Job ID = 16440260 SRX = SRX12178841 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2022-08-02T08:38:55 prefetch.2.10.7: 1) Downloading 'SRR15888073'... 2022-08-02T08:38:55 prefetch.2.10.7: Downloading via HTTPS... 2022-08-02T08:41:15 prefetch.2.10.7: HTTPS download succeed 2022-08-02T08:41:15 prefetch.2.10.7: 1) 'SRR15888073' was downloaded successfully 2022-08-02T08:41:15 prefetch.2.10.7: 'SRR15888073' has 0 unresolved dependencies Read 40466657 spots for SRR15888073/SRR15888073.sra Written 40466657 spots for SRR15888073/SRR15888073.sra fastq に変換しました。 bowtie でマッピング中... Your job 16441205 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 01:08:03 40466657 reads; of these: 40466657 (100.00%) were paired; of these: 13954946 (34.49%) aligned concordantly 0 times 22442098 (55.46%) aligned concordantly exactly 1 time 4069613 (10.06%) aligned concordantly >1 times ---- 13954946 pairs aligned concordantly 0 times; of these: 5589128 (40.05%) aligned discordantly 1 time ---- 8365818 pairs aligned 0 times concordantly or discordantly; of these: 16731636 mates make up the pairs; of these: 9795168 (58.54%) aligned 0 times 5021221 (30.01%) aligned exactly 1 time 1915247 (11.45%) aligned >1 times 87.90% overall alignment rate Time searching: 01:08:03 Overall time: 01:08:03 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 32 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 4917489 / 32009954 = 0.1536 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 19:21:28: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX12178841/SRX12178841.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX12178841/SRX12178841.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX12178841/SRX12178841.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX12178841/SRX12178841.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 19:21:28: #1 read tag files... INFO @ Tue, 02 Aug 2022 19:21:28: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 19:21:39: 1000000 INFO @ Tue, 02 Aug 2022 19:21:50: 2000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 19:21:56: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX12178841/SRX12178841.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX12178841/SRX12178841.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX12178841/SRX12178841.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX12178841/SRX12178841.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 19:21:56: #1 read tag files... INFO @ Tue, 02 Aug 2022 19:21:56: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 19:22:01: 3000000 INFO @ Tue, 02 Aug 2022 19:22:06: 1000000 INFO @ Tue, 02 Aug 2022 19:22:12: 4000000 INFO @ Tue, 02 Aug 2022 19:22:16: 2000000 INFO @ Tue, 02 Aug 2022 19:22:23: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 19:22:26: 3000000 INFO @ Tue, 02 Aug 2022 19:22:26: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX12178841/SRX12178841.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX12178841/SRX12178841.