Job ID = 16438202 SRX = SRX12261370 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 41368748 spots for SRR15972626/SRR15972626.sra Written 41368748 spots for SRR15972626/SRR15972626.sra fastq に変換しました。 bowtie でマッピング中... Your job 16439356 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 01:04:36 41368748 reads; of these: 41368748 (100.00%) were paired; of these: 7148800 (17.28%) aligned concordantly 0 times 24347771 (58.86%) aligned concordantly exactly 1 time 9872177 (23.86%) aligned concordantly >1 times ---- 7148800 pairs aligned concordantly 0 times; of these: 162956 (2.28%) aligned discordantly 1 time ---- 6985844 pairs aligned 0 times concordantly or discordantly; of these: 13971688 mates make up the pairs; of these: 11803951 (84.48%) aligned 0 times 1003462 (7.18%) aligned exactly 1 time 1164275 (8.33%) aligned >1 times 85.73% overall alignment rate Time searching: 01:04:36 Overall time: 01:04:36 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 32 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 8313494 / 22861008 = 0.3637 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 15:12:33: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX12261370/SRX12261370.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX12261370/SRX12261370.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX12261370/SRX12261370.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX12261370/SRX12261370.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 15:12:33: #1 read tag files... INFO @ Tue, 02 Aug 2022 15:12:33: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 15:12:39: 1000000 INFO @ Tue, 02 Aug 2022 15:12:46: 2000000 INFO @ Tue, 02 Aug 2022 15:12:53: 3000000 INFO @ Tue, 02 Aug 2022 15:12:59: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 15:13:03: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX12261370/SRX12261370.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX12261370/SRX12261370.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX12261370/SRX12261370.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX12261370/SRX12261370.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 15:13:03: #1 read tag files... INFO @ Tue, 02 Aug 2022 15:13:03: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 15:13:06: 5000000 INFO @ Tue, 02 Aug 2022 15:13:09: 1000000 INFO @ Tue, 02 Aug 2022 15:13:13: 6000000 INFO @ Tue, 02 Aug 2022 15:13:16: 2000000 INFO @ Tue, 02 Aug 2022 15:13:20: 7000000 INFO @ Tue, 02 Aug 2022 15:13:22: 3000000 INFO @ Tue, 02 Aug 2022 15:13:27: 8000000 INFO @ Tue, 02 Aug 2022 15:13:28: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 15:13:33: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX12261370/SRX12261370.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX12261370/SRX12261370.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX12261370/SRX12261370.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX12261370/SRX12261370.