Job ID = 16439860 SRX = SRX12175815 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 39877108 spots for SRR15884954/SRR15884954.sra Written 39877108 spots for SRR15884954/SRR15884954.sra fastq に変換しました。 bowtie でマッピング中... Your job 16440929 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 01:35:40 39877108 reads; of these: 39877108 (100.00%) were paired; of these: 20046784 (50.27%) aligned concordantly 0 times 18031575 (45.22%) aligned concordantly exactly 1 time 1798749 (4.51%) aligned concordantly >1 times ---- 20046784 pairs aligned concordantly 0 times; of these: 5986059 (29.86%) aligned discordantly 1 time ---- 14060725 pairs aligned 0 times concordantly or discordantly; of these: 28121450 mates make up the pairs; of these: 25449719 (90.50%) aligned 0 times 1601752 (5.70%) aligned exactly 1 time 1069979 (3.80%) aligned >1 times 68.09% overall alignment rate Time searching: 01:35:40 Overall time: 01:35:40 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 40 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 6325547 / 25711940 = 0.2460 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 18:39:00: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX12175815/SRX12175815.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX12175815/SRX12175815.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX12175815/SRX12175815.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX12175815/SRX12175815.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 18:39:00: #1 read tag files... INFO @ Tue, 02 Aug 2022 18:39:00: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 18:39:12: 1000000 INFO @ Tue, 02 Aug 2022 18:39:23: 2000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 18:39:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX12175815/SRX12175815.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX12175815/SRX12175815.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX12175815/SRX12175815.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX12175815/SRX12175815.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 18:39:30: #1 read tag files... INFO @ Tue, 02 Aug 2022 18:39:30: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 18:39:34: 3000000 INFO @ Tue, 02 Aug 2022 18:39:40: 1000000 INFO @ Tue, 02 Aug 2022 18:39:46: 4000000 INFO @ Tue, 02 Aug 2022 18:39:48: 2000000 BedGraph に変換中... INFO @ Tue, 02 Aug 2022 18:39:57: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 18:39:57: 5000000 INFO @ Tue, 02 Aug 2022 18:39:59: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX12175815/SRX12175815.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX12175815/SRX12175815.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX12175815/SRX12175815.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX12175815/SRX12175815.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 18:39:59: #1 read tag files... INFO @ Tue, 02 Aug 2022 18:39:59: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 18:40:05: 4000000 INFO @ Tue, 02 Aug 2022 18:40:09: 6000000 INFO @ Tue, 02 Aug 2022 18:40:11: 1000000 INFO @ Tue, 02 Aug 2022 18:40:14: 5000000 INFO @ Tue, 02 Aug 2022 18:40:21: 7000000 INFO @ Tue, 02 Aug 2022 18:40:22: 2000000 INFO @ Tue, 02 Aug 2022 18:40:22: 6000000 INFO @ Tue, 02 Aug 2022 18:40:31: 7000000 INFO @ Tue, 02 Aug 2022 18:40:32: 8000000 INFO @ Tue, 02 Aug 2022 18:40:35: 3000000 INFO @ Tue, 02 Aug 2022 18:40:39: 8000000 INFO @ Tue, 02 Aug 2022 18:40:44: 9000000 INFO @ Tue, 02 Aug 2022 18:40:47: 4000000 INFO @ Tue, 02 Aug 2022 18:40:48: 9000000 INFO @ Tue, 02 Aug 2022 18:40:55: 10000000 INFO @ Tue, 02 Aug 2022 18:40:56: 5000000 INFO @ Tue, 02 Aug 2022 18:40:57: 10000000 INFO @ Tue, 02 Aug 2022 18:41:05: 6000000 INFO @ Tue, 02 Aug 2022 18:41:05: 11000000 INFO @ Tue, 02 Aug 2022 18:41:06: 11000000 INFO @ Tue, 02 Aug 2022 18:41:15: 7000000 INFO @ Tue, 02 Aug 2022 18:41:16: 12000000 INFO @ Tue, 02 Aug 2022 18:41:16: 12000000 INFO @ Tue, 02 Aug 2022 18:41:25: 8000000 INFO @ Tue, 02 Aug 2022 18:41:26: 13000000 INFO @ Tue, 02 Aug 2022 18:41:27: 13000000 INFO @ Tue, 02 Aug 2022 18:41:35: 9000000 INFO @ Tue, 02 Aug 2022 18:41:36: 14000000 INFO @ Tue, 02 Aug 2022 18:41:37: 14000000 INFO @ Tue, 02 Aug 2022 18:41:44: 10000000 INFO @ Tue, 02 Aug 2022 18:41:44: 15000000 INFO @ Tue, 02 Aug 2022 18:41:48: 15000000 INFO @ Tue, 02 Aug 2022 18:41:53: 11000000 INFO @ Tue, 02 Aug 2022 18:41:53: 16000000 INFO @ Tue, 02 Aug 2022 18:41:59: 16000000 INFO @ Tue, 02 Aug 2022 18:42:02: 12000000 INFO @ Tue, 02 Aug 2022 18:42:02: 17000000 INFO @ Tue, 02 Aug 2022 18:42:09: 17000000 INFO @ Tue, 02 Aug 2022 18:42:12: 18000000 INFO @ Tue, 02 Aug 2022 18:42:12: 13000000 INFO @ Tue, 02 Aug 2022 18:42:21: 18000000 INFO @ Tue, 02 Aug 2022 18:42:22: 19000000 INFO @ Tue, 02 Aug 2022 18:42:23: 14000000 INFO @ Tue, 02 Aug 2022 18:42:31: 19000000 INFO @ Tue, 02 Aug 2022 18:42:32: 20000000 INFO @ Tue, 02 Aug 2022 18:42:34: 15000000 INFO @ Tue, 02 Aug 2022 18:42:41: 21000000 INFO @ Tue, 02 Aug 2022 18:42:42: 20000000 INFO @ Tue, 02 Aug 2022 18:42:45: 16000000 INFO @ Tue, 02 Aug 2022 18:42:50: 22000000 INFO @ Tue, 02 Aug 2022 18:42:54: 21000000 INFO @ Tue, 02 Aug 2022 18:42:55: 17000000 INFO @ Tue, 02 Aug 2022 18:42:59: 23000000 INFO @ Tue, 02 Aug 2022 18:43:06: 18000000 INFO @ Tue, 02 Aug 2022 18:43:07: 22000000 INFO @ Tue, 02 Aug 2022 18:43:08: 24000000 INFO @ Tue, 02 Aug 2022 18:43:16: 19000000 INFO @ Tue, 02 Aug 2022 18:43:18: 25000000 INFO @ Tue, 02 Aug 2022 18:43:20: 23000000 INFO @ Tue, 02 Aug 2022 18:43:26: 20000000 INFO @ Tue, 02 Aug 2022 18:43:26: 26000000 INFO @ Tue, 02 Aug 2022 18:43:32: 24000000 INFO @ Tue, 02 Aug 2022 18:43:35: 21000000 INFO @ Tue, 02 Aug 2022 18:43:36: 27000000 INFO @ Tue, 02 Aug 2022 18:43:45: 25000000 INFO @ Tue, 02 Aug 2022 18:43:45: 28000000 INFO @ Tue, 02 Aug 2022 18:43:45: 22000000 INFO @ Tue, 02 Aug 2022 18:43:54: 29000000 INFO @ Tue, 02 Aug 2022 18:43:54: 23000000 INFO @ Tue, 02 Aug 2022 18:43:57: 26000000 INFO @ Tue, 02 Aug 2022 18:44:03: 30000000 INFO @ Tue, 02 Aug 2022 18:44:04: 24000000 INFO @ Tue, 02 Aug 2022 18:44:08: 27000000 INFO @ Tue, 02 Aug 2022 18:44:13: 31000000 INFO @ Tue, 02 Aug 2022 18:44:13: 25000000 INFO @ Tue, 02 Aug 2022 18:44:19: 28000000 INFO @ Tue, 02 Aug 2022 18:44:22: 32000000 INFO @ Tue, 02 Aug 2022 18:44:23: 26000000 INFO @ Tue, 02 Aug 2022 18:44:30: 29000000 INFO @ Tue, 02 Aug 2022 18:44:32: 27000000 INFO @ Tue, 02 Aug 2022 18:44:32: 33000000 INFO @ Tue, 02 Aug 2022 18:44:41: 28000000 INFO @ Tue, 02 Aug 2022 18:44:41: 30000000 INFO @ Tue, 02 Aug 2022 18:44:42: 34000000 INFO @ Tue, 02 Aug 2022 18:44:50: 29000000 INFO @ Tue, 02 Aug 2022 18:44:51: 35000000 INFO @ Tue, 02 Aug 2022 18:44:52: 31000000 INFO @ Tue, 02 Aug 2022 18:44:59: 30000000 INFO @ Tue, 02 Aug 2022 18:45:00: 36000000 INFO @ Tue, 02 Aug 2022 18:45:03: 32000000 INFO @ Tue, 02 Aug 2022 18:45:09: 31000000 INFO @ Tue, 02 Aug 2022 18:45:10: 37000000 INFO @ Tue, 02 Aug 2022 18:45:14: 33000000 INFO @ Tue, 02 Aug 2022 18:45:18: 32000000 INFO @ Tue, 02 Aug 2022 18:45:19: 38000000 INFO @ Tue, 02 Aug 2022 18:45:25: 34000000 INFO @ Tue, 02 Aug 2022 18:45:28: 33000000 INFO @ Tue, 02 Aug 2022 18:45:30: 39000000 INFO @ Tue, 02 Aug 2022 18:45:36: 35000000 INFO @ Tue, 02 Aug 2022 18:45:37: 34000000 INFO @ Tue, 02 Aug 2022 18:45:41: 40000000 INFO @ Tue, 02 Aug 2022 18:45:47: 36000000 INFO @ Tue, 02 Aug 2022 18:45:47: 35000000 INFO @ Tue, 02 Aug 2022 18:45:50: 41000000 INFO @ Tue, 02 Aug 2022 18:45:56: #1 tag size is determined as 150 bps INFO @ Tue, 02 Aug 2022 18:45:56: #1 tag size = 150 INFO @ Tue, 02 Aug 2022 18:45:56: #1 total tags in treatment: 14702790 INFO @ Tue, 02 Aug 2022 18:45:56: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 18:45:56: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 18:45:57: #1 tags after filtering in treatment: 8124206 INFO @ Tue, 02 Aug 2022 18:45:57: #1 Redundant rate of treatment: 0.45 INFO @ Tue, 02 Aug 2022 18:45:57: #1 finished! INFO @ Tue, 02 Aug 2022 18:45:57: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 18:45:57: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 18:45:57: 36000000 INFO @ Tue, 02 Aug 2022 18:45:57: 37000000 INFO @ Tue, 02 Aug 2022 18:45:58: #2 number of paired peaks: 6576 INFO @ Tue, 02 Aug 2022 18:45:58: start model_add_line... INFO @ Tue, 02 Aug 2022 18:45:58: start X-correlation... INFO @ Tue, 02 Aug 2022 18:45:58: end of X-cor INFO @ Tue, 02 Aug 2022 18:45:58: #2 finished! INFO @ Tue, 02 Aug 2022 18:45:58: #2 predicted fragment length is 249 bps INFO @ Tue, 02 Aug 2022 18:45:58: #2 alternative fragment length(s) may be 249 bps INFO @ Tue, 02 Aug 2022 18:45:58: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX12175815/SRX12175815.10_model.r WARNING @ Tue, 02 Aug 2022 18:45:58: #2 Since the d (249) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 02 Aug 2022 18:45:58: #2 You may need to consider one of the other alternative d(s): 249 WARNING @ Tue, 02 Aug 2022 18:45:58: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 02 Aug 2022 18:45:58: #3 Call peaks... INFO @ Tue, 02 Aug 2022 18:45:58: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 02 Aug 2022 18:46:05: 37000000 INFO @ Tue, 02 Aug 2022 18:46:08: 38000000 INFO @ Tue, 02 Aug 2022 18:46:14: 38000000 INFO @ Tue, 02 Aug 2022 18:46:18: 39000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 02 Aug 2022 18:46:22: 39000000 INFO @ Tue, 02 Aug 2022 18:46:23: #3 Call peaks for each chromosome... INFO @ Tue, 02 Aug 2022 18:46:29: 40000000 INFO @ Tue, 02 Aug 2022 18:46:31: 40000000 INFO @ Tue, 02 Aug 2022 18:46:34: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX12175815/SRX12175815.10_peaks.xls INFO @ Tue, 02 Aug 2022 18:46:34: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX12175815/SRX12175815.10_peaks.narrowPeak INFO @ Tue, 02 Aug 2022 18:46:34: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX12175815/SRX12175815.10_summits.bed INFO @ Tue, 02 Aug 2022 18:46:34: Done! pass1 - making usageList (15 chroms): 2 millis pass2 - checking and writing primary data (7612 records, 4 fields): 25 millis CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 18:46:39: 41000000 INFO @ Tue, 02 Aug 2022 18:46:39: 41000000 INFO @ Tue, 02 Aug 2022 18:46:45: #1 tag size is determined as 150 bps INFO @ Tue, 02 Aug 2022 18:46:45: #1 tag size = 150 INFO @ Tue, 02 Aug 2022 18:46:45: #1 total tags in treatment: 14702790 INFO @ Tue, 02 Aug 2022 18:46:45: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 18:46:45: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 18:46:45: #1 tags after filtering in treatment: 8124206 INFO @ Tue, 02 Aug 2022 18:46:45: #1 Redundant rate of treatment: 0.45 INFO @ Tue, 02 Aug 2022 18:46:45: #1 finished! INFO @ Tue, 02 Aug 2022 18:46:45: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 18:46:45: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 18:46:46: #2 number of paired peaks: 6576 INFO @ Tue, 02 Aug 2022 18:46:46: start model_add_line... INFO @ Tue, 02 Aug 2022 18:46:46: #1 tag size is determined as 150 bps INFO @ Tue, 02 Aug 2022 18:46:46: #1 tag size = 150 INFO @ Tue, 02 Aug 2022 18:46:46: #1 total tags in treatment: 14702790 INFO @ Tue, 02 Aug 2022 18:46:46: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 18:46:46: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 18:46:46: start X-correlation... INFO @ Tue, 02 Aug 2022 18:46:46: end of X-cor INFO @ Tue, 02 Aug 2022 18:46:46: #2 finished! INFO @ Tue, 02 Aug 2022 18:46:46: #2 predicted fragment length is 249 bps INFO @ Tue, 02 Aug 2022 18:46:46: #2 alternative fragment length(s) may be 249 bps INFO @ Tue, 02 Aug 2022 18:46:46: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX12175815/SRX12175815.20_model.r WARNING @ Tue, 02 Aug 2022 18:46:46: #2 Since the d (249) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 02 Aug 2022 18:46:46: #2 You may need to consider one of the other alternative d(s): 249 WARNING @ Tue, 02 Aug 2022 18:46:46: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 02 Aug 2022 18:46:46: #3 Call peaks... INFO @ Tue, 02 Aug 2022 18:46:46: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 02 Aug 2022 18:46:47: #1 tags after filtering in treatment: 8124206 INFO @ Tue, 02 Aug 2022 18:46:47: #1 Redundant rate of treatment: 0.45 INFO @ Tue, 02 Aug 2022 18:46:47: #1 finished! INFO @ Tue, 02 Aug 2022 18:46:47: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 18:46:47: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 18:46:48: #2 number of paired peaks: 6576 INFO @ Tue, 02 Aug 2022 18:46:48: start model_add_line... INFO @ Tue, 02 Aug 2022 18:46:48: start X-correlation... INFO @ Tue, 02 Aug 2022 18:46:48: end of X-cor INFO @ Tue, 02 Aug 2022 18:46:48: #2 finished! INFO @ Tue, 02 Aug 2022 18:46:48: #2 predicted fragment length is 249 bps INFO @ Tue, 02 Aug 2022 18:46:48: #2 alternative fragment length(s) may be 249 bps INFO @ Tue, 02 Aug 2022 18:46:48: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX12175815/SRX12175815.05_model.r WARNING @ Tue, 02 Aug 2022 18:46:48: #2 Since the d (249) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 02 Aug 2022 18:46:48: #2 You may need to consider one of the other alternative d(s): 249 WARNING @ Tue, 02 Aug 2022 18:46:48: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 02 Aug 2022 18:46:48: #3 Call peaks... INFO @ Tue, 02 Aug 2022 18:46:48: #3 Pre-compute pvalue-qvalue table... BigWig に変換しました。 INFO @ Tue, 02 Aug 2022 18:47:09: #3 Call peaks for each chromosome... INFO @ Tue, 02 Aug 2022 18:47:11: #3 Call peaks for each chromosome... INFO @ Tue, 02 Aug 2022 18:47:19: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX12175815/SRX12175815.20_peaks.xls INFO @ Tue, 02 Aug 2022 18:47:19: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX12175815/SRX12175815.20_peaks.narrowPeak INFO @ Tue, 02 Aug 2022 18:47:19: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX12175815/SRX12175815.20_summits.bed INFO @ Tue, 02 Aug 2022 18:47:19: Done! pass1 - making usageList (14 chroms): 2 millis pass2 - checking and writing primary data (5079 records, 4 fields): 22 millis CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 18:47:22: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX12175815/SRX12175815.05_peaks.xls INFO @ Tue, 02 Aug 2022 18:47:22: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX12175815/SRX12175815.05_peaks.narrowPeak INFO @ Tue, 02 Aug 2022 18:47:22: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX12175815/SRX12175815.05_summits.bed INFO @ Tue, 02 Aug 2022 18:47:22: Done! pass1 - making usageList (15 chroms): 3 millis pass2 - checking and writing primary data (9853 records, 4 fields): 25 millis CompletedMACS2peakCalling