Job ID = 9158430 sra ファイルのダウンロード中... Completed: 890230K bytes transferred in 10 seconds (722311K bits/sec), in 1 file, 2 directories. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 25155192 spots for /home/okishinya/chipatlas/results/dm3/SRX1300691/SRR2548367.sra Written 25155192 spots total rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:01 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:10:18 25155192 reads; of these: 25155192 (100.00%) were unpaired; of these: 995810 (3.96%) aligned 0 times 16706677 (66.41%) aligned exactly 1 time 7452705 (29.63%) aligned >1 times 96.04% overall alignment rate Time searching: 00:10:19 Overall time: 00:10:19 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 2580427 / 24159382 = 0.1068 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Tue, 27 Jun 2017 17:00:09: # Command line: callpeak -t SRX1300691.bam -f BAM -g dm -n SRX1300691.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX1300691.05 # format = BAM # ChIP-seq file = ['SRX1300691.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 17:00:09: #1 read tag files... INFO @ Tue, 27 Jun 2017 17:00:09: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 17:00:09: # Command line: callpeak -t SRX1300691.bam -f BAM -g dm -n SRX1300691.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX1300691.20 # format = BAM # ChIP-seq file = ['SRX1300691.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 17:00:09: #1 read tag files... INFO @ Tue, 27 Jun 2017 17:00:09: # Command line: callpeak -t SRX1300691.bam -f BAM -g dm -n SRX1300691.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX1300691.10 # format = BAM # ChIP-seq file = ['SRX1300691.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 17:00:09: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 17:00:09: #1 read tag files... INFO @ Tue, 27 Jun 2017 17:00:09: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 17:00:17: 1000000 INFO @ Tue, 27 Jun 2017 17:00:17: 1000000 INFO @ Tue, 27 Jun 2017 17:00:17: 1000000 INFO @ Tue, 27 Jun 2017 17:00:25: 2000000 INFO @ Tue, 27 Jun 2017 17:00:25: 2000000 INFO @ Tue, 27 Jun 2017 17:00:25: 2000000 INFO @ Tue, 27 Jun 2017 17:00:34: 3000000 INFO @ Tue, 27 Jun 2017 17:00:34: 3000000 INFO @ Tue, 27 Jun 2017 17:00:34: 3000000 INFO @ Tue, 27 Jun 2017 17:00:42: 4000000 INFO @ Tue, 27 Jun 2017 17:00:42: 4000000 INFO @ Tue, 27 Jun 2017 17:00:42: 4000000 INFO @ Tue, 27 Jun 2017 17:00:50: 5000000 INFO @ Tue, 27 Jun 2017 17:00:50: 5000000 INFO @ Tue, 27 Jun 2017 17:00:50: 5000000 INFO @ Tue, 27 Jun 2017 17:00:58: 6000000 INFO @ Tue, 27 Jun 2017 17:00:58: 6000000 INFO @ Tue, 27 Jun 2017 17:00:58: 6000000 INFO @ Tue, 27 Jun 2017 17:01:06: 7000000 INFO @ Tue, 27 Jun 2017 17:01:06: 7000000 INFO @ Tue, 27 Jun 2017 17:01:06: 7000000 INFO @ Tue, 27 Jun 2017 17:01:14: 8000000 INFO @ Tue, 27 Jun 2017 17:01:14: 8000000 INFO @ Tue, 27 Jun 2017 17:01:14: 8000000 INFO @ Tue, 27 Jun 2017 17:01:21: 9000000 INFO @ Tue, 27 Jun 2017 17:01:21: 9000000 INFO @ Tue, 27 Jun 2017 17:01:21: 9000000 INFO @ Tue, 27 Jun 2017 17:01:29: 10000000 INFO @ Tue, 27 Jun 2017 17:01:29: 10000000 INFO @ Tue, 27 Jun 2017 17:01:29: 10000000 INFO @ Tue, 27 Jun 2017 17:01:38: 11000000 INFO @ Tue, 27 Jun 2017 17:01:38: 11000000 INFO @ Tue, 27 Jun 2017 17:01:38: 11000000 INFO @ Tue, 27 Jun 2017 17:01:46: 12000000 INFO @ Tue, 27 Jun 2017 17:01:46: 12000000 INFO @ Tue, 27 Jun 2017 17:01:46: 12000000 INFO @ Tue, 27 Jun 2017 17:01:54: 13000000 INFO @ Tue, 27 Jun 2017 17:01:54: 13000000 INFO @ Tue, 27 Jun 2017 17:01:54: 13000000 INFO @ Tue, 27 Jun 2017 17:02:03: 14000000 INFO @ Tue, 27 Jun 2017 17:02:03: 14000000 INFO @ Tue, 27 Jun 2017 17:02:03: 14000000 INFO @ Tue, 27 Jun 2017 17:02:11: 15000000 INFO @ Tue, 27 Jun 2017 17:02:11: 15000000 INFO @ Tue, 27 Jun 2017 17:02:11: 15000000 INFO @ Tue, 27 Jun 2017 17:02:19: 16000000 INFO @ Tue, 27 Jun 2017 17:02:19: 16000000 INFO @ Tue, 27 Jun 2017 17:02:20: 16000000 INFO @ Tue, 27 Jun 2017 17:02:28: 17000000 INFO @ Tue, 27 