Job ID = 16436212 SRX = SRX12178860 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2022-08-02T02:06:13 prefetch.2.10.7: 1) Downloading 'SRR15888054'... 2022-08-02T02:06:13 prefetch.2.10.7: Downloading via HTTPS... 2022-08-02T02:08:33 prefetch.2.10.7: HTTPS download succeed 2022-08-02T02:08:33 prefetch.2.10.7: 1) 'SRR15888054' was downloaded successfully 2022-08-02T02:08:33 prefetch.2.10.7: 'SRR15888054' has 0 unresolved dependencies Read 42044911 spots for SRR15888054/SRR15888054.sra Written 42044911 spots for SRR15888054/SRR15888054.sra fastq に変換しました。 bowtie でマッピング中... Your job 16436827 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:01 Multiseed full-index search: 00:37:34 42044911 reads; of these: 42044911 (100.00%) were paired; of these: 13937980 (33.15%) aligned concordantly 0 times 22559363 (53.66%) aligned concordantly exactly 1 time 5547568 (13.19%) aligned concordantly >1 times ---- 13937980 pairs aligned concordantly 0 times; of these: 4207473 (30.19%) aligned discordantly 1 time ---- 9730507 pairs aligned 0 times concordantly or discordantly; of these: 19461014 mates make up the pairs; of these: 14866450 (76.39%) aligned 0 times 3061426 (15.73%) aligned exactly 1 time 1533138 (7.88%) aligned >1 times 82.32% overall alignment rate Time searching: 00:37:35 Overall time: 00:37:35 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 32 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 8638751 / 32136288 = 0.2688 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 12:05:24: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX12178860/SRX12178860.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX12178860/SRX12178860.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX12178860/SRX12178860.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX12178860/SRX12178860.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 12:05:24: #1 read tag files... INFO @ Tue, 02 Aug 2022 12:05:24: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 12:05:30: 1000000 INFO @ Tue, 02 Aug 2022 12:05:36: 2000000 INFO @ Tue, 02 Aug 2022 12:05:42: 3000000 INFO @ Tue, 02 Aug 2022 12:05:48: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 12:05:53: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX12178860/SRX12178860.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX12178860/SRX12178860.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX12178860/SRX12178860.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX12178860/SRX12178860.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 12:05:53: #1 read tag files... INFO @ Tue, 02 Aug 2022 12:05:53: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 12:05:54: 5000000 INFO @ Tue, 02 Aug 2022 12:06:01: 1000000 INFO @ Tue, 02 Aug 2022 12:06:01: 6000000 INFO @ Tue, 02 Aug 2022 12:06:08: 2000000 INFO @ Tue, 02 Aug 2022 12:06:09: 7000000 INFO @ Tue, 02 Aug 2022 12:06:15: 3000000 INFO @ Tue, 02 Aug 2022 12:06:16: 8000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 12:06:22: 4000000 INFO @ Tue, 02 Aug 2022 12:06:23: 9000000 INFO @ Tue, 02 Aug 2022 12:06:23: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX12178860/SRX12178860.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX12178860/SRX12178860.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX12178860/SRX12178860.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX12178860/SRX12178860.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 12:06:23: #1 read tag files... INFO @ Tue, 02 Aug 2022 12:06:23: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 12:06:30: 5000000 INFO @ Tue, 02 Aug 2022 12:06:30: 10000000 INFO @ Tue, 02 Aug 2022 12:06:30: 1000000 INFO @ Tue, 02 Aug 2022 12:06:37: 6000000 INFO @ Tue, 02 Aug 2022 12:06:38: 11000000 INFO @ Tue, 02 Aug 2022 12:06:38: 2000000 INFO @ Tue, 02 Aug 2022 12:06:44: 7000000 INFO @ Tue, 02 Aug 2022 12:06:45: 3000000 INFO @ Tue, 02 Aug 2022 12:06:45: 12000000 INFO @ Tue, 02 Aug 2022 12:06:51: 8000000 INFO @ Tue, 02 Aug 2022 12:06:52: 4000000 INFO @ Tue, 02 Aug 2022 12:06:52: 13000000 INFO @ Tue, 02 Aug 2022 12:06:59: 9000000 INFO @ Tue, 02 Aug 2022 12:06:59: 5000000 INFO @ Tue, 02 Aug 2022 12:07:00: 14000000 INFO @ Tue, 02 Aug 2022 12:07:06: 10000000 INFO @ Tue, 02 Aug 2022 12:07:06: 6000000 INFO @ Tue, 02 Aug 2022 12:07:07: 15000000 INFO @ Tue, 02 Aug 2022 12:07:13: 11000000 INFO @ Tue, 02 Aug 2022 12:07:13: 7000000 INFO @ Tue, 02 Aug 2022 12:07:14: 16000000 INFO @ Tue, 02 Aug 2022 12:07:20: 12000000 INFO @ Tue, 02 Aug 2022 12:07:20: 8000000 INFO @ Tue, 02 Aug 2022 12:07:21: 17000000 INFO @ Tue, 02 Aug 2022 12:07:27: 9000000 INFO @ Tue, 02 Aug 2022 12:07:28: 13000000 INFO @ Tue, 02 Aug 2022 12:07:28: 18000000 INFO @ Tue, 02 Aug 2022 12:07:34: 10000000 INFO @ Tue, 02 Aug 2022 12:07:35: 14000000 INFO @ Tue, 02 Aug 2022 12:07:35: 19000000 INFO @ Tue, 02 Aug 2022 12:07:41: 11000000 INFO @ Tue, 02 Aug 2022 12:07:42: 20000000 INFO @ Tue, 02 Aug 2022 12:07:42: 15000000 INFO @ Tue, 02 Aug 2022 12:07:48: 12000000 INFO @ Tue, 02 Aug 2022 12:07:49: 21000000 INFO @ Tue, 02 Aug 2022 12:07:49: 16000000 INFO @ Tue, 02 Aug 2022 12:07:55: 13000000 INFO @ Tue, 02 Aug 2022 12:07:56: 22000000 INFO @ Tue, 02 Aug 2022 12:07:56: 17000000 INFO @ Tue, 02 Aug 2022 12:08:02: 14000000 INFO @ Tue, 02 Aug 2022 12:08:03: 23000000 INFO @ Tue, 02 Aug 2022 12:08:03: 18000000 INFO @ Tue, 02 Aug 2022 12:08:09: 15000000 INFO @ Tue, 02 Aug 2022 12:08:09: 24000000 INFO @ Tue, 02 Aug 2022 12:08:10: 19000000 INFO @ Tue, 02 Aug 2022 12:08:15: 16000000 INFO @ Tue, 02 Aug 2022 12:08:16: 25000000 INFO @ Tue, 02 Aug 2022 12:08:17: 20000000 INFO @ Tue, 02 Aug 2022 12:08:22: 17000000 INFO @ Tue, 02 Aug 2022 12:08:23: 26000000 INFO @ Tue, 02 Aug 2022 12:08:24: 21000000 INFO @ Tue, 02 Aug 2022 12:08:29: 18000000 INFO @ Tue, 02 Aug 2022 12:08:30: 27000000 INFO @ Tue, 02 Aug 2022 12:08:31: 22000000 INFO @ Tue, 02 Aug 2022 12:08:36: 19000000 INFO @ Tue, 02 Aug 2022 12:08:36: 28000000 INFO @ Tue, 02 Aug 2022 12:08:38: 23000000 INFO @ Tue, 02 Aug 2022 12:08:43: 20000000 INFO @ Tue, 02 Aug 2022 12:08:43: 29000000 INFO @ Tue, 02 Aug 2022 12:08:45: 24000000 INFO @ Tue, 02 Aug 2022 12:08:50: 21000000 INFO @ Tue, 02 Aug 2022 12:08:50: 30000000 INFO @ Tue, 02 Aug 2022 12:08:52: 25000000 INFO @ Tue, 02 Aug 2022 12:08:57: 22000000 INFO @ Tue, 02 Aug 2022 12:08:57: 31000000 INFO @ Tue, 02 Aug 2022 12:08:59: 26000000 INFO @ Tue, 02 Aug 2022 12:09:03: 23000000 INFO @ Tue, 02 Aug 2022 12:09:04: 32000000 INFO @ Tue, 02 Aug 2022 12:09:06: 27000000 INFO @ Tue, 02 Aug 2022 12:09:10: 24000000 INFO @ Tue, 02 Aug 2022 12:09:11: 33000000 INFO @ Tue, 02 Aug 2022 12:09:13: 28000000 INFO @ Tue, 02 Aug 2022 12:09:17: 25000000 INFO @ Tue, 02 Aug 2022 12:09:17: 34000000 INFO @ Tue, 02 Aug 2022 12:09:20: 29000000 INFO @ Tue, 02 Aug 2022 12:09:24: 26000000 INFO @ Tue, 02 Aug 2022 12:09:24: 35000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 02 Aug 2022 12:09:28: 30000000 INFO @ Tue, 02 Aug 2022 12:09:31: 27000000 INFO @ Tue, 02 Aug 2022 12:09:31: 36000000 INFO @ Tue, 02 Aug 2022 12:09:35: 31000000 INFO @ Tue, 02 Aug 2022 12:09:37: 28000000 INFO @ Tue, 02 Aug 2022 12:09:38: 37000000 INFO @ Tue, 02 Aug 2022 12:09:42: 32000000 INFO @ Tue, 02 Aug 2022 12:09:44: 29000000 INFO @ Tue, 02 Aug 2022 12:09:45: 38000000 INFO @ Tue, 02 Aug 2022 12:09:49: 33000000 INFO @ Tue, 02 Aug 2022 12:09:51: 30000000 INFO @ Tue, 02 Aug 2022 12:09:51: 39000000 INFO @ Tue, 02 Aug 2022 12:09:57: 34000000 INFO @ Tue, 02 Aug 2022 12:09:58: 31000000 INFO @ Tue, 02 Aug 2022 12:09:58: 40000000 INFO @ Tue, 02 Aug 2022 12:10:04: 35000000 INFO @ Tue, 02 Aug 2022 12:10:05: 32000000 INFO @ Tue, 02 Aug 2022 12:10:05: 41000000 INFO @ Tue, 02 Aug 2022 12:10:11: 36000000 INFO @ Tue, 02 Aug 2022 12:10:11: 33000000 INFO @ Tue, 02 Aug 2022 12:10:12: 42000000 INFO @ Tue, 02 Aug 2022 12:10:18: 37000000 INFO @ Tue, 02 Aug 2022 12:10:18: 34000000 INFO @ Tue, 02 Aug 2022 12:10:19: 43000000 INFO @ Tue, 02 Aug 2022 12:10:25: 38000000 INFO @ Tue, 02 Aug 2022 12:10:25: 35000000 INFO @ Tue, 02 Aug 2022 12:10:26: 44000000 INFO @ Tue, 02 Aug 2022 12:10:32: 39000000 INFO @ Tue, 02 Aug 2022 12:10:32: 36000000 INFO @ Tue, 02 Aug 2022 12:10:32: 45000000 BigWig に変換しました。 INFO @ Tue, 02 Aug 2022 12:10:39: 46000000 INFO @ Tue, 02 Aug 2022 12:10:39: 37000000 INFO @ Tue, 02 Aug 2022 12:10:39: 40000000 INFO @ Tue, 02 Aug 2022 12:10:46: 47000000 INFO @ Tue, 02 Aug 2022 12:10:46: 38000000 INFO @ Tue, 02 Aug 2022 12:10:46: 41000000 INFO @ Tue, 02 Aug 2022 12:10:52: 48000000 INFO @ Tue, 02 Aug 2022 12:10:53: 39000000 INFO @ Tue, 02 Aug 2022 12:10:54: 42000000 INFO @ Tue, 02 Aug 2022 12:10:59: 49000000 INFO @ Tue, 02 Aug 2022 12:11:00: 40000000 INFO @ Tue, 02 Aug 2022 12:11:01: 43000000 INFO @ Tue, 02 Aug 2022 12:11:06: 50000000 INFO @ Tue, 02 Aug 2022 12:11:07: 41000000 INFO @ Tue, 02 Aug 2022 12:11:09: 44000000 INFO @ Tue, 02 Aug 2022 12:11:13: 51000000 INFO @ Tue, 02 Aug 2022 12:11:14: 42000000 INFO @ Tue, 02 Aug 2022 12:11:16: 45000000 INFO @ Tue, 02 Aug 2022 12:11:19: #1 tag size is determined as 50 bps INFO @ Tue, 02 Aug 2022 12:11:19: #1 tag size = 50 INFO @ Tue, 02 Aug 2022 12:11:19: #1 total tags in treatment: 20278167 INFO @ Tue, 02 Aug 2022 12:11:19: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 12:11:19: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 12:11:19: #1 tags after filtering in treatment: 14760250 INFO @ Tue, 02 Aug 2022 12:11:19: #1 Redundant rate of treatment: 0.27 INFO @ Tue, 02 Aug 2022 12:11:19: #1 finished! INFO @ Tue, 02 Aug 2022 12:11:19: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 12:11:19: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 12:11:20: #2 number of paired peaks: 402 WARNING @ Tue, 02 Aug 2022 12:11:20: Fewer paired peaks (402) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 402 pairs to build model! INFO @ Tue, 02 Aug 2022 12:11:20: start model_add_line... INFO @ Tue, 02 Aug 2022 12:11:21: start X-correlation... INFO @ Tue, 02 Aug 2022 12:11:21: end of X-cor INFO @ Tue, 02 Aug 2022 12:11:21: #2 finished! INFO @ Tue, 02 Aug 2022 12:11:21: #2 predicted fragment length is 46 bps INFO @ Tue, 02 Aug 2022 12:11:21: #2 alternative fragment length(s) may be 4,46 bps INFO @ Tue, 02 Aug 2022 12:11:21: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX12178860/SRX12178860.05_model.r WARNING @ Tue, 02 Aug 2022 12:11:21: #2 Since the d (46) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 02 Aug 2022 12:11:21: #2 You may need to consider one of the other alternative d(s): 4,46 WARNING @ Tue, 02 Aug 2022 12:11:21: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 02 Aug 2022 12:11:21: #3 Call peaks... INFO @ Tue, 02 Aug 2022 12:11:21: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 02 Aug 2022 12:11:21: 43000000 INFO @ Tue, 02 Aug 2022 12:11:23: 46000000 INFO @ Tue, 02 Aug 2022 12:11:28: 44000000 INFO @ Tue, 02 Aug 2022 12:11:30: 47000000 INFO @ Tue, 02 Aug 2022 12:11:34: 45000000 INFO @ Tue, 02 Aug 2022 12:11:37: 48000000 INFO @ Tue, 02 Aug 2022 12:11:41: 46000000 INFO @ Tue, 02 Aug 2022 12:11:44: 49000000 INFO @ Tue, 02 Aug 2022 12:11:47: 47000000 INFO @ Tue, 02 Aug 2022 12:11:49: #3 Call peaks for each chromosome... INFO @ Tue, 02 Aug 2022 12:11:50: 50000000 INFO @ Tue, 02 Aug 2022 12:11:53: 48000000 INFO @ Tue, 02 Aug 2022 12:11:57: 51000000 INFO @ Tue, 02 Aug 2022 12:12:00: 49000000 INFO @ Tue, 02 Aug 2022 12:12:04: #1 tag size is determined as 50 bps INFO @ Tue, 02 Aug 2022 12:12:04: #1 tag size = 50 INFO @ Tue, 02 Aug 2022 12:12:04: #1 total tags in treatment: 20278167 INFO @ Tue, 02 Aug 2022 12:12:04: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 12:12:04: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 12:12:04: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX12178860/SRX12178860.05_peaks.xls INFO @ Tue, 02 Aug 2022 12:12:04: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX12178860/SRX12178860.05_peaks.narrowPeak INFO @ Tue, 02 Aug 2022 12:12:04: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX12178860/SRX12178860.05_summits.bed INFO @ Tue, 02 Aug 2022 12:12:04: #1 tags after filtering in treatment: 14760250 INFO @ Tue, 02 Aug 2022 12:12:04: #1 Redundant rate of treatment: 0.27 INFO @ Tue, 02 Aug 2022 12:12:04: #1 finished! INFO @ Tue, 02 Aug 2022 12:12:04: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 12:12:04: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 12:12:04: Done! pass1 - making usageList (15 chroms): 3 millis pass2 - checking and writing primary data (14947 records, 4 fields): 56 millis CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 12:12:05: #2 number of paired peaks: 402 WARNING @ Tue, 02 Aug 2022 12:12:05: Fewer paired peaks (402) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 402 pairs to build model! INFO @ Tue, 02 Aug 2022 12:12:05: start model_add_line... INFO @ Tue, 02 Aug 2022 12:12:05: start X-correlation... INFO @ Tue, 02 Aug 2022 12:12:05: end of X-cor INFO @ Tue, 02 Aug 2022 12:12:05: #2 finished! INFO @ Tue, 02 Aug 2022 12:12:05: #2 predicted fragment length is 46 bps INFO @ Tue, 02 Aug 2022 12:12:05: #2 alternative fragment length(s) may be 4,46 bps INFO @ Tue, 02 Aug 2022 12:12:05: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX12178860/SRX12178860.10_model.r WARNING @ Tue, 02 Aug 2022 12:12:05: #2 Since the d (46) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 02 Aug 2022 12:12:05: #2 You may need to consider