Job ID = 9158435 sra ファイルのダウンロード中... Completed: 745442K bytes transferred in 9 seconds (659217K bits/sec), in 1 file, 2 directories. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 21046604 spots for /home/okishinya/chipatlas/results/dm3/SRX1300694/SRR2548370.sra Written 21046604 spots total rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:09:22 21046604 reads; of these: 21046604 (100.00%) were unpaired; of these: 835214 (3.97%) aligned 0 times 14074447 (66.87%) aligned exactly 1 time 6136943 (29.16%) aligned >1 times 96.03% overall alignment rate Time searching: 00:09:22 Overall time: 00:09:22 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 1896954 / 20211390 = 0.0939 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Tue, 27 Jun 2017 17:00:09: # Command line: callpeak -t SRX1300694.bam -f BAM -g dm -n SRX1300694.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX1300694.20 # format = BAM # ChIP-seq file = ['SRX1300694.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 17:00:09: #1 read tag files... INFO @ Tue, 27 Jun 2017 17:00:09: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 17:00:09: # Command line: callpeak -t SRX1300694.bam -f BAM -g dm -n SRX1300694.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX1300694.05 # format = BAM # ChIP-seq file = ['SRX1300694.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 17:00:09: #1 read tag files... INFO @ Tue, 27 Jun 2017 17:00:09: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 17:00:09: # Command line: callpeak -t SRX1300694.bam -f BAM -g dm -n SRX1300694.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX1300694.10 # format = BAM # ChIP-seq file = ['SRX1300694.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 17:00:09: #1 read tag files... INFO @ Tue, 27 Jun 2017 17:00:09: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 17:00:17: 1000000 INFO @ Tue, 27 Jun 2017 17:00:17: 1000000 INFO @ Tue, 27 Jun 2017 17:00:18: 1000000 INFO @ Tue, 27 Jun 2017 17:00:26: 2000000 INFO @ Tue, 27 Jun 2017 17:00:26: 2000000 INFO @ Tue, 27 Jun 2017 17:00:27: 2000000 INFO @ Tue, 27 Jun 2017 17:00:34: 3000000 INFO @ Tue, 27 Jun 2017 17:00:34: 3000000 INFO @ Tue, 27 Jun 2017 17:00:37: 3000000 INFO @ Tue, 27 Jun 2017 17:00:42: 4000000 INFO @ Tue, 27 Jun 2017 17:00:42: 4000000 INFO @ Tue, 27 Jun 2017 17:00:46: 4000000 INFO @ Tue, 27 Jun 2017 17:00:51: 5000000 INFO @ Tue, 27 Jun 2017 17:00:51: 5000000 INFO @ Tue, 27 Jun 2017 17:00:55: 5000000 INFO @ Tue, 27 Jun 2017 17:00:59: 6000000 INFO @ Tue, 27 Jun 2017 17:00:59: 6000000 INFO @ Tue, 27 Jun 2017 17:01:04: 6000000 INFO @ Tue, 27 Jun 2017 17:01:07: 7000000 INFO @ Tue, 27 Jun 2017 17:01:07: 7000000 INFO @ Tue, 27 Jun 2017 17:01:14: 7000000 INFO @ Tue, 27 Jun 2017 17:01:16: 8000000 INFO @ Tue, 27 Jun 2017 17:01:16: 8000000 INFO @ Tue, 27 Jun 2017 17:01:23: 8000000 INFO @ Tue, 27 Jun 2017 17:01:25: 9000000 INFO @ Tue, 27 Jun 2017 17:01:25: 9000000 INFO @ Tue, 27 Jun 2017 17:01:32: 9000000 INFO @ Tue, 27 Jun 2017 17:01:33: 10000000 INFO @ Tue, 27 Jun 2017 17:01:33: 10000000 INFO @ Tue, 27 Jun 2017 17:01:41: 11000000 INFO @ Tue, 27 Jun 2017 17:01:41: 11000000 INFO @ Tue, 27 Jun 2017 17:01:42: 10000000 INFO @ Tue, 27 Jun 2017 17:01:50: 12000000 INFO @ Tue, 27 Jun 2017 17:01:50: 12000000 INFO @ Tue, 27 Jun 2017 17:01:51: 11000000 INFO @ Tue, 27 Jun 2017 17:01:57: 13000000 INFO @ Tue, 27 Jun 2017 17:01:58: 13000000 INFO @ Tue, 27 Jun 2017 17:02:00: 12000000 INFO @ Tue, 27 Jun 2017 17:02:03: 14000000 INFO @ Tue, 27 Jun 2017 17:02:06: 14000000 INFO @ Tue, 27 Jun 2017 17:02:09: 13000000 INFO @ Tue, 27 Jun 2017 17:02:10: 15000000 INFO @ Tue, 27 Jun 2017 17:02:13: 15000000 INFO @ Tue, 27 Jun 2017 17:02:16: 14000000 INFO @ Tue, 27 Jun 2017 17:02:17: 