Job ID = 9158440 sra ファイルのダウンロード中... Completed: 783162K bytes transferred in 9 seconds (705577K bits/sec), in 1 file, 2 directories. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 22184316 spots for /home/okishinya/chipatlas/results/dm3/SRX1300698/SRR2548374.sra Written 22184316 spots total rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:08:10 22184316 reads; of these: 22184316 (100.00%) were unpaired; of these: 2610176 (11.77%) aligned 0 times 13892626 (62.62%) aligned exactly 1 time 5681514 (25.61%) aligned >1 times 88.23% overall alignment rate Time searching: 00:08:10 Overall time: 00:08:10 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 2076647 / 19574140 = 0.1061 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Tue, 27 Jun 2017 16:59:17: # Command line: callpeak -t SRX1300698.bam -f BAM -g dm -n SRX1300698.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX1300698.10 # format = BAM # ChIP-seq file = ['SRX1300698.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 16:59:17: #1 read tag files... INFO @ Tue, 27 Jun 2017 16:59:17: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 16:59:17: # Command line: callpeak -t SRX1300698.bam -f BAM -g dm -n SRX1300698.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX1300698.05 # format = BAM # ChIP-seq file = ['SRX1300698.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 16:59:17: #1 read tag files... INFO @ Tue, 27 Jun 2017 16:59:17: # Command line: callpeak -t SRX1300698.bam -f BAM -g dm -n SRX1300698.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX1300698.20 # format = BAM # ChIP-seq file = ['SRX1300698.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 16:59:17: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 16:59:17: #1 read tag files... INFO @ Tue, 27 Jun 2017 16:59:17: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 16:59:23: 1000000 INFO @ Tue, 27 Jun 2017 16:59:23: 1000000 INFO @ Tue, 27 Jun 2017 16:59:23: 1000000 INFO @ Tue, 27 Jun 2017 16:59:29: 2000000 INFO @ Tue, 27 Jun 2017 16:59:29: 2000000 INFO @ Tue, 27 Jun 2017 16:59:30: 2000000 INFO @ Tue, 27 Jun 2017 16:59:35: 3000000 INFO @ Tue, 27 Jun 2017 16:59:36: 3000000 INFO @ Tue, 27 Jun 2017 16:59:36: 3000000 INFO @ Tue, 27 Jun 2017 16:59:41: 4000000 INFO @ Tue, 27 Jun 2017 16:59:42: 4000000 INFO @ Tue, 27 Jun 2017 16:59:43: 4000000 INFO @ Tue, 27 Jun 2017 16:59:47: 5000000 INFO @ Tue, 27 Jun 2017 16:59:48: 5000000 INFO @ Tue, 27 Jun 2017 16:59:49: 5000000 INFO @ Tue, 27 Jun 2017 16:59:53: 6000000 INFO @ Tue, 27 Jun 2017 16:59:54: 6000000 INFO @ Tue, 27 Jun 2017 16:59:56: 6000000 INFO @ Tue, 27 Jun 2017 16:59:59: 7000000 INFO @ Tue, 27 Jun 2017 17:00:01: 7000000 INFO @ Tue, 27 Jun 2017 17:00:02: 7000000 INFO @ Tue, 27 Jun 2017 17:00:05: 8000000 INFO @ Tue, 27 Jun 2017 17:00:07: 8000000 INFO @ Tue, 27 Jun 2017 17:00:09: 8000000 INFO @ Tue, 27 Jun 2017 17:00:11: 9000000 INFO @ Tue, 27 Jun 2017 17:00:13: 9000000 INFO @ Tue, 27 Jun 2017 17:00:15: 9000000 INFO @ Tue, 27 Jun 2017 17:00:17: 10000000 INFO @ Tue, 27 Jun 2017 17:00:20: 10000000 INFO @ Tue, 27 Jun 2017 17:00:22: 10000000 INFO @ Tue, 27 Jun 2017 17:00:24: 11000000 INFO @ Tue, 27 Jun 2017 17:00:26: 11000000 INFO @ Tue, 27 Jun 2017 17:00:28: 11000000 INFO @ Tue, 27 Jun 2017 17:00:30: 12000000 INFO @ Tue, 27 Jun 2017 17:00:32: 12000000 INFO @ Tue, 27 Jun 2017 17:00:35: 12000000 INFO @ Tue, 27 Jun 2017 17:00:36: 13000000 INFO @ Tue, 27 Jun 2017 17:00:38: 13000000 INFO @ Tue, 27 Jun 2017 17:00:41: 13000000 INFO @ Tue, 27 Jun 2017 17:00:42: 14000000 INFO @ Tue, 27 Jun 2017 17:00:45: 14000000 INFO @ Tue, 27 Jun 2017 17:00:48: 14000000 INFO @ Tue, 27 Jun 2017 17:00:48: 15000000 INFO @ Tue, 27 Jun 2017 17:00:51: 15000000 INFO @ Tue, 27 Jun 2017 17:00:54: 16000000 INFO @ Tue, 27 Jun 2017 17:00:54: 15000000 INFO @ Tue, 27 Jun 2017 17:00:57: 16000000 INFO @ Tue, 27 Jun 2017 17:01:00: 17000000 INFO @ Tue, 27 Jun 2017 17:01:01: 16000000 INFO @ Tue, 27 Jun 2017 17:01:03: #1 tag size is determined as 50 bps INFO @ Tue, 27 Jun 2017 17:01:03: #1 tag size = 50 INFO @ Tue, 27 Jun 2017 17:01:03: #1 total tags in treatment: 17497493 INFO @ Tue, 27 Jun 2017 17:01:03: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 17:01:03: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 17:01:03: 17000000 INFO @ Tue, 27 Jun 2017 17:01:03: #1 tags after filtering in treatment: 17497493 INFO @ Tue, 27 Jun 2017 17:01:03: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 17:01:03: #1 finished! INFO @ Tue, 27 Jun 2017 17:01:03: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 17:01:03: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 17:01:05: #2 number of paired peaks: 75 WARNING @ Tue, 27 Jun 2017 17:01:05: Too few paired peaks (75) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 17:01:05: Process for pairing-model is terminated! cat: SRX1300698.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1300698.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1300698.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1300698.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Tue, 27 Jun 2017 17:01:07: #1 tag size is determined as 50 bps INFO @ Tue, 27 Jun 2017 17:01:07: #1 tag size = 50 INFO @ Tue, 27 Jun 2017 17:01:07: #1 total tags in treatment: 17497493 INFO @ Tue, 27 Jun 2017 17:01:07: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 17:01:07: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 17:01:07: #1 tags after filtering in treatment: 17497493 INFO @ Tue, 27 Jun 2017 17:01:07: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 17:01:07: #1 finished! INFO @ Tue, 27 Jun 2017 17:01:07: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 17:01:07: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 17:01:07: 17000000 INFO @ Tue, 27 Jun 2017 17:01:08: #2 number of paired peaks: 75 WARNING @ Tue, 27 Jun 2017 17:01:08: Too few paired peaks (75) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 17:01:08: Process for pairing-model is terminated! cat: SRX1300698.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1300698.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1300698.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1300698.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Tue, 27 Jun 2017 17:01:10: #1 tag size is determined as 50 bps INFO @ Tue, 27 Jun 2017 17:01:10: #1 tag size = 50 INFO @ Tue, 27 Jun 2017 17:01:10: #1 total tags in treatment: 17497493 INFO @ Tue, 27 Jun 2017 17:01:10: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 17:01:10: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 17:01:11: #1 tags after filtering in treatment: 17497493 INFO @ Tue, 27 Jun 2017 17:01:11: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 17:01:11: #1 finished! INFO @ Tue, 27 Jun 2017 17:01:11: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 17:01:11: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 17:01:12: #2 number of paired peaks: 75 WARNING @ Tue, 27 Jun 2017 17:01:12: Too few paired peaks (75) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 17:01:12: Process for pairing-model is terminated! cat: SRX1300698.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1300698.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1300698.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1300698.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。