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX12178841/SRX12178841.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX12178841/SRX12178841.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 19:22:26: #1 read tag files... INFO @ Tue, 02 Aug 2022 19:22:26: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 19:22:34: 6000000 INFO @ Tue, 02 Aug 2022 19:22:36: 4000000 INFO @ Tue, 02 Aug 2022 19:22:38: 1000000 INFO @ Tue, 02 Aug 2022 19:22:46: 5000000 INFO @ Tue, 02 Aug 2022 19:22:46: 7000000 INFO @ Tue, 02 Aug 2022 19:22:49: 2000000 INFO @ Tue, 02 Aug 2022 19:22:55: 6000000 INFO @ Tue, 02 Aug 2022 19:22:58: 8000000 INFO @ Tue, 02 Aug 2022 19:23:02: 3000000 INFO @ Tue, 02 Aug 2022 19:23:05: 7000000 INFO @ Tue, 02 Aug 2022 19:23:10: 9000000 INFO @ Tue, 02 Aug 2022 19:23:13: 4000000 INFO @ Tue, 02 Aug 2022 19:23:15: 8000000 INFO @ Tue, 02 Aug 2022 19:23:22: 10000000 INFO @ Tue, 02 Aug 2022 19:23:25: 9000000 INFO @ Tue, 02 Aug 2022 19:23:25: 5000000 INFO @ Tue, 02 Aug 2022 19:23:33: 11000000 INFO @ Tue, 02 Aug 2022 19:23:35: 10000000 INFO @ Tue, 02 Aug 2022 19:23:37: 6000000 INFO @ Tue, 02 Aug 2022 19:23:45: 11000000 INFO @ Tue, 02 Aug 2022 19:23:45: 12000000 INFO @ Tue, 02 Aug 2022 19:23:49: 7000000 INFO @ Tue, 02 Aug 2022 19:23:54: 12000000 INFO @ Tue, 02 Aug 2022 19:23:57: 13000000 INFO @ Tue, 02 Aug 2022 19:24:01: 8000000 INFO @ Tue, 02 Aug 2022 19:24:04: 13000000 INFO @ Tue, 02 Aug 2022 19:24:09: 14000000 INFO @ Tue, 02 Aug 2022 19:24:13: 9000000 INFO @ Tue, 02 Aug 2022 19:24:14: 14000000 INFO @ Tue, 02 Aug 2022 19:24:21: 15000000 INFO @ Tue, 02 Aug 2022 19:24:24: 15000000 INFO @ Tue, 02 Aug 2022 19:24:25: 10000000 INFO @ Tue, 02 Aug 2022 19:24:33: 16000000 INFO @ Tue, 02 Aug 2022 19:24:33: 16000000 INFO @ Tue, 02 Aug 2022 19:24:37: 11000000 INFO @ Tue, 02 Aug 2022 19:24:43: 17000000 INFO @ Tue, 02 Aug 2022 19:24:45: 17000000 INFO @ Tue, 02 Aug 2022 19:24:49: 12000000 INFO @ Tue, 02 Aug 2022 19:24:53: 18000000 INFO @ Tue, 02 Aug 2022 19:24:57: 18000000 INFO @ Tue, 02 Aug 2022 19:25:01: 13000000 INFO @ Tue, 02 Aug 2022 19:25:03: 19000000 INFO @ Tue, 02 Aug 2022 19:25:09: 19000000 INFO @ Tue, 02 Aug 2022 19:25:12: 20000000 INFO @ Tue, 02 Aug 2022 19:25:13: 14000000 INFO @ Tue, 02 Aug 2022 19:25:21: 20000000 INFO @ Tue, 02 Aug 2022 19:25:22: 21000000 INFO @ Tue, 02 Aug 2022 19:25:25: 15000000 INFO @ Tue, 02 Aug 2022 19:25:32: 22000000 INFO @ Tue, 02 Aug 2022 19:25:33: 21000000 INFO @ Tue, 02 Aug 2022 19:25:37: 16000000 INFO @ Tue, 02 Aug 2022 19:25:42: 23000000 INFO @ Tue, 02 Aug 2022 19:25:44: 22000000 INFO @ Tue, 02 Aug 2022 19:25:48: 17000000 INFO @ Tue, 02 Aug 2022 19:25:52: 24000000 INFO @ Tue, 02 Aug 2022 19:25:56: 23000000 INFO @ Tue, 02 Aug 2022 19:26:00: 18000000 INFO @ Tue, 02 Aug 2022 19:26:02: 25000000 INFO @ Tue, 02 Aug 2022 19:26:08: 24000000 INFO @ Tue, 02 Aug 2022 19:26:12: 19000000 INFO @ Tue, 02 Aug 2022 19:26:12: 26000000 INFO @ Tue, 02 Aug 2022 19:26:20: 25000000 INFO @ Tue, 02 Aug 2022 19:26:22: 27000000 INFO @ Tue, 02 