Jun 2017 17:02:28: 17000000 INFO @ Tue, 27 Jun 2017 17:02:28: 17000000 INFO @ Tue, 27 Jun 2017 17:02:36: 18000000 INFO @ Tue, 27 Jun 2017 17:02:36: 18000000 INFO @ Tue, 27 Jun 2017 17:02:37: 18000000 INFO @ Tue, 27 Jun 2017 17:02:44: 19000000 INFO @ Tue, 27 Jun 2017 17:02:44: 19000000 INFO @ Tue, 27 Jun 2017 17:02:45: 19000000 INFO @ Tue, 27 Jun 2017 17:02:53: 20000000 INFO @ Tue, 27 Jun 2017 17:02:53: 20000000 INFO @ Tue, 27 Jun 2017 17:02:53: 20000000 INFO @ Tue, 27 Jun 2017 17:03:01: 21000000 INFO @ Tue, 27 Jun 2017 17:03:01: 21000000 INFO @ Tue, 27 Jun 2017 17:03:02: 21000000 INFO @ Tue, 27 Jun 2017 17:03:06: #1 tag size is determined as 50 bps INFO @ Tue, 27 Jun 2017 17:03:06: #1 tag size = 50 INFO @ Tue, 27 Jun 2017 17:03:06: #1 total tags in treatment: 21578955 INFO @ Tue, 27 Jun 2017 17:03:06: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 17:03:06: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 17:03:06: #1 tags after filtering in treatment: 21578955 INFO @ Tue, 27 Jun 2017 17:03:06: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 17:03:06: #1 finished! INFO @ Tue, 27 Jun 2017 17:03:06: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 17:03:06: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 17:03:07: #1 tag size is determined as 50 bps INFO @ Tue, 27 Jun 2017 17:03:07: #1 tag size = 50 INFO @ Tue, 27 Jun 2017 17:03:07: #1 total tags in treatment: 21578955 INFO @ Tue, 27 Jun 2017 17:03:07: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 17:03:07: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 17:03:07: #1 tags after filtering in treatment: 21578955 INFO @ Tue, 27 Jun 2017 17:03:07: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 17:03:07: #1 finished! INFO @ Tue, 27 Jun 2017 17:03:07: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 17:03:07: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 17:03:08: #1 tag size is determined as 50 bps INFO @ Tue, 27 Jun 2017 17:03:08: #1 tag size = 50 INFO @ Tue, 27 Jun 2017 17:03:08: #1 total tags in treatment: 21578955 INFO @ Tue, 27 Jun 2017 17:03:08: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 17:03:08: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 17:03:08: #2 number of paired peaks: 69 WARNING @ Tue, 27 Jun 2017 17:03:08: Too few paired peaks (69) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 17:03:08: Process for pairing-model is terminated! cat: SRX1300691.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1300691.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1300691.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1300691.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Tue, 27 Jun 2017 17:03:08: #1 tags after filtering in treatment: 21578955 INFO @ Tue, 27 Jun 2017 17:03:08: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 17:03:08: #1 finished! INFO @ Tue, 27 Jun 2017 17:03:08: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 17:03:08: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 17:03:09: #2 number of paired peaks: 69 WARNING @ Tue, 27 Jun 2017 17:03:09: Too few paired peaks (69) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 17:03:09: Process for pairing-model is terminated! cat: SRX1300691.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 6 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1300691.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1300691.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1300691.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Tue, 27 Jun 2017 17:03:10: #2 number of paired peaks: 69 WARNING @ Tue, 27 Jun 2017 17:03:10: Too few paired peaks (69) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 17:03:10: Process for pairing-model is terminated! cat: SRX1300691.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1300691.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1300691.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1300691.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。