Job ID = 16439624 SRX = SRX11548851 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 8294957 spots for SRR15243022/SRR15243022.sra Written 8294957 spots for SRR15243022/SRR15243022.sra fastq に変換しました。 bowtie でマッピング中... Your job 16439728 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:15:25 8294957 reads; of these: 8294957 (100.00%) were paired; of these: 896433 (10.81%) aligned concordantly 0 times 5881588 (70.91%) aligned concordantly exactly 1 time 1516936 (18.29%) aligned concordantly >1 times ---- 896433 pairs aligned concordantly 0 times; of these: 124596 (13.90%) aligned discordantly 1 time ---- 771837 pairs aligned 0 times concordantly or discordantly; of these: 1543674 mates make up the pairs; of these: 1198031 (77.61%) aligned 0 times 184603 (11.96%) aligned exactly 1 time 161040 (10.43%) aligned >1 times 92.78% overall alignment rate Time searching: 00:15:25 Overall time: 00:15:25 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 238342 / 7516179 = 0.0317 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 16:05:12: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX11548851/SRX11548851.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX11548851/SRX11548851.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX11548851/SRX11548851.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX11548851/SRX11548851.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 16:05:12: #1 read tag files... INFO @ Tue, 02 Aug 2022 16:05:12: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 16:05:19: 1000000 INFO @ Tue, 02 Aug 2022 16:05:26: 2000000 INFO @ Tue, 02 Aug 2022 16:05:32: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 16:05:39: 4000000 INFO @ Tue, 02 Aug 2022 16:05:40: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX11548851/SRX11548851.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX11548851/SRX11548851.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX11548851/SRX11548851.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX11548851/SRX11548851.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 16:05:40: #1 read tag files... INFO @ Tue, 02 Aug 2022 16:05:40: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 16:05:46: 5000000 INFO @ Tue, 02 Aug 2022 16:05:47: 1000000 INFO @ Tue, 02 Aug 2022 16:05:53: 6000000 INFO @ Tue, 02 Aug 2022 16:05:54: 2000000 INFO @ Tue, 02 Aug 2022 16:06:01: 7000000 INFO @ Tue, 02 Aug 2022 16:06:01: 3000000 INFO @ Tue, 02 Aug 2022 16:06:08: 4000000 INFO @ Tue, 02 Aug 2022 16:06:08: 8000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 16:06:10: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX11548851/SRX11548851.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX11548851/SRX11548851.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX11548851/SRX11548851.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX11548851/SRX11548851.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 16:06:10: #1 read tag files... INFO @ Tue, 02 Aug 2022 16:06:10: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 16:06:14: 5000000 INFO @ Tue, 02 Aug 2022 16:06:15: 9000000 INFO @ Tue, 02 Aug 2022 16:06:18: 1000000 INFO @ Tue, 02 Aug 2022 16:06:21: 6000000 INFO @ Tue, 02 Aug 2022 16:06:22: 10000000 INFO @ Tue, 02 Aug 2022 16:06:24: 2000000 INFO @ Tue, 02 Aug 2022 16:06:28: 7000000 INFO @ Tue, 02 Aug 2022 16:06:30: 11000000 INFO @ Tue, 02 Aug 2022 16:06:31: 3000000 INFO @ Tue, 02 Aug 2022 16:06:35: 8000000 INFO @ Tue, 02 Aug 2022 16:06:37: 12000000 INFO @ Tue, 02 Aug 2022 16:06:38: 4000000 INFO @ Tue, 02 Aug 2022 16:06:42: 9000000 INFO @ Tue, 02 Aug 2022 16:06:44: 13000000 INFO @ Tue, 02 Aug 2022 16:06:45: 5000000 INFO @ Tue, 02 Aug 2022 16:06:49: 10000000 INFO @ Tue, 02 Aug 2022 16:06:52: 14000000 INFO @ Tue, 02 Aug 2022 16:06:52: 6000000 INFO @ Tue, 02 Aug 2022 16:06:55: 11000000 INFO @ Tue, 02 Aug 2022 16:06:58: #1 tag size is determined as 75 bps INFO @ Tue, 02 Aug 2022 