Job ID = 16439658 SRX = SRX11499357 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 11317282 spots for SRR15192704/SRR15192704.sra Written 11317282 spots for SRR15192704/SRR15192704.sra Read 20765702 spots for SRR15192705/SRR15192705.sra Written 20765702 spots for SRR15192705/SRR15192705.sra fastq に変換しました。 bowtie でマッピング中... Your job 16441106 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 02:38:54 32082984 reads; of these: 32082984 (100.00%) were paired; of these: 5206821 (16.23%) aligned concordantly 0 times 20575861 (64.13%) aligned concordantly exactly 1 time 6300302 (19.64%) aligned concordantly >1 times ---- 5206821 pairs aligned concordantly 0 times; of these: 2573274 (49.42%) aligned discordantly 1 time ---- 2633547 pairs aligned 0 times concordantly or discordantly; of these: 5267094 mates make up the pairs; of these: 2316573 (43.98%) aligned 0 times 1236859 (23.48%) aligned exactly 1 time 1713662 (32.54%) aligned >1 times 96.39% overall alignment rate Time searching: 02:38:54 Overall time: 02:38:54 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 48 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 3143917 / 26629219 = 0.1181 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 19:10:54: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX11499357/SRX11499357.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX11499357/SRX11499357.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX11499357/SRX11499357.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX11499357/SRX11499357.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 19:10:54: #1 read tag files... INFO @ Tue, 02 Aug 2022 19:10:54: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 19:11:05: 1000000 INFO @ Tue, 02 Aug 2022 19:11:16: 2000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 19:11:23: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX11499357/SRX11499357.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX11499357/SRX11499357.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX11499357/SRX11499357.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX11499357/SRX11499357.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 19:11:23: #1 read tag files... INFO @ Tue, 02 Aug 2022 19:11:23: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 19:11:27: 3000000 INFO @ Tue, 02 Aug 2022 19:11:37: 1000000 INFO @ Tue, 02 Aug 2022 19:11:38: 4000000 INFO @ Tue, 02 Aug 2022 19:11:50: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 19:11:52: 2000000 INFO @ Tue, 02 Aug 2022 19:11:53: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX11499357/SRX11499357.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX11499357/SRX11499357.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX11499357/SRX11499357.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX11499357/SRX11499357.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 19:11:53: #1 read tag files... INFO @ Tue, 02 Aug 2022 19:11:53: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 19:12:03: 3000000 INFO @ Tue, 02 Aug 2022 19:12:06: 6000000 INFO @ Tue, 02 Aug 2022 19:12:09: 1000000 INFO @ Tue, 02 Aug 2022 19:12:15: 4000000 INFO @ Tue, 02 Aug 2022 19:12:20: 7000000 INFO @ Tue, 02 Aug 2022 19:12:24: 2000000 INFO @ Tue, 02 Aug 2022 19:12:30: 5000000 INFO @ Tue, 02 Aug 2022 19:12:34: 8000000 INFO @ Tue, 02 Aug 2022 19:12:40: 3000000 INFO @ Tue, 02 Aug 2022 