Job ID = 16437902 SRX = SRX12261336 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 26865630 spots for SRR15972592/SRR15972592.sra Written 26865630 spots for SRR15972592/SRR15972592.sra fastq に変換しました。 bowtie でマッピング中... Your job 16438103 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:01 Multiseed full-index search: 00:17:37 26865630 reads; of these: 26865630 (100.00%) were unpaired; of these: 1008935 (3.76%) aligned 0 times 17860321 (66.48%) aligned exactly 1 time 7996374 (29.76%) aligned >1 times 96.24% overall alignment rate Time searching: 00:17:38 Overall time: 00:17:38 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 13014936 / 25856695 = 0.5033 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 13:39:32: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX12261336/SRX12261336.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX12261336/SRX12261336.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX12261336/SRX12261336.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX12261336/SRX12261336.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 13:39:32: #1 read tag files... INFO @ Tue, 02 Aug 2022 13:39:32: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 13:39:40: 1000000 INFO @ Tue, 02 Aug 2022 13:39:49: 2000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 13:39:58: 3000000 INFO @ Tue, 02 Aug 2022 13:40:02: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX12261336/SRX12261336.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX12261336/SRX12261336.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX12261336/SRX12261336.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX12261336/SRX12261336.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 13:40:02: #1 read tag files... INFO @ Tue, 02 Aug 2022 13:40:02: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 13:40:08: 4000000 INFO @ Tue, 02 Aug 2022 13:40:09: 1000000 INFO @ Tue, 02 Aug 2022 13:40:16: 5000000 INFO @ Tue, 02 Aug 2022 13:40:17: 2000000 INFO @ Tue, 02 Aug 2022 13:40:24: 3000000 INFO @ Tue, 02 Aug 2022 13:40:25: 6000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 13:40:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX12261336/SRX12261336.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX12261336/SRX12261336.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX12261336/SRX12261336.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX12261336/SRX12261336.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 13:40:30: #1 read tag files... INFO @ Tue, 02 Aug 2022 13:40:30: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 13:40:32: 4000000 INFO @ Tue, 02 Aug 2022 13:40:34: 7000000 INFO @ Tue, 02 Aug 2022 13:40:39: 5000000 INFO @ Tue, 02 Aug 2022 13:40:40: 1000000 INFO @ Tue, 02 Aug 2022 13:40:44: 8000000 INFO @ Tue, 02 Aug 2022 13:40:47: 6000000 INFO @ Tue, 02 Aug 2022 13:40:50: 2000000 INFO @ Tue, 02 Aug 2022 13:40:54: 9000000 INFO @ Tue, 02 Aug 2022 13:40:55: 7000000 INFO @ Tue, 02 Aug 2022 13:41:00: 3000000 INFO @ Tue, 02 Aug 2022 13:41:03: 8000000 INFO @ Tue, 02 Aug 2022 13:41:04: 10000000 INFO @ Tue, 02 Aug 2022 13:41:08: 4000000 INFO @ Tue, 02 Aug 2022 13:41:11: 9000000 INFO @ Tue, 02 Aug 2022 13:41:15: 11000000 INFO @ Tue, 02 Aug 2022 13:41:17: 5000000 INFO @ Tue, 02 Aug 2022 13:41:19: 10000000 INFO @ Tue, 02 Aug 2022 13:41:25: 12000000 INFO @ Tue, 02 Aug 2022 13:41:27: 11000000 INFO @ Tue, 02 Aug 2022 13:41:27: 6000000 INFO @ Tue, 02 Aug 2022 13:41:34: #1 tag size is determined as 74 bps INFO @ Tue, 02 Aug 2022 13:41:34: #1 tag size = 74 INFO @ Tue, 02 Aug 2022 13:41:34: #1 total tags in treatment: 12841759 INFO @ Tue, 02 Aug 2022 13:41:34: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 13:41:34: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 13:41:34: #1 tags after filtering in treatment: 12841759 INFO @ Tue, 02 Aug 2022 13:41:34: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 13:41:34: #1 finished! INFO @ Tue, 02 Aug 2022 13:41:34: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 13:41:34: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 13:41:34: 12000000 INFO @ Tue, 02 Aug 2022 13:41:35: #2 number of paired peaks: 88 WARNING @ Tue, 02 Aug 2022 13:41:35: Too few paired peaks (88) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 13:41:35: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX12261336/SRX12261336.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 12 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX12261336/SRX12261336.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX12261336/SRX12261336.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX12261336/SRX12261336.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 13:41:36: 7000000 INFO @ Tue, 02 Aug 2022 13:41:41: #1 tag size is determined as 74 bps INFO @ Tue, 02 Aug 2022 13:41:41: #1 tag size = 74 INFO @ Tue, 02 Aug 2022 13:41:41: #1 total tags in treatment: 12841759 INFO @ Tue, 02 Aug 2022 13:41:41: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 13:41:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 13:41:41: #1 tags after filtering in treatment: 12841759 INFO @ Tue, 02 Aug 2022 13:41:41: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 13:41:41: #1 finished! INFO @ Tue, 02 Aug 2022 13:41:41: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 13:41:41: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 13:41:42: #2 number of paired peaks: 88 WARNING @ Tue, 02 Aug 2022 13:41:42: Too few paired peaks (88) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 13:41:42: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX12261336/SRX12261336.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX12261336/SRX12261336.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX12261336/SRX12261336.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX12261336/SRX12261336.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 13:41:45: 8000000 INFO @ Tue, 02 Aug 2022 13:41:53: 9000000 INFO @ Tue, 02 Aug 2022 13:42:01: 10000000 INFO @ Tue, 02 Aug 2022 13:42:09: 11000000 INFO @ Tue, 02 Aug 2022 13:42:17: 12000000 INFO @ Tue, 02 Aug 2022 13:42:24: #1 tag size is determined as 74 bps INFO @ Tue, 02 Aug 2022 13:42:24: #1 tag size = 74 INFO @ Tue, 02 Aug 2022 13:42:24: #1 total tags in treatment: 12841759 INFO @ Tue, 02 Aug 2022 13:42:24: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 13:42:24: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 13:42:24: #1 tags after filtering in treatment: 12841759 INFO @ Tue, 02 Aug 2022 13:42:24: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 13:42:24: #1 finished! INFO @ Tue, 02 Aug 2022 13:42:24: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 13:42:24: #2 looking for paired plus/minus strand peaks... BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 02 Aug 2022 13:42:25: #2 number of paired peaks: 88 WARNING @ Tue, 02 Aug 2022 13:42:25: Too few paired peaks (88) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 13:42:25: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX12261336/SRX12261336.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX12261336/SRX12261336.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX12261336/SRX12261336.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX12261336/SRX12261336.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。