one of the other alternative d(s): 4,46 WARNING @ Tue, 02 Aug 2022 12:12:05: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 02 Aug 2022 12:12:05: #3 Call peaks... INFO @ Tue, 02 Aug 2022 12:12:05: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 02 Aug 2022 12:12:06: 50000000 INFO @ Tue, 02 Aug 2022 12:12:12: 51000000 INFO @ Tue, 02 Aug 2022 12:12:17: #1 tag size is determined as 50 bps INFO @ Tue, 02 Aug 2022 12:12:17: #1 tag size = 50 INFO @ Tue, 02 Aug 2022 12:12:17: #1 total tags in treatment: 20278167 INFO @ Tue, 02 Aug 2022 12:12:17: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 12:12:17: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 12:12:17: #1 tags after filtering in treatment: 14760250 INFO @ Tue, 02 Aug 2022 12:12:17: #1 Redundant rate of treatment: 0.27 INFO @ Tue, 02 Aug 2022 12:12:17: #1 finished! INFO @ Tue, 02 Aug 2022 12:12:17: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 12:12:17: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 12:12:18: #2 number of paired peaks: 402 WARNING @ Tue, 02 Aug 2022 12:12:18: Fewer paired peaks (402) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 402 pairs to build model! INFO @ Tue, 02 Aug 2022 12:12:18: start model_add_line... INFO @ Tue, 02 Aug 2022 12:12:18: start X-correlation... INFO @ Tue, 02 Aug 2022 12:12:18: end of X-cor INFO @ Tue, 02 Aug 2022 12:12:18: #2 finished! INFO @ Tue, 02 Aug 2022 12:12:18: #2 predicted fragment length is 46 bps INFO @ Tue, 02 Aug 2022 12:12:18: #2 alternative fragment length(s) may be 4,46 bps INFO @ Tue, 02 Aug 2022 12:12:18: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX12178860/SRX12178860.20_model.r WARNING @ Tue, 02 Aug 2022 12:12:18: #2 Since the d (46) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 02 Aug 2022 12:12:18: #2 You may need to consider one of the other alternative d(s): 4,46 WARNING @ Tue, 02 Aug 2022 12:12:18: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 02 Aug 2022 12:12:18: #3 Call peaks... INFO @ Tue, 02 Aug 2022 12:12:18: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 02 Aug 2022 12:12:33: #3 Call peaks for each chromosome... INFO @ Tue, 02 Aug 2022 12:12:45: #3 Call peaks for each chromosome... INFO @ Tue, 02 Aug 2022 12:12:48: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX12178860/SRX12178860.10_peaks.xls INFO @ Tue, 02 Aug 2022 12:12:48: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX12178860/SRX12178860.10_peaks.narrowPeak INFO @ Tue, 02 Aug 2022 12:12:48: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX12178860/SRX12178860.10_summits.bed INFO @ Tue, 02 Aug 2022 12:12:49: Done! pass1 - making usageList (14 chroms): 1 millis pass2 - checking and writing primary data (5448 records, 4 fields): 35 millis CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 12:13:01: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX12178860/SRX12178860.20_peaks.xls INFO @ Tue, 02 Aug 2022 12:13:01: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX12178860/SRX12178860.20_peaks.narrowPeak INFO @ Tue, 02 Aug 2022 12:13:01: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX12178860/SRX12178860.20_summits.bed INFO @ Tue, 02 Aug 2022 12:13:01: Done! pass1 - making usageList (10 chroms): 1 millis pass2 - checking and writing primary data (248 records, 4 fields): 37 millis CompletedMACS2peakCalling