Job ID = 16439325 SRX = SRX13227889 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 36353930 spots for SRR17038002/SRR17038002.sra Written 36353930 spots for SRR17038002/SRR17038002.sra fastq に変換しました。 bowtie でマッピング中... Your job 16439493 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:01 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:20:14 36353930 reads; of these: 36353930 (100.00%) were unpaired; of these: 1386282 (3.81%) aligned 0 times 26244823 (72.19%) aligned exactly 1 time 8722825 (23.99%) aligned >1 times 96.19% overall alignment rate Time searching: 00:20:15 Overall time: 00:20:15 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 4386696 / 34967648 = 0.1255 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 15:31:09: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX13227889/SRX13227889.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX13227889/SRX13227889.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX13227889/SRX13227889.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX13227889/SRX13227889.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 15:31:09: #1 read tag files... INFO @ Tue, 02 Aug 2022 15:31:09: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 15:31:19: 1000000 INFO @ Tue, 02 Aug 2022 15:31:29: 2000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 15:31:39: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX13227889/SRX13227889.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX13227889/SRX13227889.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX13227889/SRX13227889.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX13227889/SRX13227889.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 15:31:39: #1 read tag files... INFO @ Tue, 02 Aug 2022 15:31:39: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 15:31:39: 3000000 INFO @ Tue, 02 Aug 2022 15:31:50: 4000000 INFO @ Tue, 02 Aug 2022 15:31:52: 1000000 INFO @ Tue, 02 Aug 2022 15:32:01: 5000000 INFO @ Tue, 02 Aug 2022 15:32:05: 2000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 15:32:09: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX13227889/SRX13227889.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX13227889/SRX13227889.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX13227889/SRX13227889.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX13227889/SRX13227889.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 15:32:09: #1 read tag files... INFO @ Tue, 02 Aug 2022 15:32:09: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 15:32:12: 6000000 INFO @ Tue, 02 Aug 2022 15:32:18: 3000000 INFO @ Tue, 02 Aug 2022 15:32:21: 1000000 INFO @ Tue, 02 Aug 2022 15:32:22: 7000000 INFO @ Tue, 02 Aug 2022 15:32:31: 4000000 INFO @ Tue, 02 Aug 2022 15:32:31: 2000000 INFO @ Tue, 02 Aug 2022 15:32:32: 8000000 INFO @ Tue, 02 Aug 2022 15:32:41: 3000000 INFO @ Tue, 02 Aug 2022 15:32:41: 9000000 INFO @ Tue, 02 Aug 2022 15:32:43: 5000000 INFO @ Tue, 02 Aug 2022 15:32:51: 10000000 INFO @ Tue, 02 Aug 2022 15:32:52: 4000000 INFO @ Tue, 02 Aug 2022 15:32:56: 6000000 INFO @ Tue, 02 Aug 2022 15:33:00: 11000000 INFO @ Tue, 02 Aug 2022 15:33:03: 5000000 INFO @ Tue, 02 Aug 2022 15:33:08: 7000000 INFO @ Tue, 02 Aug 2022 15:33:09: 12000000 INFO @ Tue, 02 Aug 2022 15:33:13: 6000000 INFO @ Tue, 02 Aug 2022 15:33:19: 13000000 INFO @ Tue, 02 Aug 2022 15:33:21: 8000000 INFO @ Tue, 02 Aug 2022 15:33:24: 7000000 INFO @ Tue, 02 Aug 2022 15:33:29: 14000000 INFO @ Tue, 02 Aug 2022 15:33:33: 9000000 INFO @ Tue, 02 Aug 2022 15:33:34: 8000000 INFO @ Tue, 02 Aug 2022 15:33:39: 15000000 INFO @ Tue, 02 Aug 2022 15:33:45: 9000000 INFO @ Tue, 02 Aug 2022 15:33:46: 10000000 INFO @ Tue, 02 Aug 2022 15:33:49: 16000000 INFO @ Tue, 02 Aug 2022 15:33:55: 10000000 INFO @ Tue, 02 Aug 2022 15:33:58: 17000000 INFO @ Tue, 02 Aug 2022 15:33:59: 11000000 INFO @ Tue, 02 Aug 2022 15:34:05: 11000000 INFO @ Tue, 02 Aug 2022 15:34:08: 18000000 INFO @ Tue, 02 Aug 2022 15:34:11: 12000000 INFO @ Tue, 02 Aug 2022 15:34:16: 12000000 INFO @ Tue, 02 Aug 2022 15:34:17: 19000000 INFO @ Tue, 02 Aug 2022 15:34:24: 13000000 INFO @ Tue, 02 Aug 2022 15:34:26: 13000000 INFO @ Tue, 02 Aug 2022 15:34:28: 20000000 INFO @ Tue, 02 Aug 2022 15:34:36: 14000000 INFO @ Tue, 02 Aug 2022 15:34:37: 14000000 INFO @ Tue, 02 Aug 2022 15:34:38: 21000000 INFO @ Tue, 02 Aug 2022 15:34:47: 15000000 INFO @ Tue, 02 Aug 2022 15:34:48: 22000000 INFO @ Tue, 02 Aug 2022 15:34:49: 15000000 INFO @ Tue, 02 Aug 2022 15:34:57: 16000000 INFO @ Tue, 02 Aug 2022 15:34:59: 23000000 INFO @ Tue, 02 Aug 2022 15:35:02: 16000000 INFO @ Tue, 02 Aug 2022 15:35:08: 17000000 INFO @ Tue, 02 Aug 2022 15:35:09: 24000000 INFO @ Tue, 02 Aug 2022 15:35:14: 17000000 INFO @ Tue, 02 Aug 2022 15:35:19: 18000000 INFO @ Tue, 02 Aug 2022 15:35:20: 25000000 INFO @ Tue, 02 Aug 