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 15:13:33: #1 read tag files... INFO @ Tue, 02 Aug 2022 15:13:33: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 15:13:34: 9000000 INFO @ Tue, 02 Aug 2022 15:13:35: 5000000 INFO @ Tue, 02 Aug 2022 15:13:40: 1000000 INFO @ Tue, 02 Aug 2022 15:13:41: 10000000 INFO @ Tue, 02 Aug 2022 15:13:41: 6000000 INFO @ Tue, 02 Aug 2022 15:13:47: 2000000 INFO @ Tue, 02 Aug 2022 15:13:47: 7000000 INFO @ Tue, 02 Aug 2022 15:13:48: 11000000 INFO @ Tue, 02 Aug 2022 15:13:54: 3000000 INFO @ Tue, 02 Aug 2022 15:13:54: 8000000 INFO @ Tue, 02 Aug 2022 15:13:55: 12000000 INFO @ Tue, 02 Aug 2022 15:14:00: 9000000 INFO @ Tue, 02 Aug 2022 15:14:01: 4000000 INFO @ Tue, 02 Aug 2022 15:14:02: 13000000 INFO @ Tue, 02 Aug 2022 15:14:07: 10000000 INFO @ Tue, 02 Aug 2022 15:14:07: 5000000 INFO @ Tue, 02 Aug 2022 15:14:09: 14000000 INFO @ Tue, 02 Aug 2022 15:14:14: 11000000 INFO @ Tue, 02 Aug 2022 15:14:14: 6000000 INFO @ Tue, 02 Aug 2022 15:14:16: 15000000 INFO @ Tue, 02 Aug 2022 15:14:20: 12000000 INFO @ Tue, 02 Aug 2022 15:14:21: 7000000 INFO @ Tue, 02 Aug 2022 15:14:23: 16000000 INFO @ Tue, 02 Aug 2022 15:14:26: 13000000 INFO @ Tue, 02 Aug 2022 15:14:28: 8000000 INFO @ Tue, 02 Aug 2022 15:14:30: 17000000 INFO @ Tue, 02 Aug 2022 15:14:33: 14000000 INFO @ Tue, 02 Aug 2022 15:14:35: 9000000 INFO @ Tue, 02 Aug 2022 15:14:37: 18000000 INFO @ Tue, 02 Aug 2022 15:14:39: 15000000 INFO @ Tue, 02 Aug 2022 15:14:42: 10000000 INFO @ Tue, 02 Aug 2022 15:14:44: 19000000 INFO @ Tue, 02 Aug 2022 15:14:45: 16000000 INFO @ Tue, 02 Aug 2022 15:14:48: 11000000 INFO @ Tue, 02 Aug 2022 15:14:51: 20000000 INFO @ Tue, 02 Aug 2022 15:14:52: 17000000 INFO @ Tue, 02 Aug 2022 15:14:55: 12000000 INFO @ Tue, 02 Aug 2022 15:14:58: 21000000 INFO @ Tue, 02 Aug 2022 15:14:58: 18000000 INFO @ Tue, 02 Aug 2022 15:15:02: 13000000 INFO @ Tue, 02 Aug 2022 15:15:04: 22000000 INFO @ Tue, 02 Aug 2022 15:15:04: 19000000 INFO @ Tue, 02 Aug 2022 15:15:09: 14000000 INFO @ Tue, 02 Aug 2022 15:15:11: 20000000 INFO @ Tue, 02 Aug 2022 15:15:11: 23000000 INFO @ Tue, 02 Aug 2022 15:15:16: 15000000 INFO @ Tue, 02 Aug 2022 15:15:17: 21000000 INFO @ Tue, 02 Aug 2022 15:15:18: 24000000 INFO @ Tue, 02 Aug 2022 15:15:22: 16000000 INFO @ Tue, 02 Aug 2022 15:15:24: 22000000 INFO @ Tue, 02 Aug 2022 15:15:25: 25000000 INFO @ Tue, 02 Aug 2022 15:15:29: 17000000 INFO @ Tue, 02 Aug 2022 15:15:30: 23000000 INFO @ Tue, 02 Aug 2022 15:15:32: 26000000 INFO @ Tue, 02 Aug 2022 15:15:36: 18000000 INFO @ Tue, 02 Aug 2022 15:15:36: 24000000 INFO @ Tue, 02 Aug 2022 15:15:39: 27000000 INFO @ Tue, 02 Aug 2022 15:15:42: 25000000 INFO @ Tue, 02 Aug 2022 15:15:43: 19000000 INFO @ Tue, 02 Aug 2022 15:15:46: 28000000 INFO @ Tue, 02 Aug 2022 15:15:49: 26000000 INFO @ Tue, 02 Aug 2022 15:15:50: 20000000 INFO @ Tue, 02 Aug 2022 15:15:53: 29000000 INFO @ Tue, 02 Aug 2022 15:15:55: 27000000 INFO @ Tue, 02 Aug 2022 15:15:57: 21000000 INFO @ Tue, 02 Aug 2022 15:15:59: 30000000 INFO @ Tue, 02 Aug 2022 15:16:01: 28000000 INFO @ Tue, 02 Aug 2022 15:16:03: 22000000 INFO @ Tue, 02 Aug 2022 15:16:06: 31000000 INFO @ Tue, 02 Aug 2022 15:16:08: 29000000 INFO @ Tue, 02 Aug 2022 15:16:10: 23000000 INFO @ Tue, 02 Aug 2022 15:16:13: 32000000 INFO @ Tue, 02 Aug 2022 15:16:14: 30000000 INFO @ Tue, 02 Aug 2022 15:16:17: 24000000 INFO @ Tue, 02 Aug 2022 15:16:20: 33000000 INFO @ Tue, 02 Aug 2022 15:16:20: 31000000 INFO @ Tue, 02 Aug 2022 15:16:24: 25000000 INFO @ Tue, 02 Aug 2022 15:16:27: 32000000 INFO @ Tue, 02 Aug 2022 15:16:27: 34000000 INFO @ Tue, 02 Aug 2022 15:16:31: 26000000 INFO @ Tue, 02 Aug 2022 15:16:33: 33000000 INFO @ Tue, 02 Aug 2022 15:16:34: 35000000 INFO @ Tue, 02 Aug 2022 15:16:37: 27000000 INFO @ Tue, 02 Aug 2022 15:16:39: 34000000 INFO @ Tue, 02 Aug 2022 15:16:41: 36000000 INFO @ Tue, 02 Aug 2022 15:16:44: 28000000 INFO @ Tue, 02 Aug 2022 15:16:46: 35000000 INFO @ Tue, 02 Aug 2022 15:16:48: 37000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 02 Aug 2022 15:16:51: 29000000 INFO @ Tue, 02 Aug 2022 15:16:52: 36000000 INFO @ Tue, 02 Aug 2022 15:16:54: 38000000 INFO @ Tue, 02 Aug 2022 15:16:58: 30000000 INFO @ Tue, 02 Aug 2022 15:16:58: 37000000 INFO @ Tue, 02 Aug 2022 15:17:01: 39000000 INFO @ Tue, 02 Aug 2022 15:17:05: 38000000 INFO @ Tue, 02 Aug 2022 15:17:05: 31000000 INFO @ Tue, 02 Aug 2022 15:17:08: 40000000 INFO @ Tue, 02 Aug 2022 15:17:11: 39000000 INFO @ Tue, 02 Aug 2022 15:17:12: 32000000 INFO @ Tue, 02 Aug 2022 15:17:15: 41000000 INFO @ Tue, 02 Aug 2022 15:17:17: 40000000 INFO @ Tue, 02 Aug 2022 15:17:19: 33000000 INFO @ Tue, 02 Aug 2022 15:17:22: 42000000 INFO @ Tue, 02 Aug 2022 15:17:24: 41000000 INFO @ Tue, 02 Aug 2022 15:17:26: 34000000 INFO @ Tue, 02 Aug 2022 15:17:28: 43000000 INFO @ Tue, 02 Aug 2022 15:17:30: 42000000 INFO @ Tue, 02 Aug 2022 15:17:32: 35000000 INFO @ Tue, 02 Aug 2022 15:17:35: 44000000 INFO @ Tue, 02 Aug 2022 15:17:36: 43000000 INFO @ Tue, 02 Aug 2022 15:17:39: 36000000 BigWig に変換しました。 INFO @ Tue, 02 Aug 2022 15:17:42: 44000000 INFO @ Tue, 02 Aug 2022 15:17:43: 45000000 INFO @ Tue, 02 Aug 2022 15:17:47: 37000000 INFO @ Tue, 02 Aug 2022 15:17:49: 45000000 INFO @ Tue, 02 Aug 2022 15:17:50: 46000000 INFO @ Tue, 02 Aug 2022 15:17:54: 38000000 INFO @ Tue, 02 Aug 2022 15:17:56: 46000000 INFO @ Tue, 02 Aug 2022 15:17:58: 47000000 INFO @ Tue, 02 Aug 2022 15:18:01: 39000000 INFO @ Tue, 02 Aug 2022 15:18:03: 47000000 INFO @ Tue, 02 Aug 2022 15:18:05: 48000000 INFO @ Tue, 02 Aug 2022 15:18:08: 40000000 INFO @ Tue, 02 Aug 2022 15:18:09: 48000000 INFO @ Tue, 02 Aug 2022 15:18:12: 49000000 INFO @ Tue, 02 Aug 2022 15:18:15: 41000000 INFO @ Tue, 02 Aug 2022 15:18:16: 49000000 INFO @ Tue, 02 Aug 2022 15:18:19: 50000000 INFO @ Tue, 02 Aug 2022 15:18:22: 42000000 INFO @ Tue, 02 Aug 2022 15:18:23: 50000000 INFO @ Tue, 02 Aug 2022 15:18:26: 51000000 INFO @ Tue, 02 Aug 2022 15:18:29: 43000000 INFO @ Tue, 02 Aug 2022 15:18:29: 51000000 INFO @ Tue, 02 Aug 2022 15:18:34: 52000000 INFO @ Tue, 02 Aug 2022 15:18:36: 52000000 INFO @ Tue, 02 Aug 2022 15:18:36: 44000000 INFO @ Tue, 02 Aug 2022 15:18:41: 53000000 INFO @ Tue, 02 Aug 2022 15:18:43: 53000000 INFO @ Tue, 02 Aug 2022 15:18:44: 45000000 INFO @ Tue, 02 Aug 2022 15:18:48: 54000000 INFO @ Tue, 02 Aug 2022 15:18:49: 54000000 INFO @ Tue, 02 Aug 2022 15:18:50: #1 tag size is determined as 67 bps INFO @ Tue, 02 Aug 2022 15:18:50: #1 tag size = 67 INFO @ Tue, 02 Aug 2022 15:18:50: #1 total tags in treatment: 25911543 INFO @ Tue, 02 Aug 2022 15:18:50: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 15:18:50: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 15:18:51: 46000000 INFO @ Tue, 02 Aug 2022 15:18:51: #1 tags after filtering in treatment: 14901294 INFO @ Tue, 02 Aug 2022 15:18:51: #1 Redundant rate of treatment: 0.42 INFO @ Tue, 02 Aug 2022 15:18:51: #1 finished! INFO @ Tue, 02 Aug 2022 15:18:51: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 15:18:51: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 15:18:52: #1 tag size is determined as 67 bps INFO @ Tue, 02 Aug 2022 15:18:52: #1 tag size = 67 INFO @ Tue, 02 Aug 2022 15:18:52: #1 total tags in treatment: 25911543 INFO @ Tue, 02 Aug 2022 15:18:52: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 15:18:52: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 15:18:52: #2 number of paired peaks: 879 WARNING @ Tue, 02 Aug 2022 15:18:52: Fewer paired peaks (879) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 879 pairs to build model! INFO @ Tue, 02 Aug 2022 15:18:52: start model_add_line... INFO @ Tue, 02 Aug 2022 15:18:52: #1 tags after filtering in treatment: 14901294 INFO @ Tue, 02 Aug 2022 15:18:52: #1 Redundant rate of treatment: 0.42 INFO @ Tue, 02 Aug 2022 15:18:52: #1 finished! INFO @ Tue, 02 Aug 2022 15:18:52: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 15:18:52: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 15:18:52: start X-correlation... INFO @ Tue, 02 Aug 2022 15:18:52: end of X-cor INFO @ Tue, 02 Aug 2022 15:18:52: #2 finished! INFO @ Tue, 02 Aug 2022 15:18:52: #2 predicted fragment length is 62 bps INFO @ Tue, 02 Aug 2022 15:18:52: #2 alternative fragment length(s) may be 62 bps INFO @ Tue, 02 Aug 2022 15:18:52: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX12261370/SRX12261370.05_model.r WARNING @ Tue, 02 Aug 2022 15:18:52: #2 Since the d (62) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 02 Aug 2022 15:18:52: #2 You may need to consider one of the other alternative d(s): 62 WARNING @ Tue, 02 Aug 2022 15:18:52: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 02 Aug 2022 15:18:52: #3 Call peaks... INFO @ Tue, 02 Aug 2022 15:18:52: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 02 Aug 2022 15:18:53: #2 number of paired peaks: 879 WARNING @ Tue, 02 Aug 2022 15:18:53: Fewer paired peaks (879) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 879 pairs to build model! INFO @ Tue, 02 Aug 2022 15:18:53: start model_add_line... INFO @ Tue, 02 Aug 2022 15:18:53: start X-correlation... INFO @ Tue, 02 Aug 2022 15:18:53: end of X-cor INFO @ Tue, 02 Aug 2022 15:18:53: #2 finished! INFO @ Tue, 02 Aug 2022 15:18:53: #2 predicted fragment length is 62 bps INFO @ Tue, 02 Aug 2022 15:18:53: #2 alternative fragment length(s) may be 62 bps INFO @ Tue, 02 Aug 2022 15:18:53: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX12261370/SRX12261370.10_model.r WARNING @ Tue, 02 Aug 2022 15:18:53: #2 Since the d (62) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 02 Aug 2022 15:18:53: #2 You may need to consider one of the other alternative d(s): 62 WARNING @ Tue, 02 Aug 2022 15:18:53: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 02 Aug 2022 15:18:53: #3 Call peaks... INFO @ Tue, 02 Aug 2022 15:18:53: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 02 Aug 2022 15:18:57: 47000000 INFO @ Tue, 02 Aug 2022 15:19:04: 48000000 INFO @ Tue, 02 Aug 2022 15:19:10: 49000000 INFO @ Tue, 02 Aug 2022 15:19:16: 50000000 INFO @ Tue, 02 Aug 2022 15:19:20: #3 Call peaks for each chromosome... INFO @ Tue, 02 Aug 2022 15:19:21: #3 Call peaks for each chromosome... INFO @ Tue, 02 Aug 2022 15:19:22: 51000000 INFO @ Tue, 02 Aug 2022 15:19:28: 52000000 INFO @ Tue, 02 Aug 2022 15:19:34: 53000000 INFO @ Tue, 02 Aug 2022 15:19:34: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX12261370/SRX12261370.05_peaks.xls INFO @ Tue, 02 Aug 2022 15:19:35: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX12261370/SRX12261370.05_peaks.narrowPeak