Job ID = 16437635 SRX = SRX12261327 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 22409622 spots for SRR15972583/SRR15972583.sra Written 22409622 spots for SRR15972583/SRR15972583.sra fastq に変換しました。 bowtie でマッピング中... Your job 16438055 ("srTdm6") has been submitted Time loading reference: 00:00:01 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:13:01 22409622 reads; of these: 22409622 (100.00%) were unpaired; of these: 844758 (3.77%) aligned 0 times 13115800 (58.53%) aligned exactly 1 time 8449064 (37.70%) aligned >1 times 96.23% overall alignment rate Time searching: 00:13:02 Overall time: 00:13:02 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 5847928 / 21564864 = 0.2712 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 13:23:23: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX12261327/SRX12261327.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX12261327/SRX12261327.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX12261327/SRX12261327.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX12261327/SRX12261327.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 13:23:23: #1 read tag files... INFO @ Tue, 02 Aug 2022 13:23:23: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 13:23:29: 1000000 INFO @ Tue, 02 Aug 2022 13:23:36: 2000000 INFO @ Tue, 02 Aug 2022 13:23:43: 3000000 INFO @ Tue, 02 Aug 2022 13:23:49: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 13:23:52: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX12261327/SRX12261327.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX12261327/SRX12261327.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX12261327/SRX12261327.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX12261327/SRX12261327.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 13:23:52: #1 read tag files... INFO @ Tue, 02 Aug 2022 13:23:52: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 13:23:57: 5000000 INFO @ Tue, 02 Aug 2022 13:24:01: 1000000 INFO @ Tue, 02 Aug 2022 13:24:06: 6000000 INFO @ Tue, 02 Aug 2022 13:24:09: 2000000 INFO @ Tue, 02 Aug 2022 13:24:14: 7000000 INFO @ Tue, 02 Aug 2022 13:24:17: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 13:24:22: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX12261327/SRX12261327.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX12261327/SRX12261327.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX12261327/SRX12261327.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX12261327/SRX12261327.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 13:24:22: #1 read tag files... INFO @ Tue, 02 Aug 2022 13:24:22: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 13:24:23: 8000000 INFO @ Tue, 02 Aug 2022 13:24:26: 4000000 INFO @ Tue, 02 Aug 2022 13:24:31: 1000000 INFO @ Tue, 02 Aug 2022 13:24:32: 9000000 INFO @ Tue, 02 Aug 2022 13:24:35: 5000000 INFO @ Tue, 02 Aug 2022 13:24:39: 2000000 INFO @ Tue, 02 Aug 2022 13:24:40: 10000000 INFO @ Tue, 02 Aug 2022 13:24:43: 6000000 INFO @ Tue, 02 Aug 2022 13:24:48: 3000000 INFO @ Tue, 02 Aug 2022 13:24:49: 11000000 INFO @ Tue, 02 Aug 2022 13:24:52: 7000000 INFO @ Tue, 02 Aug 2022 13:24:56: 4000000 INFO @ Tue, 02 Aug 2022 13:24:57: 12000000 INFO @ Tue, 02 Aug 2022 13:25:01: 8000000 INFO @ Tue, 02 Aug 2022 13:25:05: 5000000 INFO @ Tue, 02 Aug 2022 13:25:06: 13000000 INFO @ Tue, 02 Aug 2022 13:25:09: 9000000 INFO @ Tue, 02 Aug 2022 13:25:14: 6000000 INFO @ Tue, 02 Aug 2022 13:25:14: 14000000 INFO @ Tue, 02 Aug 2022 13:25:19: 10000000 INFO @ Tue, 02 Aug 2022 13:25:22: 7000000 INFO @ Tue, 02 Aug 2022 13:25:23: 15000000 INFO @ Tue, 02 Aug 2022 13:25:28: 11000000 INFO @ Tue, 02 Aug 2022 13:25:29: #1 tag size is determined as 74 bps INFO @ Tue, 02 Aug 2022 13:25:29: #1 tag size = 74 INFO @ Tue, 02 Aug 2022 13:25:29: #1 total tags in treatment: 15716936 INFO @ Tue, 02 Aug 2022 13:25:29: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 13:25:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 13:25:29: #1 tags after filtering in treatment: 15716936 INFO @ Tue, 02 Aug 2022 13:25:29: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 13:25:29: #1 finished! INFO @ Tue, 02 Aug 2022 13:25:29: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 13:25:29: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 13:25:30: #2 number of paired peaks: 95 WARNING @ Tue, 02 Aug 2022 13:25:30: Too few paired peaks (95) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 13:25:30: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX12261327/SRX12261327.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX12261327/SRX12261327.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX12261327/SRX12261327.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX12261327/SRX12261327.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 13:25:31: 8000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 02 Aug 2022 13:25:37: 12000000 INFO @ Tue, 02 Aug 2022 13:25:39: 9000000 INFO @ Tue, 02 Aug 2022 13:25:47: 13000000 INFO @ Tue, 02 Aug 2022 13:25:48: 10000000 INFO @ Tue, 02 Aug 2022 13:25:56: 14000000 INFO @ Tue, 02 Aug 2022 13:25:57: 11000000 INFO @ Tue, 02 Aug 2022 13:26:05: 15000000 INFO @ Tue, 02 Aug 2022 13:26:06: 12000000 BigWig に変換しました。 INFO @ Tue, 02 Aug 2022 13:26:11: #1 tag size is determined as 74 bps INFO @ Tue, 02 Aug 2022 13:26:11: #1 tag size = 74 INFO @ Tue, 02 Aug 2022 13:26:11: #1 total tags in treatment: 15716936 INFO @ Tue, 02 Aug 2022 13:26:11: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 13:26:11: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 13:26:11: #1 tags after filtering in treatment: 15716936 INFO @ Tue, 02 Aug 2022 13:26:11: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 13:26:11: #1 finished! INFO @ Tue, 02 Aug 2022 13:26:11: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 13:26:11: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 13:26:12: #2 number of paired peaks: 95 WARNING @ Tue, 02 Aug 2022 13:26:12: Too few paired peaks (95) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 13:26:12: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX12261327/SRX12261327.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX12261327/SRX12261327.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX12261327/SRX12261327.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX12261327/SRX12261327.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 13:26:15: 13000000 INFO @ Tue, 02 Aug 2022 13:26:23: 14000000 INFO @ Tue, 02 Aug 2022 13:26:31: 15000000 INFO @ Tue, 02 Aug 2022 13:26:36: #1 tag size is determined as 74 bps INFO @ Tue, 02 Aug 2022 13:26:36: #1 tag size = 74 INFO @ Tue, 02 Aug 2022 13:26:36: #1 total tags in treatment: 15716936 INFO @ Tue, 02 Aug 2022 13:26:36: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 13:26:36: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 13:26:36: #1 tags after filtering in treatment: 15716936 INFO @ Tue, 02 Aug 2022 13:26:36: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 13:26:36: #1 finished! INFO @ Tue, 02 Aug 2022 13:26:36: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 13:26:36: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 13:26:37: #2 number of paired peaks: 95 WARNING @ Tue, 02 Aug 2022 13:26:37: Too few paired peaks (95) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 13:26:37: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX12261327/SRX12261327.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX12261327/SRX12261327.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX12261327/SRX12261327.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX12261327/SRX12261327.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling