 02 Aug 2022 15:31:28: #1 tag size = 74 INFO @ Tue, 02 Aug 2022 15:31:28: #1 total tags in treatment: 30461985 INFO @ Tue, 02 Aug 2022 15:31:28: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 15:31:28: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 15:31:29: #1 tags after filtering in treatment: 30461985 INFO @ Tue, 02 Aug 2022 15:31:29: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 15:31:29: #1 finished! INFO @ Tue, 02 Aug 2022 15:31:29: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 15:31:29: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 15:31:30: 29000000 INFO @ Tue, 02 Aug 2022 15:31:31: #2 number of paired peaks: 50 WARNING @ Tue, 02 Aug 2022 15:31:31: Too few paired peaks (50) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 15:31:31: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX13227890/SRX13227890.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13227890/SRX13227890.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13227890/SRX13227890.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13227890/SRX13227890.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 15:31:36: 30000000 INFO @ Tue, 02 Aug 2022 15:31:39: 30000000 INFO @ Tue, 02 Aug 2022 15:31:40: #1 tag size is determined as 74 bps INFO @ Tue, 02 Aug 2022 15:31:40: #1 tag size = 74 INFO @ Tue, 02 Aug 2022 15:31:40: #1 total tags in treatment: 30461985 INFO @ Tue, 02 Aug 2022 15:31:40: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 15:31:40: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 15:31:41: #1 tags after filtering in treatment: 30461985 INFO @ Tue, 02 Aug 2022 15:31:41: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 15:31:41: #1 finished! INFO @ Tue, 02 Aug 2022 15:31:41: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 15:31:41: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 15:31:43: #2 number of paired peaks: 50 WARNING @ Tue, 02 Aug 2022 15:31:43: Too few paired peaks (50) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 15:31:43: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX13227890/SRX13227890.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis INFO @ Tue, 02 Aug 2022 15:31:43: #1 tag size is determined as 74 bps INFO @ Tue, 02 Aug 2022 15:31:43: #1 tag size = 74 INFO @ Tue, 02 Aug 2022 15:31:43: #1 total tags in treatment: 30461985 INFO @ Tue, 02 Aug 2022 15:31:43: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 15:31:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13227890/SRX13227890.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13227890/SRX13227890.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13227890/SRX13227890.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 15:31:44: #1 tags after filtering in treatment: 30461985 INFO @ Tue, 02 Aug 2022 15:31:44: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 15:31:44: #1 finished! INFO @ Tue, 02 Aug 2022 15:31:44: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 15:31:44: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 15:31:46: #2 number of paired peaks: 50 WARNING @ Tue, 02 Aug 2022 15:31:46: Too few paired peaks (50) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 15:31:46: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX13227890/SRX13227890.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13227890/SRX13227890.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13227890/SRX13227890.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13227890/SRX13227890.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。