Aug 2022 19:26:24: 20000000 INFO @ Tue, 02 Aug 2022 19:26:32: 26000000 INFO @ Tue, 02 Aug 2022 19:26:32: 28000000 INFO @ Tue, 02 Aug 2022 19:26:36: 21000000 INFO @ Tue, 02 Aug 2022 19:26:42: 29000000 INFO @ Tue, 02 Aug 2022 19:26:44: 27000000 INFO @ Tue, 02 Aug 2022 19:26:47: 22000000 INFO @ Tue, 02 Aug 2022 19:26:52: 30000000 INFO @ Tue, 02 Aug 2022 19:26:56: 28000000 INFO @ Tue, 02 Aug 2022 19:26:59: 23000000 INFO @ Tue, 02 Aug 2022 19:27:02: 31000000 INFO @ Tue, 02 Aug 2022 19:27:08: 29000000 INFO @ Tue, 02 Aug 2022 19:27:11: 24000000 INFO @ Tue, 02 Aug 2022 19:27:11: 32000000 INFO @ Tue, 02 Aug 2022 19:27:20: 30000000 INFO @ Tue, 02 Aug 2022 19:27:21: 33000000 INFO @ Tue, 02 Aug 2022 19:27:22: 25000000 INFO @ Tue, 02 Aug 2022 19:27:31: 31000000 INFO @ Tue, 02 Aug 2022 19:27:31: 34000000 INFO @ Tue, 02 Aug 2022 19:27:34: 26000000 INFO @ Tue, 02 Aug 2022 19:27:41: 35000000 INFO @ Tue, 02 Aug 2022 19:27:42: 32000000 INFO @ Tue, 02 Aug 2022 19:27:45: 27000000 INFO @ Tue, 02 Aug 2022 19:27:50: 36000000 INFO @ Tue, 02 Aug 2022 19:27:53: 33000000 INFO @ Tue, 02 Aug 2022 19:27:56: 28000000 INFO @ Tue, 02 Aug 2022 19:28:00: 37000000 INFO @ Tue, 02 Aug 2022 19:28:04: 34000000 INFO @ Tue, 02 Aug 2022 19:28:07: 29000000 INFO @ Tue, 02 Aug 2022 19:28:09: 38000000 INFO @ Tue, 02 Aug 2022 19:28:15: 35000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 02 Aug 2022 19:28:19: 30000000 INFO @ Tue, 02 Aug 2022 19:28:19: 39000000 INFO @ Tue, 02 Aug 2022 19:28:26: 36000000 INFO @ Tue, 02 Aug 2022 19:28:28: 40000000 INFO @ Tue, 02 Aug 2022 19:28:30: 31000000 INFO @ Tue, 02 Aug 2022 19:28:37: 37000000 INFO @ Tue, 02 Aug 2022 19:28:37: 41000000 INFO @ Tue, 02 Aug 2022 19:28:41: 32000000 INFO @ Tue, 02 Aug 2022 19:28:47: 42000000 INFO @ Tue, 02 Aug 2022 19:28:48: 38000000 INFO @ Tue, 02 Aug 2022 19:28:52: 33000000 INFO @ Tue, 02 Aug 2022 19:28:56: 43000000 INFO @ Tue, 02 Aug 2022 19:28:59: 39000000 INFO @ Tue, 02 Aug 2022 19:29:01: 34000000 INFO @ Tue, 02 Aug 2022 19:29:06: 44000000 INFO @ Tue, 02 Aug 2022 19:29:09: 40000000 INFO @ Tue, 02 Aug 2022 19:29:10: 35000000 INFO @ Tue, 02 Aug 2022 19:29:15: 45000000 INFO @ Tue, 02 Aug 2022 19:29:19: 41000000 INFO @ Tue, 02 Aug 2022 19:29:19: 36000000 INFO @ Tue, 02 Aug 2022 19:29:25: 46000000 INFO @ Tue, 02 Aug 2022 19:29:28: 37000000 INFO @ Tue, 02 Aug 2022 19:29:29: 42000000 INFO @ Tue, 02 Aug 2022 19:29:34: 47000000 INFO @ Tue, 02 Aug 2022 19:29:37: 38000000 INFO @ Tue, 02 Aug 2022 19:29:39: 43000000 INFO @ Tue, 02 Aug 2022 19:29:43: 48000000 INFO @ Tue, 02 Aug 2022 19:29:46: 39000000 INFO @ Tue, 02 Aug 2022 19:29:49: 44000000 INFO @ Tue, 02 Aug 2022 19:29:53: 49000000 INFO @ Tue, 02 Aug 2022 19:29:56: 40000000 INFO @ Tue, 02 Aug 2022 19:30:00: 45000000 INFO @ Tue, 02 Aug 2022 19:30:02: 50000000 BigWig に変換しました。 INFO @ Tue, 02 Aug 2022 19:30:05: 41000000 INFO @ Tue, 02 Aug 2022 19:30:11: 46000000 INFO @ Tue, 02 Aug 2022 19:30:12: 51000000 INFO @ Tue, 02 Aug 2022 19:30:15: 42000000 INFO @ Tue, 02 Aug 2022 19:30:21: 47000000 INFO @ Tue, 02 Aug 2022 19:30:22: 52000000 INFO @ Tue, 02 Aug 2022 19:30:25: 43000000 INFO @ Tue, 02 Aug 2022 19:30:31: 53000000 INFO @ Tue, 02 Aug 2022 19:30:31: 48000000 INFO @ Tue, 02 Aug 2022 19:30:34: 44000000 INFO @ Tue, 02 Aug 2022 19:30:41: 54000000 INFO @ Tue, 02 Aug 2022 19:30:42: 49000000 INFO @ Tue, 02 Aug 2022 19:30:44: 45000000 INFO @ Tue, 02 Aug 2022 19:30:50: 55000000 INFO @ Tue, 02 Aug 2022 19:30:52: 50000000 INFO @ Tue, 02 Aug 2022 19:30:55: 46000000 INFO @ Tue, 02 Aug 2022 19:31:00: 56000000 INFO @ Tue, 02 Aug 2022 19:31:02: 51000000 INFO @ Tue, 02 Aug 2022 19:31:04: 47000000 INFO @ Tue, 02 Aug 2022 19:31:09: 57000000 INFO @ Tue, 02 Aug 2022 19:31:12: 52000000 INFO @ Tue, 02 Aug 2022 19:31:13: 48000000 INFO @ Tue, 02 Aug 2022 19:31:19: 58000000 INFO @ Tue, 02 Aug 2022 19:31:23: 53000000 INFO @ Tue, 02 Aug 2022 19:31:23: 49000000 INFO @ Tue, 02 Aug 2022 19:31:29: 59000000 INFO @ Tue, 02 Aug 2022 19:31:32: 50000000 INFO @ Tue, 02 Aug 2022 19:31:33: 54000000 INFO @ Tue, 02 Aug 2022 19:31:39: 60000000 INFO @ Tue, 02 Aug 2022 19:31:42: 51000000 INFO @ Tue, 02 Aug 2022 19:31:43: 55000000 INFO @ Tue, 02 Aug 2022 19:31:49: 61000000 INFO @ Tue, 02 Aug 2022 19:31:52: #1 tag size is determined as 50 bps INFO @ Tue, 02 Aug 2022 19:31:52: #1 tag size = 50 INFO @ Tue, 02 Aug 2022 19:31:52: #1 total tags in treatment: 22170832 INFO @ Tue, 02 Aug 2022 19:31:52: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 19:31:52: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 19:31:53: 56000000 INFO @ Tue, 02 Aug 2022 19:31:53: 52000000 INFO @ Tue, 02 Aug 2022 19:31:53: #1 tags after filtering in treatment: 14956079 INFO @ Tue, 02 Aug 2022 19:31:53: #1 Redundant rate of treatment: 0.33 INFO @ Tue, 02 Aug 2022 19:31:53: #1 finished! INFO @ Tue, 02 Aug 2022 19:31:53: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 19:31:53: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 19:31:55: #2 number of paired peaks: 850 WARNING @ Tue, 02 Aug 2022 19:31:55: Fewer paired peaks (850) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 850 pairs to build model! INFO @ Tue, 02 Aug 2022 19:31:55: start model_add_line... INFO @ Tue, 02 Aug 2022 19:31:55: start X-correlation... INFO @ Tue, 02 Aug 2022 19:31:55: end of X-cor INFO @ Tue, 02 Aug 2022 19:31:55: #2 finished! INFO @ Tue, 02 Aug 2022 19:31:55: #2 predicted fragment length is 69 bps INFO @ Tue, 02 Aug 2022 19:31:55: #2 alternative fragment length(s) may be 4,69 bps INFO @ Tue, 02 Aug 2022 19:31:55: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX12178841/SRX12178841.10_model.r WARNING @ Tue, 02 Aug 2022 19:31:55: #2 Since the d (69) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 02 Aug 2022 19:31:55: #2 You may need to consider one of the other alternative d(s): 4,69 WARNING @ Tue, 02 Aug 2022 19:31:55: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 02 Aug 2022 19:31:55: #3 Call peaks... INFO @ Tue, 02 Aug 2022 19:31:55: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 