19:12:43: 6000000 INFO @ Tue, 02 Aug 2022 19:12:47: 9000000 INFO @ Tue, 02 Aug 2022 19:12:55: 4000000 INFO @ Tue, 02 Aug 2022 19:12:56: 7000000 INFO @ Tue, 02 Aug 2022 19:13:00: 10000000 INFO @ Tue, 02 Aug 2022 19:13:09: 8000000 INFO @ Tue, 02 Aug 2022 19:13:12: 5000000 INFO @ Tue, 02 Aug 2022 19:13:13: 11000000 INFO @ Tue, 02 Aug 2022 19:13:21: 9000000 INFO @ Tue, 02 Aug 2022 19:13:27: 6000000 INFO @ Tue, 02 Aug 2022 19:13:28: 12000000 INFO @ Tue, 02 Aug 2022 19:13:33: 10000000 INFO @ Tue, 02 Aug 2022 19:13:42: 7000000 INFO @ Tue, 02 Aug 2022 19:13:44: 13000000 INFO @ Tue, 02 Aug 2022 19:13:44: 11000000 INFO @ Tue, 02 Aug 2022 19:13:57: 8000000 INFO @ Tue, 02 Aug 2022 19:13:59: 14000000 INFO @ Tue, 02 Aug 2022 19:14:00: 12000000 INFO @ Tue, 02 Aug 2022 19:14:12: 9000000 INFO @ Tue, 02 Aug 2022 19:14:12: 13000000 INFO @ Tue, 02 Aug 2022 19:14:13: 15000000 INFO @ Tue, 02 Aug 2022 19:14:24: 14000000 INFO @ Tue, 02 Aug 2022 19:14:26: 16000000 INFO @ Tue, 02 Aug 2022 19:14:28: 10000000 INFO @ Tue, 02 Aug 2022 19:14:35: 15000000 INFO @ Tue, 02 Aug 2022 19:14:39: 17000000 INFO @ Tue, 02 Aug 2022 19:14:43: 11000000 INFO @ Tue, 02 Aug 2022 19:14:47: 16000000 INFO @ Tue, 02 Aug 2022 19:14:53: 18000000 INFO @ Tue, 02 Aug 2022 19:14:57: 12000000 INFO @ Tue, 02 Aug 2022 19:14:58: 17000000 INFO @ Tue, 02 Aug 2022 19:15:08: 19000000 INFO @ Tue, 02 Aug 2022 19:15:10: 18000000 INFO @ Tue, 02 Aug 2022 19:15:12: 13000000 INFO @ Tue, 02 Aug 2022 19:15:21: 20000000 INFO @ Tue, 02 Aug 2022 19:15:21: 19000000 INFO @ Tue, 02 Aug 2022 19:15:27: 14000000 INFO @ Tue, 02 Aug 2022 19:15:33: 20000000 INFO @ Tue, 02 Aug 2022 19:15:34: 21000000 INFO @ Tue, 02 Aug 2022 19:15:41: 15000000 INFO @ Tue, 02 Aug 2022 19:15:44: 21000000 INFO @ Tue, 02 Aug 2022 19:15:47: 22000000 INFO @ Tue, 02 Aug 2022 19:15:56: 22000000 INFO @ Tue, 02 Aug 2022 19:15:56: 16000000 INFO @ Tue, 02 Aug 2022 19:16:01: 23000000 INFO @ Tue, 02 Aug 2022 19:16:07: 23000000 INFO @ Tue, 02 Aug 2022 19:16:11: 17000000 INFO @ Tue, 02 Aug 2022 19:16:16: 24000000 INFO @ Tue, 02 Aug 2022 19:16:18: 24000000 INFO @ Tue, 02 Aug 2022 19:16:26: 18000000 INFO @ Tue, 02 Aug 2022 19:16:29: 25000000 INFO @ Tue, 02 Aug 2022 19:16:29: 25000000 INFO @ Tue, 02 Aug 2022 19:16:40: 26000000 INFO @ Tue, 02 Aug 2022 19:16:41: 19000000 INFO @ Tue, 02 Aug 2022 19:16:42: 26000000 INFO @ Tue, 02 Aug 2022 19:16:51: 27000000 INFO @ Tue, 02 Aug 2022 19:16:54: 27000000 INFO @ Tue, 02 Aug 2022 19:16:56: 20000000 INFO @ Tue, 02 Aug 2022 19:17:04: 28000000 INFO @ Tue, 02 Aug 2022 19:17:07: 28000000 INFO @ Tue, 02 Aug 2022 19:17:11: 21000000 INFO @ Tue, 02 Aug 2022 19:17:15: 29000000 INFO @ Tue, 02 Aug 2022 19:17:20: 29000000 INFO @ Tue, 02 Aug 2022 19:17:25: 22000000 INFO @ Tue, 02 Aug 2022 19:17:27: 30000000 INFO @ Tue, 02 Aug 2022 19:17:33: 30000000 INFO @ Tue, 02 Aug 2022 19:17:40: 31000000 INFO @ Tue, 02 Aug 2022 19:17:40: 23000000 INFO @ Tue, 02 Aug 2022 19:17:46: 31000000 INFO @ Tue, 02 Aug 2022 19:17:52: 32000000 INFO @ Tue, 02 Aug 2022 19:17:55: 24000000 INFO @ Tue, 02 Aug 2022 19:17:59: 32000000 INFO @ Tue, 02 Aug 2022 19:18:04: 33000000 INFO @ Tue, 02 Aug 2022 19:18:09: 25000000 INFO @ Tue, 02 Aug 2022 19:18:12: 33000000 INFO @ Tue, 02 Aug 2022 19:18:17: 34000000 INFO @ Tue, 02 Aug 2022 19:18:23: 26000000 INFO @ Tue, 02 Aug 2022 19:18:26: 34000000 INFO @ Tue, 02 Aug 2022 19:18:30: 35000000 INFO @ Tue, 02 Aug 2022 19:18:38: 27000000 INFO @ Tue, 02 Aug 2022 19:18:38: 35000000 INFO @ Tue, 02 Aug 2022 19:18:42: 36000000 INFO @ Tue, 02 Aug 2022 19:18:51: 36000000 INFO @ Tue, 02 Aug 2022 19:18:52: 28000000 INFO @ Tue, 02 Aug 2022 19:18:53: 37000000 INFO @ Tue, 02 Aug 2022 19:19:03: 37000000 INFO @ Tue, 02 Aug 2022 19:19:05: 38000000 INFO @ Tue, 02 Aug 2022 19:19:07: 29000000 INFO @ Tue, 02 Aug 2022 19:19:16: 38000000 INFO @ Tue, 02 Aug 2022 19:19:18: 39000000 INFO @ Tue, 02 Aug 2022 19:19:23: 30000000 INFO @ Tue, 02 Aug 2022 19:19:30: 39000000 INFO @ Tue, 02 Aug 2022 19:19:30: 40000000 INFO @ Tue, 02 Aug 2022 19:19:37: 31000000 INFO @ Tue, 02 Aug 2022 19:19:43: 41000000 INFO @ Tue, 02 Aug 2022 19:19:43: 40000000 INFO @ Tue, 02 Aug 2022 19:19:52: 32000000 INFO @ Tue, 02 Aug 2022 19:19:55: 42000000 INFO @ Tue, 02 Aug 2022 19:19:56: 41000000 INFO @ Tue, 02 Aug 2022 19:20:06: 33000000 INFO @ Tue, 02 Aug 2022 19:20:08: 43000000 INFO @ Tue, 02 Aug 2022 19:20:10: 42000000 INFO @ Tue, 02 Aug 2022 19:20:20: 34000000 INFO @ Tue, 02 Aug 2022 19:20:21: 44000000 INFO @ Tue, 02 Aug 2022 19:20:24: 43000000 INFO @ Tue, 02 Aug 2022 19:20:33: 45000000 INFO @ Tue, 02 Aug 2022 19:20:34: 35000000 INFO @ Tue, 02 Aug 2022 19:20:37: 44000000 INFO @ Tue, 02 Aug 2022 19:20:46: 46000000 INFO @ Tue, 02 Aug 2022 19:20:49: 36000000 INFO @ Tue, 02 Aug 2022 19:20:51: 45000000 INFO @ Tue, 02 Aug 2022 19:20:58: 47000000 INFO @ Tue, 02 Aug 2022 19:21:03: 37000000 INFO @ Tue, 02 Aug 2022 19:21:04: 46000000 INFO @ Tue, 02 Aug 2022 19:21:10: 48000000 INFO @ Tue, 02 Aug 2022 19:21:17: 38000000 INFO @ Tue, 02 Aug 2022 19:21:17: 47000000 INFO @ Tue, 02 Aug 2022 19:21:23: 49000000 INFO @ Tue, 02 Aug 2022 19:21:31: 48000000 INFO @ Tue, 02 Aug 2022 19:21:31: 39000000 INFO @ Tue, 02 Aug 2022 19:21:35: 50000000 INFO @ Tue, 02 Aug 2022 19:21:44: 49000000 INFO @ Tue, 02 Aug 2022 19:21:45: 40000000 INFO @ Tue, 02 Aug 2022 19:21:48: 51000000 INFO @ Tue, 02 Aug 2022 19:21:57: 50000000 INFO @ Tue, 02 Aug 2022 19:21:59: 52000000 INFO @ Tue, 02 Aug 2022 19:22:00: 41000000 INFO @ Tue, 02 Aug 2022 19:22:11: 51000000 INFO @ Tue, 02 Aug 2022 19:22:11: 53000000 INFO @ Tue, 02 Aug 2022 19:22:15: 42000000 INFO @ Tue, 02 Aug 2022 19:22:24: 52000000 INFO @ Tue, 02 Aug 2022 19:22:24: 54000000 INFO @ Tue, 02 Aug 2022 19:22:30: 43000000 INFO @ Tue, 02 Aug 2022 19:22:37: 55000000 INFO @ Tue, 02 Aug 2022 19:22:37: 53000000 INFO @ Tue, 02 Aug 2022 19:22:44: #1 tag size is determined as 144 bps INFO @ Tue, 02 Aug 2022 19:22:44: #1 tag size = 144 INFO @ Tue, 02 Aug 2022 19:22:44: #1 total tags in treatment: 23885996 INFO @ Tue, 02 Aug 2022 19:22:44: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 19:22:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 19:22:44: 44000000 INFO @ Tue, 02 Aug 2022 19:22:44: #1 tags after filtering in treatment: 21914517 INFO @ Tue, 02 Aug 2022 19:22:44: #1 Redundant rate of treatment: 0.08 INFO @ Tue, 02 Aug 2022 19:22:44: #1 finished! INFO @ Tue, 02 Aug 2022 19:22:44: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 19:22:44: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 19:22:46: #2 number of paired peaks: 177 WARNING @ Tue, 02 Aug 2022 19:22:46: Fewer paired peaks (177) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 177 pairs to build model! INFO @ Tue, 02 Aug 2022 19:22:46: start model_add_line... INFO @ Tue, 02 Aug 2022 19:22:46: start X-correlation... INFO @ Tue, 02 Aug 2022 19:22:46: end of X-cor INFO @ Tue, 02 Aug 2022 19:22:46: #2 finished! INFO @ Tue, 02 Aug 2022 19:22:46: #2 predicted fragment length is 239 bps INFO @ Tue, 02 Aug 2022 19:22:46: #2 alternative fragment length(s) may be 239 bps INFO @ Tue, 02 Aug 2022 19:22:46: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX11499357/SRX11499357.10_model.r WARNING @ Tue, 02 Aug 2022 19:22:46: #2 Since the d (239) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 02 Aug 2022 19:22:46: #2 You may need to consider one of the other alternative d(s): 239 WARNING @ Tue, 02 Aug 2022 19:22:46: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 02 Aug 2022 19:22:46: #3 Call peaks... INFO @ Tue, 02 Aug 2022 19:22:46: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 02 Aug 2022 19:22:50: 54000000 INFO @ Tue, 02 Aug 2022 19:22:58: 45000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 02 Aug 2022 19:23:04: 55000000 INFO @ Tue, 02 Aug 2022 19:23:11: 46000000 INFO @ Tue, 02 Aug 2022 19:23:12: #1 tag size is determined as 144 bps INFO @ Tue, 02 Aug 2022 19:23:12: #1 tag size = 144 INFO @ Tue, 02 Aug 2022 19:23:12: #1 total tags in treatment: 23885996 INFO @ Tue, 02 Aug 2022 19:23:12: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 19:23:12: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 19:23:12: #1 tags after filtering in treatment: 21914517 INFO @ Tue, 02 Aug 2022 19:23:12: #1 Redundant rate of treatment: 0.08 INFO @ Tue, 02 Aug 2022 19:23:12: #1 finished! INFO @ Tue, 02 Aug 2022 19:23:12: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 19:23:12: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 19:23:14: #2 number of paired peaks: 177 WARNING @ Tue, 02 Aug 2022 19:23:14: Fewer paired peaks (177) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 177 pairs to build model! INFO @ Tue, 02 Aug 2022 19:23:14: start model_add_line... INFO @ Tue, 02 Aug 2022 19:23:14: start X-correlation... INFO @ Tue, 02 Aug 2022 19:23:14: end of X-cor INFO @ Tue, 02 Aug 2022 19:23:14: #2 finished! INFO @ Tue, 02 Aug 2022 19:23:14: #2 predicted fragment length is 239 bps INFO @ Tue, 02 Aug 2022 19:23:14: #2 alternative fragment length(s) may be 239 bps INFO @ Tue, 02 Aug 2022 19:23:14: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX11499357/SRX11499357.05_model.r WARNING @ Tue, 02 Aug 2022 19:23:14: #2 Since the d (239) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 02 Aug 2022 19:23:14: #2 You may need to consider one of the other alternative d(s): 239 WARNING @ Tue, 02 Aug 2022 19:23:14: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 02 Aug 2022 19:23:14: #3 Call peaks... INFO @ Tue, 02 Aug 2022 19:23:14: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 02 Aug 2022 19:23:23: 47000000 INFO @ Tue, 02 Aug 2022 19:23:26: #3 Call peaks for each chromosome... INFO @ Tue, 02 Aug 2022 19:23:35: 48000000 INFO @ Tue, 02 Aug 2022 19:23:46: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX11499357/SRX11499357.10_peaks.xls INFO @ Tue, 02 Aug 2022 19:23:46: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX11499357/SRX11499357.10_peaks.narrowPeak INFO @ Tue, 02 Aug 2022 19:23:46: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX11499357/SRX11499357.10_summits.bed INFO @ Tue, 02 Aug 2022 19:23:46: Done! pass1 - making usageList (15 chroms): 2 millis pass2 - checking and writing primary data (2848 