16:06:58: #1 tag size = 75 INFO @ Tue, 02 Aug 2022 16:06:58: #1 total tags in treatment: 7161355 INFO @ Tue, 02 Aug 2022 16:06:58: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 16:06:58: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 16:06:58: #1 tags after filtering in treatment: 6975535 INFO @ Tue, 02 Aug 2022 16:06:58: #1 Redundant rate of treatment: 0.03 INFO @ Tue, 02 Aug 2022 16:06:58: #1 finished! INFO @ Tue, 02 Aug 2022 16:06:58: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 16:06:58: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 16:06:59: 7000000 INFO @ Tue, 02 Aug 2022 16:06:59: #2 number of paired peaks: 50 WARNING @ Tue, 02 Aug 2022 16:06:59: Too few paired peaks (50) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 16:06:59: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX11548851/SRX11548851.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX11548851/SRX11548851.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX11548851/SRX11548851.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX11548851/SRX11548851.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 16:07:02: 12000000 INFO @ Tue, 02 Aug 2022 16:07:05: 8000000 INFO @ Tue, 02 Aug 2022 16:07:09: 13000000 INFO @ Tue, 02 Aug 2022 16:07:12: 9000000 INFO @ Tue, 02 Aug 2022 16:07:15: 14000000 INFO @ Tue, 02 Aug 2022 16:07:18: 10000000 INFO @ Tue, 02 Aug 2022 16:07:21: #1 tag size is determined as 75 bps INFO @ Tue, 02 Aug 2022 16:07:21: #1 tag size = 75 INFO @ Tue, 02 Aug 2022 16:07:21: #1 total tags in treatment: 7161355 INFO @ Tue, 02 Aug 2022 16:07:21: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 16:07:21: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 16:07:21: #1 tags after filtering in treatment: 6975535 INFO @ Tue, 02 Aug 2022 16:07:21: #1 Redundant rate of treatment: 0.03 INFO @ Tue, 02 Aug 2022 16:07:21: #1 finished! INFO @ Tue, 02 Aug 2022 16:07:21: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 16:07:21: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 16:07:22: #2 number of paired peaks: 50 WARNING @ Tue, 02 Aug 2022 16:07:22: Too few paired peaks (50) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 16:07:22: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX11548851/SRX11548851.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX11548851/SRX11548851.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX11548851/SRX11548851.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX11548851/SRX11548851.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 16:07:24: 11000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 02 Aug 2022 16:07:31: 12000000 INFO @ Tue, 02 Aug 2022 16:07:37: 13000000 INFO @ Tue, 02 Aug 2022 16:07:43: 14000000 INFO @ Tue, 02 Aug 2022 16:07:48: #1 tag size is determined as 75 bps INFO @ Tue, 02 Aug 2022 16:07:48: #1 tag size = 75 INFO @ Tue, 02 Aug 2022 16:07:48: #1 total tags in treatment: 7161355 INFO @ Tue, 02 Aug 2022 16:07:48: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 16:07:48: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 16:07:49: #1 tags after filtering in treatment: 6975535 INFO @ Tue, 02 Aug 2022 16:07:49: #1 Redundant rate of treatment: 0.03 INFO @ Tue, 02 Aug 2022 16:07:49: #1 finished! INFO @ Tue, 02 Aug 2022 16:07:49: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 16:07:49: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 16:07:49: #2 number of paired peaks: 50 WARNING @ Tue, 02 Aug 2022 16:07:49: Too few paired peaks (50) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 16:07:49: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX11548851/SRX11548851.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX11548851/SRX11548851.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX11548851/SRX11548851.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX11548851/SRX11548851.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。