2022 15:35:27: 18000000 INFO @ Tue, 02 Aug 2022 15:35:30: 19000000 INFO @ Tue, 02 Aug 2022 15:35:31: 26000000 INFO @ Tue, 02 Aug 2022 15:35:39: 19000000 INFO @ Tue, 02 Aug 2022 15:35:41: 20000000 INFO @ Tue, 02 Aug 2022 15:35:42: 27000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 02 Aug 2022 15:35:51: 20000000 INFO @ Tue, 02 Aug 2022 15:35:52: 28000000 INFO @ Tue, 02 Aug 2022 15:35:53: 21000000 INFO @ Tue, 02 Aug 2022 15:36:02: 21000000 INFO @ Tue, 02 Aug 2022 15:36:03: 29000000 INFO @ Tue, 02 Aug 2022 15:36:04: 22000000 INFO @ Tue, 02 Aug 2022 15:36:13: 30000000 INFO @ Tue, 02 Aug 2022 15:36:14: 22000000 INFO @ Tue, 02 Aug 2022 15:36:16: 23000000 INFO @ Tue, 02 Aug 2022 15:36:20: #1 tag size is determined as 74 bps INFO @ Tue, 02 Aug 2022 15:36:20: #1 tag size = 74 INFO @ Tue, 02 Aug 2022 15:36:20: #1 total tags in treatment: 30580952 INFO @ Tue, 02 Aug 2022 15:36:20: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 15:36:20: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 15:36:20: #1 tags after filtering in treatment: 30580952 INFO @ Tue, 02 Aug 2022 15:36:20: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 15:36:20: #1 finished! INFO @ Tue, 02 Aug 2022 15:36:20: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 15:36:20: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 15:36:23: #2 number of paired peaks: 5 WARNING @ Tue, 02 Aug 2022 15:36:23: Too few paired peaks (5) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 15:36:23: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX13227889/SRX13227889.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13227889/SRX13227889.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13227889/SRX13227889.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13227889/SRX13227889.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 15:36:26: 23000000 INFO @ Tue, 02 Aug 2022 15:36:28: 24000000 INFO @ Tue, 02 Aug 2022 15:36:38: 24000000 INFO @ Tue, 02 Aug 2022 15:36:39: 25000000 INFO @ Tue, 02 Aug 2022 15:36:50: 25000000 INFO @ Tue, 02 Aug 2022 15:36:51: 26000000 INFO @ Tue, 02 Aug 2022 15:37:02: 26000000 INFO @ Tue, 02 Aug 2022 15:37:02: 27000000 INFO @ Tue, 02 Aug 2022 15:37:13: 28000000 INFO @ Tue, 02 Aug 2022 15:37:13: 27000000 INFO @ Tue, 02 Aug 2022 15:37:24: 29000000 INFO @ Tue, 02 Aug 2022 15:37:25: 28000000 BigWig に変換しました。 INFO @ Tue, 02 Aug 2022 15:37:35: 30000000 INFO @ Tue, 02 Aug 2022 15:37:36: 29000000 INFO @ Tue, 02 Aug 2022 15:37:41: #1 tag size is determined as 74 bps INFO @ Tue, 02 Aug 2022 15:37:41: #1 tag size = 74 INFO @ Tue, 02 Aug 2022 15:37:41: #1 total tags in treatment: 30580952 INFO @ Tue, 02 Aug 2022 15:37:41: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 15:37:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 15:37:42: #1 tags after filtering in treatment: 30580952 INFO @ Tue, 02 Aug 2022 15:37:42: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 15:37:42: #1 finished! INFO @ Tue, 02 Aug 2022 15:37:42: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 15:37:42: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 15:37:44: #2 number of paired peaks: 5 WARNING @ Tue, 02 Aug 2022 15:37:44: Too few paired peaks (5) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 15:37:44: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX13227889/SRX13227889.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13227889/SRX13227889.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13227889/SRX13227889.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13227889/SRX13227889.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 15:37:48: 30000000 INFO @ Tue, 02 Aug 2022 15:37:54: #1 tag size is determined as 74 bps INFO @ Tue, 02 Aug 2022 15:37:54: #1 tag size = 74 INFO @ Tue, 02 Aug 2022 15:37:54: #1 total tags in treatment: 30580952 INFO @ Tue, 02 Aug 2022 15:37:54: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 15:37:54: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 15:37:55: #1 tags after filtering in treatment: 30580952 INFO @ Tue, 02 Aug 2022 15:37:55: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 15:37:55: #1 finished! INFO @ Tue, 02 Aug 2022 15:37:55: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 15:37:55: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 15:37:58: #2 number of paired peaks: 5 WARNING @ Tue, 02 Aug 2022 15:37:58: Too few paired peaks (5) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 15:37:58: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX13227889/SRX13227889.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13227889/SRX13227889.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13227889/SRX13227889.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13227889/SRX13227889.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling