Job ID = 16437903 SRX = SRX12261337 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 20513815 spots for SRR15972593/SRR15972593.sra Written 20513815 spots for SRR15972593/SRR15972593.sra fastq に変換しました。 bowtie でマッピング中... Your job 16438092 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:14:59 20513815 reads; of these: 20513815 (100.00%) were unpaired; of these: 696012 (3.39%) aligned 0 times 12327015 (60.09%) aligned exactly 1 time 7490788 (36.52%) aligned >1 times 96.61% overall alignment rate Time searching: 00:14:59 Overall time: 00:14:59 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 4019962 / 19817803 = 0.2028 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 13:36:42: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX12261337/SRX12261337.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX12261337/SRX12261337.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX12261337/SRX12261337.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX12261337/SRX12261337.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 13:36:42: #1 read tag files... INFO @ Tue, 02 Aug 2022 13:36:42: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 13:36:50: 1000000 INFO @ Tue, 02 Aug 2022 13:36:57: 2000000 INFO @ Tue, 02 Aug 2022 13:37:04: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 13:37:11: 4000000 INFO @ Tue, 02 Aug 2022 13:37:11: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX12261337/SRX12261337.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX12261337/SRX12261337.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX12261337/SRX12261337.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX12261337/SRX12261337.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 13:37:11: #1 read tag files... INFO @ Tue, 02 Aug 2022 13:37:11: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 13:37:18: 1000000 INFO @ Tue, 02 Aug 2022 13:37:18: 5000000 INFO @ Tue, 02 Aug 2022 13:37:24: 2000000 INFO @ Tue, 02 Aug 2022 13:37:25: 6000000 INFO @ Tue, 02 Aug 2022 13:37:31: 3000000 INFO @ Tue, 02 Aug 2022 13:37:33: 7000000 INFO @ Tue, 02 Aug 2022 13:37:37: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 13:37:40: 8000000 INFO @ Tue, 02 Aug 2022 13:37:41: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX12261337/SRX12261337.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX12261337/SRX12261337.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX12261337/SRX12261337.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX12261337/SRX12261337.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 13:37:41: #1 read tag files... INFO @ Tue, 02 Aug 2022 13:37:41: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 13:37:43: 5000000 INFO @ Tue, 02 Aug 2022 13:37:47: 9000000 INFO @ Tue, 02 Aug 2022 13:37:48: 1000000 INFO @ Tue, 02 Aug 2022 13:37:50: 6000000 INFO @ Tue, 02 Aug 2022 13:37:54: 2000000 INFO @ Tue, 02 Aug 2022 13:37:55: 10000000 INFO @ Tue, 02 Aug 2022 13:37:56: 7000000 INFO @ Tue, 02 Aug 2022 13:38:01: 3000000 INFO @ Tue, 02 Aug 2022 13:38:02: 11000000 INFO @ Tue, 02 Aug 2022 13:38:03: 8000000 INFO @ Tue, 02 Aug 2022 13:38:08: 4000000 INFO @ Tue, 02 Aug 2022 13:38:09: 9000000 INFO @ Tue, 02 Aug 2022 13:38:10: 12000000 INFO @ Tue, 02 Aug 2022 13:38:14: 5000000 INFO @ Tue, 02 Aug 2022 13:38:15: 10000000 INFO @ Tue, 02 Aug 2022 13:38:18: 13000000 INFO @ Tue, 02 Aug 2022 13:38:21: 6000000 INFO @ Tue, 02 Aug 2022 13:38:22: 11000000 INFO @ Tue, 02 Aug 2022 13:38:26: 14000000 INFO @ Tue, 02 Aug 2022 13:38:27: 7000000 INFO @ Tue, 02 Aug 2022 13:38:29: 12000000 INFO @ Tue, 02 Aug 2022 13:38:34: 15000000 INFO @ Tue, 02 Aug 2022 13:38:34: 8000000 INFO @ Tue, 02 Aug 2022 13:38:35: 13000000 INFO @ Tue, 02 Aug 2022 13:38:40: #1 tag size is determined as 74 bps INFO @ Tue, 02 Aug 2022 13:38:40: #1 tag size = 74 INFO @ Tue, 02 Aug 2022 13:38:40: #1 total tags in treatment: 15797841 INFO @ Tue, 02 Aug 2022 13:38:40: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 13:38:40: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 13:38:40: #1 tags after filtering in treatment: 15797841 INFO @ Tue, 02 Aug 2022 13:38:40: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 13:38:40: #1 finished! INFO @ Tue, 02 Aug 2022 13:38:40: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 13:38:40: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 13:38:40: 9000000 INFO @ Tue, 02 Aug 2022 13:38:41: #2 number of paired peaks: 84 WARNING @ Tue, 02 Aug 2022 13:38:41: Too few paired peaks (84) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 13:38:41: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX12261337/SRX12261337.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX12261337/SRX12261337.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX12261337/SRX12261337.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX12261337/SRX12261337.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 13:38:42: 14000000 INFO @ Tue, 02 Aug 2022 13:38:47: 10000000 INFO @ Tue, 02 Aug 2022 13:38:48: 15000000 INFO @ Tue, 02 Aug 2022 13:38:53: 11000000 INFO @ Tue, 02 Aug 2022 13:38:53: #1 tag size is determined as 74 bps INFO @ Tue, 02 Aug 2022 13:38:53: #1 tag size = 74 INFO @ Tue, 02 Aug 2022 13:38:53: #1 total tags in treatment: 15797841 INFO @ Tue, 02 Aug 2022 13:38:53: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 13:38:53: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 13:38:53: #1 tags after filtering in treatment: 15797841 INFO @ Tue, 02 Aug 2022 13:38:53: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 13:38:53: #1 finished! INFO @ Tue, 02 Aug 2022 13:38:53: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 13:38:53: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 13:38:54: #2 number of paired peaks: 84 WARNING @ Tue, 02 Aug 2022 13:38:54: Too few paired peaks (84) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 13:38:54: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX12261337/SRX12261337.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX12261337/SRX12261337.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX12261337/SRX12261337.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX12261337/SRX12261337.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 13:38:59: 12000000 INFO @ Tue, 02 Aug 2022 13:39:05: 13000000 INFO @ Tue, 02 Aug 2022 13:39:11: 14000000 INFO @ Tue, 02 Aug 2022 13:39:17: 15000000 INFO @ Tue, 02 Aug 2022 13:39:22: #1 tag size is determined as 74 bps INFO @ Tue, 02 Aug 2022 13:39:22: #1 tag size = 74 INFO @ Tue, 02 Aug 2022 13:39:22: #1 total tags in treatment: 15797841 INFO @ Tue, 02 Aug 2022 13:39:22: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 13:39:22: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 13:39:22: #1 tags after filtering in treatment: 15797841 INFO @ Tue, 02 Aug 2022 13:39:22: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 13:39:22: #1 finished! INFO @ Tue, 02 Aug 2022 13:39:22: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 13:39:22: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 13:39:23: #2 number of paired peaks: 84 WARNING @ Tue, 02 Aug 2022 13:39:23: Too few paired peaks (84) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 13:39:23: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX12261337/SRX12261337.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX12261337/SRX12261337.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX12261337/SRX12261337.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX12261337/SRX12261337.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。