16000000 INFO @ Tue, 27 Jun 2017 17:02:20: 16000000 INFO @ Tue, 27 Jun 2017 17:02:23: 17000000 INFO @ Tue, 27 Jun 2017 17:02:24: 15000000 INFO @ Tue, 27 Jun 2017 17:02:28: 17000000 INFO @ Tue, 27 Jun 2017 17:02:30: 18000000 INFO @ Tue, 27 Jun 2017 17:02:32: 16000000 INFO @ Tue, 27 Jun 2017 17:02:32: #1 tag size is determined as 50 bps INFO @ Tue, 27 Jun 2017 17:02:32: #1 tag size = 50 INFO @ Tue, 27 Jun 2017 17:02:32: #1 total tags in treatment: 18314436 INFO @ Tue, 27 Jun 2017 17:02:32: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 17:02:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 17:02:33: #1 tags after filtering in treatment: 18314436 INFO @ Tue, 27 Jun 2017 17:02:33: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 17:02:33: #1 finished! INFO @ Tue, 27 Jun 2017 17:02:33: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 17:02:33: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 17:02:34: #2 number of paired peaks: 82 WARNING @ Tue, 27 Jun 2017 17:02:34: Too few paired peaks (82) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 17:02:34: Process for pairing-model is terminated! cat: SRX1300694.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1300694.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1300694.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1300694.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Tue, 27 Jun 2017 17:02:35: 18000000 INFO @ Tue, 27 Jun 2017 17:02:37: #1 tag size is determined as 50 bps INFO @ Tue, 27 Jun 2017 17:02:37: #1 tag size = 50 INFO @ Tue, 27 Jun 2017 17:02:37: #1 total tags in treatment: 18314436 INFO @ Tue, 27 Jun 2017 17:02:37: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 17:02:37: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 17:02:38: #1 tags after filtering in treatment: 18314436 INFO @ Tue, 27 Jun 2017 17:02:38: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 17:02:38: #1 finished! INFO @ Tue, 27 Jun 2017 17:02:38: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 17:02:38: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 17:02:39: #2 number of paired peaks: 82 WARNING @ Tue, 27 Jun 2017 17:02:39: Too few paired peaks (82) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 17:02:39: Process for pairing-model is terminated! cat: SRX1300694.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1300694.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1300694.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1300694.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Tue, 27 Jun 2017 17:02:39: 17000000 INFO @ Tue, 27 Jun 2017 17:02:46: 18000000 INFO @ Tue, 27 Jun 2017 17:02:48: #1 tag size is determined as 50 bps INFO @ Tue, 27 Jun 2017 17:02:48: #1 tag size = 50 INFO @ Tue, 27 Jun 2017 17:02:48: #1 total tags in treatment: 18314436 INFO @ Tue, 27 Jun 2017 17:02:48: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 17:02:48: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 17:02:48: #1 tags after filtering in treatment: 18314436 INFO @ Tue, 27 Jun 2017 17:02:48: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 17:02:48: #1 finished! INFO @ Tue, 27 Jun 2017 17:02:48: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 17:02:48: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 17:02:49: #2 number of paired peaks: 82 WARNING @ Tue, 27 Jun 2017 17:02:49: Too few paired peaks (82) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 17:02:49: Process for pairing-model is terminated! cat: SRX1300694.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1300694.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1300694.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1300694.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。