Job ID = 16439611 SRX = SRX11548840 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 17672069 spots for SRR15243011/SRR15243011.sra Written 17672069 spots for SRR15243011/SRR15243011.sra fastq に変換しました。 bowtie でマッピング中... Your job 16440010 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:50:22 17672069 reads; of these: 17672069 (100.00%) were paired; of these: 3016892 (17.07%) aligned concordantly 0 times 10685225 (60.46%) aligned concordantly exactly 1 time 3969952 (22.46%) aligned concordantly >1 times ---- 3016892 pairs aligned concordantly 0 times; of these: 291045 (9.65%) aligned discordantly 1 time ---- 2725847 pairs aligned 0 times concordantly or discordantly; of these: 5451694 mates make up the pairs; of these: 4783010 (87.73%) aligned 0 times 353063 (6.48%) aligned exactly 1 time 315621 (5.79%) aligned >1 times 86.47% overall alignment rate Time searching: 00:50:22 Overall time: 00:50:22 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 16 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 512008 / 14895644 = 0.0344 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 16:46:45: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX11548840/SRX11548840.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX11548840/SRX11548840.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX11548840/SRX11548840.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX11548840/SRX11548840.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 16:46:45: #1 read tag files... INFO @ Tue, 02 Aug 2022 16:46:45: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 16:46:52: 1000000 INFO @ Tue, 02 Aug 2022 16:46:59: 2000000 INFO @ Tue, 02 Aug 2022 16:47:07: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 16:47:14: 4000000 INFO @ Tue, 02 Aug 2022 16:47:14: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX11548840/SRX11548840.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX11548840/SRX11548840.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX11548840/SRX11548840.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX11548840/SRX11548840.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 16:47:14: #1 read tag files... INFO @ Tue, 02 Aug 2022 16:47:14: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 16:47:20: 1000000 INFO @ Tue, 02 Aug 2022 16:47:21: 5000000 INFO @ Tue, 02 Aug 2022 16:47:25: 2000000 INFO @ Tue, 02 Aug 2022 16:47:29: 6000000 INFO @ Tue, 02 Aug 2022 16:47:30: 3000000 INFO @ Tue, 02 Aug 2022 16:47:36: 4000000 INFO @ Tue, 02 Aug 2022 16:47:36: 7000000 INFO @ Tue, 02 Aug 2022 16:47:41: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 16:47:44: 8000000 INFO @ Tue, 02 Aug 2022 16:47:44: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX11548840/SRX11548840.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX11548840/SRX11548840.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX11548840/SRX11548840.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX11548840/SRX11548840.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 16:47:44: #1 read tag files... INFO @ Tue, 02 Aug 2022 16:47:44: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 16:47:46: 6000000 INFO @ Tue, 02 Aug 2022 16:47:51: 9000000 INFO @ Tue, 02 Aug 2022 16:47:52: 1000000 INFO @ Tue, 02 Aug 2022 16:47:52: 7000000 INFO @ Tue, 02 Aug 2022 16:47:57: 8000000 INFO @ Tue, 02 Aug 2022 16:47:59: 10000000 INFO @ Tue, 02 Aug 2022 16:47:59: 2000000 INFO @ Tue, 02 Aug 2022 16:48:03: 9000000 INFO @ Tue, 02 Aug 2022 16:48:07: 3000000 INFO @ Tue, 02 Aug 2022 16:48:08: 11000000 INFO @ Tue, 02 Aug 2022 16:48:08: 10000000 INFO @ Tue, 02 Aug 2022 16:48:14: 11000000 INFO @ Tue, 02 Aug 2022 16:48:14: 4000000 INFO @ Tue, 02 Aug 2022 16:48:15: 12000000 INFO @ Tue, 02 Aug 2022 16:48:19: 12000000 INFO @ Tue, 02 Aug 2022 16:48:22: 5000000 INFO @ Tue, 02 Aug 2022 16:48:23: 13000000 INFO @ Tue, 02 Aug 2022 16:48:24: 13000000 INFO @ Tue, 02 Aug 2022 16:48:30: 6000000 INFO @ Tue, 02 Aug 2022 16:48:30: 14000000 INFO @ Tue, 02 Aug 2022 16:48:31: 14000000 INFO @ Tue, 02 Aug 2022 16:48:35: 15000000 INFO @ Tue, 02 Aug 2022 16:48:37: 7000000 INFO @ Tue, 02 Aug 2022 16:48:38: 15000000 INFO @ Tue, 02 Aug 2022 16:48:41: 16000000 INFO @ Tue, 02 Aug 2022 16:48:45: 8000000 INFO @ Tue, 02 Aug 2022 16:48:45: 16000000 INFO @ Tue, 02 Aug 2022 16:48:47: 17000000 INFO @ Tue, 02 Aug 2022 16:48:52: 9000000 INFO @ Tue, 02 Aug 2022 16:48:53: 17000000 INFO @ Tue, 02 Aug 2022 16:48:53: 18000000 INFO @ Tue, 02 Aug 2022 16:48:59: 19000000 INFO @ Tue, 02 Aug 2022 16:49:00: 10000000 INFO @ Tue, 02 Aug 2022 16:49:00: 18000000 INFO @ Tue, 02 Aug 2022 16:49:05: 20000000 INFO @ Tue, 02 Aug 2022 16:49:07: 11000000 INFO @ Tue, 02 Aug 2022 16:49:08: 19000000 INFO @ Tue, 02 Aug 2022 16:49:10: 21000000 INFO @ Tue, 02 Aug 2022 16:49:15: 12000000 INFO @ Tue, 02 Aug 2022 16:49:15: 20000000 INFO @ Tue, 02 Aug 2022 16:49:16: 22000000 INFO @ Tue, 02 Aug 2022 16:49:22: 23000000 INFO @ Tue, 02 Aug 2022 16:49:22: 