INFO @ Tue, 02 Aug 2022 15:19:35: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX12261370/SRX12261370.05_summits.bed INFO @ Tue, 02 Aug 2022 15:19:35: Done! pass1 - making usageList (14 chroms): 3 millis pass2 - checking and writing primary data (18824 records, 4 fields): 44 millis CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 15:19:35: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX12261370/SRX12261370.10_peaks.xls INFO @ Tue, 02 Aug 2022 15:19:36: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX12261370/SRX12261370.10_peaks.narrowPeak INFO @ Tue, 02 Aug 2022 15:19:36: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX12261370/SRX12261370.10_summits.bed INFO @ Tue, 02 Aug 2022 15:19:36: Done! pass1 - making usageList (13 chroms): 2 millis pass2 - checking and writing primary data (9445 records, 4 fields): 18 millis CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 15:19:40: 54000000 INFO @ Tue, 02 Aug 2022 15:19:43: #1 tag size is determined as 67 bps INFO @ Tue, 02 Aug 2022 15:19:43: #1 tag size = 67 INFO @ Tue, 02 Aug 2022 15:19:43: #1 total tags in treatment: 25911543 INFO @ Tue, 02 Aug 2022 15:19:43: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 15:19:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 15:19:43: #1 tags after filtering in treatment: 14901294 INFO @ Tue, 02 Aug 2022 15:19:43: #1 Redundant rate of treatment: 0.42 INFO @ Tue, 02 Aug 2022 15:19:43: #1 finished! INFO @ Tue, 02 Aug 2022 15:19:43: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 15:19:43: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 15:19:44: #2 number of paired peaks: 879 WARNING @ Tue, 02 Aug 2022 15:19:44: Fewer paired peaks (879) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 879 pairs to build model! INFO @ Tue, 02 Aug 2022 15:19:44: start model_add_line... INFO @ Tue, 02 Aug 2022 15:19:44: start X-correlation... INFO @ Tue, 02 Aug 2022 15:19:44: end of X-cor INFO @ Tue, 02 Aug 2022 15:19:44: #2 finished! INFO @ Tue, 02 Aug 2022 15:19:44: #2 predicted fragment length is 62 bps INFO @ Tue, 02 Aug 2022 15:19:44: #2 alternative fragment length(s) may be 62 bps INFO @ Tue, 02 Aug 2022 15:19:44: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX12261370/SRX12261370.20_model.r WARNING @ Tue, 02 Aug 2022 15:19:44: #2 Since the d (62) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 02 Aug 2022 15:19:44: #2 You may need to consider one of the other alternative d(s): 62 WARNING @ Tue, 02 Aug 2022 15:19:44: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 02 Aug 2022 15:19:44: #3 Call peaks... INFO @ Tue, 02 Aug 2022 15:19:44: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 02 Aug 2022 15:20:13: #3 Call peaks for each chromosome... INFO @ Tue, 02 Aug 2022 15:20:27: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX12261370/SRX12261370.20_peaks.xls INFO @ Tue, 02 Aug 2022 15:20:27: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX12261370/SRX12261370.20_peaks.narrowPeak INFO @ Tue, 02 Aug 2022 15:20:27: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX12261370/SRX12261370.20_summits.bed INFO @ Tue, 02 Aug 2022 15:20:27: Done! pass1 - making usageList (13 chroms): 1 millis pass2 - checking and writing primary data (2242 records, 4 fields): 32 millis CompletedMACS2peakCalling