02 Aug 2022 19:32:03: 53000000 INFO @ Tue, 02 Aug 2022 19:32:03: 57000000 INFO @ Tue, 02 Aug 2022 19:32:13: 54000000 INFO @ Tue, 02 Aug 2022 19:32:13: 58000000 INFO @ Tue, 02 Aug 2022 19:32:22: 55000000 INFO @ Tue, 02 Aug 2022 19:32:24: 59000000 INFO @ Tue, 02 Aug 2022 19:32:31: 56000000 INFO @ Tue, 02 Aug 2022 19:32:35: 60000000 INFO @ Tue, 02 Aug 2022 19:32:40: 57000000 INFO @ Tue, 02 Aug 2022 19:32:42: #3 Call peaks for each chromosome... INFO @ Tue, 02 Aug 2022 19:32:45: 61000000 INFO @ Tue, 02 Aug 2022 19:32:49: #1 tag size is determined as 50 bps INFO @ Tue, 02 Aug 2022 19:32:49: #1 tag size = 50 INFO @ Tue, 02 Aug 2022 19:32:49: #1 total tags in treatment: 22170832 INFO @ Tue, 02 Aug 2022 19:32:49: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 19:32:49: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 19:32:49: 58000000 INFO @ Tue, 02 Aug 2022 19:32:49: #1 tags after filtering in treatment: 14956079 INFO @ Tue, 02 Aug 2022 19:32:49: #1 Redundant rate of treatment: 0.33 INFO @ Tue, 02 Aug 2022 19:32:49: #1 finished! INFO @ Tue, 02 Aug 2022 19:32:49: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 19:32:49: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 19:32:51: #2 number of paired peaks: 850 WARNING @ Tue, 02 Aug 2022 19:32:51: Fewer paired peaks (850) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 850 pairs to build model! INFO @ Tue, 02 Aug 2022 19:32:51: start model_add_line... INFO @ Tue, 02 Aug 2022 19:32:51: start X-correlation... INFO @ Tue, 02 Aug 2022 19:32:51: end of X-cor INFO @ Tue, 02 Aug 2022 19:32:51: #2 finished! INFO @ Tue, 02 Aug 2022 19:32:51: #2 predicted fragment length is 69 bps INFO @ Tue, 02 Aug 2022 19:32:51: #2 alternative fragment length(s) may be 4,69 bps INFO @ Tue, 02 Aug 2022 19:32:51: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX12178841/SRX12178841.05_model.r WARNING @ Tue, 02 Aug 2022 19:32:51: #2 Since the d (69) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 02 Aug 2022 19:32:51: #2 You may need to consider one of the other alternative d(s): 4,69 WARNING @ Tue, 02 Aug 2022 19:32:51: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 02 Aug 2022 19:32:51: #3 Call peaks... INFO @ Tue, 02 Aug 2022 19:32:51: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 02 Aug 2022 19:32:58: 59000000 INFO @ Tue, 02 Aug 2022 19:33:08: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX12178841/SRX12178841.10_peaks.xls INFO @ Tue, 02 Aug 2022 19:33:08: 60000000 INFO @ Tue, 02 Aug 2022 19:33:08: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX12178841/SRX12178841.10_peaks.narrowPeak INFO @ Tue, 02 Aug 2022 19:33:08: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX12178841/SRX12178841.10_summits.bed INFO @ Tue, 02 Aug 2022 19:33:08: Done! pass1 - making usageList (15 chroms): 3 millis pass2 - checking and writing primary data (8575 records, 4 fields): 14 millis CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 19:33:18: 61000000 INFO @ Tue, 02 