13000000 INFO @ Tue, 02 Aug 2022 16:49:23: 21000000 INFO @ Tue, 02 Aug 2022 16:49:27: 24000000 INFO @ Tue, 02 Aug 2022 16:49:29: 14000000 INFO @ Tue, 02 Aug 2022 16:49:31: 22000000 INFO @ Tue, 02 Aug 2022 16:49:33: 25000000 INFO @ Tue, 02 Aug 2022 16:49:37: 15000000 INFO @ Tue, 02 Aug 2022 16:49:38: 23000000 INFO @ Tue, 02 Aug 2022 16:49:38: 26000000 INFO @ Tue, 02 Aug 2022 16:49:44: 27000000 INFO @ Tue, 02 Aug 2022 16:49:44: 16000000 INFO @ Tue, 02 Aug 2022 16:49:46: 24000000 INFO @ Tue, 02 Aug 2022 16:49:49: 28000000 INFO @ Tue, 02 Aug 2022 16:49:52: 17000000 INFO @ Tue, 02 Aug 2022 16:49:54: 25000000 INFO @ Tue, 02 Aug 2022 16:49:55: 29000000 INFO @ Tue, 02 Aug 2022 16:49:58: #1 tag size is determined as 75 bps INFO @ Tue, 02 Aug 2022 16:49:58: #1 tag size = 75 INFO @ Tue, 02 Aug 2022 16:49:58: #1 total tags in treatment: 14146233 INFO @ Tue, 02 Aug 2022 16:49:58: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 16:49:58: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 16:49:58: #1 tags after filtering in treatment: 13580518 INFO @ Tue, 02 Aug 2022 16:49:58: #1 Redundant rate of treatment: 0.04 INFO @ Tue, 02 Aug 2022 16:49:58: #1 finished! INFO @ Tue, 02 Aug 2022 16:49:58: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 16:49:58: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 16:49:59: #2 number of paired peaks: 76 WARNING @ Tue, 02 Aug 2022 16:49:59: Too few paired peaks (76) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 16:49:59: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX11548840/SRX11548840.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX11548840/SRX11548840.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX11548840/SRX11548840.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX11548840/SRX11548840.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 16:50:00: 18000000 INFO @ Tue, 02 Aug 2022 16:50:01: 26000000 INFO @ Tue, 02 Aug 2022 16:50:07: 19000000 INFO @ Tue, 02 Aug 2022 16:50:08: 27000000 INFO @ Tue, 02 Aug 2022 16:50:15: 20000000 INFO @ Tue, 02 Aug 2022 16:50:16: 28000000 INFO @ Tue, 02 Aug 2022 16:50:23: 21000000 INFO @ Tue, 02 Aug 2022 16:50:23: 29000000 INFO @ Tue, 02 Aug 2022 16:50:27: #1 tag size is determined as 75 bps INFO @ Tue, 02 Aug 2022 16:50:27: #1 tag size = 75 INFO @ Tue, 02 Aug 2022 16:50:27: #1 total tags in treatment: 14146233 INFO @ Tue, 02 Aug 2022 16:50:27: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 16:50:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 16:50:28: #1 tags after filtering in treatment: 13580518 INFO @ Tue, 02 Aug 2022 16:50:28: #1 Redundant rate of treatment: 0.04 INFO @ Tue, 02 Aug 2022 16:50:28: #1 finished! INFO @ Tue, 02 Aug 2022 16:50:28: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 16:50:28: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 16:50:28: #2 number of paired peaks: 76 WARNING @ Tue, 02 Aug 2022 16:50:28: Too few paired peaks (76) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 16:50:28: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX11548840/SRX11548840.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 6 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX11548840/SRX11548840.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX11548840/SRX11548840.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX11548840/SRX11548840.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 16:50:30: 22000000 INFO @ Tue, 02 Aug 2022 16:50:38: 23000000 INFO @ Tue, 02 Aug 2022 16:50:46: 24000000 INFO @ Tue, 02 Aug 2022 16:50:54: 25000000 INFO @ Tue, 02 Aug 2022 16:51:01: 26000000 INFO @ Tue, 02 Aug 2022 16:51:09: 27000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 02 Aug 2022 16:51:17: 28000000 INFO @ Tue, 02 Aug 2022 16:51:24: 29000000 INFO @ Tue, 02 Aug 2022 16:51:29: #1 tag size is determined as 75 bps INFO @ Tue, 02 Aug 2022 16:51:29: #1 tag size = 75 INFO @ Tue, 02 Aug 2022 16:51:29: #1 total tags in treatment: 14146233 INFO @ Tue, 02 Aug 2022 16:51:29: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 16:51:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 16:51:29: #1 tags after filtering in treatment: 13580518 INFO @ Tue, 02 Aug 2022 16:51:29: #1 Redundant rate of treatment: 0.04 INFO @ Tue, 02 Aug 2022 16:51:29: #1 finished! INFO @ Tue, 02 Aug 2022 16:51:29: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 16:51:29: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 16:51:30: #2 number of paired peaks: 76 WARNING @ Tue, 02 Aug 2022 16:51:30: Too few paired peaks (76) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 16:51:30: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX11548840/SRX11548840.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX11548840/SRX11548840.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX11548840/SRX11548840.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX11548840/SRX11548840.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。