Job ID = 9158439 sra ファイルのダウンロード中... Completed: 732111K bytes transferred in 10 seconds (575346K bits/sec), in 1 file, 2 directories. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 20685811 spots for /home/okishinya/chipatlas/results/dm3/SRX1300697/SRR2548373.sra Written 20685811 spots total rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:07:59 20685811 reads; of these: 20685811 (100.00%) were unpaired; of these: 1581084 (7.64%) aligned 0 times 13483354 (65.18%) aligned exactly 1 time 5621373 (27.18%) aligned >1 times 92.36% overall alignment rate Time searching: 00:07:59 Overall time: 00:07:59 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 2146667 / 19104727 = 0.1124 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Tue, 27 Jun 2017 16:58:58: # Command line: callpeak -t SRX1300697.bam -f BAM -g dm -n SRX1300697.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX1300697.05 # format = BAM # ChIP-seq file = ['SRX1300697.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 16:58:58: #1 read tag files... INFO @ Tue, 27 Jun 2017 16:58:58: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 16:58:58: # Command line: callpeak -t SRX1300697.bam -f BAM -g dm -n SRX1300697.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX1300697.10 # format = BAM # ChIP-seq file = ['SRX1300697.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 16:58:58: #1 read tag files... INFO @ Tue, 27 Jun 2017 16:58:58: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 16:58:58: # Command line: callpeak -t SRX1300697.bam -f BAM -g dm -n SRX1300697.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX1300697.20 # format = BAM # ChIP-seq file = ['SRX1300697.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 16:58:58: #1 read tag files... INFO @ Tue, 27 Jun 2017 16:58:58: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 16:59:06: 1000000 INFO @ Tue, 27 Jun 2017 16:59:06: 1000000 INFO @ Tue, 27 Jun 2017 16:59:07: 1000000 INFO @ Tue, 27 Jun 2017 16:59:14: 2000000 INFO @ Tue, 27 Jun 2017 16:59:15: 2000000 INFO @ Tue, 27 Jun 2017 16:59:15: 2000000 INFO @ Tue, 27 Jun 2017 16:59:23: 3000000 INFO @ Tue, 27 Jun 2017 16:59:24: 3000000 INFO @ Tue, 27 Jun 2017 16:59:24: 3000000 INFO @ Tue, 27 Jun 2017 16:59:31: 4000000 INFO @ Tue, 27 Jun 2017 16:59:33: 4000000 INFO @ Tue, 27 Jun 2017 16:59:33: 4000000 INFO @ Tue, 27 Jun 2017 16:59:40: 5000000 INFO @ Tue, 27 Jun 2017 16:59:42: 5000000 INFO @ Tue, 27 Jun 2017 16:59:42: 5000000 INFO @ Tue, 27 Jun 2017 16:59:48: 6000000 INFO @ Tue, 27 Jun 2017 16:59:51: 6000000 INFO @ Tue, 27 Jun 2017 16:59:51: 6000000 INFO @ Tue, 27 Jun 2017 16:59:57: 7000000 INFO @ Tue, 27 Jun 2017 16:59:59: 7000000 INFO @ Tue, 27 Jun 2017 16:59:59: 7000000 INFO @ Tue, 27 Jun 2017 17:00:05: 8000000 INFO @ Tue, 27 Jun 2017 17:00:08: 8000000 INFO @ Tue, 27 Jun 2017 17:00:08: 8000000 INFO @ Tue, 27 Jun 2017 17:00:14: 9000000 INFO @ Tue, 27 Jun 2017 17:00:16: 9000000 INFO @ Tue, 27 Jun 2017 17:00:16: 9000000 INFO @ Tue, 27 Jun 2017 17:00:22: 10000000 INFO @ Tue, 27 Jun 2017 17:00:24: 10000000 INFO @ Tue, 27 Jun 2017 17:00:24: 10000000 INFO @ Tue, 27 Jun 2017 17:00:30: 11000000 INFO @ Tue, 27 Jun 2017 17:00:31: 11000000 INFO @ Tue, 27 Jun 2017 17:00:31: 11000000 INFO @ Tue, 27 Jun 2017 17:00:37: 12000000 INFO @ Tue, 27 Jun 2017 17:00:39: 12000000 INFO @ Tue, 27 Jun 2017 17:00:39: 12000000 INFO @ Tue, 27 Jun 2017 17:00:44: 13000000 INFO @ Tue, 27 Jun 2017 17:00:47: 13000000 INFO @ Tue, 27 Jun 2017 17:00:47: 13000000 INFO @ Tue, 27 Jun 2017 17:00:51: 14000000 INFO @ Tue, 27 Jun 2017 17:00:54: 14000000 INFO @ Tue, 27 Jun 2017 17:00:54: 14000000 INFO @ Tue, 27 Jun 2017 17:00:58: 15000000 INFO @ Tue, 27 Jun 2017 17:01:02: 15000000 INFO @ Tue, 27 Jun 2017 17:01:02: 15000000 INFO @ Tue, 27 Jun 2017 17:01:05: 16000000 INFO @ Tue, 27 Jun 2017 17:01:09: 16000000 INFO @ Tue, 27 Jun 2017 17:01:09: 16000000 INFO @ Tue, 27 Jun 2017 17:01:12: #1 tag size is determined as 50 bps INFO @ Tue, 27 Jun 2017 17:01:12: #1 tag size = 50 INFO @ Tue, 27 Jun 2017 17:01:12: #1 total tags in treatment: 16958060 INFO @ Tue, 27 Jun 2017 17:01:12: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 17:01:12: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 17:01:12: #1 tags after filtering in treatment: 16958060 INFO @ Tue, 27 Jun 2017 17:01:12: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 17:01:12: #1 finished! INFO @ Tue, 27 Jun 2017 17:01:12: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 17:01:12: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 17:01:13: #2 number of paired peaks: 68 WARNING @ Tue, 27 Jun 2017 17:01:13: Too few paired peaks (68) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 17:01:13: Process for pairing-model is terminated! cat: SRX1300697.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1300697.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1300697.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1300697.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Tue, 27 Jun 2017 17:01:17: #1 tag size is determined as 50 bps INFO @ Tue, 27 Jun 2017 17:01:17: #1 tag size = 50 INFO @ Tue, 27 Jun 2017 17:01:17: #1 total tags in treatment: 16958060 INFO @ Tue, 27 Jun 2017 17:01:17: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 17:01:17: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 17:01:17: #1 tag size is determined as 50 bps INFO @ Tue, 27 Jun 2017 17:01:17: #1 tag size = 50 INFO @ Tue, 27 Jun 2017 17:01:17: #1 total tags in treatment: 16958060 INFO @ Tue, 27 Jun 2017 17:01:17: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 17:01:17: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 17:01:17: #1 tags after filtering in treatment: 16958060 INFO @ Tue, 27 Jun 2017 17:01:17: #1 tags after filtering in treatment: 16958060 INFO @ Tue, 27 Jun 2017 17:01:17: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 17:01:17: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 17:01:17: #1 finished! INFO @ Tue, 27 Jun 2017 17:01:17: #1 finished! INFO @ Tue, 27 Jun 2017 17:01:17: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 17:01:17: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 17:01:17: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 17:01:17: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 17:01:18: #2 number of paired peaks: 68 WARNING @ Tue, 27 Jun 2017 17:01:18: Too few paired peaks (68) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 17:01:18: Process for pairing-model is terminated! INFO @ Tue, 27 Jun 2017 17:01:18: #2 number of paired peaks: 68 WARNING @ Tue, 27 Jun 2017 17:01:18: Too few paired peaks (68) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 17:01:18: Process for pairing-model is terminated! cat: SRX1300697.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません cat: SRX1300697.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) pass1 - making usageList (0 chroms): 1 millis rm: cannot remove `SRX1300697.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1300697.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1300697.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません needLargeMem: trying to allocate 0 bytes (limit: 17179869184) CompletedMACS2peakCalling rm: cannot remove `SRX1300697.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1300697.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1300697.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。