Job ID = 1293811 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 41,046,866 reads read : 41,046,866 reads written : 41,046,866 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:27:06 41046866 reads; of these: 41046866 (100.00%) were unpaired; of these: 774727 (1.89%) aligned 0 times 12893096 (31.41%) aligned exactly 1 time 27379043 (66.70%) aligned >1 times 98.11% overall alignment rate Time searching: 00:27:06 Overall time: 00:27:06 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 8586878 / 40272139 = 0.2132 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 03 Jun 2019 03:24:17: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX1167417/SRX1167417.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX1167417/SRX1167417.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX1167417/SRX1167417.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX1167417/SRX1167417.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 03:24:17: #1 read tag files... INFO @ Mon, 03 Jun 2019 03:24:17: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 03:24:17: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX1167417/SRX1167417.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX1167417/SRX1167417.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX1167417/SRX1167417.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX1167417/SRX1167417.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 03:24:17: #1 read tag files... INFO @ Mon, 03 Jun 2019 03:24:17: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 03:24:17: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX1167417/SRX1167417.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX1167417/SRX1167417.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX1167417/SRX1167417.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX1167417/SRX1167417.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 03:24:17: #1 read tag files... INFO @ Mon, 03 Jun 2019 03:24:17: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 03:24:24: 1000000 INFO @ Mon, 03 Jun 2019 03:24:25: 1000000 INFO @ Mon, 03 Jun 2019 03:24:25: 1000000 INFO @ Mon, 03 Jun 2019 03:24:31: 2000000 INFO @ Mon, 03 Jun 2019 03:24:32: 2000000 INFO @ Mon, 03 Jun 2019 03:24:32: 2000000 INFO @ Mon, 03 Jun 2019 03:24:38: 3000000 INFO @ Mon, 03 Jun 2019 03:24:39: 3000000 INFO @ Mon, 03 Jun 2019 03:24:40: 3000000 INFO @ Mon, 03 Jun 2019 03:24:46: 4000000 INFO @ Mon, 03 Jun 2019 03:24:47: 4000000 INFO @ Mon, 03 Jun 2019 03:24:47: 4000000 INFO @ Mon, 03 Jun 2019 03:24:53: 5000000 INFO @ Mon, 03 Jun 2019 03:24:54: 5000000 INFO @ Mon, 03 Jun 2019 03:24:55: 5000000 INFO @ Mon, 03 Jun 2019 03:25:00: 6000000 INFO @ Mon, 03 Jun 2019 03:25:02: 6000000 INFO @ Mon, 03 Jun 2019 03:25:02: 6000000 INFO @ Mon, 03 Jun 2019 03:25:07: 7000000 INFO @ Mon, 03 Jun 2019 03:25:09: 7000000 INFO @ Mon, 03 Jun 2019 03:25:10: 7000000 INFO @ Mon, 03 Jun 2019 03:25:14: 8000000 INFO @ Mon, 03 Jun 2019 03:25:17: 8000000 INFO @ Mon, 03 Jun 2019 03:25:17: 8000000 INFO @ Mon, 03 Jun 2019 03:25:21: 9000000 INFO @ Mon, 03 Jun 2019 03:25:24: 9000000 INFO @ Mon, 03 Jun 2019 03:25:25: 9000000 INFO @ Mon, 03 Jun 2019 03:25:28: 10000000 INFO @ Mon, 03 Jun 2019 03:25:31: 10000000 INFO @ Mon, 03 Jun 2019 03:25:32: 10000000 INFO @ Mon, 03 Jun 2019 03:25:35: 11000000 INFO @ Mon, 03 Jun 2019 03:25:39: 11000000 INFO @ Mon, 03 Jun 2019 03:25:40: 11000000 INFO @ Mon, 03 Jun 2019 03:25:42: 12000000 INFO @ Mon, 03 Jun 2019 03:25:46: 12000000 INFO @ Mon, 03 Jun 2019 03:25:47: 12000000 INFO @ Mon, 03 Jun 2019 03:25:49: 13000000 INFO @ Mon, 03 Jun 2019 03:25:53: 13000000 INFO @ Mon, 03 Jun 2019 03:25:54: 13000000 INFO @ Mon, 03 Jun 2019 03:25:56: 14000000 INFO @ Mon, 03 Jun 2019 03:26:01: 14000000 INFO @ Mon, 03 Jun 2019 03:26:02: 14000000 INFO @ Mon, 03 Jun 2019 03:26:03: 15000000 INFO @ Mon, 03 Jun 2019 03:26:08: 15000000 INFO @ Mon, 03 Jun 2019 03:26:09: 15000000 INFO @ Mon, 03 Jun 2019 03:26:10: 16000000 INFO @ Mon, 03 Jun 2019 03:26:15: 16000000 INFO @ Mon, 03 Jun 2019 03:26:16: 16000000 INFO @ Mon, 03 Jun 2019 03:26:17: 17000000 INFO @ Mon, 03 Jun 2019 03:26:22: 17000000 INFO @ Mon, 03 Jun 2019 03:26:23: 17000000 INFO @ Mon, 03 Jun 2019 03:26:24: 18000000 INFO @ Mon, 03 Jun 2019 03:26:29: 18000000 INFO @ Mon, 03 Jun 2019 03:26:31: 18000000 INFO @ Mon, 03 Jun 2019 03:26:31: 19000000 INFO @ Mon, 03 Jun 2019 03:26:37: 19000000 INFO @ Mon, 03 Jun 2019 03:26:38: 20000000 INFO @ Mon, 03 Jun 2019 03:26:38: 19000000 INFO @ Mon, 03 Jun 2019 03:26:44: 20000000 INFO @ Mon, 03 Jun 2019 03:26:44: 21000000 INFO @ Mon, 03 Jun 2019 03:26:45: 20000000 INFO @ Mon, 03 Jun 2019 03:26:51: 22000000 INFO @ Mon, 03 Jun 2019 03:26:51: 21000000 INFO @ Mon, 03 Jun 2019 03:26:52: 21000000 INFO @ Mon, 03 Jun 2019 03:26:58: 23000000 INFO @ Mon, 03 Jun 2019 03:26:58: 22000000 INFO @ Mon, 03 Jun 2019 03:27:00: 22000000 INFO @ Mon, 03 Jun 2019 03:27:05: 23000000 INFO @ Mon, 03 Jun 2019 03:27:05: 24000000 INFO @ Mon, 03 Jun 2019 03:27:07: 23000000 INFO @ Mon, 03 Jun 2019 03:27:12: 24000000 INFO @ Mon, 03 Jun 2019 03:27:13: 25000000 INFO @ Mon, 03 Jun 2019 03:27:15: 24000000 INFO @ Mon, 03 Jun 2019 03:27:19: 25000000 INFO @ Mon, 03 Jun 2019 03:27:19: 26000000 INFO @ Mon, 03 Jun 2019 03:27:22: 25000000 INFO @ Mon, 03 Jun 2019 03:27:26: 27000000 INFO @ Mon, 03 Jun 2019 03:27:27: 26000000 INFO @ Mon, 03 Jun 2019 03:27:30: 26000000 INFO @ Mon, 03 Jun 2019 03:27:33: 28000000 INFO @ Mon, 03 Jun 2019 03:27:34: 27000000 INFO @ Mon, 03 Jun 2019 03:27:37: 27000000 INFO @ Mon, 03 Jun 2019 03:27:41: 29000000 INFO @ Mon, 03 Jun 2019 03:27:41: 28000000 INFO @ Mon, 03 Jun 2019 03:27:45: 28000000 INFO @ Mon, 03 Jun 2019 03:27:48: 30000000 INFO @ Mon, 03 Jun 2019 03:27:48: 29000000 INFO @ Mon, 03 Jun 2019 03:27:52: 29000000 INFO @ Mon, 03 Jun 2019 03:27:55: 31000000 INFO @ Mon, 03 Jun 2019 03:27:56: 30000000 INFO @ Mon, 03 Jun 2019 03:27:59: 30000000 INFO @ Mon, 03 Jun 2019 03:28:00: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 03:28:00: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 03:28:00: #1 total tags in treatment: 31685261 INFO @ Mon, 03 Jun 2019 03:28:00: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 03:28:00: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 03:28:00: #1 tags after filtering in treatment: 31685261 INFO @ Mon, 03 Jun 2019 03:28:00: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 03:28:00: #1 finished! INFO @ Mon, 03 Jun 2019 03:28:00: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 03:28:00: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 03:28:03: 31000000 INFO @ Mon, 03 Jun 2019 03:28:03: #2 number of paired peaks: 74 WARNING @ Mon, 03 Jun 2019 03:28:03: Too few paired peaks (74) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 03:28:03: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX1167417/SRX1167417.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX1167417/SRX1167417.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX1167417/SRX1167417.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX1167417/SRX1167417.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 03:28:07: 31000000 INFO @ Mon, 03 Jun 2019 03:28:08: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 03:28:08: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 03:28:08: #1 total tags in treatment: 31685261 INFO @ Mon, 03 Jun 2019 03:28:08: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 03:28:08: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 03:28:09: #1 tags after filtering in treatment: 31685261 INFO @ Mon, 03 Jun 2019 03:28:09: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 03:28:09: #1 finished! INFO @ Mon, 03 Jun 2019 03:28:09: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 03:28:09: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 03:28:12: #2 number of paired peaks: 74 WARNING @ Mon, 03 Jun 2019 03:28:12: Too few paired peaks (74) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 03:28:12: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX1167417/SRX1167417.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX1167417/SRX1167417.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX1167417/SRX1167417.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX1167417/SRX1167417.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 03:28:12: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 03:28:12: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 03:28:12: #1 total tags in treatment: 31685261 INFO @ Mon, 03 Jun 2019 03:28:12: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 03:28:12: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 03:28:13: #1 tags after filtering in treatment: 31685261 INFO @ Mon, 03 Jun 2019 03:28:13: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 03:28:13: #1 finished! INFO @ Mon, 03 Jun 2019 03:28:13: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 03:28:13: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 03:28:15: #2 number of paired peaks: 74 WARNING @ Mon, 03 Jun 2019 03:28:15: Too few paired peaks (74) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 03:28:15: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX1167417/SRX1167417.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX1167417/SRX1167417.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX1167417/SRX1167417.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX1167417/SRX1167417.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。