Aug 2022 19:33:21: #1 tag size is determined as 50 bps INFO @ Tue, 02 Aug 2022 19:33:21: #1 tag size = 50 INFO @ Tue, 02 Aug 2022 19:33:21: #1 total tags in treatment: 22170832 INFO @ Tue, 02 Aug 2022 19:33:21: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 19:33:21: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 19:33:22: #1 tags after filtering in treatment: 14956079 INFO @ Tue, 02 Aug 2022 19:33:22: #1 Redundant rate of treatment: 0.33 INFO @ Tue, 02 Aug 2022 19:33:22: #1 finished! INFO @ Tue, 02 Aug 2022 19:33:22: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 19:33:22: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 19:33:23: #2 number of paired peaks: 850 WARNING @ Tue, 02 Aug 2022 19:33:23: Fewer paired peaks (850) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 850 pairs to build model! INFO @ Tue, 02 Aug 2022 19:33:23: start model_add_line... INFO @ Tue, 02 Aug 2022 19:33:24: start X-correlation... INFO @ Tue, 02 Aug 2022 19:33:24: end of X-cor INFO @ Tue, 02 Aug 2022 19:33:24: #2 finished! INFO @ Tue, 02 Aug 2022 19:33:24: #2 predicted fragment length is 69 bps INFO @ Tue, 02 Aug 2022 19:33:24: #2 alternative fragment length(s) may be 4,69 bps INFO @ Tue, 02 Aug 2022 19:33:24: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX12178841/SRX12178841.20_model.r WARNING @ Tue, 02 Aug 2022 19:33:24: #2 Since the d (69) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 02 Aug 2022 19:33:24: #2 You may need to consider one of the other alternative d(s): 4,69 WARNING @ Tue, 02 Aug 2022 19:33:24: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 02 Aug 2022 19:33:24: #3 Call peaks... INFO @ Tue, 02 Aug 2022 19:33:24: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 02 Aug 2022 19:33:34: #3 Call peaks for each chromosome... INFO @ Tue, 02 Aug 2022 19:33:59: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX12178841/SRX12178841.05_peaks.xls INFO @ Tue, 02 Aug 2022 19:33:59: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX12178841/SRX12178841.05_peaks.narrowPeak INFO @ Tue, 02 Aug 2022 19:33:59: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX12178841/SRX12178841.05_summits.bed INFO @ Tue, 02 Aug 2022 19:33:59: Done! pass1 - making usageList (15 chroms): 5 millis pass2 - checking and writing primary data (18213 records, 4 fields): 24 millis CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 19:34:06: #3 Call peaks for each chromosome... INFO @ Tue, 02 Aug 2022 19:34:29: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX12178841/SRX12178841.20_peaks.xls INFO @ Tue, 02 Aug 2022 19:34:29: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX12178841/SRX12178841.20_peaks.narrowPeak INFO @ Tue, 02 Aug 2022 19:34:29: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX12178841/SRX12178841.20_summits.bed INFO @ Tue, 02 Aug 2022 19:34:29: Done! pass1 - making usageList (13 chroms): 1 millis pass2 - checking and writing primary data (1032 records, 4 fields): 7 millis CompletedMACS2peakCalling