Job ID = 1293812 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 42,058,541 reads read : 42,058,541 reads written : 42,058,541 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:25:17 42058541 reads; of these: 42058541 (100.00%) were unpaired; of these: 1090195 (2.59%) aligned 0 times 15199646 (36.14%) aligned exactly 1 time 25768700 (61.27%) aligned >1 times 97.41% overall alignment rate Time searching: 00:25:17 Overall time: 00:25:17 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 7878825 / 40968346 = 0.1923 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 03 Jun 2019 03:24:38: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX1167418/SRX1167418.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX1167418/SRX1167418.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX1167418/SRX1167418.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX1167418/SRX1167418.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 03:24:38: #1 read tag files... INFO @ Mon, 03 Jun 2019 03:24:38: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 03:24:38: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX1167418/SRX1167418.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX1167418/SRX1167418.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX1167418/SRX1167418.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX1167418/SRX1167418.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 03:24:38: #1 read tag files... INFO @ Mon, 03 Jun 2019 03:24:38: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 03:24:38: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX1167418/SRX1167418.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX1167418/SRX1167418.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX1167418/SRX1167418.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX1167418/SRX1167418.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 03:24:38: #1 read tag files... INFO @ Mon, 03 Jun 2019 03:24:38: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 03:24:45: 1000000 INFO @ Mon, 03 Jun 2019 03:24:46: 1000000 INFO @ Mon, 03 Jun 2019 03:24:47: 1000000 INFO @ Mon, 03 Jun 2019 03:24:52: 2000000 INFO @ Mon, 03 Jun 2019 03:24:53: 2000000 INFO @ Mon, 03 Jun 2019 03:24:55: 2000000 INFO @ Mon, 03 Jun 2019 03:24:58: 3000000 INFO @ Mon, 03 Jun 2019 03:25:00: 3000000 INFO @ Mon, 03 Jun 2019 03:25:03: 3000000 INFO @ Mon, 03 Jun 2019 03:25:04: 4000000 INFO @ Mon, 03 Jun 2019 03:25:07: 4000000 INFO @ Mon, 03 Jun 2019 03:25:11: 4000000 INFO @ Mon, 03 Jun 2019 03:25:11: 5000000 INFO @ Mon, 03 Jun 2019 03:25:13: 5000000 INFO @ Mon, 03 Jun 2019 03:25:17: 6000000 INFO @ Mon, 03 Jun 2019 03:25:19: 5000000 INFO @ Mon, 03 Jun 2019 03:25:20: 6000000 INFO @ Mon, 03 Jun 2019 03:25:23: 7000000 INFO @ Mon, 03 Jun 2019 03:25:27: 6000000 INFO @ Mon, 03 Jun 2019 03:25:27: 7000000 INFO @ Mon, 03 Jun 2019 03:25:30: 8000000 INFO @ Mon, 03 Jun 2019 03:25:34: 8000000 INFO @ Mon, 03 Jun 2019 03:25:34: 7000000 INFO @ Mon, 03 Jun 2019 03:25:36: 9000000 INFO @ Mon, 03 Jun 2019 03:25:41: 9000000 INFO @ Mon, 03 Jun 2019 03:25:42: 8000000 INFO @ Mon, 03 Jun 2019 03:25:43: 10000000 INFO @ Mon, 03 Jun 2019 03:25:48: 10000000 INFO @ Mon, 03 Jun 2019 03:25:49: 11000000 INFO @ Mon, 03 Jun 2019 03:25:50: 9000000 INFO @ Mon, 03 Jun 2019 03:25:55: 11000000 INFO @ Mon, 03 Jun 2019 03:25:55: 12000000 INFO @ Mon, 03 Jun 2019 03:25:58: 10000000 INFO @ Mon, 03 Jun 2019 03:26:01: 12000000 INFO @ Mon, 03 Jun 2019 03:26:02: 13000000 INFO @ Mon, 03 Jun 2019 03:26:06: 11000000 INFO @ Mon, 03 Jun 2019 03:26:08: 14000000 INFO @ Mon, 03 Jun 2019 03:26:08: 13000000 INFO @ Mon, 03 Jun 2019 03:26:13: 12000000 INFO @ Mon, 03 Jun 2019 03:26:14: 15000000 INFO @ Mon, 03 Jun 2019 03:26:15: 14000000 INFO @ Mon, 03 Jun 2019 03:26:21: 16000000 INFO @ Mon, 03 Jun 2019 03:26:21: 13000000 INFO @ Mon, 03 Jun 2019 03:26:22: 15000000 INFO @ Mon, 03 Jun 2019 03:26:27: 17000000 INFO @ Mon, 03 Jun 2019 03:26:29: 16000000 INFO @ Mon, 03 Jun 2019 03:26:29: 14000000 INFO @ Mon, 03 Jun 2019 03:26:33: 18000000 INFO @ Mon, 03 Jun 2019 03:26:35: 17000000 INFO @ Mon, 03 Jun 2019 03:26:37: 15000000 INFO @ Mon, 03 Jun 2019 03:26:39: 19000000 INFO @ Mon, 03 Jun 2019 03:26:42: 18000000 INFO @ Mon, 03 Jun 2019 03:26:44: 16000000 INFO @ Mon, 03 Jun 2019 03:26:46: 20000000 INFO @ Mon, 03 Jun 2019 03:26:49: 19000000 INFO @ Mon, 03 Jun 2019 03:26:52: 21000000 INFO @ Mon, 03 Jun 2019 03:26:52: 17000000 INFO @ Mon, 03 Jun 2019 03:26:56: 20000000 INFO @ Mon, 03 Jun 2019 03:26:58: 22000000 INFO @ Mon, 03 Jun 2019 03:27:00: 18000000 INFO @ Mon, 03 Jun 2019 03:27:02: 21000000 INFO @ Mon, 03 Jun 2019 03:27:04: 23000000 INFO @ Mon, 03 Jun 2019 03:27:08: 19000000 INFO @ Mon, 03 Jun 2019 03:27:09: 22000000 INFO @ Mon, 03 Jun 2019 03:27:11: 24000000 INFO @ Mon, 03 Jun 2019 03:27:15: 20000000 INFO @ Mon, 03 Jun 2019 03:27:16: 23000000 INFO @ Mon, 03 Jun 2019 03:27:17: 25000000 INFO @ Mon, 03 Jun 2019 03:27:22: 24000000 INFO @ Mon, 03 Jun 2019 03:27:23: 21000000 INFO @ Mon, 03 Jun 2019 03:27:23: 26000000 INFO @ Mon, 03 Jun 2019 03:27:29: 25000000 INFO @ Mon, 03 Jun 2019 03:27:29: 27000000 INFO @ Mon, 03 Jun 2019 03:27:30: 22000000 INFO @ Mon, 03 Jun 2019 03:27:36: 28000000 INFO @ Mon, 03 Jun 2019 03:27:36: 26000000 INFO @ Mon, 03 Jun 2019 03:27:38: 23000000 INFO @ Mon, 03 Jun 2019 03:27:42: 29000000 INFO @ Mon, 03 Jun 2019 03:27:42: 27000000 INFO @ Mon, 03 Jun 2019 03:27:45: 24000000 INFO @ Mon, 03 Jun 2019 03:27:48: 30000000 INFO @ Mon, 03 Jun 2019 03:27:49: 28000000 INFO @ Mon, 03 Jun 2019 03:27:53: 25000000 INFO @ Mon, 03 Jun 2019 03:27:54: 31000000 INFO @ Mon, 03 Jun 2019 03:27:56: 29000000 INFO @ Mon, 03 Jun 2019 03:28:00: 26000000 INFO @ Mon, 03 Jun 2019 03:28:01: 32000000 INFO @ Mon, 03 Jun 2019 03:28:02: 30000000 INFO @ Mon, 03 Jun 2019 03:28:07: 33000000 INFO @ Mon, 03 Jun 2019 03:28:08: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 03:28:08: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 03:28:08: #1 total tags in treatment: 33089521 INFO @ Mon, 03 Jun 2019 03:28:08: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 03:28:08: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 03:28:08: 27000000 INFO @ Mon, 03 Jun 2019 03:28:09: #1 tags after filtering in treatment: 33089521 INFO @ Mon, 03 Jun 2019 03:28:09: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 03:28:09: #1 finished! INFO @ Mon, 03 Jun 2019 03:28:09: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 03:28:09: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 03:28:09: 31000000 INFO @ Mon, 03 Jun 2019 03:28:11: #2 number of paired peaks: 43 WARNING @ Mon, 03 Jun 2019 03:28:11: Too few paired peaks (43) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 03:28:11: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX1167418/SRX1167418.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX1167418/SRX1167418.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX1167418/SRX1167418.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX1167418/SRX1167418.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 03:28:16: 28000000 INFO @ Mon, 03 Jun 2019 03:28:16: 32000000 INFO @ Mon, 03 Jun 2019 03:28:22: 33000000 INFO @ Mon, 03 Jun 2019 03:28:23: 29000000 INFO @ Mon, 03 Jun 2019 03:28:23: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 03:28:23: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 03:28:23: #1 total tags in treatment: 33089521 INFO @ Mon, 03 Jun 2019 03:28:23: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 03:28:23: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 03:28:24: #1 tags after filtering in treatment: 33089521 INFO @ Mon, 03 Jun 2019 03:28:24: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 03:28:24: #1 finished! INFO @ Mon, 03 Jun 2019 03:28:24: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 03:28:24: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 03:28:27: #2 number of paired peaks: 43 WARNING @ Mon, 03 Jun 2019 03:28:27: Too few paired peaks (43) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 03:28:27: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX1167418/SRX1167418.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX1167418/SRX1167418.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX1167418/SRX1167418.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX1167418/SRX1167418.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 03:28:31: 30000000 INFO @ Mon, 03 Jun 2019 03:28:38: 31000000 INFO @ Mon, 03 Jun 2019 03:28:46: 32000000 INFO @ Mon, 03 Jun 2019 03:28:53: 33000000 INFO @ Mon, 03 Jun 2019 03:28:54: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 03:28:54: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 03:28:54: #1 total tags in treatment: 33089521 INFO @ Mon, 03 Jun 2019 03:28:54: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 03:28:54: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 03:28:55: #1 tags after filtering in treatment: 33089521 INFO @ Mon, 03 Jun 2019 03:28:55: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 03:28:55: #1 finished! INFO @ Mon, 03 Jun 2019 03:28:55: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 03:28:55: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 03:28:58: #2 number of paired peaks: 43 WARNING @ Mon, 03 Jun 2019 03:28:58: Too few paired peaks (43) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 03:28:58: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX1167418/SRX1167418.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX1167418/SRX1167418.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX1167418/SRX1167418.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX1167418/SRX1167418.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。