Job ID = 9158431 sra ファイルのダウンロード中... Completed: 814667K bytes transferred in 9 seconds (682607K bits/sec), in 1 file, 2 directories. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 23006347 spots for /home/okishinya/chipatlas/results/dm3/SRX1300692/SRR2548368.sra Written 23006347 spots total rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:09:34 23006347 reads; of these: 23006347 (100.00%) were unpaired; of these: 932161 (4.05%) aligned 0 times 15289899 (66.46%) aligned exactly 1 time 6784287 (29.49%) aligned >1 times 95.95% overall alignment rate Time searching: 00:09:34 Overall time: 00:09:34 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 2228537 / 22074186 = 0.1010 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Tue, 27 Jun 2017 17:00:32: # Command line: callpeak -t SRX1300692.bam -f BAM -g dm -n SRX1300692.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX1300692.10 # format = BAM # ChIP-seq file = ['SRX1300692.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 17:00:32: #1 read tag files... INFO @ Tue, 27 Jun 2017 17:00:32: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 17:00:32: # Command line: callpeak -t SRX1300692.bam -f BAM -g dm -n SRX1300692.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX1300692.20 # format = BAM # ChIP-seq file = ['SRX1300692.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 17:00:32: #1 read tag files... INFO @ Tue, 27 Jun 2017 17:00:32: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 17:00:32: # Command line: callpeak -t SRX1300692.bam -f BAM -g dm -n SRX1300692.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX1300692.05 # format = BAM # ChIP-seq file = ['SRX1300692.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 17:00:32: #1 read tag files... INFO @ Tue, 27 Jun 2017 17:00:32: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 17:00:39: 1000000 INFO @ Tue, 27 Jun 2017 17:00:39: 1000000 INFO @ Tue, 27 Jun 2017 17:00:39: 1000000 INFO @ Tue, 27 Jun 2017 17:00:46: 2000000 INFO @ Tue, 27 Jun 2017 17:00:46: 2000000 INFO @ Tue, 27 Jun 2017 17:00:46: 2000000 INFO @ Tue, 27 Jun 2017 17:00:53: 3000000 INFO @ Tue, 27 Jun 2017 17:00:54: 3000000 INFO @ Tue, 27 Jun 2017 17:00:54: 3000000 INFO @ Tue, 27 Jun 2017 17:01:01: 4000000 INFO @ Tue, 27 Jun 2017 17:01:01: 4000000 INFO @ Tue, 27 Jun 2017 17:01:01: 4000000 INFO @ Tue, 27 Jun 2017 17:01:08: 5000000 INFO @ Tue, 27 Jun 2017 17:01:08: 5000000 INFO @ Tue, 27 Jun 2017 17:01:08: 5000000 INFO @ Tue, 27 Jun 2017 17:01:15: 6000000 INFO @ Tue, 27 Jun 2017 17:01:16: 6000000 INFO @ Tue, 27 Jun 2017 17:01:16: 6000000 INFO @ Tue, 27 Jun 2017 17:01:23: 7000000 INFO @ Tue, 27 Jun 2017 17:01:23: 7000000 INFO @ Tue, 27 Jun 2017 17:01:23: 7000000 INFO @ Tue, 27 Jun 2017 17:01:30: 8000000 INFO @ Tue, 27 Jun 2017 17:01:30: 8000000 INFO @ Tue, 27 Jun 2017 17:01:30: 8000000 INFO @ Tue, 27 Jun 2017 17:01:37: 9000000 INFO @ Tue, 27 Jun 2017 17:01:38: 9000000 INFO @ Tue, 27 Jun 2017 17:01:38: 9000000 INFO @ Tue, 27 Jun 2017 17:01:45: 10000000 INFO @ Tue, 27 Jun 2017 17:01:45: 10000000 INFO @ Tue, 27 Jun 2017 17:01:45: 10000000 INFO @ Tue, 27 Jun 2017 17:01:52: 11000000 INFO @ Tue, 27 Jun 2017 17:01:52: 11000000 INFO @ Tue, 27 Jun 2017 17:01:52: 11000000 INFO @ Tue, 27 Jun 2017 17:01:59: 12000000 INFO @ Tue, 27 Jun 2017 17:01:59: 12000000 INFO @ Tue, 27 Jun 2017 17:01:59: 12000000 INFO @ Tue, 27 Jun 2017 17:02:06: 13000000 INFO @ Tue, 27 Jun 2017 17:02:07: 13000000 INFO @ Tue, 27 Jun 2017 17:02:07: 13000000 INFO @ Tue, 27 Jun 2017 17:02:13: 14000000 INFO @ Tue, 27 Jun 2017 17:02:14: 14000000 INFO @ Tue, 27 Jun 2017 17:02:14: 14000000 INFO @ Tue, 27 Jun 2017 17:02:21: 15000000 INFO @ Tue, 27 Jun 2017 17:02:21: 15000000 INFO @ Tue, 27 Jun 2017 17:02:21: 15000000 INFO @ Tue, 27 Jun 2017 17:02:28: 16000000 INFO @ Tue, 27 Jun 2017 17:02:29: 16000000 INFO @ Tue, 27 Jun 2017 17:02:29: 16000000 INFO @ Tue, 27 Jun 2017 17:02:35: 17000000 INFO @ Tue, 27 Jun 2017 17:02:36: 17000000 INFO @ Tue, 27 Jun 2017 17:02:36: 17000000 INFO @ Tue, 27 Jun 2017 17:02:42: 18000000 INFO @ Tue, 27 Jun 2017 17:02:43: 18000000 INFO @ Tue, 27 Jun 2017 17:02:43: 18000000 INFO @ Tue, 27 Jun 2017 17:02:50: 19000000 INFO @ Tue, 27 Jun 2017 17:02:50: 19000000 INFO @ Tue, 27 Jun 2017 17:02:50: 19000000 INFO @ Tue, 27 Jun 2017 17:02:56: #1 tag size is determined as 50 bps INFO @ Tue, 27 Jun 2017 17:02:56: #1 tag size = 50 INFO @ Tue, 27 Jun 2017 17:02:56: #1 total tags in treatment: 19845649 INFO @ Tue, 27 Jun 2017 17:02:56: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 17:02:56: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 17:02:56: #1 tags after filtering in treatment: 19845649 INFO @ Tue, 27 Jun 2017 17:02:56: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 17:02:56: #1 finished! INFO @ Tue, 27 Jun 2017 17:02:56: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 17:02:56: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 17:02:57: #1 tag size is determined as 50 bps INFO @ Tue, 27 Jun 2017 17:02:57: #1 tag size = 50 INFO @ Tue, 27 Jun 2017 17:02:57: #1 total tags in treatment: 19845649 INFO @ Tue, 27 Jun 2017 17:02:57: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 17:02:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 17:02:57: #1 tag size is determined as 50 bps INFO @ Tue, 27 Jun 2017 17:02:57: #1 tag size = 50 INFO @ Tue, 27 Jun 2017 17:02:57: #1 total tags in treatment: 19845649 INFO @ Tue, 27 Jun 2017 17:02:57: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 17:02:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 17:02:57: #1 tags after filtering in treatment: 19845649 INFO @ Tue, 27 Jun 2017 17:02:57: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 17:02:57: #1 finished! INFO @ Tue, 27 Jun 2017 17:02:57: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 17:02:57: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 17:02:57: #1 tags after filtering in treatment: 19845649 INFO @ Tue, 27 Jun 2017 17:02:57: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 17:02:57: #1 finished! INFO @ Tue, 27 Jun 2017 17:02:57: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 17:02:57: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 17:02:58: #2 number of paired peaks: 85 WARNING @ Tue, 27 Jun 2017 17:02:58: Too few paired peaks (85) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 17:02:58: Process for pairing-model is terminated! cat: SRX1300692.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1300692.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1300692.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1300692.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Tue, 27 Jun 2017 17:02:58: #2 number of paired peaks: 85 WARNING @ Tue, 27 Jun 2017 17:02:58: Too few paired peaks (85) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 17:02:58: Process for pairing-model is terminated! cat: SRX1300692.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1300692.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1300692.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1300692.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Tue, 27 Jun 2017 17:02:58: #2 number of paired peaks: 85 WARNING @ Tue, 27 Jun 2017 17:02:58: Too few paired peaks (85) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 17:02:58: Process for pairing-model is terminated! cat: SRX1300692.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1300692.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1300692.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1300692.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。