records, 4 fields): 9 millis CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 19:23:49: 49000000 INFO @ Tue, 02 Aug 2022 19:23:53: #3 Call peaks for each chromosome... INFO @ Tue, 02 Aug 2022 19:24:01: 50000000 INFO @ Tue, 02 Aug 2022 19:24:13: 51000000 INFO @ Tue, 02 Aug 2022 19:24:13: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX11499357/SRX11499357.05_peaks.xls INFO @ Tue, 02 Aug 2022 19:24:13: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX11499357/SRX11499357.05_peaks.narrowPeak INFO @ Tue, 02 Aug 2022 19:24:14: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX11499357/SRX11499357.05_summits.bed INFO @ Tue, 02 Aug 2022 19:24:14: Done! pass1 - making usageList (15 chroms): 2 millis pass2 - checking and writing primary data (4818 records, 4 fields): 9 millis CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 19:24:25: 52000000 INFO @ Tue, 02 Aug 2022 19:24:37: 53000000 INFO @ Tue, 02 Aug 2022 19:24:50: 54000000 INFO @ Tue, 02 Aug 2022 19:25:03: 55000000 BigWig に変換しました。 INFO @ Tue, 02 Aug 2022 19:25:10: #1 tag size is determined as 144 bps INFO @ Tue, 02 Aug 2022 19:25:10: #1 tag size = 144 INFO @ Tue, 02 Aug 2022 19:25:10: #1 total tags in treatment: 23885996 INFO @ Tue, 02 Aug 2022 19:25:10: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 19:25:10: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 19:25:11: #1 tags after filtering in treatment: 21914517 INFO @ Tue, 02 Aug 2022 19:25:11: #1 Redundant rate of treatment: 0.08 INFO @ Tue, 02 Aug 2022 19:25:11: #1 finished! INFO @ Tue, 02 Aug 2022 19:25:11: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 19:25:11: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 19:25:12: #2 number of paired peaks: 177 WARNING @ Tue, 02 Aug 2022 19:25:12: Fewer paired peaks (177) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 177 pairs to build model! INFO @ Tue, 02 Aug 2022 19:25:12: start model_add_line... INFO @ Tue, 02 Aug 2022 19:25:12: start X-correlation... INFO @ Tue, 02 Aug 2022 19:25:13: end of X-cor INFO @ Tue, 02 Aug 2022 19:25:13: #2 finished! INFO @ Tue, 02 Aug 2022 19:25:13: #2 predicted fragment length is 239 bps INFO @ Tue, 02 Aug 2022 19:25:13: #2 alternative fragment length(s) may be 239 bps INFO @ Tue, 02 Aug 2022 19:25:13: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX11499357/SRX11499357.20_model.r WARNING @ Tue, 02 Aug 2022 19:25:13: #2 Since the d (239) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 02 Aug 2022 19:25:13: #2 You may need to consider one of the other alternative d(s): 239 WARNING @ Tue, 02 Aug 2022 19:25:13: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 02 Aug 2022 19:25:13: #3 Call peaks... INFO @ Tue, 02 Aug 2022 19:25:13: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 02 Aug 2022 19:25:52: #3 Call peaks for each chromosome... INFO @ Tue, 02 Aug 2022 19:26:12: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX11499357/SRX11499357.20_peaks.xls INFO @ Tue, 02 Aug 2022 19:26:12: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX11499357/SRX11499357.20_peaks.narrowPeak INFO @ Tue, 02 Aug 2022 19:26:12: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX11499357/SRX11499357.20_summits.bed INFO @ Tue, 02 Aug 2022 19:26:12: Done! pass1 - making usageList (14 chroms): 1 millis pass2 - checking and writing primary data (1531 records, 4 fields): 5 millis CompletedMACS2peakCalling